게놈 수준에서의 클루이베로마이세스 마르시아누스의 대사 네트워크 모델 및 이를 이용한 3HP 생산에 대한 대사특성분석 방법
    123.
    发明公开
    게놈 수준에서의 클루이베로마이세스 마르시아누스의 대사 네트워크 모델 및 이를 이용한 3HP 생산에 대한 대사특성분석 방법 无效
    基因重组代谢网络模型重建KLUYVEROMYCES MARXIANUS和策略,用于在KLUYVEROMYCES MARXIANUS中制备3-羟基丙酸生产的非生物途径

    公开(公告)号:KR1020140015998A

    公开(公告)日:2014-02-07

    申请号:KR1020120082816

    申请日:2012-07-27

    CPC classification number: G06F19/12 C12P7/42

    Abstract: An embodiment relates to a metabolic network model for analyzing metabolic characteristics of Kluyveromyces marxianus microbes for producing 3-hydroxypropionate (3HP) and to metabolic characteristic analyzation of the Kluyveromyces marxianus using the same. More specifically, the embodiment relates to construction of a metabolic network model of Kluyveromyces marxianus using gene-protein-biochemical response relationship, to analyzation of metabolic characteristic using the model such as metabolic engineering, and to a method for predicting a novel metabolic pathway which enhances productivity of 3HP using simulation based on the metabolic engineering. The method of the embodiment efficiently predicts productivity and cell growth speed of microbes, saves time and costs in order to optimize the novel path, and provides variant microorganisms which can provide specific metabolites.

    Abstract translation: 一个实施方案涉及用于分析用于产生3-羟基丙酸酯(3HP)的马克斯克鲁维酵母微生物代谢特征的代谢网络模型和使用其的马克斯克鲁维酵母的代谢特征分析。 更具体地,本实施例涉及使用基因 - 蛋白质 - 生物化学反应关系构建马克斯克鲁维酵母的代谢网络模型,使用代谢工程等模型分析代谢特征,以及用于预测增强的新型代谢途径的方法 使用基于代谢工程的模拟,3HP的生产率。 本实施例的方法有效地预测微生物的生产力和细胞生长速度,节省时间和成本,以便优化新的途径,并提供可提供特异性代谢物的变体微生物。

    3,6-L-AHG 전환효소의 활성측정용 조성물, 이를 이용한 3,6-L-AHG 전환효소의 활성 측정방법 및 3,6-L-AHG의 정량분석용 조성물
    125.
    发明公开
    3,6-L-AHG 전환효소의 활성측정용 조성물, 이를 이용한 3,6-L-AHG 전환효소의 활성 측정방법 및 3,6-L-AHG의 정량분석용 조성물 有权
    用于测量3,6-L-AHG转移酶活性的组合物,使用其测量3,6- L-AHG转移活性的方法和用于3,6- L-AHG定量分析的组合物

    公开(公告)号:KR1020110136441A

    公开(公告)日:2011-12-21

    申请号:KR1020100056459

    申请日:2010-06-15

    CPC classification number: C12Q1/48 C12N9/1051 G01N2333/91102

    Abstract: PURPOSE: A composition for measuring 3,6-anhydro-L-galactose transferase activity is provided to be used in an industry. CONSTITUTION: A composition for measuring 3,6-L-AHG transferase contains NADP+ as a coenzyme, 3,6-anhydro-L-galactose(3,6-L-AHG), and buffer solution. A method for measuring the activity of 3,6-L-AHG transferase comprises: a step of reacting random enzyme extract liquid and a composition for measuring the activity of 3,6-L-AHG tranferase; a step of measuring reduction into NADPH by the reaction. A composition for quantitation of 3,6-L-AHG contains an active fraction containing 3,6-L-AHG tranferase, NADP+ as a coenzyme, and buffer solution.

    Abstract translation: 目的:用于测量3,6-脱水-L-半乳糖转移酶活性的组合物用于工业中。 构成:用于测量3,6-L-AHG转移酶的组合物含有作为辅酶的NADP +,3,6-脱水-L-半乳糖(3,6-L-AHG)和缓冲溶液。 测定3,6-L-AHG转移酶的活性的方法包括:使随机酶提取液与用于测定3,6-L-AHG转移酶活性的组合物反应的步骤; 通过反应测量还原成NADPH的步骤。 用于定量3,6-L-AHG的组合物含有含有3,6-L-AHG转移酶,NADP +作为辅酶的活性级分和缓冲溶液。

    갈조류를 이용한 바이오연료의 제조방법
    128.
    发明公开
    갈조류를 이용한 바이오연료의 제조방법 无效
    使用棕色阿尔法制备生物素的方法

