公开(公告)号：WO2019200307A1

公开(公告)日：2019-10-17

申请号：PCT/US2019/027306

申请日：2019-04-12

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： BARANOV, Vladimir ,  LOBODA, Alexander ,  SULLIVAN, Michael

IPC: G01N33/50 ,  G01N33/532 ,  G01N33/543 ,  G01N33/544 ,  G01N33/566 ,  G01N33/58 ,  G01N30/72

Abstract: Analyzing samples injected into an inductively coupled plasma source can be improved by one or more of a stabilizing solution mixable with a sample prior to injection and a heated injector. The stabilizing solution can minimize the difference in osmotic pressure between the solution and the cells with a relatively low amount of dissolved solids (e.g., at or below about 0.2%). The stabilizing solution can contain a salt (e.g., ammonium nitrate) present in concentrations of at least 5 mM. The injector can be heated before and/or during injection. In some cases, heat from adjacent parts can be channeled into the injector to improve heating of the injector. An injector heated to sufficient temperatures can minimize solute buildup and can extend the usable time between cleanings. These improvements can be especially useful in elemental analysis, such as inductively coupled plasma mass spectrometry or inductively coupled plasma optical emission spectrometry.

公开(公告)号：WO2016126988A1

公开(公告)日：2016-08-11

申请号：PCT/US2016/016637

申请日：2016-02-04

Applicant: FLUIDIGM CANADA INC.

Inventor： LOBODA, Alexander V. ,  BARANOV, Vladimir

IPC: G01N33/53

CPC classification number: H01J49/105 ,  G01N15/10 ,  G01N33/4833 ,  G01N2015/0065 ,  G01N2015/1006 ,  H01J49/0459 ,  H01J49/0463

Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.

Abstract translation: 本发明涉及使用激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）分析样品的方法和装置。 本发明提供方法和装置，其中个体消融羽流被明显地捕获并转移到ICP，随后通过质谱法分析。

公开(公告)号：WO2016109603A1

公开(公告)日：2016-07-07

申请号：PCT/US2015/067961

申请日：2015-12-29

Applicant: FLUIDIGM CANADA INC.

Inventor： LOBODA, Alexander V. ,  BANDURA, Dmitry R. ,  BARANOV, Vladimir I.

IPC: H01J49/26 ,  B01D59/44

CPC classification number: G01N15/1056 ,  G01N2015/0065 ,  G01N2015/1006 ,  H01J49/0009 ,  H01J49/025 ,  H01J49/04 ,  H01J49/105 ,  H01J49/40

Abstract: An improved mass cytometer to facilitate its use for the analysis of particles which may include a sampler, an ion source which is an inductively coupled plasma (ICP) from an ICP torch, wherein the ICP torch may comprise an injector that at the end of the injector proximal to the plasma has an internal diameter of less than 2 mm and more than 250 um, a mass spectrometer, an autosampler, an ion deflector, a dead-time corrector and a mass assignment corrector.

Abstract translation: 改进的质谱仪便于其用于分析可能包括采样器的离子源，离子源，其来自ICP焊炬的电感耦合等离子体（ICP），其中ICP喷枪可以包括喷射器 等离子体附近的注射器具有小于2mm且大于250μm的内径，质谱仪，自动进样器，离子导流器，死区校正器和质量分配校正器。

公开(公告)号：WO2021026483A1

公开(公告)日：2021-02-11

申请号：PCT/US2020/045470

申请日：2020-08-07

Applicant: FLUIDIGM CANADA INC.

Inventor： LIU, Peng ,  MAJONIS, Daniel ,  BARANOV, Vladimir

IPC: A61K51/04 ,  A61K51/00 ,  A61K51/02

Abstract: A new class of mass-tag polymers is provided, which include enriched metal isotopes such as zirconium and hafnium mass tags. The chemistry of these new mass tags are different from that of lanthanide mass tags, and opens up new mass channels that can be used in mass cytometry. These polymers may be used for mass cytometry, therapeutic delivery of a radioactive isotope, or screening of a therapeutic isotope. Aspects include a kit, method of making, and method of using a polymer, isotopic composition, or both. A kit may include a polymer. The polymer may include pendant groups that chelate an enriched isotope, such as zirconium and/or hafnium. The kit may include an isotopic composition comprising an enriched zirconium or hafnium isotope. Polymers may be conjugated to a biologically active material. Aspects may also include making a kit. Aspects include use of a kit, such as for mass cytometry.

公开(公告)号：WO2020257258A1

公开(公告)日：2020-12-24

申请号：PCT/US2020/038097

申请日：2020-06-17

Applicant: FLUIDIGM CORPORATION ,  FLUIDIGM CANADA INC.

Inventor： CORKUM, Paul ,  LOBODA, Alexander

IPC: G01N27/62 ,  G01N15/10 ,  G01N21/71 ,  H01J49/04 ,  H01J49/26 ,  H01J49/40

Abstract: Embodiments of the present invention relate to replacement of the previous ICP-based ionisation system with a new laser ionisation system, providing improved mass spectrometer-based apparatus and methods for using them to analyse samples, in particular the use of mass spectrometry mass cytometry, imaging mass spectrometry and imaging mass cytometry, for the analysis of biological samples. Accordingly, embodiments of the present invention provide an apparatus, for example a mass cytometer, comprising: 1 ) a sampler; 2) a laser ionisation system to receive material removed from the sample by the sampler, wherein the laser ionisation system comprises an ionisation system conduit and a pulsed laser adapted to ionise sample material passing through or exiting the ionisation system conduit; and 3) a mass spectrometer to receive elemental ions from said ionisation system and to analyse said elemental ions.

