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公开(公告)号：KR100137802B1
公开(公告)日：1998-04-30
申请号：KR1019950006027
申请日：1995-03-22
Applicant: 한국과학기술연구원 , 대한알콜산업기술연구조합
CPC classification number: Y02E50/17
Abstract: 에탄올 발효시 회수된 균체를 아황산염 공급원으로 처리함으로써 효모 성장과 에탄올 수율을 감소시키는 오염균을 제어할 수 있다. 아황산염 공급원은 이에 대한 효모 및 오염균의 내장 차이에 기초하여 적어도 100pm의 염 농도로 호기적 조건하에 사용되며, 과산화수소를 일정 농도 이하로 가함으로써 오염균 제어 효과를 더욱 개선시킬 수도 있다.
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公开(公告)号：KR1020050028539A
公开(公告)日：2005-03-23
申请号：KR1020030064824
申请日：2003-09-18
Applicant: 한국과학기술연구원
IPC: C12Q1/68
Abstract: A detection method of E. coli in water samples using PCR is provided, thereby detecting infection of E. coli in drinking water at an early stage using a small amount of DNA of E. coli present in a sample, so that a seed culturing process can be eliminated. The detection method of E. coli in water samples comprises performing PCR using a primer for a flagellum forming gene hdfR, wherein the primer comprises a primer set consisting of a sense primer LysR-p1 having the nucleotide sequence of SEQ ID NO:1, AATCTAGTTGCTGTGCCAGT, and an antisense primer LysR-p2 having the nucleotide sequence of SEQ ID NO:2, TTGCTGGGATATTTCACTTT.
Abstract translation: 提供使用PCR的水样品中的大肠杆菌的检测方法，从而利用样品中存在的少量大肠杆菌DNA，早期检测饮用水中大肠杆菌的感染，使种子培养过程 可以消除。 水样中大肠杆菌的检测方法包括使用鞭毛形成基因hdfR的引物进行PCR，其中引物包含由具有SEQ ID NO：1的核苷酸序列的有义引物LysR-p1，AATCTAGTTGCTGTGCCAGT ，以及具有SEQ ID NO：2的核苷酸序列的TTGCTGGGATATTTCACTTT的反义引物LysR-p2。
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公开(公告)号：KR100468547B1
公开(公告)日：2005-01-29
申请号：KR1020020045165
申请日：2002-07-31
Applicant: 한국과학기술연구원
IPC: C12N15/55
Abstract: PURPOSE: Provided are a gene coding lipase of Acinetobacter species SY-01 with stereoselective substrate specificity and a protein expressed therefrom. CONSTITUTION: A gene is characterized by coding amino acid sequence of lipase of Acinetobacter species SY-01, represented by the SEQ ID NO:2. A transformed Bacillus subtilis 168/pSYLipSM(1) (KCTC 10267BP) is obtained by transformation with a recombinant vector plasmid pSYLipSM(1) containing the gene coding the lipase of Acinetobacter species SY-01. The lipase of Acinetobacter species SY-01 is manufactured by the steps of culturing the transformed bacillus subtilis, and isolating and purifying expressed lipase of Acinetobacter species SY-01 from the cultured cell.
Abstract translation: 目的：提供具有立体选择性底物特异性的不动杆菌种SY-01的编码脂肪酶的基因和由其表达的蛋白质。 组成：基因的特征在于编码不动杆菌属物种SY-01的脂肪酶的氨基酸序列，由SEQ ID NO：2表示。 通过用含有编码不动杆菌属物种SY-01的脂肪酶的基因的重组载体质粒pSYLipSM（1）转化获得转化的枯草芽孢杆菌168 / pSYLipSM（1）（KCTC 10267BP）。 不动杆菌菌种SY-01的脂肪酶是通过培养转化的枯草芽孢杆菌，从培养细胞中分离纯化不动杆菌菌种SY-01的表达脂肪酶。
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公开(公告)号：KR1020020026684A
公开(公告)日：2002-04-12
申请号：KR1020000057916
申请日：2000-10-02
Applicant: 한국과학기술연구원
IPC: C12P41/00
Abstract: PURPOSE: Provided is an effective resolution method of chiral compounds containing hydroxy group or ester group, by using lipase produced by Burkholderia Cocovenenans SY-01I(KFCC 11111). CONSTITUTION: Chiral compounds(formula 1, 2, 3 4 and 5) containing hydroxy group or ester group are resolved by hydrolyzing and trans-esterifying using the solid lipase prepared by dialysis or lyophilizing, wherein the hydrolysis is carried out at 5-35deg.C with by phosphate buffer solution of pH7.09; and the trans-esterification is conducted at 0-55 deg.C with organic solvent and an acylating agent. The organic solvent is selected from n-hexane, ethylacetate, benzene, toluene, THF, diethylether, acetonitrile, dichloroethane, dichloromethane, 1,4-dioxane, methanol, DMSO, DMF and hexane/ethylacetate(9/1 -5/5) and the above acylating agent is chosen from vinylacetate, isopropenylacetate, anhydrous acetic acid, anhydrous propionic acid and anhydrous butyric acid.
