公开(公告)号：WO9509176A1

公开(公告)日：1995-04-06

申请号：PCT/US9309294

申请日：1993-09-28

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  MATSON ROBERT S ,  RAMPAL JANG B

IPC: C07H21/00 ,  C08F8/00 ,  C08F8/32 ,  C12Q1/68 ,  C07K1/04 ,  C08F8/30 ,  C08J3/28 ,  G01N33/68

CPC classification number: C07H21/00 ,  C08F8/30 ,  C08F8/32 ,  C12Q1/6827 ,  C12Q1/6834 ,  C08F110/06

Abstract: Disclosed herein are surface activated, organic polymers useful for biopolymer synthesis. Most preferably, aminated polypropylene is used for the synthesis of oligonucleotides thereto, and these devices are most preferably utilized for genetic analysis of patient samples.

Abstract translation: 本文公开了可用于生物聚合物合成的表面活化的有机聚合物。 最优选地，胺化聚丙烯用于合成寡核苷酸，并且这些装置最优选用于患者样品的遗传分析。

公开(公告)号：WO1994029713A1

公开(公告)日：1994-12-22

申请号：PCT/US1994004514

申请日：1994-04-25

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GOODALE, David, L. ,  REEVES, George, I.

IPC: G01N27/447

CPC classification number: G01N27/44782 ,  G01N27/44704

Abstract: A capillary and capillary retaining system including a capillary assembly (20) having first and second end holders (32, 36). The first and second end holders (32, 36) are adapted to be received by first and second retainers (22, 24). The first end holder (32) may include protruding portions (48, 50) and the first end retainer includes clips to receive the protruding portions. The second end holder may include opposite recesses and the second retainer is adapted to receive optical cables that are received within the recesses to retain the second end holder. Locks retained by the second end retainer cooperate with grooves in the optical cables to retain the optical cables.

Abstract translation: 毛细管和毛细管保持系统，其包括具有第一和第二端部保持器（32,36）的毛细管组件（20）。 第一和第二端部保持器（32,36）适于被第一和第二保持器（22,24）接收。 第一端部支架（32）可以包括突出部分（48,50），并且第一端部保持器包括用于容纳突出部分的夹子。 第二端保持器可以包括相对的凹部，并且第二保持器适于容纳容纳在凹部内以保持第二端保持器的光缆。 由第二端保持器保持的锁与光缆中的凹槽配合以保持光缆。

公开(公告)号：WO9424312A3

公开(公告)日：1994-12-08

申请号：PCT/US9404275

申请日：1994-04-19

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  KONRAD KENNETH D ,  RAMPAL JANG B ,  COOK RONALD M

IPC: C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C07H21/00 ,  C12N15/1034 ,  C12Q1/6853 ,  C12Q1/6862

Abstract: Disclosed herein, inter alia, are Inverse Linkage Oligonucleotides ('ILO') useful in enzymatic process. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.

公开(公告)号：WO1994022585A1

公开(公告)日：1994-10-13

申请号：PCT/US1994003596

申请日：1994-04-01

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  MOORE, Patrick, Q. ,  SCHIESSLER, David, L.

IPC: B04B05/04

CPC classification number: B65D39/0088 ,  B01L3/5021 ,  B04B5/0414 ,  B65D2539/006

Abstract: The present invention directed to a plug (16) for sealing a centrifuge tube (10). The plug (16) of the present invention is shaped and sized to provide an interference fit between the plug (16) and the tube stem (12) of the centrifuge tube. In the described embodiment, the plug is configured with a tapered body narrowing to a flared end (22). To facilitate the insertion and removal of the plug with moderate force, without compromising the restraining capability of the interference fit, the area of interference contact between the flared end and the filler stem is strategically reduced. An o-ring is provided in an annular groove (20) around the tapered body. The flared end (22) creates an interference fit with a tapered filler stem (12) whereby the plug (16) is secured in the filler stem (12) with a snapping action when the flared end (22) of the plug (16) extends into the tube beyond the tapered filler stem (12). In this snap-in position, the plug (16) is secured in the filler stem (12), providing an initial seal; such seal increases upon centrifugation by a self-sealing mechanism, either attributed to the internal hydrostatic pressure in the tube and/or the force of a support spacer on the plug. After centrifugation, the snap coupling between the filler stem and the plug securely retains the plug against any residual internal pressure built up within the tube created by deformation of the centrifuge tube either from centrifugation or through handling of the tube by the user.

