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公开(公告)号：KR1020130046048A
公开(公告)日：2013-05-07
申请号：KR1020110110371
申请日：2011-10-27
Applicant: 강원대학교산학협력단
CPC classification number: C12N15/8205 , A01H4/001 , A01H4/005 , A01H4/008 , C12N5/0025 , C12N15/8209 , C12N15/8241 , C12N2500/34 , C12N2501/30
Abstract: PURPOSE: A method for producing a transgenic Miscanthus plant is provided to obtain Miscanthus plants with high transformation efficiency using callus. CONSTITUTION: A method for producing a transgenic Miscanthus plant comprises: a step of placing a Miscanthus seed on a callus-inducing medium and inducing Miscanthus callus; a step of co-culturing the induced Miscanthus callus and Agrobacterium tumefaciens containing a foreign gene-inserted plant expression vector; a step of culturing the co-cultured plant cells in a selective medium containing antibiotics; a step of elongating the selected Miscanthus cells in an elongation medium; and a step of inducing rooting of the elongated cells in a medium for inducing rooting.
Abstract translation: 目的：提供一种生产转基因芒草植物的方法，以使用愈伤组织获得高转化效率的芒草。 构成：用于生产转基因芒草植物的方法包括：将芒草种子置于愈伤组织诱导培养基上并诱导芒草的步骤; 共培养含有外源基因插入植物表达载体的诱导的芒草和根癌土壤杆菌的步骤; 在含有抗生素的选择培养基中培养共培养的植物细胞的步骤; 在延伸培养基中拉长所选的芒草细胞的步骤; 以及在用于诱导生根的培养基中诱导细长细胞的生根的步骤。
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公开(公告)号：KR101203536B1
公开(公告)日：2012-11-21
申请号：KR1020100046006
申请日：2010-05-17
Applicant: 강원대학교산학협력단 , 삼성생약 주식회사 농업회사법인
Abstract: 본 발명은 비타민나무(
Hippophae rhamnoides L.)를 이용한 증류식 소주 및 이의 제조방법에 관한 것으로, 더욱 상세하게는 비타민나무를 이용한 증류식 소주는 소주 제조과정 중 비타민나무에 함유된 유효성분이 손실되지 않고, 비타민나무 잎의 향과 맛이 부여되면서 종래의 증류식 소주에 비해 기호도가 향상되므로, 건강을 증진시키고 향미가 뛰어난 증류식 소주의 제조를 위해 유용하게 사용될 수 있다.-
公开(公告)号：KR100835710B1
公开(公告)日：2008-06-09
申请号：KR1020070095740
申请日：2007-09-20
Applicant: 강원대학교산학협력단
Abstract: 본 발명은 케나프 추출물과 이로부터 분리된 신규 캠프페롤 화합물 및 이의 식품으로서의 용도에 관한 것으로서, 더욱 상세하게는 케나프(
Hibiscus
cannabbinus L., Malvaceae) 추출물과 이로부터 분리된 신규 캠프페롤 화합물이 항균 및 항산화 활성을 나타냄을 밝히고, 이를 식품으로 적용하기 위한 새로운 용도에 관한 것이다.
케나프(Hibiscus cannabbinus L., Malvaceae), 캠프페롤 화합물-
公开(公告)号：KR100815439B1
公开(公告)日：2008-03-20
申请号：KR1020070014897
申请日：2007-02-13
Applicant: 강원대학교산학협력단
CPC classification number: A01H4/005 , A01H4/001 , A01H4/008 , C12N5/0025 , C12N2500/34 , C12N2501/30
Abstract: A method for mass-producing an Oplopanax elatus Nakai plantlet is provided to mass-propagate the Oplopanax elatus Nakai effectively from embryogenetic callus derived from an Oplopanax elatus Nakai tissue, thereby preserving endangered Oplopanax elatus Nakai. A method for mass-producing an Oplopanax elatus Nakai plantlet comprises the steps of: (a) explanting a tissue segment of Oplopanax elatus Nakai a cell tissue cultures in an MS culture medium where a plant growth control material including TDZ is added to induce embryogenetic callus; (b) culturing the induced embryogenetic callus in a liquid culture medium or a solid culture medium to induce somatic embryos; (c) culturing the induced somatic embryos in a liquid culture medium or a solid culture medium to propagate them; (d) culturing the propagated somatic embryos in a liquid culture medium or a solid culture medium having a pH of 5.0-5.75 to redifferentiate a plantlet; and (e) acclimatizing the redifferentiated plantlet in a soil where bed soil and sand are mixed in a ratio of 1:1, wherein the culture medium of the step(a) is an MS culture medium where 2,4-D or NAA is further added as the plant growth controlling material. Further, the MS culture medium comprises 10-30 mg/l of sucrose as carbon source.
