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公开(公告)号：KR101254120B1
公开(公告)日：2013-04-12
申请号：KR1020110039243
申请日：2011-04-26
Applicant: 한국과학기술원
Abstract: 본 발명은 해조류 유래의 갈락탄을 이용한 5-하이드록시메틸 플루프랄 및 갈락토오즈의 생산방법에 관한 것으로, 보다 상세하게는 막분리 공정을 이용한 고수율의 5-하이드록시메틸 플루프랄 및 갈락토오즈의 생산방법으로, 상기 본 발명에 따른 생산방법은 값싼 해조류의 이용 뿐 아니라 제조공정에 있어서도 기존 방법에 비해 훨씬 경제적이고 효율적인 장점이 있으며, 갈락탄의 가수분해물로부터 나노여과 막분리 공정과 전기투석 막분리 공정을 순차적으로 적용함으로써 5-하이드록시메틸 플루프랄 뿐 아니라 이의 부산물인 갈락토오즈를 고효율로 수득할 수 있는 장점이 있다.
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公开(公告)号：KR1020120121323A
公开(公告)日：2012-11-05
申请号：KR1020110039243
申请日：2011-04-26
Applicant: 한국과학기술원
CPC classification number: C07H3/02 , C07D307/50 , C07H1/00
Abstract: PURPOSE: A method for preparing 5-hydroxymethyl furfural and galactose using algal galactans is provided to obtain the compounds of high efficiency. CONSTITUTION: A method for preparing galactose from algal galactans comprises: a step of hydrolyzing galactans extracted from red algae under the presence of an acid catalyst; a step of obtaining a reactant from hydrolysate; and a step of collecting galatose from the reactant by nanofiltration. The galactan is agar, carrageenan, or a mixture thereof. The acid catalyst is hydrochloric acid(HCl), sulfuric acid(H_2SO_4), trifluoroacetic acid(TFA), phosphoric acid(H_3PO_4), or hydroiodic acid(HI). [Reference numerals] (AA, HH) Galactan; (BB, II) Acid catalyst; (CC, JJ) Acid decomposition; (DD, KK) Neutralization; (EE, LL) Nanofiltration; (FF, OO) Basic solution; (GG, PP) Galactose; (MM) Electrodialysis; (NN) Salt; (QQ) S-HMF
Abstract translation: 目的：提供使用藻类半乳聚糖制备5-羟甲基糠醛和半乳糖的方法，以获得高效率的化合物。 构成：从藻类半乳聚糖制备半乳糖的方法包括：在酸催化剂存在下水解从红藻中提取的半乳聚糖的步骤; 从水解产物获得反应物的步骤; 以及通过纳米过滤从反应物中收集半乳糖的步骤。 半乳聚糖是琼脂，角叉菜胶或其混合物。 酸催化剂是盐酸（HCl），硫酸（H 2 SO 4），三氟乙酸（TFA），磷酸（H 3 PO 4）或氢碘酸（HI）。 （标号）（AA，HH）半乳聚糖; （BB，II）酸催化剂; （CC，JJ）酸分解; （DD，KK）中和; （EE，LL）纳滤; （FF，OO）基本解决方案; （GG，PP）半乳糖; （MM）电渗析; （NN）盐; （QQ）S-HMF
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公开(公告)号：KR100447529B1
公开(公告)日：2004-09-08
申请号：KR1020010066772
申请日：2001-10-29
Applicant: 한국과학기술원
IPC: C10G32/00
Abstract: PURPOSE: A method for recovering a biocatalyst and desulfurization oil from the emulsion generated during the biological petroleum desulfurization process is provided, to recover the desulfurized oil to the amount of 95 % or more without deterioration of the desulfurization performance. CONSTITUTION: A biocatalyst and desulfurization oil are recovered from the emulsion generated during the biological petroleum desulfurization process, by adding an alcohol to the three-phase emulsion of oil-biocatalyst-water and stirring the mixture; and separating the pretreated emulsion into three layers of an oil, a biocatalyst and water. Preferably the biocatalyst is Gordonia nitida CYKS 1 (KCTC0431BP). Also a biocatalyst and desulfurization diesel oil are recovered by adding ethanol to the three-phase emulsion of diesel oil-Gordonia nitida CYKS 1-water to the concentration of 1-4 % (v/v) and stirring the mixture; and separating the pretreated emulsion into three layers of a diesel oil, a Gordonia nitida CYKS 1 biocatalyst and water.
