Abstract:
A novel antibacterial peptide is provided to show strong antibacterial activity on a wider range of microorganism species compared with a conventional antibacterial peptide and have less or no toxicity than the conventional antibacterial peptide. A peptide having the antibacterial activity in a highly concentrated salt is represented by a formula(1) of N-terminal-A P K A M X^1 X^2 X^3 X^4 X^5 L Q K K G I-C-terminal, wherein A is alanine; P is proline; K is lysine; M is methionine; X^1 is one basic amino acid; X^2 is two hydrophobic amino acids selected from the group consisting of alanine, valine and leucine; X^3 is two basic amino acids; X^4 is two hydrophobic amino acids selected from the group consisting of alanine, valine and leucine; X^5 is one basic amino acid; L is leucine; Q is glutamine; G is glycine; and I is iso-leucine. An antibacterial composition comprises the peptide as an effective ingredient.
Abstract translation:提供了一种新型抗菌肽,与常规抗菌肽相比,在更广泛的微生物种类上显示出强的抗菌活性,并且具有比常规抗菌肽更少或没有毒性。 在高浓度盐中具有抗菌活性的肽由N末端-A P K A M X 1 X X 2 X 3 3 X 4 X 5 L Q K K G I-C-末端的式(1)表示,其中A是丙氨酸; P是脯氨酸; K为赖氨酸; M是甲硫氨酸; X ^ 1是一种碱性氨基酸; X ^ 2是选自丙氨酸,缬氨酸和亮氨酸的两种疏水性氨基酸; X ^ 3是两种碱性氨基酸; X ^ 4是选自丙氨酸,缬氨酸和亮氨酸的两种疏水性氨基酸; X ^ 5是一种碱性氨基酸; L是亮氨酸; Q是谷氨酰胺; G是甘氨酸; 我是异亮氨酸。 抗菌组合物包含该肽作为有效成分。
Abstract:
PURPOSE: A transposon for bidirectional intramolecular genome deletions, a construction of a microorganism mutant and the identification of nonessential genes using the same transposon are provided, thereby effectively constructing the nonessential gene-removed Escherichia coli mutant. CONSTITUTION: Transposon TnRIBD sequentially comprises Tn5 transposase recognition site having the nucleotide sequence of SEQ ID NO: 2; tetR gene having the nucleotide sequence of SEQ ID NO: 4; Tnγδ transposase recognition site having the nucleotide sequence of SEQ ID NO: 3; CmR gene having the nucleotide sequence of SEQ ID NO: 5; Tnγδ transposase recognition site having the reverse complementary nucleotide sequence with SEQ ID NO: 3; sacB gene having the nucleotide sequence of SEQ ID NO: 6; and Tn5 transposase recognition site having the reverse complementary nucleotide sequence with SEQ ID NO: 2 in a direction of 5' to 3'. A construction method of a microorganism mutant comprises the steps of: constructing the transposon comprising the transposase recognition site and a selection marker; inserting the transposon into a certain site in the genome of a microorganism; and cloning a transposase expression vector into the genome of the microorganism to remove a portion of chromosome in both ends.
