公开(公告)号：CA2395694C

公开(公告)日：2006-11-21

申请号：CA2395694

申请日：2000-12-22

Applicant: ADVION BIOSCIENCES INC

Inventor： CORSO THOMAS N ,  PROSSER SIMON J ,  SCHULTZ GARY A

IPC: G01N27/62 ,  H01J49/04 ,  B05B5/08 ,  G01N21/33 ,  G01N21/53 ,  G01N21/64 ,  G01N27/00 ,  G01N27/447 ,  G01N30/88 ,  G01N37/00 ,  H01J49/10 ,  H01J49/26

Abstract: A microchip-based electrospray device, system, and method of fabrication thereof are disclosed. The electrospray device (250) includes a substrate (200) defining a channel (224) between an entrance orifice on an injection surface and an exit orifice on an ejection surface, a nozzle (232) defined by a portion recessed from the ejection surface surrounding the exit orifice, and an electric field generating source for application of an electric potential to the substrate to optimize and generate an electrospray (262). A method and system are disclosed to generate multiple electrospray plumes from a single fluid stream that provides an ion intensity as measured by a mass spectrometer that is approximately proportional to the number of electrospray plumes formed for analytes contained within the fluid. A plurality of electrospray nozzle devices (232) can be used in the form of an array of miniaturized nozzles for the purpose of generating multiple electrospray plumes (262) from multiple nozzles (232) for the same fluid stream. This invention dramatically increases the sensitivity of microchip electrospray devices (250) compared to prior disclosed systems and methods.

公开(公告)号：AU2002240143A1

公开(公告)日：2002-08-06

申请号：AU2002240143

申请日：2002-01-28

Applicant: ADVION BIOSCIENCES INC

Inventor： SCHULTZ GARY A ,  CORSO THOMAS N ,  PROSSER SIMON J ,  RULE GEOFFREY S

IPC: G01N27/62 ,  G01N1/00 ,  G01N35/00 ,  G01N35/04 ,  G01N35/10 ,  H01J49/04 ,  B01D59/44 ,  H01J49/00

Abstract: A robotic autosampler provides for automated manipulation of microfluidic chips having multiple electrospray devices and/or sample inlets for interface to a mass spectrometer or other detection device. The autosampler also provides for connection of control voltages to the electrospray device to facilitate enablement, control and steering of charged droplets and ions. The autosampler further provides a method of fluid delivery that may be disposable or reusable. The delivery device may contain materials for component separation or sample purification. The delivery device may contain preloaded sample or the sample may be loaded by the autosampler. A method for automated manipulation of multiple electrosprays in communication with a detector, includes: providing a robot autosampler having an electrospray chip; electrospraying at least one analyte from at least one electrospray device on the electrospray chip; and manipulating the electrospray chip in communication with a detector in a manner to detect analyte from the electrospray.

公开(公告)号：CA2363414C

公开(公告)日：2008-02-05

申请号：CA2363414

申请日：2000-02-29

Applicant: ADVION BIOSCIENCES INC

Inventor： CORSO THOMAS N ,  SCHULTZ GARY A ,  GALVIN GREGORY J ,  DAVIS TIMOTHY J ,  MOON JAMES E

IPC: B05B5/025 ,  B81C1/00 ,  G01N27/26 ,  G01N27/447 ,  G01N30/60 ,  G01N30/72 ,  H01J49/04 ,  H01J49/10

Abstract: A droplet/electrospray device and a liquid chromatography-electrospray system are disclosed. The droplet/electrospray device (100) comprises a substrate (102) defining a channel (104) between an entrance orifice (106) on an injection surface (108) and an exit orifice on an ejection surface (112), a nozzle (110) defined by a portion recessed from the ejection surface (112) surrounding the exit orifice, and an electrode (122) for application of an electric potential to the substrate (102) to optimize and generate droplets or an electrospray (62). A plurality of these electrospray devices (100) can be used in the fonn of an array of miniaturized nozzles. The liquid chromatography-electrospray device (160) comprises a separation substrate (162) defining an introduction channel (164) between an entrance orifice and a reservoir (166) and a separation channel (168) between the reservoir (166) and an exit orifice (170), the separation channel (168) being populated with separation posts (174) perpendicular to the fluid flow.

