Abstract:
건강기능성 식품소재로 활용되는 안토시아닌 성분은 각종 과일, 열매, 채소를 비롯해 검정콩 껍질부분에 많이 함유하고 있다. 따라서 기존 유기용매를 이용한 안토시아닌 추출방법은 안전성, 경제적, 시간적 제한을 받고 있다. 따라서 보조용매(물/에탄올)를 이용한 이산화탄소 초임계 추출방법은 상기의 문제점을 해결할 수 있는 실용적인 방법으로서, 이러한 안토시아닌 추출방법은 본 발명의 소재로 활용된 검정콩뿐만 아니라 안토시아닌을 함유하는 과일, 채소, 곡류 등에 활용 가능한 방법으로 그 실용성이 매우 크다. 현재까지 이산화탄소 초임계 추출법은 비극성물질인 지방, 지방산, 왁스, 글리세롤, 토코페롤, 인지질 및 각종 향기성분 등을 추출/정제하는 용도로 이용되고 있다. 따라서, 본 발명은 극성물질인 안토시아닌 성분을 추출하기 위해 보조용매인 물/에탄올을 초임계 공정에 도입한 기술 및 반응조에서 추출효율을 높이기 위해 교반창치의 도입부분이 기존 유기용매 추출법에 비하여 추출효율을 향상 시켰다.
Abstract:
PURPOSE: A manufacturing method of a bean composition containing conjugated linoleic acid is provided to have the bean composition containing conjugated linoleic acid by solid fermenting a wet grinding material of bean after inoculating a mold or a mushroom mycelium. CONSTITUTION: A wet grinding material of bean which has 20-80 weight% of moisture content is manufactured by performing a water-logging and grinding the bean. A wet grinding material of bean is boiled to annihilate various germs. A boiled wet grinding material of bean is cooled and inoculated with a microorganism. A inoculated grinding material of bean is fermented at 10-50 °C for 7-30 days. The microorganism is made by cultivating a spawn after inoculating in a liquid culture medium in which rice and bean are mixed. The microorganism is Eumycetes or the mushroom mycelium. The wet grinding material of bean and microorganism is mixed as a ratio of (80-100) : (1-20) parts by weight. A linoleic acid or the cooking oil is additionally added in the grinding material of bean. The grinding material of bean is wet grinding material of bean which has 20-80 weight% of moisture content or a liquid grinding material in which the bean and moisture have a ratio of (6 - 20) parts by weight. [Reference numerals] (AA) Domestic bean raw material (variety: Deapung, Deauwl); (BB) Soaking in water and grinding; (CC) Manufacturing soybean milk; (DD) Cultivating on mold or mushroom mycelium; (EE,FF) Inoculation; (GG) Cultivating lactobacillus; (HH) Solid fermentation; (II) Lactobacillus fermentation; (JJ) Bean processed product with CLS; (KK) Bean yogurt with CLS
Abstract:
PURPOSE: A method for extracting anthocyanin is provided to control temperature and pressure conditions to maintain anthocyanin functionality and to be used for various industrial fields as food, pharmaceutical, and cosmetic materials. CONSTITUTION: A supercritical extraction method for extracting anthocyanin ingredients from black soybean powder comprises a step of extracting while stirring carbon dioxide of a supercritical state using water and alcohol under an acidic condition. The supercritical state is a temperature of 30-60 deg. C and a pressure of 80-300 bar. An apparatus for extracting anthocyanin comprises a cooler(120), a high pressure liquid pump(130), a reactor(180), a primary separation tank(200), and a secondary separation tank(230). The cooler changes a phase into a liquid phase by cooling carbon dioxide from CO_2 container(100). The high pressure liquid pump receives and pressurizes carbon dioxide. The reactor heats and stirs a reactant of the black soybean powder, water, ethanol, and organic acid in an inner receiving unit by mounting a magnetic driving device(170) for stirring and a heating device. The primary and secondary separation tanks separate carbon dioxide and the reactant by spraying the reactant to a low pressure container and has a filter(210).
Abstract:
PURPOSE: An isoflavone content increasing method inside a bean seed is provided to use a lighting process to beans during the vegetative period of the beans. CONSTITUTION: An isoflavone content increasing method inside a bean seed includes a step of light-processing beans during the vegetative period of the beans. The intensity of the lighting process is 20~50lux. The lighting process is performed for 10~30days, 1~4hours after the sunrise and 1~3hours after the sunset. The beans are glycine max.