公开(公告)号：KR1020110136441A

公开(公告)日：2011-12-21

申请号：KR1020100056459

申请日：2010-06-15

Applicant: 삼성전자주식회사 ,  고려대학교 산학협력단

Inventor： 구현민 ,  박성민 ,  박재찬 ,  김경헌 ,  최인걸

IPC: C12Q1/26 ,  G01N33/52 ,  G01N30/96

CPC classification number: C12Q1/48 ,  C12N9/1051 ,  G01N2333/91102

Abstract: PURPOSE: A composition for measuring 3,6-anhydro-L-galactose transferase activity is provided to be used in an industry. CONSTITUTION: A composition for measuring 3,6-L-AHG transferase contains NADP+ as a coenzyme, 3,6-anhydro-L-galactose(3,6-L-AHG), and buffer solution. A method for measuring the activity of 3,6-L-AHG transferase comprises: a step of reacting random enzyme extract liquid and a composition for measuring the activity of 3,6-L-AHG tranferase; a step of measuring reduction into NADPH by the reaction. A composition for quantitation of 3,6-L-AHG contains an active fraction containing 3,6-L-AHG tranferase, NADP+ as a coenzyme, and buffer solution.

Abstract translation: 目的：用于测量3,6-脱水-L-半乳糖转移酶活性的组合物用于工业中。 构成：用于测量3,6-L-AHG转移酶的组合物含有作为辅酶的NADP +，3,6-脱水-L-半乳糖（3,6-L-AHG）和缓冲溶液。 测定3,6-L-AHG转移酶的活性的方法包括：使随机酶提取液与用于测定3,6-L-AHG转移酶活性的组合物反应的步骤; 通过反应测量还原成NADPH的步骤。 用于定量3,6-L-AHG的组合物含有含有3,6-L-AHG转移酶，NADP +作为辅酶的活性级分和缓冲溶液。

公开(公告)号：KR1020130094115A

公开(公告)日：2013-08-23

申请号：KR1020120015526

申请日：2012-02-15

Applicant: 삼성전자주식회사

Inventor： 이영민 ,  구현민 ,  김재영 ,  김진우 ,  박재찬 ,  박준성 ,  조화영 ,  이평천

IPC: C12N1/21 ,  C12P7/16 ,  C12N15/52 ,  C12N15/63

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/13 ,  C12N9/88 ,  C12Y101/01001 ,  C12Y101/01016 ,  C12Y102/01016 ,  C12Y102/04002 ,  C12Y203/01019 ,  C12Y207/02007 ,  C12Y208/03005 ,  C12Y401/01071

Abstract: PURPOSE: A deformed microorganism for production of 1,4-butanediol is provided to produce 1,4-BDO by a biological producing process. CONSTITUTION: A deformed microorganism for production of 1.4-butanediol comprises: an activation converting alpha-ketoglutarate or succinate into 4-hydroxybutyril-CoA ("4-HB-CoA"); and an activation converting the 4-HB-CoA into 1,4-butanediols ("1,4-BDO"). The activation converting the 4-HB-CoA into the 1,4-BDO is coded by more than one kind of genes selected from a group comprising adh1, yiaY, adh4, adhB, mdh, eutG, fucO, dhaT, aldA, eutE, adhE1, adhE2 and adh2.

Abstract translation: 目的：提供用于生产1,4-丁二醇的变形微生物，以通过生物制备方法生产1,4-BDO。 构成：用于生产1,4-丁二醇的变形的微生物包括：将α-酮戊二酸或琥珀酸转化成4-羟基丁酰辅酶A（“4-HB-CoA”）的活化; 和将4-HB-CoA转化为1,4-丁二醇（“1,4-BDO”）的活化。 将4-HB-CoA转化为1,4-BDO的激活由选自包含adh1，yyY，adh4，adhB，mdh，eutG，fucO，dhaT，aldA，eutE， adhE1，adhE2和adh2。