Abstract translation: 提供了一种大规模生产Oplopanax elatus Nakai小植物的方法，有效地从Oplopanax elatus Nakai组织的胚胎发生愈伤组织中大量繁殖Oplopanax elatus Nakai，从而保存濒危的Oplopanax elatus Nakai。 一种大规模生产Oplopanax elatus Nakai小植物的方法包括以下步骤：（a）在MS培养基中移除Oplopanax elatus Nakai的组织段，其中加入包含TDZ的植物生长控制材料以诱导胚胎发生愈伤组织 ; （b）在液体培养基或固体培养基中培养诱导的胚胎发生愈伤组织以诱导体细胞胚; （c）在液体培养基或固体培养基中培养诱导的体细胞胚以繁殖它们; （d）在液体培养基或pH5.0-5.75的固体培养基中培养繁殖的体细胞胚以再分化植物; 和（e）将所述再分化的小植物驯化在比例为1:1的床土壤和沙子的土壤中，其中步骤（a）的培养基是MS培养基，其中2,4-D或NAA为 进一步添加作为植物生长控制材料。 此外，MS培养基包含10-30mg / l蔗糖作为碳源。
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公开(公告)号：KR1020070105812A
公开(公告)日：2007-10-31
申请号：KR1020060067626
申请日：2006-07-19
Applicant: 강원대학교산학협력단
IPC: A61K36/185 , A23L1/29
Abstract: An extract of Hibiscus cannabinus L.(Malvaceae) and a novel kaempferol compound isolated therefrom are provided to inhibit growth of microorganisms, oxidation and aging in the skin without side effects, so that they are useful in medicines, cosmetics and functional food for improving skin beauty. An extract of Hibiscus cannabinus L. having improved antimicrobial and antioxidizing effects is prepared by extracting Hibiscus cannabinus L. with a solvent selected from water, aliphatic alcohol or alcohol solution, hexane, chloroform and ethylacetate. A novel kaempferol compound represented by the formula(1) is isolated from the extract of Hibiscus cannabinus L. by extracting Hibiscus cannabinus L. with methanol, extracting the crude methanol extract with water, aliphatic alcohol, alcohol solution, hexane, chloroform or ethylacetate to obtain a water layer and an organic layer, and subjecting the water layer and organic layer to silica chromatography, gel filtration chromatography and HPLC(high performance liquid chromatography), sequentially.
Abstract translation: 提供木槿大麻提取物和从其中分离出的新型山奈酚化合物，以抑制微生物的生长，皮肤的氧化和老化，而不会有副作用，因此它们可用于改善皮肤的药物，化妆品和功能性食品 美丽。 通过用选自水，脂族醇或醇溶液，己烷，氯仿和乙酸乙酯的溶剂萃取木槿大麻制备具有改善的抗微生物和抗氧化作用的木槿提取物。 用甲醇提取木槿大麻，从木槿提取物中分离出由式（1）表示的新型山奈酚化合物，用水，脂肪醇，醇溶液，己烷，氯仿或乙酸乙酯提取粗甲醇提取物， 得到水层和有机层，依次将水层和有机层进行硅胶色谱，凝胶过滤色谱和HPLC（高效液相色谱）。
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Patent Agency Ranking
公开(公告)号：KR1020070105811A
公开(公告)日：2007-10-31
申请号：KR1020060067602
申请日：2006-07-19
Applicant: 강원대학교산학협력단
Abstract: Healthy tea with kenaf leaves and a preparation method thereof are provided to facilitate the elution of active ingredients in extraction in hot water by treating the leaves with heat and far infrared ray in a fixed condition, thereby substituting the same for green tea. Healthy tea with kenaf leaves is prepared by the steps of: heating kenaf leaves at 60~100°C for 10~45min; and applying far infrared ray to the leaves at 1.6kw for 10~45min while keeping the temperature at 60~100°C. In the preparation process, large quantities of minerals and antioxidant ingredients are extracted from the kenaf leaves. The extract is especially rich in kaempferols as a polyphenol compound. In addition, the content of physiologically active ingredients in the kenaf leaves is higher than that in green tea leaves.