Abstract translation: 本发明涉及用于密封离心管（10）的塞子（16）。 本发明的插头（16）的形状和尺寸被设计成提供离心管的插塞（16）和管杆（12）之间的过盈配合。 在所描述的实施例中，插头构造成具有朝向扩张端（22）变窄的锥形体。 为了有利于以适度的力插入和移除插头，在不影响过盈配合的约束能力的情况下，战略上减少了扩口端与加注杆之间的干涉接触面积。 O形圈设置在围绕锥形主体的环形槽（20）中。 扩口端（22）产生与锥形填充杆（12）的过盈配合，由此当插头（16）的扩口端（22）具有卡扣动作时，插头（16）固定在填充杆（12） 延伸到超过锥形填充杆（12）的管中。 在该卡入位置中，插头（16）固定在加注杆（12）中，提供初始密封; 这种密封通过自密封机构进行离心时增加，归因于管中的内部静水压力和/或插塞上的支撑间隔件的力。 离心后，填充杆和插塞之间的卡扣联结可以牢固地保持插塞抵抗由离心管变形而产生的管内的任何残余内部压力，或者离心或通过使用者处理管。

公开(公告)号：WO1994017401A1

公开(公告)日：1994-08-04

申请号：PCT/US1994000762

申请日：1994-01-19

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  HERRICK, Steven, S. ,  STERNBERG, James, C.

IPC: G01N27/447

CPC classification number: G01N27/44795 ,  G01N27/44752

Abstract: The present invention provides processes for isoelectric focusing ("IEF") and associated detection, which incorporates a dynamic means of electroosmotic flow ("EOF") control during IEF and/or after IEF to effect solute mobilization. In accordance with the present invention, the EOF control during IEF and/or solute mobilization after IEF are accomplished by applying an external electric field, relative to an internal electric field, to modify the electroosmotic flow in the capillary. This can be done by disposing a conductive member at one or more locations outside and along the buffer column in the capillary. The conductive member may be statically charged or caused to conduct a current to create the external required electric field. The applied external electric field may be adjusted, relative to the external electric field, during IEF necessary to reduce or completely suppress EOF to prevent flow of the buffer. Upon the completion of IEF (irrespective of the method of reducing or removing EOF during IEF), the external electric field is adjusted, relative to the internal electric field, such that the buffer carrying the focused solutes are moved electroosmotically through the capillary past a detection point. The present invention is applicable to internally coated capillary which suppresses EOF even in the absence of the external electric field.

Abstract translation: 本发明提供等电聚焦（“IEF”）和相关检测的方法，其包括在IEF期间和/或在IEF之后的电渗流（“EOF”）控制的动态方式以进行溶质动员。 根据本发明，在IEF之间的IEF和/或溶质动员期间的EOF控制是通过相对于内部电场施加外部电场来实现的，以改变毛细管中的电渗流。 这可以通过在毛细管中的缓冲柱的外部和沿着一个或多个位置设置导电构件来实现。 导电构件可以被静电充电或导致电流以产生外部所需的电场。 在IEF期间，可以相对于外部电场调整所施加的外部电场，以减少或完全抑制EOF以防止缓冲器的流动。 在完成IEF（不管在IEF期间减少或去除EOF的方法）时，相对于内部电场调节外部电场，使得携带聚焦溶质的缓冲液通过毛细管电动移动通过检测 点。 本发明适用于即使没有外部电场也能抑制EOF的内部涂布毛细管。

公开(公告)号：WO1994015637A2

公开(公告)日：1994-07-21

申请号：PCT/US1993012187

申请日：1993-12-13

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  CERCEK, Boris ,  CERCEK, Lea