Abstract translation: 目的：提供一种从生物石油脱硫过程中产生的乳液中回收生物催化剂和脱硫油的方法，以在不使脱硫性能劣化的情况下将脱硫油回收到95％或更多的量。 构成：生物催化剂和脱硫油从生物石油脱硫过程中产生的乳液中回收，向油 - 生物催化剂 - 水的三相乳液中加入醇并搅拌混合物; 并将预处理的乳液分成三层油，生物催化剂和水。 生物催化剂优选为Gordonia nitida CYKS1（KCTC0431BP）。 通过向柴油Gordonia nitida CYKS 1-水的三相乳液中加入乙醇至1-4％（v / v）的浓度并搅拌混合物来回收生物催化剂和脱硫柴油; 并将经预处理的乳液分离成三层柴油，Gordonia nitida CYKS 1生物催化剂和水。
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公开(公告)号：KR1020010009719A
公开(公告)日：2001-02-05
申请号：KR1019990028251
申请日：1999-07-13
Applicant: 한국과학기술원
IPC: C12P7/62
Abstract: PURPOSE: A method for separating polyhydroxy-alkanoate from a microorganism is provided, thereby the installation cost and operating cost can be lowered, and polyhydroxy-alkanoate is simply separated in higher yield and purity, from a microorganism containing polyhydroxy-alkanoate in its body, with generating a little amount of liquid waste. CONSTITUTION: The method for separating polyhydroxy-alkanoate from a microorganism comprises the steps of: dissolving the cell components of a microorganism by adding SDS into the microorganism fermented culture containing polyhydroxy-alkanoate, and heating the solution with agitation to 50 to 150deg.C; recovering polyhydroxy-alkanoate from the treated solution; washing the recovered polyhydroxy-alkanoate with solvent; and drying the washed polyhydroxy-alkanoate, in which microorganism is selected from Alcaligenes latus, Alcaligenes eutrophus, Azotobacter vinelandii, Escherichia coli, Pseudomonas spp., and Methylotrophs, and SDS is added in an amount of 0.3 to 0.5g per 1g of dried microorganism containing polyhydroxy-alkanoate.
Abstract translation: 目的：提供从微生物中分离多羟基 - 链烷酸酯的方法，从而降低了安装成本和操作成本，并且从其在其体内含有聚羟基 - 链烷酸酯的微生物中以更高的产率和纯度简单地分离出多羟基 - 链烷酸酯， 产生少量的废液。 构成：从微生物分离多羟基链烷酸酯的方法包括以下步骤：通过将SDS加入到含有多羟基 - 链烷酸酯的微生物发酵培养基中，并将该溶液搅拌加热至50〜150℃，从而溶解微生物的细胞成分; 从处理的溶液中回收多羟基 - 链烷酸酯; 用溶剂洗涤回收的聚羟基链烷酸酯; 并干燥洗涤过的多羟基 - 链烷酸酯，其中微生物选自产碱杆菌，产碱杆菌，维生素Azotobacter vinelandii，大肠杆菌，假单胞菌属和甲状腺营养素，SDS以0.3至0.5g / 1g干燥微生物 含有多羟基 - 链烷酸酯。
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公开(公告)号：KR1020000066159A
公开(公告)日：2000-11-15
申请号：KR1019990013041
申请日：1999-04-13
Applicant: 한국과학기술원
IPC: C12M1/00
CPC classification number: C12P7/46 , C12M23/58 , C12M29/18 , C12M41/26 , C12P7/40 , C12P7/42 , C12P7/48
Abstract: PURPOSE: A process for efficiently producing organic acid by continuously fermenting organic acid fermentation microorganisms in a cell recirculation multistage continuous fermenter and recovering organic acid from the culture solution is provided which reduces equipment and production costs and promotes productivity. CONSTITUTION: An organic acid-fermentable microorganism is fermented in a cell recirculation multistage continuous fermenter connected in series a fermenter comprising a fermentation tank, a temperature controller, an agitator and a pH controller, a circulation pump for circulating fermentation mediums from the fermenter to the outside and a cell separator for separating the fermentation mediums and recirculating. The process can be applied to a fermentation process for producing lactic acid, acetic acid, formic acid, citric acid, malic acid, maleic acid, fumaric acid or succinic acid that shows productivity reduction by final products.
