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    METHODS AND KITS FOR EXTRACTING NUCLEIC ACIDS FROM PARAFFIN EMBEDDED SAMPLES
    4.
    发明专利
    METHODS AND KITS FOR EXTRACTING NUCLEIC ACIDS FROM PARAFFIN EMBEDDED SAMPLES 未知

    公开(公告)号：SG11202001088PA

    公开(公告)日：2020-03-30

    申请号：SG11202001088P

    申请日：2018-08-20

    Applicant: FLUIDIGM CORP

    Inventor： QIN JIAN ,  CHEN PEILIN ,  RAMAKRISHNAN RAMESH

    IPC: C12N15/10 ,  C12Q1/6806

    Abstract: This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.

    METHODS AND KITS FOR EXTRACTING NUCLEIC ACIDS FROM PARAFFIN EMBEDDED SAMPLES
    5.
    发明专利
    METHODS AND KITS FOR EXTRACTING NUCLEIC ACIDS FROM PARAFFIN EMBEDDED SAMPLES 未知

    公开(公告)号：CA3072463A1

    公开(公告)日：2019-02-21

    申请号：CA3072463

    申请日：2018-08-20

    Applicant: FLUIDIGM CORP

    Inventor： QIN JIAN ,  CHEN PEILIN ,  RAMAKRISHNAN RAMESH

    IPC: C12N15/10 ,  C12Q1/6806

    Abstract: Disclosed herein is a method and kit thereof for isolating nucleic acids from wax-embedded samples using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids using ethanol, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.

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