IPC: A61K39/395

CPC classification number: G01N33/5091 ,  A61K35/16 ,  A61K38/00 ,  A61K47/6845 ,  C07K7/08 ,  C07K14/4745 ,  C07K14/585 ,  C07K16/18 ,  G01N33/5011

Abstract: A method for blocking suppression of at least one of the natural killer (NK) and lymphocyte activated killer (LAK) immune defense mechanisms in lymphocytes of cancer patients comprises administering to a cancer patient an agent capable of blocking the immune defense suppressive activities of a peptide capable of inducing a detectable decrease in the structuredness of the cytoplasmic matrix in lymphocytes isolated from a patient with cancer (an SCM-factor peptide) in a quantity sufficient to block suppression of at least one of the natural killer (NK) and lymphocyte activated killer (LAK) immune defense mechanisms. The agent can comprise an antibody, an antisense peptide, or a factor present in blood plasma. The invention also includes pharmaceutical compositions for blocking suppression of immune defense and kits for stimulating immune response of cancer patients, as well as immunization methods to induce antibody to SCM-factor peptides.

Abstract translation: 抑制癌症患者淋巴细胞中的至少一种天然杀伤（NK）和淋巴细胞活化的杀伤（LAK）免疫防御机制的抑制方法包括向癌症患者施用能够阻断肽的免疫防御抑制活性的试剂 能够诱导分离自患有癌症的淋巴细胞（SCM-因子肽）中的足以阻断至少一种天然杀伤（NK）和淋巴细胞活化的杀伤剂的抑制的量的淋巴细胞的细胞质基质的结构化的可检测的降低 （LAK）免疫防御机制。 该试剂可以包含抗体，反义肽或存在于血浆中的因子。 本发明还包括用于阻断免疫防御的药物组合物和用于刺激癌症患者的免疫应答的试剂盒，以及用于诱导针对SCM-因子肽的抗体的免疫方法。

公开(公告)号：WO1994001214A1

公开(公告)日：1994-01-20

申请号：PCT/US1993006217

申请日：1993-06-29

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  KEENAN, Charles, A. ,  COASSIN, Peter, J. ,  HELPHREY, David, B. ,  WINER, Roger ,  DIH, Jiunn-Jye

IPC: B01J19/00

CPC classification number: C07H21/00 ,  B01J19/0046 ,  B01J2219/00353 ,  B01J2219/00391 ,  B01J2219/00689 ,  B01J2219/00698 ,  B01J2219/00722 ,  C07K1/045 ,  C40B40/06 ,  C40B60/14 ,  G01F1/708 ,  G01F1/7086 ,  Y10T436/11

Abstract: The present invention makes use of a single detector to monitor several process functions, e.g. reaction efficiency, reagent flow rates, the presence of empty reagent reservoirs, the absence of a chemical reactor column in the system, blockage of flow system, etc., at a single location in the flow system. In accordance with the detection scheme of the present invention, only one detector is required to accomplish the same, if not more, functions as many detectors in the prior art instruments. In the described embodiment, an optical detector is positioned downstream of a chemical reaction chamber. This detector monitors the effluent from the reaction chamber to monitor the reaction efficiency. It also monitors the system functions upstream of the reaction chamber. System flow rate is monitored by detecting the presence of a gas bubble which has been introduced into the system at a known instance. Depletion of reagents in the reservoirs can be detected by monitoring the absence of the reagents at the detector at times when the reagents are expected. The flow system may be periodically diagnosed to check for flow blockage or missing flow component, by monitoring the flow past the detector which corresponds to a predetermined succession of reagents introduced into the system. Any deviation from a predictable succession of changes in the flow monitored by the detector indicates possible blockage of the flow delivery system or missing flow components.

