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公开(公告)号：KR1020060132155A
公开(公告)日：2006-12-21
申请号：KR1020050052325
申请日：2005-06-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/145 , A61K39/12
CPC classification number: A61K39/145 , A61K38/02 , Y10S514/888
Abstract: A low pathogenic avian influenza viral vaccine for preventing H5 subtype avian influenza viral infection is provided to enhance safety and medicinal efficacy of use, and prevent the infection of H5N1 type high pathogenic avian influenza virus safely. The method for preparing the low pathogenic avian influenza viral vaccine for preventing H5 subtype avian influenza viral infection comprises the steps of: (1) culturing a A/wild bird feces/Korea/CSM-2/02(H5N3) strain in a specific pathogen free egg(SPF egg) for 72 hours and collecting allantoic fluid; (2) measuring the titrate of the virus collected in step(1) through hemagglutination inhibition test; (3) concentrating the virus 10 times by centrifuging the virus at 18,000 rpm for 3 hours; (4) attenuating the virus by treating the concentrated virus with 0.1% formalin at 20 deg.C for 12 hours; and (5) mixing the attenuated virus solution with oil adjuvant ISA70 in a weight ratio of 3:7, wherein the low pathogenic avian influenza viral vaccine contains at least one protein encoded by the gene of SEQ ID NO:2 to SEQ ID NO:9.
Abstract translation: 提供用于预防H5亚型禽流感病毒感染的低致病性禽流感病毒疫苗，以提高使用的安全性和药用效力，并安全防止H5N1型高致病性禽流感病毒感染。 制备用于预防H5亚型禽流感病毒感染的低致病性禽流感病毒疫苗的方法包括以下步骤：（1）在特定病原体中培养A /野鸟粪/韩国/ CSM-2/02（H5N3）菌株 免费鸡蛋（SPF蛋）72小时并收集尿囊液; （2）通过血细胞凝集抑制试验测定步骤（1）中收集的病毒的滴度; （3）通过以18,000rpm将病毒离心3小时来浓缩病毒10次; （4）通过用0.1％福尔马林在20℃处理浓缩的病毒12小时来减毒病毒; 和（5）将减毒病毒溶液与油佐剂ISA70以3:7的重量比混合，其中低致病性禽流感病毒疫苗含有由SEQ ID NO：2至SEQ ID NO：2的基因编码的至少一种蛋白质。 9。
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公开(公告)号：KR101617142B1
公开(公告)日：2016-05-02
申请号：KR1020140060752
申请日：2014-05-21
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 인트론바이오테크놀로지
Abstract: 본발명은개선된검출정확성을제공해줄 수있는핵산검사기반의조류인플루엔자바이러스검출및 H5형, H7형, 및 H9형동시감별검출키트에관한것이다. 본발명에따른조류인플루엔자바이러스검출및 H5형, H7형, 및 H9형동시감별검출키트는조류인플루엔자바이러스의목적유전자에서의짧은보존서열로인한문제점을극복할수 있는서열번호 1 내지서열번호 16으로특징지어지는특이프라이머들이적용되고, 또한기존조류인플루엔자바이러스검출용다중유전자증폭에서의민감도저하및 위음성발생문제를회피하기위하여 8개의튜브로구성된다중튜브방식이적용된것을특징으로한다. 본발명의조류인플루엔자바이러스검출및 H5형, H7형및 H9형을동시감별검출키트는조류인플루엔자바이러스검출과임상적으로주요한조류인플루엔자바이러스 H5형, 조류인플루엔자 H7형및 조류인플루엔자 H9형의동시감별검출에있어유용하게활용될수 있다.
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公开(公告)号：KR101423695B1
公开(公告)日：2014-08-04
申请号：KR1020120076916
申请日：2012-07-13
Applicant: 서울대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 저병원성 조류 인플루엔자 바이러스의 PB2 단백질 코딩 폴리뉴클레오타이드를 유효성분으로 포함하는 H1N1 계통 조류 인플루엔자 바이러스 발육계란 고증식성, 마우스 무병원성 조성물, 상기 폴리뉴클레오타이드를 포함하는 재조합 발현 벡터, 상기 발현 벡터로 형질전환 된 약독화 재조합 바이러스, 및 상기 재조합 바이러스를 포함하는 백신 조성물에 관한 것이다. 본 발명의 재조합 발현 벡터는 인플루엔자 예방을 위한 백신주의 계태아 생산성을 높여주어 사독백신 효능 및 생산성 향상에 유용하게 이용될 수 있고, 마우스에 대한 병원성이 없고, 면역원성이 있어 생독백신에 활용될 수 있다는 장점을 갖는다.