公开(公告)号：HK1066863A1

公开(公告)日：2005-04-01

申请号：HK04109844

申请日：2004-12-13

Applicant: ADVION BIOSCIENCES INC ,  KIONIX INC

Inventor： MOON JAMES E ,  SCHULTZ GARY A ,  CORSO THOMAS N ,  DAVIS TIMOTHY J ,  GALVIN GREGORY J ,  LOWES STEPHEN

IPC: G01N20060101 ,  B01D20060101 ,  G01N27/62 ,  B01D15/08 ,  G01N30/60 ,  G01N30/72 ,  G01N37/00 ,  H01J20060101 ,  H01J49/04

Abstract: An electrospray device, a liquid chromatography device and an electrospray-liquid chromatography system are disclosed. The electrospray device comprises a substrate defining a channel between an entrance orifice on an injection surface and an exit orifice on an ejection surface, a nozzle defined by a portion recessed from the ejection surface surrounding the exit orifice, and an electrode for application of an electric potential to the substrate to optimize and generate an electrospray; and, optionally, additional electrode(s) to further modify the electrospray. The liquid chromatography device comprises a separation substrate defining an introduction channel between an entrance orifice and a reservoir and a separation channel between the reservoir and an exit orifice, the separation channel being populated with separation posts perpendicular to the fluid flow; a cover substrate bonded to the separation substrate to enclose the reservoir and the separation channel adjacent the cover substrate; and, optionally, electrode(s) for application of a electric potential to the fluid. The exit orifice of the liquid chromatography device may be homogeneously interfaced with the entrance orifice of the electrospray device to form an integrated single system. An array of multiple systems may be fabricated in a single monolithic chip for rapid sequential fluid processing and generation of electrospray for subsequent analysis, such as by positioning the exit orifices of the electrospray devices near the sampling orifice of a mass spectrometer.

公开(公告)号：AU6977301A

公开(公告)日：2001-12-17

申请号：AU6977301

申请日：2001-06-11

Applicant: ADVION BIOSCIENCES INC

Inventor： HUANG XIAN

IPC: G01N30/26 ,  B01D15/08 ,  B01J20/26 ,  B01J20/281 ,  B01J20/285 ,  B01J20/287 ,  B01J20/32 ,  C08J9/36 ,  G01N30/34 ,  G01N30/72 ,  G01N30/88

公开(公告)号：AU3506100A

公开(公告)日：2000-09-21

申请号：AU3506100

申请日：2000-02-29

Applicant: ADVION BIOSCIENCES INC

Inventor： SCHULTZ GARY A ,  CORSO THOMAS N

IPC: G01N30/60 ,  G01N30/72 ,  H01J49/04 ,  G01N27/26 ,  H01J49/10

Abstract: An electrospray device comprises a capillary tube (52) having a passage for conducting fluids through the capillary tube and connecting an entrance orifice and an exit orifice (56). First and the second electrodes are positioned to define an electric field surrounding the exit orifice (56). The first electrode (70) circumscribes the capillary tube proximate the exit orifice, and the second electrode imparts a second potential to the fluid. A mass spectrometry device may be provided to receive fluid droplets/sprays of fluid (60,62) from the exit orifice (56).

公开(公告)号：WO2007047542A3

公开(公告)日：2009-06-04

申请号：PCT/US2006040285

申请日：2006-10-13

Applicant: ADVION BIOSCIENCES INC

Inventor： LI JIE ,  CORSO THOMAS N

IPC: C25B9/00 ,  B01D59/44 ,  C23C14/35 ,  C23C16/00

CPC classification number: H01J49/167

Abstract: A method of preventing analyte electrolysis in use with electrospray ionization, electrophoresis, electro osmosis, electrodialysis and any apparatuses involving contact of liquids and electrodes is disclosed. The method for preventing analyte alteration by electrolysis reactions at electrode surfaces of an electrochemical system and in an electrochemical process includes coating the electrode surface using electrically insulating material including but not limited to polymers, plastics, and organic compounds by coating methods including but not limited to liquid spraying, spinning, molding, Sol Gel, dipping, physical vapor deposition and chemical vapor deposition at various ambient and substrate temperatures.

Abstract translation: 公开了一种使用电喷雾离子化，电泳，电渗透，电渗析以及涉及液体和电极接触的任何装置来防止分析物电解的方法。 用于防止通过电化学反应在电化学系统的电极表面和电化学过程中的分解物改变的方法包括使用包括但不限于聚合物，塑料和有机化合物的电绝缘材料涂覆电极表面，所述涂覆方法包括但不限于 液体喷涂，纺丝，成型，溶胶凝胶，浸渍，物理气相沉积和化学气相沉积在各种环境和基板温度。

公开(公告)号：WO2003036304A1

公开(公告)日：2003-05-01

申请号：PCT/US2002/034495

申请日：2002-10-28

Applicant: ADVION BIOSCIENCES, INC.

Inventor： SCHULTZ, Gary, A. ,  SHELDON, Gary, S. ,  JUNG, David, R. ,  ALPHA, Christopher, G. ,  RULE, Geoffrey, S. ,  HUANG, Xian ,  PROSSER, Simon, J.