Abstract translation: 目的：通过使用伯克霍尔德氏菌SY-01I（KFCC 11111）生产的脂肪酶，提供含有羟基或酯基的手性化合物的有效解决方法。 构成：使用通过透析或冻干制备的固体脂肪酶进行水解和酯交换，分解含有羟基或酯基的手性化合物（式1,2,3,4,5），其中水解进行5-35度。 C用磷酸盐缓冲溶液pH7.09; 并且酯交换在0-55℃下与有机溶剂和酰化剂进行。 有机溶剂选自正己烷，乙酸乙酯，苯，甲苯，THF，二乙醚，乙腈，二氯乙烷，二氯甲烷，1,4-二恶烷，甲醇，DMSO，DMF和己烷/乙酸乙酯（9/1 -5/5） 并且上述酰化剂选自乙酸乙烯酯，异丙烯基乙酸酯，无水乙酸，无水丙酸和无水丁酸。
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公开(公告)号：KR1020020017066A
公开(公告)日：2002-03-07
申请号：KR1020000050149
申请日：2000-08-28
Applicant: 한국과학기술연구원
IPC: C12N1/20
Abstract: PURPOSE: Provided is a microorganism strain producing thermophile lipase. The above strain is obtained from the soil samples near the industrial complex, identified as a novel lipase producing strain different from known Burkholderia cepacia and named as SY-02(Burkholderia cepacia SY-02). It shows thermo-stability and high enzyme activity at the higher temperature than the melting point of fat and is thus widely useful as a lipase producing strain in the field of industry. CONSTITUTION: The strain Burkholderia cepacis SY-02(KFCC-111210) produces thermophilic lipase which is secreted into the exterior of a cell of Burkholderia cepacia SY-02, and shows activity at 40-80 deg.C, preferably 70 deg.C.
Abstract translation: 目的：提供一种生产嗜热脂肪酶的微生物菌株。 上述菌株从工业化合物附近的土壤样品中获得，鉴定为与已知的洋葱伯克霍尔德菌（Burkholderia cepacia）不同的新型脂肪酶生产菌株，命名为SY-02（洋葱伯克霍尔德菌（SYK））。 它在比脂肪的熔点高的温度下显示出热稳定性和高酶活性，因此在工业领域中广泛用作脂肪酶生产菌株。 构成：洋葱伯克霍尔德菌SY-02（KFCC-111210）产生嗜热脂肪酶，其分泌到洋葱伯克霍尔德菌SY-02细胞的外部，并在40-80℃，优选70℃显示出活性。
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Patent Agency Ranking
公开(公告)号：KR1020010109632A
公开(公告)日：2001-12-12
申请号：KR1020000029739
申请日：2000-05-31
Applicant: 한국과학기술연구원 , 한올바이오파마주식회사 , 경동제약 주식회사
IPC: C12N9/16
Abstract: 본발명은 신균주 벌콜데리아 코코베네난스(Burkholderia Cocovenenans) SY-01과 이로부터 생산되는 입체선택적 기질특이성을 갖는 리파제에 관한 것으로, 더욱 상세하게는 항진균제의 원료로 사용되는 이트라코나졸의 중간체로서 라세믹 혼합물인 시스-2-(브로모메틸)-2-(2,4-디클로로 페닐)-1,3-디옥소란-4-메틸 아세테이트의 에스테르결합을 입체선택적으로 분해할 수 있는 리파제에 관한 것이다.
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公开(公告)号：KR1019960034397A
公开(公告)日：1996-10-22
申请号：KR1019950006027
申请日：1995-03-22
Applicant: 한국과학기술연구원 , 대한알콜산업기술연구조합
Abstract: 에탄올 발효시 회수된 균체를 아황산염 공급원으로 처리함으로써 효모 성장과 에탄올 수율을 감소시키는 오염균을 제어할 수 있다. 아황산염 공급원은 이에 대한 효모 및 오염균의 내성 차이에 기초하여 적어도 100ppm의 염 농도로 호기적 조건하에 사용되며, 과산화수소를 일정 농도 이하로 가함으로써 오염균 제어 효과를 더욱 개선시킬 수 있다.
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公开(公告)号：KR100553336B1
公开(公告)日：2006-02-20
申请号：KR1020030064824
申请日：2003-09-18
Applicant: 한국과학기술연구원
IPC: C12Q1/68
Abstract: 본 발명은 병원성 및 비병원성 대장균에 공통으로 존재하는 편모형성유전자 hdfR에 대한 프라이머를 신규 제작하고, 이 프라이머를 이용한 중합효소연쇄반응(PCR) 기법으로 식수에서 대장균의 오염 여부를 조기에 확인할 수 있는 대장균 검출 방법에 관한 것이다. 본 발명에 따른 대장균 검출 방법으로 인하여 시료에 소량의 대장균이 존재하여도 증균 배양 단계를 거치지 않고 단지 소량의 DNA만을 가지고도 신속하고 정확하게 바로 대장균을 검출할 수 있다.
