公开(公告)号：WO2015148187A1

公开(公告)日：2015-10-01

申请号：PCT/US2015/021037

申请日：2015-03-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： WILLIAMS, Trevor G.

IPC: B01F7/00 ,  B01F15/00

CPC classification number: B01F15/00681 ,  B01F7/001 ,  B01F7/00675 ,  B01F7/00691 ,  B01F15/0085 ,  B01F2215/0073

Abstract: A fluid mixing system includes a flexible bag (12) having an interior surface bounding a compartment. An elongated first tubular connector (80) is disposed within the compartment of the flexible bag, the first tubular connector having a first end and an opposing second end, the first end of the first tubular connector being coupled with the flexible bag. An elongated second tubular connector (84) is disposed within the compartment of the flexible bag, the second tubular connector being more rigid than the first tubular connector and having a first end coupled to the second end of the first tubular connector. A plurality of impellers (85A-C) are disposed on the elongated second tubular connector at spaced apart locations.

Abstract translation: 流体混合系统包括具有限定隔室的内表面的柔性袋（12）。 细长的第一管状连接器（80）设置在柔性袋的隔室内，第一管状连接器具有第一端和相对的第二端，第一管状连接器的第一端与柔性袋结合。 细长的第二管状连接器（84）设置在柔性袋的隔室内，第二管状连接器比第一管状连接器更刚性并且具有联接到第一管状连接器的第二端的第一端。 多个叶轮（85A-C）在间隔开的位置设置在细长的第二管状连接器上。

公开(公告)号：WO2015134139A1

公开(公告)日：2015-09-11

申请号：PCT/US2015/014210

申请日：2015-02-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： KADUCHAK, Gregory ,  AKERLUND, Jace ,  MALKIN, Jason ,  WARD, Michael

IPC: G01N15/14

CPC classification number: G01N15/1012 ,  G01N15/1404 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1018 ,  G01N2015/1068 ,  G01N2015/1075 ,  G01N2015/1438

Abstract: The present set of embodiments relates to systems and methods for diagnosing a fluidics system and determining data processing settings for a flow cytometer. Systems and methods for diagnosing a fluidics system require accurate measurement and interpretation of fluctuations within the fluid delivery system. Systems and methods for determining data processing settings require an accurate measurement of peak times among various channels and being able to adjust time delay settings wherein peak time is the measurement of time elapsed from the beginning of the data collection time window to the highest peak in the window.

Abstract translation: 本组实施例涉及用于诊断流体系统并确定流式细胞仪的数据处理设置的系统和方法。 用于诊断流体系统的系统和方法需要准确测量和解释流体输送系统内的波动。 用于确定数据处理设置的系统和方法需要精确测量各种通道中的峰值时间，并且能够调整时间延迟设置，其中峰值时间是从数据采集时间窗口开始到数据采集时间窗口的最高峰值的时间的测量 窗口。

公开(公告)号：WO2015126589A1

公开(公告)日：2015-08-27

申请号：PCT/US2015/013481

申请日：2015-01-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LABARGE, Shawn ,  MOORE, Rebecca ,  ALESSI, Daniel ,  HASSETT, Richard ,  REZAC, Peter ,  NIELSEN, Karsten ,  ANDERSON, Neal ,  DONZE, Tom ,  CLARK, Elizabeth

IPC: C12N5/00 ,  C12M1/00

CPC classification number: C12M23/28 ,  C12M23/26 ,  C12M23/40 ,  C12M23/52 ,  C12M29/04 ,  C12M29/06 ,  C12M29/20 ,  C12M29/26 ,  C12M41/48 ,  C12M99/00

Abstract: The present set of embodiments relate to a system, method, and apparatus for hydrating and mixing a liquid medium from a dry medium. Such systems, methods, and apparatuses can be used in the biotech industry because they can be used to provide prepackaged dry media in a format that can be quickly rehydrated free of issues with operator error found in more conventional media rehydration systems using mixing tanks and reactors.

Abstract translation: 本组实施例涉及用于从干介质中水合和混合液体介质的系统，方法和装置。 这样的系统，方法和装置可以用于生物技术行业，因为它们可以用于以可以快速再水化的形式提供预先包装的干燥介质，而不需要在使用混合罐和反应器的更常规的介质再水合系统中发现的操作者错误的问题 。

公开(公告)号：WO2015109192A1

公开(公告)日：2015-07-23

申请号：PCT/US2015/011769

申请日：2015-01-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： KJAR, Steven R. ,  BROWN, Jeffery O. ,  BROWN, Jason D. ,  BRAU, Christopher D.

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/06 ,  C12M1/26 ,  C12M1/21

CPC classification number: G01N27/02 ,  C12M21/00 ,  C12M23/14 ,  C12M23/26 ,  C12M23/48 ,  C12M27/02 ,  C12M29/06 ,  C12M33/04 ,  C12M41/02 ,  C12M41/48 ,  C12N13/00 ,  C12Q3/00 ,  G01F23/24 ,  G01R1/04

Abstract: A foam sensor system includes a flexible bag comprised of a polymeric material and bounding a compartment. A foam sensor assembly is mounted on the flexible bag and includes a foam contact disposed within the compartment of the flexible bag. A ground assembly is mounted on the flexible bag and includes a ground contact disposed within the compartment of the flexible bag, the foam sensor assembly and the ground assembly being configured so that an electrical potential can be applied between the foam contact and the ground contact.