公开(公告)号：KR1020130000688A

公开(公告)日：2013-01-03

申请号：KR1020110061366

申请日：2011-06-23

Applicant: 삼성전자주식회사

Inventor： 조화영 ,  유병조 ,  박재찬 ,  박성민 ,  구현민 ,  이주영

IPC: C12Q1/04 ,  C12N1/20 ,  C12N1/14 ,  C12P7/56

CPC classification number: G01N21/80 ,  C12Q1/025

Abstract: PURPOSE: A high throughput screening system and a method of lactic acid-producing strains using two or more pH indicators are provided to quickly and accurately screen strains. CONSTITUTION: A high throughput screening system of lactic acid-producing strains contains the strain, a medium, and two or more pH indicators. The strains are a wild type strain, a mutant strain, and a recombinant strain. The pH indicators contain bromocresol green and methyl red in a ratio of 2:1-10:1. A method for screening the lactic acid-producing strains comprises: a step of adding the pH indicators to a medium; a step of culturing the strains; and a step of observing color change of the pH indicators, and measuring lactic acid production quantity.

Abstract translation: 目的：提供高通量筛选系统和使用两种或多种pH指示剂的乳酸生产菌株的方法，以快速准确筛选菌株。 构成：乳酸生产菌株的高通量筛选系统含有菌株，培养基和两种或多种pH指示剂。 菌株是野生型菌株，突变菌株和重组菌株。 pH指标含有溴甲酚绿和甲基红，比例为2：1-10：1。 筛选乳酸生产菌株的方法包括：将pH指示剂加入到培养基中的步骤; 培养菌株的一个步骤; 以及观察pH指示剂的变色，测定乳酸生成量的工序。

公开(公告)号：KR101860442B1

公开(公告)日：2018-05-24

申请号：KR1020110062312

申请日：2011-06-27

Applicant: 삼성전자주식회사

Inventor： 김재영 ,  박성민 ,  박영경 ,  유병조 ,  박재찬 ,  조화영 ,  구현민

IPC: C12N15/53 ,  C12N15/63 ,  C12P7/52

CPC classification number: C12P7/42 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y203/01008

Abstract: Malonic Semialdehyde 환원경로를이용하여 3-하이드록시프로피온산(3-HP)을생산하는데 있어서, 락테이트탈수소화효소(lactate dehydrogenase), 포스포트란스아세틸라아제(phosphotransacetylase) 및알코올탈수소화효소(alcohol dehydrogenase)의발현을저해함으로써중간대사산물의유출(metabolite leak)을막아 Malonyl-CoA 풀(pool)을증가시켜 3-HP 생성효율을현저히높일수 있는방법에관한것이다.

公开(公告)号：KR101744341B1

公开(公告)日：2017-06-09

申请号：KR1020100056459

申请日：2010-06-15

Applicant: 삼성전자주식회사 ,  고려대학교 산학협력단

Inventor： 구현민 ,  박성민 ,  박재찬 ,  김경헌 ,  최인걸

IPC: C12Q1/26 ,  G01N33/52 ,  G01N30/96

CPC classification number: C12Q1/48 ,  C12N9/1051 ,  G01N2333/91102

Abstract: NADP에서 NADPH로의환원을통해 L-AHG 전환효소의활성을측정하는, 3,6-안하이드로-L-갈락토오스전환효소의활성측정용조성물및 이를이용한 3,6-안하이드로-L-갈락토오스전환효소의활성측정방법을제공한다. 해조류바이오매스등 3,6-안하이드로-L-갈락토오스를포함하는원료의이용에유용하게사용될수 있는바 산업적으로유용하다.