Abstract translation: 提供具有洋麻叶的健康茶及其制备方法，以便通过用固定状态的热和远红外线处理叶子以促进在热水中萃取中的活性成分的洗脱，从而将其替换为绿茶。 通过以下步骤制备具有红麻叶的健康茶：在60〜100℃下加热洋麻叶10〜45分钟; 并在1.6kw的条件下将远红外线应用10〜45min，同时保持温度在60〜100℃。 在制备过程中，从洋麻叶中提取大量的矿物质和抗氧化成分。 提取物特别富含作为多酚化合物的山奈酚。 此外，洋麻叶中生理活性成分的含量高于绿茶叶。
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公开(公告)号：KR101445276B1
公开(公告)日：2014-09-26
申请号：KR1020130029800
申请日：2013-03-20
Applicant: 강원대학교산학협력단
CPC classification number: A23L17/60 , A23D9/00 , A23L33/105 , A61K8/922
Abstract: The present invention relates to a method for producing laver and a use of the same, wherein Pinus Koraiensis oil (phytoncide) produced from a Pinus Koraiensis byproduct is mixed with Canola oil, olive oil, sesame oil, cone oil, bean oil, grape seed oil, sunflower oil, perilla seed oil, avocado oil, almond oil, garlic oil, safflower seed oil, pumpkin seed oil, linseed oil, castor oil, rape seed oil, brown rice oil, coconut oil, peanut oil, cotton seed oil, and cocktail oil in order to be coated on the laver. In particular, the method of the present invention comprises a step of producing mixed oil by mixing edible oil with the Pinus Koraiensis oil (phytoncide) extracted from a pine cone or a pine leaf using water or an organic solvent. The laver coated with the Pinus Koraiensis oil has excellent anti-microorganism activities, and the oil of the laver has an extended acidification period. Accordingly, the shelf life is extended, and the laver of the present invention can be widely used as health food which adds value to a company.
Abstract translation: 本发明涉及一种生产紫菜的方法及其用途，其中由科罗拉松子副产品生产的松属油（phyorcide）与卡诺拉油，橄榄油，芝麻油，锥形油，豆油，葡萄籽混合 油，向日葵油，紫苏籽油，鳄梨油，杏仁油，大蒜油，红花籽油，南瓜籽油，亚麻籽油，蓖麻油，油菜籽油，糙米油，椰子油，花生油，棉籽油， 和鸡尾酒油，以便涂在紫菜上。 特别地，本发明的方法包括通过使用水或有机溶剂将食用油与从松果或松树叶提取的松属油（phytoncide）混合来生产混合油的步骤。 含有松油松油的紫菜具有优异的抗微生物活性，紫菜油具有延长的酸化期。 因此，保质期延长，本发明的紫菜可广泛用作为公司增值的保健食品。
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公开(公告)号：KR101300509B1
公开(公告)日：2013-08-26
申请号：KR1020110090866
申请日：2011-09-07
Applicant: 강원대학교산학협력단
Abstract: 본 발명은 억새 (Miscanthus sinensis) 유래의 COMT (caffeic acid O-methyltransferase) 유전자를 포함하는 재조합 벡터를 식물세포에 형질전환시켜 COMT 유전자의 발현을 조절하는 단계를 포함하는 식물의 페놀계 화합물 함량을 조절하는 방법, 억새 유래의 COMT 유전자를 포함하는 재조합 벡터로 식물세포를 형질전환하는 단계; 및 상기 형질전환된 식물세포로부터 식물을 재분화하는 단계를 포함하는 식물의 페놀계 화합물의 함량이 조절된 식물체의 제조 방법, 상기 방법에 의해 제조된 페놀계 화합물의 함량이 야생형에 비해 조절된 형질전환 식물체 및 이의 종자 및 억새 유래의 COMT 유전자를 포함하는, 식물체의 페놀계 화합물 함량 조절용 조성물을 제공한다.