대장균, hdfR 유전자-
公开(公告)号：KR100411236B1
公开(公告)日：2003-12-18
申请号：KR1020000050149
申请日：2000-08-28
Applicant: 한국과학기술연구원
IPC: C12N1/20
Abstract: PURPOSE: Provided is a microorganism strain producing thermophile lipase. The above strain is obtained from the soil samples near the industrial complex, identified as a novel lipase producing strain different from known Burkholderia cepacia and named as SY-02(Burkholderia cepacia SY-02). It shows thermo-stability and high enzyme activity at the higher temperature than the melting point of fat and is thus widely useful as a lipase producing strain in the field of industry. CONSTITUTION: The strain Burkholderia cepacis SY-02(KFCC-111210) produces thermophilic lipase which is secreted into the exterior of a cell of Burkholderia cepacia SY-02, and shows activity at 40-80 deg.C, preferably 70 deg.C.
Abstract translation: 目的：提供一种产生嗜热脂肪酶的微生物菌株。 上述菌株从工业园区附近的土壤样品中获得，鉴定为与已知洋葱伯克霍尔德菌不同的新型脂肪酶生产菌株，命名为SY-02（洋葱伯克霍尔德菌SY-02）。 它在高于脂肪熔点的温度下显示热稳定性和高酶活性，因此广泛用作工业领域中的脂肪酶生产菌株。 构成：菌株伯克霍尔德氏菌SY-02（KFCC-111210）产生分泌到洋葱伯克霍尔德氏菌SY-02细胞外部的嗜热性脂肪酶，并在40-80℃，优选70℃显示出活性。
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公开(公告)号：KR1020020065130A
公开(公告)日：2002-08-13
申请号：KR1020010005488
申请日：2001-02-05
Applicant: 한국과학기술연구원
IPC: B01D39/14
CPC classification number: B01D39/14 , B01D39/2055 , B01D2239/04 , B01D2239/10
Abstract: PURPOSE: A lateral surface filtering type biofilter is provided in which polluted air is introduced into in a direction of lateral surface of the biofilter to maximize an inflow cross-sectional area of the polluted air, and a purification method of polluted air using an electric biofilter is provided. CONSTITUTION: The lateral surface filtering type biofilter comprises a cylindrical housing part(3) equipped with upper and lower surfaces at the center of which a vent(1) is positioned, wherein an inlet(2) is formed on the lateral surface of the housing part; and a filter part(7) formed in a doughnut type cylinder shape which is positioned inside the housing part, in which a filter filling material is filled in a space between a concentric circle shaped net type outer wall(4) and a net type inner wall(5), and a cavity of the center of which is connected to the vent(1) of the housing part, wherein the lateral surface filtering type biofilter further comprises an injection pipe(6) which is positioned at the upper part of the housing part(3) to supply moisture and nutritive elements to the filter filling material, and a flow meter(8) which is attached to the vent of the housing part to measure the amount of air treated, the filter filling material comprises activated carbon capable of adsorbing polluted air outstandingly, compost used as a culture medium of microorganisms, sawdust and microorganism source, and the microorganism source is a supernatant of a culture fluid which is prepared by cultivating activated sludge obtained from a sewage disposal plant or a supernatant of castings of an earthworm. The method for purifying polluted air using the lateral surface filtering type biofilter comprises the steps of flowing the polluted air into the inlet(2) of the lateral surface filtering type biofilter, and exhausting the purified air through the vent.
Abstract translation: 目的：提供一种侧面过滤型生物过滤器，其中污染空气沿生物过滤器侧表面的方向被引入，以最大化污染空气的流入横截面面积，并使用电生物过滤器净化污染空气的方法 被提供。 构成：侧表面过滤型生物过滤器包括一个圆柱形的壳体部分（3），其中装有上表面和下表面，其中心具有排气口（1），其中入口（2）形成在壳体的侧表面上 部分; 以及形成为环状圆筒状的过滤器部（7），其位于所述壳体部内部，所述过滤器填充材料填充在同心圆形网状外壁（4）和网状内侧 壁（5），并且其中心的空腔连接到所述壳体部分的通风口（1），其中所述侧表面过滤型生物过滤器还包括注入管（6），所述注入管位于所述壳体部分的上部 壳体部分（3）向过滤器填充材料供应水分和营养元素;以及流量计（8），其连接到壳体部分的通风口以测量经处理的空气量，过滤器填充材料包括活性炭 吸附污染空气的堆肥，作为微生物，锯屑和微生物来源的培养基的堆肥，微生物源是通过培养从污水中得到的活性污泥制备的培养液的上清液 施用植物或蚯蚓铸件的上清液。 使用侧面过滤型生物过滤器净化污染空气的方法包括将污染空气流入侧面过滤型生物过滤器的入口（2）并通过排气口排出净化空气的步骤。
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