公开(公告)号：JPH02249479A

公开(公告)日：1990-10-05

申请号：JP7143689

申请日：1989-03-23

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  MOTOHASHI RYOICHI ,  AIBA SHUICHI ,  GONDA KINJI ,  KAJIO KAZUTO

IPC: G01N35/08 ,  C12M1/34

Abstract: PURPOSE:To enable measurement of an extracellular ATP concentration, an extracellular and intracellular ATP concentration and blank value switching in order in a short time by operating many pumps and injectors corresponding to a measuring object. CONSTITUTION:For measurement of an extracellular ATP (adenosine triphosphate) concentration, the injector 1 is rotated in order to change the passage to the carrier liquid (C1) after supplying a cell suspension (S) to the injector 1 and the injector 2 is simultaneously changed in order to send a luminous agent (E) sandwiching the luminous agent (E) with carrier liquids (C2) after supplying the luminous agent (E) to the injector 2. For measurement of an intracellular ATP concentration, an ATP-extracted solution (R) is injected into the sample passage 3 from the supply passage 10 before a cell suspension (S) injected between the carrier liquid (C1) by the injector 1 reaches a mixer 6. Blank value is measured by supplying only the carrier liquid (C1) and the carrier liquid (C2).

公开(公告)号：JP2000111506A

公开(公告)日：2000-04-21

申请号：JP28576598

申请日：1998-10-07

Applicant: TOA ELECTRONICS

Inventor： HARADA YASUYUKI ,  MURAYAMA JIRO ,  HAKETA YASUSHI ,  SATO TETSUYA ,  KATO EIJI

IPC: G01N27/414 ,  G01N27/06 ,  G01N27/07 ,  G01N27/416

Abstract: PROBLEM TO BE SOLVED: To provide a measuring device with memory for automatically recognizing such information as the type and manufacturing number of an electrode without any need for setting a calibration or correction value each time when the electrode is replaced using a device body and for freely using the electrode for each application. SOLUTION: A measuring device 1 is provided with a device body 20 and a measurement electrode 10. An identification means for identifying the measurement electrode 10 is provided in the measurement electrode 10 or in a cable or a connector for connecting the measurement electrode 10 to the device body 20, and a storage means 21 for recognizing the identification means of the measurement electrode 10 and storing information regarding the measurement electrode 10 is provided in the device body 20.

公开(公告)号：JPH09107994A

公开(公告)日：1997-04-28

申请号：JP29067095

申请日：1995-10-13

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI

IPC: G01N31/22 ,  C12Q1/66 ,  G01N21/76 ,  G01N21/78 ,  G01N33/52

Abstract: PROBLEM TO BE SOLVED: To quickly assay in high sensitivity intracellular ATP for bacteria tests, etc., by subjecting a cell-contg. specimen to extraction with an ATP extractive reagent followed by adding a biochemiluminescent reagent to the resultant liquid extract to conduct a biochemiluminescence in the presence of an enzymatic reaction inhibition-suppressive reagent. SOLUTION: An ATP extractive reagent containing a surfactant (e.g. polyoxyethylene-10-octylphenyl ether) is added to a specimen containing cells (e.g. bacteria) to extract the ATP in the cells, and a luminescent reagent containing luctiferin and luciferase is then added to the resultant liquid extract to conduct a biochemiluminescence by enzymatic reaction, and based on the resultant luminescence, the ATP in the cells is assayed. In this intracellular ATP assay, the biochemiluminescence is conducted in the presence of an enzymatic reaction inhibition-suppressive reagent containing a silicone (e.g. dimethylpolysiloxane) to suppress the inhibition of the enzymatic reaction for the biochemiluminescence by the extractive reagent, thus quickly assaying in high sensitivity the target intracellular ATP for bacteria tests etc.

公开(公告)号：JPH06129989A

公开(公告)日：1994-05-13

申请号：JP30310892

申请日：1992-10-15

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  NISHINO TATSUO ,  TSUNODA HIROSHI

IPC: G01N21/01 ,  G01N21/05 ,  G01N21/76

Abstract: PURPOSE:To provide a chemical light emission detector, wherein the detection of light emission and emitted light quantity with an optical detecting means is done at a constant temperature, an measurement of emitted light quantity due to light emission reaction is done with high precision, stability and reliability with no affect from temperature. CONSTITUTION:A light-shielding case 1 that contains a flaw-cell 2 and a photomultiplier tube 3 is provided in a heat-insulation case 11, and further, a constant- temperature equipment 7 that contains a heater and a cooler is also provided in it. The constant-temperature equipment 7 keeps the atmosphere in the case 1 at a specified temperature, or constant temperature, so the detection of light emission reaction and emitted light quantity of a measured sample, introduced in the flaw-cell 2, with the photomultiplier tube 3, is done at a constant temperature, thus objective is attained.

公开(公告)号：JPH06129988A

公开(公告)日：1994-05-13

申请号：JP30310792

申请日：1992-10-15

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  NISHINO TATSUO ,  TSUNODA HIROSHI

IPC: C12M1/00 ,  G01N21/76 ,  G01N33/50

Abstract: PURPOSE:To provide an in-cell ATP measuring device that allows measuring of ATP amount of both somatic cell and microorganism cell with ease and in a short time without causing error attributable to operators by performing automatic preprocessing and extraction operations with an object sample instead of manual operation by an operator. CONSTITUTION:An entire cell extraction cell 28, a somatic cell extraction cell 29, a means that supplies these cells 28 and 29 with a cell suspended liquid 5, a means that injects entire cell extraction reagent 3 and somatic cell extraction reagent 4 into the supplied cell suspended liquid 5, a means that injects light emission reagent 1 into the obtained entire cell extraction reagent and somatic cell extraction, a chemical light emission detector 30 that detects amount of light emission of the entire cell extraction reagent and somatic cell extraction reagent, and a computer 32 are provided, and the computer 32 controls operation of respective parts. With this, manual operation by an operator is not required, so objective is attained.

公开(公告)号：JPH02249970A

公开(公告)日：1990-10-05

申请号：JP7143589

申请日：1989-03-23

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  MOTOHASHI RYOICHI ,  AIBA SHUICHI ,  GONDA KINJI ,  KAJIWARA KAZUTO

IPC: G01N35/00 ,  C12M1/34 ,  G01N35/08

Abstract: PURPOSE:To prolong the life of a reagent and calibrating liquid by providing a reagent vessel connected to a detector in a thermostatic chamber which maintains the vessel at a room temp. or below. CONSTITUTION:After a sample liquid S is supplied by a pump 7 to an injector 10, the injector 10 is rotated to switch the flow passage to the flow passage of a carrier liquid C1 and the sample liquid S cut off in such a manner is inserted by the carrier liquid C1 fed by a pump 6 and is mixed 12. The flow passage of the sample liquid S is connected to the flow passage from a calibrat ing liquid vessel 1 by a 3-way valve 5 just before the pump 7. The calibrating liquid can be supplied to the ejector 10 in place of the sample liquid S by switching the 3-way valve 5 at the time of calibration. On the other hand, the reagent is supplied from the reagent vessel 2 by a pump 9 to another injector 11 and the reagent cut off by a similarly switching the injector 11 is inserted by the carrier liquid C2 and is mixed 13 by a pump 8. The liquid mixture from the mixers 12, 13 is further mixed 14 and is passed through a detector 15, from which the liquid mixture is discharged. This liquid mixture is processed 16 as an electrical signal and the concn. thereof is determined.