Abstract translation:目的:提供用于双向分子内基因组缺失的转座子,构建微生物突变体和使用相同转座子鉴定非必需基因,从而有效构建非必需基因去除的大肠杆菌突变体。 构成:转座子TnRIBD依次包含具有SEQ ID NO:2的核苷酸序列的Tn5转座酶识别位点; 具有SEQ ID NO:4的核苷酸序列的tetR基因; 具有SEQ ID NO:3的核苷酸序列的Tnγδ转座酶识别位点; 具有SEQ ID NO:5的核苷酸序列的CmR基因; 具有与SEQ ID NO:3相反的互补核苷酸序列的Tnγδ转座酶识别位点; 具有SEQ ID NO:6的核苷酸序列的sacB基因; 和具有与SEQ ID NO:2相反的互补核苷酸序列的5'至3'的Tn5转座酶识别位点。 微生物突变体的构建方法包括以下步骤:构建包含转座酶识别位点和选择标记的转座子; 将转座子插入微生物基因组中的某个位点; 并将转座酶表达载体克隆到微生物的基因组中以去除两端染色体的一部分。
Abstract:
본 발명은 염색체의 특정부위가 제거된 미생물 변이주의 제조방법에 관한 것으로, 더욱 상세하게는, sacB 유전자, I-sceI 제한효소 절제부위(restriction site), 선별마커(selectable marker) 및 미생물 염색체의 일부와 상동인 상동부위(homology arm)를 포함하는 선형 DNA 단편 및 이를 이용한 부위 특이적 동성 재조합(site specific homologous-recombination)에 의하여, 선별마커의 남김 없이 염색체의 특정부위를 제거함으로써, 새로운 미생물 변이주를 제조하는 방법에 관한 것이다. 또한, 본 발명에 따른 방법은 상기와 같은 방법을 반복하여 순차적으로 특정 유전자들을 제거하는 단계를 추가적으로 포함할 수 있다.
Abstract:
본 발명에 따른 디지털 비행촬영장치는, 렌즈(18)의 방향변환이 가능한 디지털 카메라가 설치되며 공중부양이 가능한 촬영로봇(10); 및 촬영로봇(10)의 공중부양을 원격제어하기 위한 공중부양 제어장치(22)와, 카메라 렌즈(18)의 방향 및 줌을 조절할 수 있는 렌즈조절장치(24, 26)와, 카메라의 뷰파인더(28)와, 카메라의 셔터버튼(26)이 설치되는 리모콘(20);을 구비하는 것을 특징으로 한다. 본 발명에 의하면, 원거리에서 피사체를 확인하면서 카메라의 촬영높이 및 촬영각도를 조절할 수 있기 때문에 혼자 여행을 하는 경우에도 누군가가 찍어주는 느낌과 똑같은 매우 자연스러운 느낌의 셀프 카메라 사진을 얻을 수 있게 된다. 또한, 위험한 곳의 사진을 원격적으로 찍는 등 레저용, 산업용, 및 군사용 등으로도 사용이 가능하다.
Abstract:
PURPOSE: A linear DNA fragment for developing a mutated microorganism of which specific region in chromosome is removed and a method for preparing the same microorganism are provided, thereby completely removing selection marker and foreign DNA from the chromosome. CONSTITUTION: A linear DNA fragment for developing a mutated microorganism of which specific region in chromosome is removed comprises 500 bp homologous domains A and C which is homologous to the both ends of the chromosome to be removed and related to the lambda-red recombination when this linear DNA fragment is inserted into the both ends of microorganism; 500 bp homologous domain B which is homologous to a region adjacent to one end of the chromosome to be removed and related to the homologous recombination when a selection marker at one end is removed; sacB gene having the nucleotide sequence set forth in SEQ ID NO: 6 as a factor for confirming the selection marker and selection marker removement; and the restriction enzyme I-SceI recognition site having the nucleotide sequence set forth in SEQ ID NO: 4 related to the homologous recombination for removing the selection marker.
Abstract translation:目的:提供用于开发除去染色体中特定区域的突变微生物的线性DNA片段和制备相同微生物的方法,从而从染色体中完全去除选择标记和外来DNA。 构成:用于开发其中去除染色体中特定区域的突变微生物的线性DNA片段包含500bp同源结构域A和C,其与要去除的染色体的两端同源且与λ-红色重组有关 将线性DNA片段插入微生物的两端; 500bp同源结构域B,其与待去除的染色体的一端相邻的区域同源,并且在一端的选择标记被去除时与同源重组相关; 具有SEQ ID NO:6所示核苷酸序列的sacB基因作为确认选择标记和选择标记移除的因子; 以及具有SEQ ID NO:4所示核苷酸序列的限制酶I-SceI识别位点与用于除去选择标记的同源重组相关。