公开(公告)号：WO2019246033A1

公开(公告)日：2019-12-26

申请号：PCT/US2019/037644

申请日：2019-06-18

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION ,  CORKUM, Paul

Inventor： CORKUM, Paul ,  LOBODA, Alexander

IPC: H01J49/00 ,  H01J49/40 ,  G01N33/574 ,  G01N33/60 ,  G01N33/68 ,  G02B21/33 ,  G02B21/26 ,  G02B21/02

Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMCTM) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.

公开(公告)号：WO2018026910A1

公开(公告)日：2018-02-08

申请号：PCT/US2017/045087

申请日：2017-08-02

Applicant: FLUIDIGM CANADA INC.

Inventor： LOBODA, Alexander V.

IPC: H01J49/04

CPC classification number: H01J49/0463

Abstract: Improved mass spectrometry-based apparatus and methods of analysis for analysing samples. A laser sampling system can be adapted to emit pulses of laser radiation at two or more wavelengths, wherein a first wavelength targets a first material and a second wavelength targets a second material. Atoms of the first and second materials removed by laser ablation are ionized by an ionisation system to form elemental ions, which can be detected by a mass spectrometer component. Laser ablation can be performed at multiple locations to form a plurality of plumes, which can be individually subjected to ionisation and spectrometry. Ablating at known locations or recording locational information for each location can permit construction of an image of the sample(s) based on detecting labelling atoms.

Abstract translation: 改进的基于质谱仪的设备和用于分析样品的分析方法。 激光采样系统可以适用于发射两个或更多波长的激光辐射脉冲，其中第一波长以第一材料为目标，第二波长以第二材料为目标。 通过激光烧蚀去除的第一和第二材料的原子被离子化系统电离以形成元素离子，这可以被质谱仪部件检测到。 激光烧蚀可以在多个位置进行以形成多个羽流，其可以单独进行电离和光谱测定。 在已知位置消除或记录每个位置的位置信息可以允许基于检测标记原子构建样本图像。

公开(公告)号：WO2018026898A1

公开(公告)日：2018-02-08

申请号：PCT/US2017/045060

申请日：2017-08-02

Applicant: FLUIDIGM CANADA INC.

Inventor： LOBODA, Alexander V.

IPC: H01J49/04

Abstract: The invention relates to methods and devices for analysis of samples using laser ablation imaging mass cytometry and mass spectrometry. The invention provides methods and devices in which individual ablation plumes are distinctively captured and rapidly transferred to the ionization system, followed by analysis by mass spectrometry. A transfer conduit can be used to convey ablation plumes to an ionization system. The transfer conduit can include an asymmetric cone. The transfer conduit can be tapered. A flow sacrificing system can be adapted to divert a part of the sheath flow out a sacrificial outlet while the core of the sheath flow containing ablation plumes enters the ionization system.

Abstract translation: 本发明涉及使用激光消融成像质量细胞计量术和质谱法分析样品的方法和装置。 本发明提供了各种方法和装置，其中单独的消融羽流被明显地捕获并迅速转移到电离系统中，随后通过质谱分析。 传输导管可用于将消融羽流传输至电离系统。 输送管道可以包括不对称锥体。 传输导管可以是锥形的。 流量牺牲系统可以适用于将鞘流的一部分转移出牺牲出口，同时包含消融羽流的鞘流的核心进入电离系统。

公开(公告)号：WO2016105418A1

公开(公告)日：2016-06-30

申请号：PCT/US2014/072377

申请日：2014-12-24

Applicant: FLUIDIGM CANADA INC.

Inventor： BARANOV, Vladimir

IPC: G01N15/10

CPC classification number: G01N15/1031 ,  G01N15/1427 ,  G01N15/1429 ,  G01N15/1459 ,  G01N27/622 ,  G01N2015/1006 ,  G01N2015/1043 ,  G01N2015/1477

Abstract: This disclosure is directed to systems and methods that enable optical-based cytometric techniques and mass cytometric techniques to be used in the investigation of the same set of particles. The systems and methods include an optical analysis system that individually receives one or more particles of a sample of particles. The optical analysis system generates optical particle data for each optically detectable particle of the sample that passes through the optical analysis system. A mass cytometry system also receives each particle that passes through the optical analysis system and generates mass particle data for each detectable particle that enters the mass cytometry system. The methods and systems correlate the optical particle data and the mass particle data associated with the same particles.

Abstract translation: 本公开涉及使得基于光学的细胞计算技术和质谱技术可用于研究相同组的颗粒的系统和方法。 该系统和方法包括单独接收一个或多个颗粒样品颗粒的光学分析系统。 光学分析系统为通过光学分析系统的样品的每个可光学检测的颗粒产生光学粒子数据。 质量细胞计量系统还接收通过光学分析系统的每个颗粒，并为进入质谱仪系统的每个可检测的颗粒产生质量粒子数据。 方法和系统将光学粒子数据和与相同粒子相关联的质量粒子数据相关联。

公开(公告)号：CA3193031A1

公开(公告)日：2022-03-24

申请号：CA3193031

申请日：2021-09-09

Applicant: FLUIDIGM CANADA INC

Inventor： LOBODA ALEXANDER ,  JONG RAYMOND ,  SULLIVAN MICHAEL ,  VOROBIEV SERGUEI ,  ROTENBERG ROBERT ,  STRATULATIV EMIL ,  VORONOV MAXIM ,  ARMSTRONG MARK

IPC: G01N21/73 ,  H05H1/26 ,  H05H1/30 ,  H05H1/34

Abstract: Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.