IPC: G01N35/02

CPC classification number: G01N30/6034 ,  B01L3/0275 ,  B01L9/06 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/142 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/0832 ,  G01N30/24 ,  G01N30/463 ,  G01N30/466 ,  G01N30/468 ,  G01N30/6004 ,  G01N30/6039 ,  G01N30/6043 ,  G01N30/6047 ,  G01N30/6052 ,  G01N30/6091 ,  G01N30/7233 ,  G01N2030/8881 ,  G01N2035/00237 ,  G01N2035/00326 ,  Y10T436/25 ,  Y10T436/255 ,  Y10T436/2575

Abstract: A disposable tube (80) includes an inlet end (81) and an outlet end (82), wherein the inlet end of a first tube is self-locking, self-aligning, self-mating, self-sealing and adapted to detachably engage an outlet end of a second tube. The tube may be filled with separation material. The tubes may be used for micro fluidic separation and fluid transfer. Also included is a tube array having a plurality of tube holders adjacent to one another. Each tube holder has a passageway configured to receive a tube. Each passageway constrains the movement of a tube in the array: allowing free movement of the tube along the tube axis, while allowing limited sideways movement of the tube, so that the tube is held in alignment with a corresponding input port of, for example, a sample transfer device.

Abstract translation: 一次性管（80）包括入口端（81）和出口端（82），其中第一管的入口端是自锁的，自对准的，自配合的，自密封的并适于可拆卸地接合 第二管的出口端。 管可以填充分离材料。 管可用于微流体分离和流体转移。 还包括具有彼此相邻的多个管保持器的管阵列。 每个管保持器具有构造成接收管的通道。 每个通道限制管阵列中的管的移动：允许管沿着管轴自由移动，同时允许管的有限侧向移动，使得管保持与例如相应的输入端口对准， 样品转印装置。

公开(公告)号：WO2003025992A1

公开(公告)日：2003-03-27

申请号：PCT/US2002/029505

申请日：2002-09-17

Applicant: ADVION BIOSCIENCES, INC.

Inventor： LI, Jie ,  CORSO, Thomas, N. ,  SCHULTZ, Gary, A.

IPC: H01L21/306

CPC classification number: H01B7/2825 ,  G01N27/44704 ,  G01N30/7266 ,  H01B3/10 ,  H01B3/12 ,  H01L21/3143 ,  H01L21/3145 ,  H01L21/3146

Abstract: A dielectric film is provided which includes a base layer and a capping layer, preferably silicon oxynitride, wherein the film is an effective moisture and ion barrier when disposed between a conductive substrate and a liquid having an electrical potential different than the electrical potential of the substrate.

Abstract translation: 提供了一种电介质膜，其包括基底层和覆盖层，优选氮氧化硅，其中当设置在导电衬底和具有不同于衬底的电位的电位的电位的液体之间时，膜是有效的水分和离子屏障 。

公开(公告)号：WO2003025983A1

公开(公告)日：2003-03-27

申请号：PCT/US2002/029508

申请日：2002-09-17

Applicant: ADVION BIOSCIENCES, INC.

Inventor： SHELDON, Gary, S.

IPC: H01L21/00

CPC classification number: B41J2/1632 ,  B41J2/162 ,  B41J2/1628 ,  B41J2/1631 ,  B41J2/1635 ,  B41J2/1642

Abstract: A microchip-based electrospray device and method of fabrication thereof are disclosed. The electrospray device includes a substrate (300) defining a channel (336) between an entrance orifice on an injection surface and an exit orifice on an ejection surface, a nozzle defined by a portion recessed from the ejection surface surrounding the exit orifice, and an electric field generating source for application of an electric potential to the substrate to optimize and generate an electrospray. The method includes providing a nozzle and annulus (338) pattern to the polished side of a wafer. The nozzle channel is etched and the back side of the wafer lapped or ground until the nozzle through channel is exposed. The annulus etch may be conducted prior to or following the backgrinding process.

Abstract translation: 公开了一种基于微芯片的电喷雾装置及其制造方法。 电喷射装置包括衬底（300），其限定在注射表面上的入口孔和喷射表面上的出口孔之间的通道（336），由从围绕出口孔的喷射表面凹陷的部分限定的喷嘴，以及 电场产生源，用于向衬底施加电势以优化并产生电喷雾。 该方法包括向晶片的抛光侧提供喷嘴和环形（338）图案。 蚀刻喷嘴通道，并且晶片的背面重叠或研磨，直到喷嘴通过通道暴露。 环形蚀刻可以在背面研磨工艺之前或之后进行。