Abstract translation: 目的：提供一种通过在细胞再循环多级连续发酵罐中连续发酵有机酸发酵微生物并从培养液中回收有机酸有效生产有机酸的方法，其降低了设备和生产成本并提高了生产率。 构成：将有机酸可发酵微生物在连续发酵的细胞再循环多级连续发酵罐中发酵，发酵罐包括发酵罐，温度控制器，搅拌器和pH控制器，循环泵，用于将发酵培养基从发酵罐循环到 外部和用于分离发酵培养基并再循环的细胞分离器。 该方法可用于产生乳酸，乙酸，甲酸，柠檬酸，苹果酸，马来酸，富马酸或琥珀酸的发酵方法，其显示最终产物的生产率降低。
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Patent Agency Ranking
公开(公告)号：KR100253914B1
公开(公告)日：2000-04-15
申请号：KR1019970071325
申请日：1997-12-20
Applicant: 한국과학기술원
IPC: G01N21/07
CPC classification number: G01N21/05
Abstract: PURPOSE: A closed type online measuring apparatus is provided to measure on-line without diluting a sample at thick density by adjusting the length of a light transmittance passage. CONSTITUTION: A cuvette holder comprises a substrate; a fixing plate vertically formed on the substrate for transmitting radiant light and circulating a sample; a moving plate vertically installed on the substrate to move axially on the substrate and to transmit the radiant light; a metallic rod connecting an electric fixing plate and the moving plate; and an isolating membrane having a closed space between the electric fixing plate and the moving plate. The cuvette holder has a micrometer to transfer the moving plate finely, and measures the density of material in a reactor throughout on-line at low costs without reactive solution loss, contamination and error for automatic process control and optimal condition.
Abstract translation: 目的：提供一种闭式在线测量装置，通过调节光线透过率通道的长度，在线测量而不用浓密度稀释样品。 构成：比色皿支架包括基板; 垂直形成在所述基板上用于透射辐射光并使样品循环的固定板; 垂直安装在基板上的移动板，在基板上轴向移动并透射辐射光; 连接电气固定板和移动板的金属杆; 以及在电气固定板和移动板之间具有封闭空间的隔离膜。 比色皿支架具有微米，以精细地移动移动板，并且以低成本在线检测反应堆中的材料的密度，而没有反应溶液损失，自动过程控制和最佳条件下的污染和误差。
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公开(公告)号：KR1020170141466A
公开(公告)日：2017-12-26
申请号：KR1020160074548
申请日：2016-06-15
Applicant: 한국과학기술원 , 재단법인 탄소순환형 차세대 바이오매스 생산전환 기술연구단
Abstract: 본발명은비닐기, 아크릴기또는메타아크릴기를포함하는친핵체단량체와비닐기, 아크릴기또는메타아크릴기및 알킬할라이드작용기를포함하는친전자체단량체및 개시제를기상으로라디칼중합함과동시에기판상에코팅하는것을특징으로하는이온가교된고분자가코팅된수처리용분리막의제조방법에관한것으로서, 후처리없이도매우높은효율과투과도의물과기름분리성능을가지는이온가교된고분자가코팅된분리막을단지 1단계의공정으로쉽게제조하는것이가능하여비용절감효과가있다.