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公开(公告)号：KR1020140078523A
公开(公告)日：2014-06-25
申请号：KR1020130075529
申请日：2013-06-28
Applicant: 조지아 스테이트 유니버시티 리서치 파운데이션, 인코포레이티드 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C07K14/11 , C12N7/01 , A61K39/145 , A61P31/16
Abstract: The present invention relates to an influenza monovalent vaccine having a broad defending activity, or an influenza supplementary vaccine using the same. The influenza monovalent vaccine or influenza supplementary vaccine of the present invention increases the immunity against an M2e protein for influenza virus infection of various serotypes, thereby having a broad defense ability effectively responding to the unforeseen spread of virus like national epidemic.
Abstract translation: 本发明涉及具有广泛防御活性的流感单价疫苗，或使用其的流感病毒补充疫苗。 本发明的流感性单价疫苗或流感病毒补充疫苗提高了针对各种血清型流感病毒感染的M2e蛋白的免疫力，从而具有有效应对国家流行病毒等不可预见的病毒传播的广泛防御能力。
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公开(公告)号：KR101911017B1
公开(公告)日：2018-10-25
申请号：KR1020170098623
申请日：2017-08-03
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 메디안디노스틱
IPC: C12Q1/68
CPC classification number: C12Q1/689 , C12Q1/6883 , C12Q2531/113
Abstract: 본발명은타일로렐라에퀴제니탈리스의검출용조성물, 타일로렐라에퀴제니탈리스감염에의한말전염성자궁염의진단용조성물, 타일로렐라에퀴제니탈리스의검출방법, 및타일로렐라에퀴제니탈리스감염에의한말전염성자궁염의진단방법에관한것으로, 보다구체적으로는말전염성자궁염(Contagious Equine Metritis, CEM)의원인균인타일로렐라에퀴제니탈리스()의 16S rRNA 유전자염기서열에상보적인염기서열을포함하는프라이머쌍을유효성분으로포함하는조성물, 상기조성물을포함하는키트, 및상기프라이머쌍을이용하는타일로렐라에퀴제니탈리스의검출방법과타일로렐라에퀴제니탈리스감염에의한말전염성자궁염의진단방법에관한것이다.
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Patent Agency Ranking16.发明授权조류인플루엔자 바이러스 검사용 프로브 조성물, 유전자 칩 그리고 이를 이용한 조류인플루엔자 바이러스 검사방법 有权用于亚型禽流感病毒的探针和DNA芯片以及使用该禽流感病毒检测禽流感病毒的方法
公开(公告)号：KR101592247B1
公开(公告)日：2016-02-05
申请号：KR1020120039157
申请日：2012-04-16
Applicant: 바이오코아 주식회사 , 건국대학교 산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본발명은조류인플루엔자바이러스의혈청형을결정하는 HA 유전자의아형 (H5형, H7형, H9형) 및 NA 유전자의아형(N1형, N2형, N3형) 판별과병원성의검사가동시에가능하면서도조류인플루엔자바이러스를조기에신속하고정확하게검사할수 있는조류인플루엔자바이러스검사용프로브조성물, 유전자칩 그리고이를이용한조류인플루엔자바이러스검사방법을제공한다. 본발명에따르면, 저가의비용으로 5시간이내에고병원성조류인플루엔자바이러스의혈청형및 병원성감별이가능하고저병원성 H9형바이러스의감별과더불어경제적이며빠른진단으로고병원성조류인플루엔자발생시조기진단에따른초동대처로질병발생피해를최소화하는데기여할수 있다.
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公开(公告)号：KR1020140010583A
公开(公告)日：2014-01-27
申请号：KR1020120076917
申请日：2012-07-13
Applicant: 서울대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/01 , C12N15/63 , C12N15/33 , A61K39/145
Abstract: Provided are: a method for improving immunogenicity of a HA2 common epitope of influenza virus by replacing asparagine, which is the 154th amino acid, and serine (S) or threonine (T), which is the 156the amino acid, with other amino acid such that N-glycan generation does not occur at asparagine (N), which is the 154th amino acid, of a HA2 fragment derived from a HA amino acid of avian influenza virus; and a recombinant virus with improved immunogenicity through the method. The technic for improving immunogenicity of a HA2 common epitope and the HA2 common epitope high immunogenicity virus of the present invention can be effectively used in developing a vaccine strain for preventing influenza and effectively used as a vaccine strain.
