-
公开(公告)号：DE69839709D1
公开(公告)日：2008-08-21
申请号：DE69839709
申请日：1998-12-24
Applicant: CEPHEID
Inventor： POURAHMADI FARZAD , MCMILLIAN WILLIAM A , CHING JESUS , CHANG RONALD , CHRISTEL LEE A , KOVACS GREGORY T , NORTHRUP M ALLEN , PETERSEN KURT E
IPC: B01J19/00 , G01N31/20 , B01D11/00 , B01L3/00 , B01L7/00 , C12M1/00 , C12M1/26 , C12M1/33 , C12M1/34 , C40B60/14 , G01N1/28 , G01N30/60 , G01N33/50 , G01N35/08 , G01N37/00
Abstract: A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g. filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41A and 41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid. A preferred embodiment relates to a device for lysing components of a fluid sample, the device comprising inter alia, an ultrasonic transducer.
-
公开(公告)号：AT400358T
公开(公告)日：2008-07-15
申请号：AT01118242
申请日：1998-12-24
Applicant: CEPHEID
Inventor： POURAHMADI FARZAD , MCMILLIAN WILLIAM A , CHING JESUS , CHANG RONALD , CHRISTEL LEE A , KOVACS GREGORY T , NORTHRUP M ALLEN , PETERSEN KURT E
IPC: B01J19/00 , G01N31/20 , B01D11/00 , B01L3/00 , B01L7/00 , C12M1/00 , C12M1/26 , C12M1/33 , C12M1/34 , C40B60/14 , G01N1/28 , G01N30/60 , G01N33/50 , G01N35/08 , G01N37/00
Abstract: A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g. filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41A and 41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid. A preferred embodiment relates to a device for lysing components of a fluid sample, the device comprising inter alia, an ultrasonic transducer.
-
公开(公告)号：DE60022025T2
公开(公告)日：2006-06-29
申请号：DE60022025
申请日：2000-05-30
Applicant: CEPHEID SUNNYVALE
Inventor： TAYLOR T , BELGRADER PHILLIP , POURAHMADI FRAZAD , MCMILLAN A , CHANG RONALD , SAKAI H , CHING JESUS , PETERSEN E , NORTHRUP ALLEN
-
公开(公告)号：AU758407B2
公开(公告)日：2003-03-20
申请号：AU1947299
申请日：1998-12-24
Applicant: CEPHEID
Inventor： POURAHMADI FARZAD , MCMILLAN WILLIAM A , CHING JESUS , CHANG RONALD , CHRISTEL LEE A , KOVACS GREGORY T A , NORTHRUP M ALLEN , PETERSEN KURT E
IPC: G01N31/20 , B01D11/00 , B01J19/00 , B01L3/00 , B01L7/00 , C12M1/00 , C12M1/26 , C12M1/33 , C12M1/34 , C40B60/14 , G01N1/28 , G01N30/60 , G01N33/50 , G01N35/08 , G01N37/00
Abstract: A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g. filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41A and 41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid. A preferred embodiment relates to a device for lysing components of a fluid sample, the device comprising inter alia, an ultrasonic transducer.
-
公开(公告)号：AU5301000A
公开(公告)日：2000-12-18
申请号：AU5301000
申请日：2000-05-30
Applicant: CEPHEID
-
Patent Agency Ranking
公开(公告)号：CA2373249C
公开(公告)日：2011-08-02
申请号：CA2373249
申请日：2000-05-30
Applicant: CEPHEID
Inventor： TAYLOR MICHAEL T , BELGRADER PHILLIP , POURAHMADI FRAZAD , MCMILLAN WILLIAM A , CHANG RONALD , SAKAI STANLEY H , CHING JESUS , PETERSEN KURT E , NORTHRUP M ALLEN
IPC: C12M1/33 , C12M3/08 , B01L3/00 , B01L7/00 , C12M1/26 , C12N1/00 , G01N1/04 , G01N1/10 , G01N33/48
Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
-
公开(公告)号：DE60014676D1
公开(公告)日：2004-11-11
申请号：DE60014676
申请日：2000-05-30
Applicant: CEPHEID SUNNYVALE
Inventor： PETERSEN E , TAYLOR T , POURAHMADI FARZAD , MCMILLAN A , CHANG RONALD , SAKAI H , CHING JESUS , BELGRADER PHILLIP , NORTHRUP ALLEN , DORITY B
-
公开(公告)号：AU2010257244A1
公开(公告)日：2011-01-13
申请号：AU2010257244
申请日：2010-12-16
Applicant: CEPHEID
Inventor： CHANG RONALD , CHING JESUS , DORITY DOUGLAS BRYAN , ZHANG JIAN PING , WANG JAMES JIAN QUAN , WONG WENDY WINGKEI , PAUL KENDRA LARA , ATTA REUEL VAN
Abstract: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
-
公开(公告)号：AU2005299590B2
公开(公告)日：2010-09-16
申请号：AU2005299590
申请日：2005-10-27
Applicant: CEPHEID
Inventor： CHANG RONALD , DORITY DOUGLAS BRYAN , CHING JESUS , WONG WENDY WINGKEI , ZHANG JIAN PING , ATTA REUEL VAN , PAUL KENDRA LARA , WANG JAMES JIAN QUAN
Abstract: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
-
公开(公告)号：CA2587085A1
公开(公告)日：2006-05-04
申请号：CA2587085
申请日：2005-10-27
Applicant: CEPHEID
Inventor： DORITY DOUGLAS BRYAN , CHING JESUS , ZHANG JIAN PING , WANG JAMES JIAN QUAN , PAUL KENDRA LARA , ATTA REUEL VAN , WONG WENDY WINGKEI , CHANG RONALD
Abstract: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
-
-
-
-
-
-
-
-
-
Search Results
42
records
(took 0.018 seconds)