Abstract translation: 本发明是乙烯基基团，丙烯酸基团或含基团和乙烯基基团，丙烯酸基团或甲基丙烯酸基团和亲电基团的聚合自身的单体和含烷基卤官能团的气相引发剂的甲基丙烯酸亲核体的单体，并在同一时间的涂布在基板上 离子交联的聚合物涉及用于涂覆的水处理分离膜的制造方法中，其次是非常高的效率，并分离具有的性能仅离子交联聚合物涂覆的膜的步骤1，而无需其特征在于，油和水的传输速率 可以通过图3的过程容易地制造它。
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公开(公告)号：KR1020140048516A
公开(公告)日：2014-04-24
申请号：KR1020120114555
申请日：2012-10-16
Applicant: 에스케이이노베이션 주식회사 , 한국과학기술원
Abstract: The present invention relates to a novel beta-agarase gene and a producing method of a beta-agarase using the same. The beta-agarase gene according to the present invention has advantages of directly accumulating a beta-agarase at high concentration when introducing double strains which cannot produce a beta-agarase; and economically mass-producing the beta-agarase.
Abstract translation: 本发明涉及一种新的β-琼脂糖酶基因及使用其的β-琼脂糖酶的制备方法。 根据本发明的β-琼脂糖酶基因具有在引入不能产生β-琼脂糖酶的双重菌株时直接积聚高浓度的β-琼脂糖酶的优点; 并经济地大规模生产β-琼脂糖酶。
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公开(公告)号：KR100343398B1
公开(公告)日：2002-07-11
申请号：KR1019990001350
申请日：1999-01-19
Applicant: 한국과학기술원
Abstract: 본발명은탈황능력을가진고도나종 CYKS1 균주(KCTC 0431BP)로부터분리된탈황관련유전자인유전자, 전기유전자를포함하는재조합발현벡터, 전기재조합발현벡터로형질전환된재조합대장균및 이를이용한생물학적탈황방법을제공한다. 본발명의재조합대장균을이용하면, 고도나종 CYKS1 균주의탈황관련효소를대량발현할수 있으며, 전기재조합대장균을유기황화합물을포함하는물질이함유된배지에접종하고배양함으로써, 종래의화학적탈황방법에비하여장치비와운전비등을절감하면서화학적탈황방법으로는제거가어려운디벤조티오펜(DBT) 등의유기황화합물로부터용이하게탈황이가능하다.
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公开(公告)号：KR100289501B1
公开(公告)日：2001-05-02
申请号：KR1019990017375
申请日：1999-05-14
Applicant: 한국과학기술원
Abstract: 본발명은미생물을이용하여젖산을생산하는발효공정에있어서, 콩가루, 콩단백질또는콩단백질의가수분해물등 콩으로부터유래한성분을젖산발효미생물배양배지에포함시켜복합질소원으로서이용함으로써, 우수한생산성능및 높은경제성으로젖산을생산할수 있는방법에관한것이다. 본발명은콩 유래성분및 비타민을포함하는배양배지에젖산발효미생물을접종하여배양하고, 전기배양물로부터젖산을수득하는공정을포함한다. 본발명에의하면, 젖산발효미생물의배양을통한젖산의생산에있어서, 효모추출물을질소원으로이용할때와비교하여낮은배지비용으로 60시간안에 120g/L의젖산을생산할수 있다. 이러한결과는종래의방법들과비교해볼 때, 전체공정의경제성을크게차지하는배지성분인효모추출물을곡물인콩을이용하여완전히대체할수 있다는것에중요한의미가있는바, 젖산의대량생산을위한생산비용을획기적으로줄일수 있다.
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