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19.发明授权비타민나무 잎으로부터 분리한 이소람네틴-3-글루코시드-7-람노시드를 유효성분으로 함유하는 항산화용 조성물 有权包含从海马RHAMNOIDES LEAVES分离的异山梨醇-3-GLUCOSIDE-7- RHAMNOSIDE的抗氧化剂组合物
公开(公告)号：KR101121590B1
公开(公告)日：2012-03-06
申请号：KR1020110073986
申请日：2011-07-26
Applicant: 삼성생약 주식회사 농업회사법인 , 강원대학교산학협력단
IPC: A61K31/351 , A61K31/352 , A61K31/7048 , A61P35/00
CPC classification number: A61K31/7048 , A61K31/352
Abstract: PURPOSE: An anti-oxidative composition containing isorhamnetin-3-glucoside-7-rhamnoside isolated from Hippophae rhamnoides L. is provided to ensure antioxidative, antibacterial, and anti-cancer effect. CONSTITUTION: A cosmetic composition for preventing or treating aging contains irorhamnetin-3-glucoside-7-rhamnoside(IGR) compound as an active ingredient. The compound of chemical formula 1 is isolated from Hippophae rhamnoides L. leaves. A health food composition for antioxiaiton contains the compound of chemical formula 1 as an active ingredient. A pharmaceutical composition for antibacterial and anticancer contains the compound of chemical formula 1 as an active ingredient.
Abstract translation: 目的：提供从沙棘沙门氏菌分离得到的含有异鼠李素-3-葡萄糖苷-7-鼠李糖苷的抗氧化组合物，以确保抗氧化，抗菌和抗癌作用。 构成：用于预防或治疗老化的化妆品组合物含有作为活性成分的伊仑汉甜素-3-葡萄糖苷-7-鼠李糖苷（IGR）化合物。 化学式1的化合物从沙棘沙门氏菌分离。 抗氧化剂的保健食品组合物含有化学式1的化合物作为活性成分。 用于抗菌和抗癌的药物组合物含有化学式1的化合物作为活性成分。
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20.发明公开스티그마스트―４―엔―３，６―디온을 유효성분으로 함유하는 신경계 질환 예방 및 치료용 조성물 有权用于预防和治疗含有STIGMAST-4-EN-3,6-DIONE作为有效成分的神经系统疾病的组合物
公开(公告)号：KR1020110116558A
公开(公告)日：2011-10-26
申请号：KR1020100036040
申请日：2010-04-19
Applicant: 강원대학교산학협력단
CPC classification number: A61K31/575 , A23L29/035 , A23V2200/322 , A61K36/22 , Y10S514/903
Abstract: 본 발명은 하기 [화학식 1]로 기재되는 화합물을 유효성분으로 함유하는 신경계 질환의 예방 및 치료용 조성물에 관한 것으로, 구체적으로 옻에 함유된 알러지 유발성분을 저감화시킨 발효된 옻나무 수피(
Rhus
verniciflua Barks)를 유기용매로 추출 및 분획하여 분획물을 얻고, 상기 획득한 분획물에서 분리한 화합물인 스티그마스트-4-엔-3,6-디온(stigmast-4-en-3,6-dione)을 포함하는 신경계 질환의 예방 및 치료용 조성물에 관한 것이다. 본 발명의 조성물은 신경 세포의 보호 활성을 나타내므로, 신경계 질환의 예방 또는 치료용 조성물로 유용하게 사용될 수 있다:
[화학식 1]
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