    公开(公告)号:KR1020110010346A

    公开(公告)日:2011-02-01

    申请号:KR1020090067863

    申请日:2009-07-24

    Abstract: PURPOSE: A method for preparing bio-fuel using brown algae is provided to effectively saccharify brown algae and use in producing industrial bio-fuel. CONSTITUTION: A bio-fuel is prepared by adding hydrolyze catalyst of Bacterium Antarctica, culture liquid of Bacterium Antarctica, supernatant of the culture liquid, or pulverized liquid of the culture liquid to brown algae through sacchificiation. The brown algae is laminaria japonica, gulfweed, ecklonia cava, rhei Rhizoma, or sargassum thunbergii. The Bacterium Antarctica contains 16S rRNA having 95% of homology with a sequence corresponding to Bacterium antarctica AL-1(deposit number KCTC 11531 BP). A base sequence of 16S rRNA has sequence number 1.

    Abstract translation: 目的:提供使用棕色藻类制备生物燃料的方法,以有效地糖化褐藻,并用于生产工业生物燃料。 构成:通过将南极洲的水解催化剂,南极洲细菌的培养液,培养液的上清液或培养液的粉碎液通过糖化将棕色藻类加入生物燃料中制备生物燃料。 棕色藻类是lamin粳,草甘膦,ecklonia cava,rhei Rhizoma或sargassum thunbergii。 南极洲细菌含有与对应于南极洲AL-1(保藏号KCTC 11531BP)的序列具有95%同源性的16S rRNA。 16S rRNA的碱基序列具有序列号1。

    리그노셀룰로오스계 바이오매스의 전처리 방법
    129.
    发明公开
    리그노셀룰로오스계 바이오매스의 전처리 방법 无效
    基于木质素的生物质的预处理方法

    公开(公告)号:KR1020100119018A

    公开(公告)日:2010-11-09

    申请号:KR1020090037915

    申请日:2009-04-30

    Abstract: PURPOSE: A pretreatment method of a lignocellulose-based biomass is provided to obtain cellulose with high purity and hemicelluloses with the high yield, by extracting lignin first. CONSTITUTION: A pretreatment method of a lignocellulose-based biomass comprises the following steps: adding a solvent dissolving lignin to the lignocellulose-based biomass containing the lignin, hemicelluloses, and cellulose, to extract the lignin from the biomass(S1,S2); and adding an ionic solution to the biomass without the lignin, to extract the hemicelluloses and the cellulose(S3). The solvent dissolving the lignin is an alkaline solvent.

    Abstract translation: 目的:提供木质纤维素基生物质的预处理方法,首先通过提取木质素,以高产率获得高纯度的纤维素和半纤维素。 构成:木质纤维素基生物质的预处理方法包括以下步骤:向含木质素,半纤维素和纤维素的木质纤维素基生物质中加入溶解木质素的溶剂,从生物质提取木质素(S1,S2); 并将离子溶液加入到没有木质素的生物质中,以提取半纤维素和纤维素(S3)。 溶解木质素的溶剂是碱性溶剂。

    전기화학적 방법에 의하여 시료에 포함된 전해질 검출이 가능한 디스크형 미세유동장치
    130.
    发明公开
    전기화학적 방법에 의하여 시료에 포함된 전해질 검출이 가능한 디스크형 미세유동장치 无效
    通过电化学方法检测样品中含有电解质的DISC型微流体装置

    公开(公告)号:KR1020100083029A

    公开(公告)日:2010-07-21

    申请号:KR1020090002383

    申请日:2009-01-12

    Abstract: PURPOSE: A disk-shape microflow apparatus capable of detecting electrolyte included in a sample by an electrochemical method is provided to effectively perform an electrochemical detection of electrolyte by using one or more electrochemical analysis unit which is formed on a rotary disk-shape microflow apparatus. CONSTITUTION: A disk-shape microflow apparatus capable of detecting electrolyte included in a sample by an electrochemical method comprises a sample chamber(211) which accepts the sample, a detection chamber(220) which is offered the sample from the sample chamber, and an ion sensor(250) which electrochemically detects electrolyte among the sample with being arranged in the detection chamber and includes an indicator electrode(252) wherein an ion selection layer(253) is prepared in a reference electrode and an end part.

    Abstract translation: 目的:提供一种能够通过电化学方法检测样品中的电解质的盘状微流量装置,通过使用形成在旋转盘状微流量装置上的一个或多个电化学分析单元来有效地进行电解质的电化学检测。 构成:能够通过电化学方法检测样品中的电解质的盘状微流装置包括:样品室(211),其接收样品;检测室(220),其从样品室提供样品;以及 离子传感器(250),其电化学地检测样品中的电解质,并布置在检测室中,并且包括在参考电极和端部中制备离子选择层(253)的指示电极(252)。

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