Abstract translation: 泡沫传感器系统包括由聚合物材料构成并包围隔间的柔性袋。 泡沫传感器组件安装在柔性袋上并且包括布置在柔性袋的隔室内的泡沫接触件。 接地组件安装在柔性袋上，并且包括设置在柔性袋的隔室内的接地触头，泡沫传感器组件和接地组件被配置为使得电位可以施加在泡沫触头和接地触点之间。

公开(公告)号：WO2015061714A1

公开(公告)日：2015-04-30

申请号：PCT/US2014/062222

申请日：2014-10-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： ANGRISH, Parul ,  YANG, Zhiwei

IPC: C07D207/16 ,  C12Q1/68 ,  C12Q1/686

CPC classification number: C12Q1/686 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C07D207/16 ,  C07D211/60 ,  C11D1/88 ,  C12N9/1252 ,  C12N9/96

Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.

Abstract translation: 本公开涉及用于各种组合物，试剂盒和方法的新化合物，包括例如在聚合酶存储缓冲液中的用途，以及核酸合成或扩增反应如聚合酶链反应（PCR）。 还描述了制备新化合物的方法。

公开(公告)号：WO2015050919A1

公开(公告)日：2015-04-09

申请号：PCT/US2014/058517

申请日：2014-10-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： GOTTIMUKKALA, Rajesh ,  HYLAND, Fiona ,  UTIRAMERUR, Sowmi ,  SCHAGEMAN, Jeoffrey ,  MAGDALENO, Susan

IPC: C12Q1/68

CPC classification number: G06F19/18 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q2535/101 ,  C12Q2537/143 ,  C12Q2539/105

Abstract: Systems and method for identifying long deletions can obtain sequencing information for a plurality of amplicons in and around a potential region from a nucleic acid sample. The sequencing information can include a plurality of reads that can be mapped to a reference sequence. Using information, such as where reads map to a reference sequence and relative abundance of reads for the amplicons, structural variants can be identified and a determination can be made if the nucleic acid sample is homozygous or heterozygous for the structural variant.

Abstract translation: 用于识别长缺失的系统和方法可以获得核酸样品的潜在区域内和周围的多个扩增子的测序信息。 排序信息可以包括可以映射到参考序列的多个读取。 使用诸如读数映射到扩增子的参考序列和相对丰度的信息的信息，可以鉴定结构变体，并且如果核酸样品对于结构变体是纯合的或杂合的，则可以进行确定。

公开(公告)号：WO2015038954A1

公开(公告)日：2015-03-19

申请号：PCT/US2014/055485

申请日：2014-09-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LUO, Guobin ,  SEDOVA, Marina ,  LIGHT, David ,  JONES, Ryan ,  ALANJARY, Mohammad

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2563/149 ,  C12Q2563/137 ,  C12Q2535/122

Abstract: A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.

Abstract translation: 对核酸链进行测序的方法包括接收具有与聚合物基质偶联的核酸链的颗粒，将颗粒暴露于包含缩合剂的溶液中，并将颗粒施用于表面，颗粒沉积在表面上。

公开(公告)号：WO2014165710A2

公开(公告)日：2014-10-09

申请号：PCT/US2014/032888

申请日：2014-04-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： RHODES, Daniel ,  SADIS, Seth ,  WYNGAARD, Peter ,  BANKHEAD, Armand ,  CYANAM, Dinesh ,  KHAZANOV, Nikolay

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/156

Abstract: The disclosure provides gene fusion variants and novel associations with disease states, as well as kits, probes, and methods of using the same.

Abstract translation: 本公开提供了基因融合变体和与疾病状态的新型关联，以及试剂盒，探针及其使用方法。

公开(公告)号：WO2014165210A9

公开(公告)日：2014-10-09

申请号：PCT/US2014/024803

申请日：2014-03-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LI, Kelly ,  DONG, Shoulian ,  CHEN, Caifu

IPC: C12Q1/68

Abstract: The present disclosure is drawn to methods for detection, quantitation and analysis of nucleotides of interest, for example SNPs, in nucleic acid sequences of interest using universal FRET-based reporter primers.

公开(公告)号：WO2014151955A1

公开(公告)日：2014-09-25

申请号：PCT/US2014/026724

申请日：2014-03-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： SADIS, Seth ,  WILLIAMS, Paul, Douglas

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/156

Abstract: Prognosis methods for predicting clinical outcome for a human subject diagnosed with squamous cell lung carcinoma using a panel of molecular markers that includes CDKN2A and CCND1. CDKN2A / p16 deletion or loss of function mutation and CCND1 amplification are related to the subject's increased likelihood of a negative clinical outcome.

Abstract translation: 使用包括CDKN2A和CCND1的分子标记的一组来预测诊断为鳞状细胞肺癌的人类受试者的临床结果的预后方法。 CDKN2A / p16缺失或缺失功能突变和CCND1扩增与受试者增加阴性临床结局的可能性有关。