Abstract translation: 提供：通过将作为第154位氨基酸的天冬酰胺和作为氨基酸的156位的丝氨酸（S）或苏氨酸（T）与其它氨基酸这样的氨基酸取代来改善流感病毒的HA2常见表位的免疫原性的方法 来自禽流感病毒的HA氨基酸的HA2片段的第155位氨基酸的天冬酰胺（N）不发生N-聚糖生成; 和通过该方法具有改善的免疫原性的重组病毒。 用于提高本发明的HA2常见表位和HA2常见表位高免疫原性病毒的免疫原性的技术可以有效地用于开发用于预防流行性感冒并有效用作疫苗株的疫苗株。
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公开(公告)号：KR1020140010582A
公开(公告)日：2014-01-27
申请号：KR1020120076916
申请日：2012-07-13
Applicant: 서울대학교산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: The present invention relates to: a composition for high productiveness of an embryonated egg of H1N1 family avian influenza and non-pathogenicity of a mouse including, as a active ingredient, a polynucleotide encoding a PB2 protein of a low pathogenic avian influenza virus; a recombinant expression vector including the polynucleotide; an attenuated recombinant virus transformed into the expression vector; and a vaccine composition including the recombinant expression vector. The recombinant expression vector of the present invention enhances chicken embryo productivity of a vaccine strain for preventing influenza, and thereby, having advantages of being capable of being effectively used in enhancing efficacy and productivity of killed vaccines, and additionally, has no pathogenicity for mice and has immunogenicity, and thereby having advantages of being capable of being effectively used as live vaccines. [Reference numerals] (AA) Inserting into the inside of pHW2000-BsmBI
Abstract translation: 本发明涉及一种用于H1N1家禽禽流感的胚胎卵和非致病性的高生产力的组合物，其包含作为活性成分的编码低致病性禽流感病毒的PB2蛋白的多核苷酸; 包含该多核苷酸的重组表达载体; 减毒的重组病毒转化成表达载体; 以及包含重组表达载体的疫苗组合物。 本发明的重组表达载体增强用于预防流感的疫苗株的鸡胚生产力，从而具有能够有效地用于提高杀死疫苗的功效和生产力的优点，此外，对小鼠没有致病性， 具有免疫原性，因此具有能够有效地用作活疫苗的优点。 （标号）（AA）插入pHW2000-BsmBI的内部
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公开(公告)号：KR101144993B1
公开(公告)日：2012-06-27
申请号：KR1020090054422
申请日：2009-06-18
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: An antigen for hemoglutination for swine Japanese encephalitis virus is provided to reduce pollution caused by acetone extraction. CONSTITUTION: A method for preparing antigen for hemoglutination of high concentration for swine Japanese encephalitis virus comprises: a step of inoculating swine Japanese encephalitis virus to a tissue culture cells and planting; a step of freezing culture liquid; a step of thawing the culture liquid and repeating freezing and thawing once; a step of collecting cultured swine Japanese encephalitis virus; a step of inactivating swine Japanese encephalitis virus; a step of concentrating swine Japanese encephalitis virus; and a step of precipitating swine Japanese encephalitis virus by centrifugation.
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20.发明公开신속 면역크로마토그라피법에 의한 Ｈ9형 조류인플루엔자 바이러스 진단 키트 및 이를 이용하여 Ｈ9형 조류인플루엔자를 진단하는 방법 有权使用快速免疫印迹的H9型AVIAN流感病毒的诊断试剂盒和使用该方法诊断H9型AVIAN流感的方法
公开(公告)号：KR1020120024313A
公开(公告)日：2012-03-14
申请号：KR1020100087163
申请日：2010-09-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 바이오노트
IPC: G01N33/569 , G01N33/538 , G01N33/553 , C07K16/08
Abstract: PURPOSE: An H9 type avian influenza virus diagnosis kit by quick immunochromatography is provided to ensure quick and simple diagnosis without special equipment. CONSTITUTION: A kit for diagnosing H9 type avian influenza virus contains monoclonal antibodies. The monoclonal antibody is prepared from hybridoma cells of deposit number 11753BP, KCTC 11754BP, and KCTC 11755BP. A H9 type avian influenza diagnosis strip contains the monoclonal antibody. The diagnosis strip comprises a sample pad(1), a gold pad(2), nitrocellulose membrane(3), and moisture absorption pad(4). The gold pad contains a gold conjugate in which gold particles are conjugated with a monoclonal antibody produced from hybridoma cells of deposit number KCTC 11755BP.
Abstract translation: 目的：提供快速免疫色谱法的H9型禽流感病毒诊断试剂盒，无需特殊设备即可快速，简便地进行诊断。 构成：用于诊断H9型禽流感病毒的工具包含单克隆抗体。 单克隆抗体由编号11753BP，KCTC 11754BP和KCTC 11755BP的杂交瘤细胞制备。 H9型禽流感诊断条含有单克隆抗体。 诊断条包括样品垫（1），金垫（2），硝酸纤维素膜（3）和吸湿垫（4）。 金垫包含金结合物，其中金颗粒与由保藏号为KCTC 11755BP的杂交瘤细胞产生的单克隆抗体缀合。
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