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公开(公告)号：KR1019930023466A
公开(公告)日：1993-12-18
申请号：KR1019920007972
申请日：1992-05-12
Applicant: 한국과학기술연구원
IPC: C12N1/20
Abstract: 본 발명은, 특히 그람 양성균과 기존의 항생물질 내성균주에 대해 강력한 항균활성을 갖는 다음의 구조식으로 표시되는 마크롤라이드계 항생물질 GERI-155와 그의 유도체를 제공하는 것이며, 이 GERI-155를 생산하는 균주인 스트렙토마이세스에스피. GER-155를 제공한다. 또한 본 발명은, 스트렙토마이세스에스피, GERI-155를 영양배지에서 배양하여서 GERI-155를 생산하는 방법을 제공하는 것이다.
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公开(公告)号：KR100011476B1
公开(公告)日：1982-02-26
申请号：KR1019800002095
申请日：1980-05-28
Applicant: 한국과학기술연구원
IPC: C07D498/08
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Patent Agency Ranking
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公开(公告)号：KR1020000056471A
公开(公告)日：2000-09-15
申请号：KR1019990005821
申请日：1999-02-22
Applicant: 한국과학기술연구원
IPC: C12N1/16
Abstract: PURPOSE: New strain of Candidanorvegica, CRM-9802 (KCTC 8900P) containing high concentration of ribonucleic acid (RNA) is provided which is used to manufacture a nucleotide spice from RNA of Candida cell extract. CONSTITUTION: Soil sample is mixed with sterile saline and vortexed. The supernatant is plated on YM agar and incubated at 30°C for 24-48 hours. The isolated colony is cultured and RNA content of each colony is measured. The colony showing high content of RNA is selected and its morphological, physiological and cultural characteristics are identified. The isolated yeast forms creamy egg shape colony on YM plate and does not make spores. The range of temperature for growth is from 15 to 35°C. Under anaerobic condition it metabolizes D-glucose and D-cellobiose as carbon sources. On the other hand, D-xylose, L-rhamnose, cellobios, salicin, arbutin, glycerol, D-glucitol, citrate and ethanol can be metabolized at aerobic conditions. Nitrate, nitrite, L-lysine and creatine can be used as a nitrogen source at aerobic conditions. The yeast is cultured in YM medium and harvested at 10 OD and RNA content of cell is measured by orcinol method. The RNA content of Candida norvegica is 12-13% of cell mass.
Abstract translation: 目的：提供含有高浓度核糖核酸（RNA）的新一代Candidanorvegica，CRM-9802（KCTC 8900P），用于从念珠菌细胞提取物的RNA制备核苷酸调味剂。 构成：将土壤样品与无菌盐水混合并涡旋。 将上清液接种在YM琼脂上，并在30℃下孵育24-48小时。 培养分离的菌落，测定每个菌落的RNA含量。 选择显示高含量RNA的菌落，确定其形态，生理和文化特征。 分离的酵母在YM板上形成奶油蛋形菌落，不产生孢子。 生长温度范围为15〜35℃。 在厌氧条件下，将D-葡萄糖和D-纤维二糖代谢为碳源。 另一方面，D-木糖，L-鼠李糖，纤维二糖，水杨蛋白，熊果苷，甘油，D-葡萄糖醇，柠檬酸盐和乙醇可以在需氧条件下代谢。 硝酸盐，亚硝酸盐，L-赖氨酸和肌酸可用作有氧条件下的氮源。 将酵母在YM培养基中培养并在10℃下收获，并通过α-淀粉酶法测定细胞的RNA含量。 假丝酵母的RNA含量为细胞质量的12-13％。
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19.发明公开신규 락토바실러스 속 (LACTOBACILLUS SP.)MT-1077 및 그로부터 생산되는 신규 박테리오신 失效新颖的LACTOBACILLUS SP。 MT-1077（KCTC 8903P）和新生成的细菌
公开(公告)号：KR1020000047065A
公开(公告)日：2000-07-25
申请号：KR1019980063818
申请日：1998-12-31
Applicant: 한국과학기술연구원
Abstract: PURPOSE: A novel lactobacillus sp. MT-1077 (KCTC 8903P) and a novel bacteriocin produced therefrom are provided. The bacteriocin has stability against broad range of pH, heat stability and antifungal activity and used as food preservation agent, biological fermentation regulator, and antifungal agent. CONSTITUTION: A lactobacillus MT-1077 is isolated from Kimchi. The method for purifying bacteriocin from lactobacillus MT-1077 comprises the steps of: incubating lactobacillus MT-1077 in MRS broth; precipitating the lactobacillus MT-1077 fermented culture broth by addition of ammonium hydrogen sulfate; passing the protein precipitates through the column packed with carboxymethyl sepharose CL-6B and eluting the adsorbed protein with 0.1M sodium chloride; passing the elutes through the column packed with phenyl sepharose CL-4B and eluting the adsorbed protein with 10% ammonium hydrogen sulfate solution; and purifying the elutes by HPLC
Abstract translation: 目的：一种新型乳酸杆菌 提供MT-1077（KCTC 8903P）和由其制备的新型细菌素。 细菌素具有广泛的pH，热稳定性和抗真菌活性的稳定性，并且用作食品保存剂，生物发酵调节剂和抗真菌剂。 构成：从泡菜中分离出一种乳杆菌MT-1077。 用于从MT-1077乳杆菌中纯化细菌素的方法包括以下步骤：在MRS肉汤中培养MT-1077乳杆菌; 通过加入硫酸氢铵沉淀出乳杆菌MT-1077发酵培养液; 使蛋白沉淀物通过填充有羧甲基sepharose CL-6B的柱并用0.1M氯化钠洗脱吸附的蛋白质; 将洗脱液通过填充有苯基sepharose CL-4B的柱并用10％硫酸氢铵溶液洗脱吸附的蛋白质; 并通过HPLC纯化洗脱液
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20.发明公开신균주 스트렙토마이세스속 MT1882-V(KCTC 8800P)와 피에리시딘 A1의 인터루킨-2생성 억제제로서의 용도 失效使用新型菌株链霉菌属MT1882-V（KCTC 8800P）和Pierceine A1作为白细胞介素-2产生的抑制剂
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