公开(公告)号：DE102005063191A1

公开(公告)日：2007-07-05

申请号：DE102005063191

申请日：2005-12-30

Applicant: BASF AG

Inventor： SCHAEDLER ANDREAS ,  FRIEDRICH THOMAS ,  STUERMER RAINER ,  RINCK-JAHNKE SABINE

IPC: C12P7/24 ,  C12N9/02 ,  C12P7/04

Abstract: Preparation of an optically active saturated aldehyde or alcohol (II) comprises reduction of alpha , beta unsaturated aldehyde (I) with a polypeptide sequence (SEQ ID NO: 1 or 2), or a polypeptide sequence exhibiting at least 80% sequence identity of (SEQ ID NO: 1 or 2), in the presence of an enoate reductase. Preparation of an optically active saturated aldehyde or alcohol of formula (II) comprises reduction of alpha , beta unsaturated aldehyde (I) of formula (R 3>-C(R 2>)=C(R 1>)-CH(=O)) with a polypeptide sequence (SEQ ID NO: 1 or 2) (not defined), or a polypeptide sequence exhibiting at least 80% sequence identity of (SEQ ID NO: 1 or 2), in the presence of a enoate reductase. R 1>, R 2>H or 1-4C-alkyl; and R 3>H or 1-6C alkyl or alkenyl. [Image].

公开(公告)号：PT1123386E

公开(公告)日：2007-03-30

申请号：PT99952558

申请日：1999-10-13

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  FRIEDRICH THOMAS ,  RESS-LOESCHKE MARION ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C07H21/00 ,  C12N15/09 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

公开(公告)号：AU2001284047B2

公开(公告)日：2007-03-29

申请号：AU2001284047

申请日：2001-08-30

Applicant: BASF AG

Inventor： FRIEDRICH THOMAS ,  STURMER RAINER ,  LADNER WOLFGANG ,  HAUER BERNHARD ,  NUBLING CHRISTOPH

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/16 ,  C12N9/18 ,  C12N15/55 ,  C12P7/62 ,  C12P41/00

Abstract: The invention relates to novel proteins from Pseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefore, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.

公开(公告)号：DE102005044736A1

公开(公告)日：2007-03-22

申请号：DE102005044736

申请日：2005-09-19

Applicant: BASF AG

Inventor： BREUER MICHAEL ,  FRIEDRICH THOMAS ,  KESELER MARIA

IPC: C12N9/04 ,  C12N15/53 ,  C12N15/63 ,  C12P7/02 ,  C12P7/22 ,  C12P17/00

Abstract: The present invention relates to proteins having an enzymatic activity of reducing substituted alkanones such as 3-methylamino-1-(2-thienyl)-propan-1-one. The invention furthermore relates to nucleic acids coding for said proteins, nucleic acid constructs, vectors, genetically modified microorganisms and to methods for preparing optically active substituted alkanols, such as, for example, (S)-3-methylamino-1-(2-thienyl)-(S)-propanol.

公开(公告)号：DE59914102D1

公开(公告)日：2007-02-08

申请号：DE59914102

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：DE102004022686A1

公开(公告)日：2005-11-24

申请号：DE102004022686

申请日：2004-05-05

Applicant: BASF AG

Inventor： STUERMER RAINER ,  KESELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  BREUER MICHAEL ,  SCHROEDER HARTWIG

IPC: C07D333/16 ,  C12N9/04 ,  C12P20060101 ,  C12P7/02 ,  C12P13/00 ,  C12P17/00

Abstract: The invention relates to a method for producing the optically active alkanols of formula (I), wherein n is an integer of from 0 to 5; Cyc represents an optionally substituted, mononuclear or polynuclear, saturated or unsaturated, carbocylic or heterocyclic ring, and R1 represents halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, wherein R2, R3 and R4 independently represent H or a lower alkyl or lower alkoxy group and X− represents a counterion. According to the invention, an enzyme (E) selected from the groups of dehydrogenases, aldehyde reductases and carbonyl reductases is incubated in a medium containing the alkanone of formula (II), wherein n, Cyc and R1 are defined as above, in the presence of reduction equivalents. The compound of formula (II) is enzymatically reduced to the compound of formula (I) and the reduction equivalents consumed during reaction are regenerated by reacting a sacrificial alcohol to the corresponding sacrificial ketone using enzyme (E) and at least partially removing the sacrificial ketone from the reaction medium, and then isolating the product (I) so produced.