公开(公告)号：KR1020130124065A

公开(公告)日：2013-11-13

申请号：KR1020120047683

申请日：2012-05-04

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  구현민 ,  박성민 ,  권대혁 ,  김재영 ,  박재찬

IPC: C12N1/19 ,  C12N15/55 ,  C12P7/06 ,  C12N15/81

CPC classification number: Y02E50/17 ,  C12N15/815 ,  C12N9/2402 ,  C12P7/10 ,  C12P7/40 ,  C12P13/04 ,  C12P19/02 ,  C12P19/04

Abstract: Disclosed is modified microorganisms for simultaneous saccharification and fermentation, an expression vector for preparing the modified microorganisms, and a method for producing chemical materials using the modified microorganisms. According to one side, disclosed is modified Kluyveromyces marxianus which produces chemical materials by simultaneous saccharification and fermentation and contains a replication origin; a promoter; a gene encodes one or more cellulose decomposing enzymes selected among beta-glucosidase, endoglucanase, exoglucanase, and cellobiohydrolase; and a terminator.

Abstract translation: 公开了用于同时糖化和发酵的改性微生物，用于制备改性微生物的表达载体，以及使用改性微生物生产化学物质的方法。 一方面，公开了通过同时进行糖化和发酵生产化学物质的马克斯克鲁维酵母（Kluyveromyces marxianus），并含有复制起点; 启动子 一种基因编码一种或多种选自β-葡糖苷酶，内切葡聚糖酶，外切葡聚糖酶和纤维二糖水解酶的纤维素分解酶; 和终结者。

公开(公告)号：KR1020100047407A

公开(公告)日：2010-05-10

申请号：KR1020080106278

申请日：2008-10-29

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박재찬 ,  구현민 ,  진용수 ,  이기성

IPC: C12N15/52 ,  C12N15/56 ,  C12N15/09

CPC classification number: C12N15/1034 ,  C07K14/395 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/28 ,  Y02E50/10 ,  Y02E50/17 ,  C12N15/52 ,  C12N9/24 ,  C12N15/09

Abstract: PURPOSE: An SNR84 gene enhancing usability of galactose metabolism is provided to enhance metabolic rate of galactose and enhance productivity of bioalcohol from carbon source. CONSTITUTION: An SNR84 gene enhances galactose metabolism rate. The gene is denoted by sequence number 1. A pRS424 recombinant vector contains the SNR84 gene of sequence number 1. A transformed recombinant microorganism is obtained using the recombinant vector. The recombinant microorganism overexpresses the SNR84 gene. The microorganism is yeast. The yeast is Saccharomyces sp., Pachysolen sp., Clavispora sp., Kluyveromyces sp., Debaryomyces sp., Schwanniomyces sp., Candida sp., Pichia sp., or Dekkera sp. The deposit number KCTC 11388 BP of the recombinant microorganism is CEN.PK2-1D/pRS424-SNR84.

Abstract translation: 目的：提供增强半乳糖代谢可用性的SNR84基因，以提高半乳糖的代谢率，并提高生物醇从碳源的生产力。 构成：SNR84基因增强半乳糖代谢率。 该基因由序列号1表示.PRS424重组载体含有序列号1的SNR84基因。使用重组载体获得转化的重组微生物。 重组微生物过表达SNR84基因。 微生物是酵母。 酵母是酵母属（Saccharomyces sp。），Pachysolen sp。，Clavispora sp。，Kluyveromyces sp。，Debaryomyces sp。，Schwanniomyces sp。，Candida sp。，Pichia sp。或Dekkera sp。 重组微生物的保藏号KCTC 11388 BP为CEN.PK2-1D / pRS424-SNR84。

公开(公告)号：KR101657095B1

公开(公告)日：2016-09-19

申请号：KR1020100005576

申请日：2010-01-21

Applicant: 삼성전자주식회사 ,  고려대학교 산학협력단

Inventor： 조화영 ,  구현민 ,  박재찬 ,  박성민 ,  김경헌 ,  최인걸

IPC: C07K14/195 ,  C07K14/32 ,  C12P19/04 ,  C12N9/14

CPC classification number: C12P7/10 ,  C07K14/195 ,  C07K14/32 ,  C07K14/37 ,  C12N9/2437 ,  C12N9/248 ,  C12P19/02 ,  C12P19/14 ,  Y02E50/16 ,  Y02E50/343

Abstract: 셀룰라아제등 다당류가수분해효소의가수분해능을증진하고, 다당류에부착될수 있으며효소활성이결핍된단리된단백질에관한기술로서, 상기단리된단백질을첨가하는경우다당류또는그 가수분해물의분해를촉진할수 있어서다당류분해를위한가수분해효소사용량을줄일수 있다.