Abstract translation: 本发明利用单个检测器来监视多个过程功能，例如， 反应效率，试剂流量，空试剂储存器的存在，系统中没有化学反应器塔，堵塞流动系统等，在流动系统的单个位置。 根据本发明的检测方案，仅需要一个检测器来实现与现有技术的仪器相同的（如果不是更多的）功能的检测器。 在所描述的实施例中，光学检测器位于化学反应室的下游。 该检测器监测来自反应室的流出物以监测反应效率。 它还监控反应室上游的系统功能。 通过检测在已知情况下已经引入到系统中的气泡的存在来监测系统流量。 可以通过在预期的时候监测检测器处的​​试剂的不存在来检测储存器中试剂的消耗。 可以通过监测通过检测器的流量来对应于引入到系统中的预定的一系列试剂，周期性地诊断流量系统来检查流体阻塞或缺少流动成分。 由检测器监测的流量的可预测的连续变化的任何偏差表明流量输送系统或缺少流动分量的可能的阻塞。

公开(公告)号：WO1993025899A1

公开(公告)日：1993-12-23

申请号：PCT/US1993005809

申请日：1993-06-17

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  GUTTMAN, Andras

IPC: G01N27/447

CPC classification number: G01N27/44773

Abstract: The present invention is directed to a technique that provides enhanced separation resolution in electrophoresis. This technique involves the use of a time-varying field strength, which may be progressively increasing or decreasing, constant or otherwise, as a function of time. The shape of the field strength with respect to time may be continuous or stepwise over time, monotonic or otherwise. Because the mobility of different size species is a function of the applied electric field, the use of a nonuniform (time varying) electric field increases the resolving power of electrophoresis. This technique has been found to provide enhanced resolution of double-stranded DNA molecules in capillary polyacrylamide gel electrophoresis. It has been found that enhanced separation of DNA restriction fragments of 1 to more than 1000 base pairs in size has been achieved employing the technique of the present invention.

公开(公告)号：WO1989004216A1

公开(公告)日：1989-05-18

申请号：PCT/US1988003681

申请日：1988-10-20

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  PENHASI, Harry, Aron

IPC: B04B09/08

CPC classification number: B04B9/08

Abstract: The present invention provides a mechanism for bolting a rotor to a hub without introducing excessive radial stress around the bolt holes when the rotor is operating at high speed. The rotor (1) has a slot cut (35) through its center to eliminate radial stress perpendicular to the slot and thereby avoid the problems of stress cracking of the otherwise weak section. The mounting bolts (501) are necked down and not threaded at the surface of the hub. The bolts are threaded into the hub (501A) at a substantial distance from the top surface of the hub (503) so that they can flex and so that the center of the rotor (1) can move out relative to the hub (32). The hub rotor interface (503) is lubricated to facilitate radial motion of the center of the rotor.

公开(公告)号：WO1989003041A2

公开(公告)日：1989-04-06

申请号：PCT/US1988003368

申请日：1988-09-30

Applicant: BECKMAN INSTRUMENTS, INC.

Inventor： BECKMAN INSTRUMENTS, INC. ,  OH, Chan, S. ,  STERNBERG, James, C.

IPC: G01N33/531

CPC classification number: G01N33/54353 ,  G01N33/531 ,  G01N33/532 ,  G01N33/539 ,  G01N33/54313 ,  G01N33/94 ,  Y10S435/964 ,  Y10S436/819 ,  Y10S530/81 ,  Y10S530/812 ,  Y10S530/816

Abstract: A tridentate conjugate has three chemical moieties, or tridentate members, attached through an appropriate spacer moiety. At least two of the tridentate members are relatively small molecules, usually less than about 7,000 Daltons in size. The particular appropriate spacer moiety selected for a tridentate imparts certain steric properties to the tridentate conjugate. In one embodiment, the binding of a macromolecular specific binding partner to one of the tridentate members sterically inhibits the binding of a different macromolecule to another tridentate member. In another embodiment, the binding of a first tridentate member to a macromolecule restricts the subsequent binding of a second tridentate member to a proximate location on the same macromolecule.

Abstract translation: 具有三个化学部分或三齿元素的三齿共轭物通过适当的间隔半部连接。 至少两个三齿元素是相对小的分子，其大小通常小于7000道尔顿。 为三齿选择适当的半粒径赋予三齿偶联物一定的空间性质。 在一个实施方案中，将特定大分子结合配偶体与三齿元件之一结合，从空间上防止不同大分子与另一三齿元件的结合。 在另一个实施方案中，第一三齿元素与大分子的结合限制了第二三齿元素随后与同一分子上的附近位置的结合。