公开(公告)号：NO20031213D0

公开(公告)日：2003-03-17

申请号：NO20031213

申请日：2003-03-17

Applicant: BASF AG

Inventor： FRIEDRICH THOMAS

IPC: C12P9/00 ,  C12P

公开(公告)号：DE10117730A1

公开(公告)日：2002-10-10

申请号：DE10117730

申请日：2001-04-09

Applicant: BASF AG

Inventor： FRIEDRICH THOMAS ,  ZIMMERMANN NORBERT ,  STUERMER RAINER

IPC: C07D211/20 ,  C07D265/30 ,  C07D279/12 ,  C12N9/06 ,  C12P7/04 ,  C12P7/18 ,  C12P7/22 ,  C12P13/00 ,  C12P13/24 ,  C12P17/10 ,  C12P17/12 ,  C12P17/14

Abstract: One-pot reaction of amines (I) to cyclic amines (II) or alcohols (III) by reaction in presence of lysine oxidase (LO) and reduction. One-pot reaction of amines (I) to cyclic amines (II) or alcohols (III) by reaction in presence of lysine oxidase (LO) and reduction. The process comprises: (1) oxidation of at least one C-NH2 group to carbonyl; (2) optionally reaction of second amino (where A = A1) with the carbonyl group to form an enamine or imine; and (3) reduction of the carbonyl to convert it to hydroxymethyl (or of the product of step (2) to form (II)). A = NH2-CHR -R - (A1), NH2CH2-R - or R -; B = HOCH2-R3- or R -; R = NR or 2-6C linear divalent hydrocarbyl (optionally substituted by carboxy, COOR , OH, mercapto and/or NR 2 and/or including non-adjacent O, S and/or N atoms); R = H or linear 1-6C alkyl; R = linear or branched 1-6C alkyl or 6-10C aryl; R = H, carboxy, COOR or cyano; R = bivalent 0-1 or 5-18C hydrocarbyl (optionally substituted by 1-6C linear or branched alkyl, 6-10C aryl, 4-10C heteroaryl, and/or COOR and/or including non-adjacent O, S and/or N atoms); R = linear 1-20C hydrocarbyl, optionally substituted with functional groups such as carboxy or COOR and/or including non-adjacent O, S and/or N atoms.

公开(公告)号：BR0015114A

公开(公告)日：2002-07-16

申请号：BR0015114

申请日：2000-10-20

Applicant: BASF AG

Inventor： KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  MATTES RALF

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/16 ,  C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00 ,  C12R1/19

Abstract: The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.

公开(公告)号：CA2422600A1

公开(公告)日：2002-03-21

申请号：CA2422600

申请日：2001-09-17

Applicant: BASF AG

Inventor： FRIEDRICH THOMAS

IPC: C12P9/00

Abstract: The invention relates to a method for the polycondensation of organic silico n compounds at pH values between 6 and 8 in the presence of a lipase, said lipase being optionally immobilised on a carrier consisting of polymer materials. Suitable polycondensable organic silicon compounds are (RO)(R1O)(R2O)(R3O)Si, (RO)(R1O)(R2O)(R3O)SiR3, (RO)(R1O)Si(R2)(R3) and (RO)SiR1R2R3 with R, R1, R2 and R3 independently representing C1- to C10- alkyl, C3- to C10-cycloalkyl, C4- to C20-alkylcycloalkyl, aryl, C6- to C16- alkylaryl, the alkyl groups being linear or branched. It is advantageous tha t the lipase can be obtained relatively easily by means of large-scale fermentation processes. Lipases from Pseudomona species are preferable.