公开(公告)号：KR1020150051081A

公开(公告)日：2015-05-11

申请号：KR1020130132526

申请日：2013-11-01

Applicant: 삼성전자주식회사

Inventor： 이주영 ,  구현민 ,  박재찬 ,  김지은 ,  김진하 ,  박준성 ,  정순천 ,  조병관

IPC: C12N1/21 ,  C12N15/52 ,  C12P7/04

CPC classification number: C12P7/42 ,  C07K14/39 ,  C07K14/4705 ,  C12N9/1247 ,  C12P7/18 ,  C12P7/46 ,  C12P7/56 ,  Y02P20/52

Abstract: 클루베로마이세스마르시아누스에서산업적으로유용한형질을유도하기위해전사인자변이를이용한시스템및 이를이용하여제작한변형된클루베로마이세스마르시아누스를제공하기위한것이다. 이를위해유전자발현을조절하는전사인자복합체의조절인자에속하는각각의주요인자를이용하여변이라이브러리이를구축하였다. 구축된변이벡터라이브러리를균주에도입및 발현하여내산성이향상된변이주를개발하였다. 또한, 이를이용하여 3-HP를효율적으로생산할수 있는균주를개발하였고, 이러한균주는산업적으로매우유용하다.

Abstract translation: 本发明提供一种使用转录因子突变诱导马克斯克鲁维酵母的工业上有用的性状的系统，以及由其制备的改良的马克斯克鲁维酵母（Kluyveromyces marxianus）。 为此，通过使用属于调节基因表达的转录因子复合物的调节元件的各个关键因子构建了突变文库。 通过引入和表达在菌株中构建的突变体载体文库，开发了具有较高耐酸性的突变菌株。 此外，已经开发了能够有效地产生3-HP的菌株，并且该菌株在工业上非常有用。

公开(公告)号：KR1020140015086A

公开(公告)日：2014-02-06

申请号：KR1020120082815

申请日：2012-07-27

Applicant: 삼성전자주식회사

Inventor： 이규상 ,  이영민 ,  이우용 ,  구현민 ,  김진우 ,  박영경 ,  박진환 ,  조화영 ,  박재찬

IPC: C12N1/21 ,  C12N15/52 ,  C12N15/74 ,  C12P7/16

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N15/52 ,  C12N15/77 ,  C12Y101/01037 ,  C12Y101/05004 ,  Y02P20/52

Abstract: One embodiment of the present invention relates to a metabolism network model of Corynebacterium glutamicum for producing 1,4-BDO by Corynebacterium glutamicum, which is a strain that does not produce 1,4-BDO in a natural state. On the basis of the network model, metabolic properties of Corynebacterium glutamicum are analyzed. By predicting effects of a deletion target enzyme (ldhA, mqo or mdh) in a state in which Cat1, sucD, 4hbD, cat2 and adhE are included, and removing them, production efficiency of 1,4-BDO and increase of 1,4-BDO under the same fermentation conditions are confirmed. In addition, transformation microorganism produced by the method can produce 1,4-BDO in high efficiency, and therefore, can be effectively used in the industries.

Abstract translation: 本发明的一个实施方案涉及谷氨酸棒杆菌的代谢网络模型，用于通过谷氨酸棒杆菌生产1,4-BDO，其是在天然状态下不产生1,4-BDO的菌株。 在网络模型的基础上，分析谷氨酸棒杆菌的代谢特性。 通过预测在包含Cat1，sucD，4hbD，cat2和adhE的状态下缺失目标酶（ldhA，mqo或mdh）的作用并除去它们，1,4-BDO的生产效率和增加1,4 -BDO在相同的发酵条件下得到确认。 另外，通过该方法生产的转化微生物可以高效率地生产1,4-BDO，因此可以有效地用于工业中。