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公开(公告)号：KR100788290B1
公开(公告)日：2007-12-27
申请号：KR1020070014919
申请日：2007-02-13
Applicant: 강원대학교산학협력단
CPC classification number: A01H4/005 , A01H4/001 , A01H4/008 , C12N5/0025 , C12N2500/34 , C12N2501/30
Abstract: A method for mass-propagating Oplopanax elatus Nakai plantlet is provided to establish the in vitro mass-production system of Oplopanax elatus Nakai, one of endangered plant species, thereby mass-producing the Oplopanax elatus Nakai which has useful pharmacological effects and is hard to be propagated. A method for mass-propagating Oplopanax elatus Nakai plantlet comprises the steps of: (a) germinating washed Oplopanax elatus Nakai seeds in an SH medium or a B5 medium at a temperature of 25 deg.C for 16 hours light condition and 8 hours dark condition; (b) culturing a fragment body of the germinated in vitro plant in a liquid medium or a solid medium to induce somatic embryos; (c) culturing the induced somatic embryos in a liquid medium or a solid medium to proliferate them; (d) culturing the proliferated somatic embryos in a liquid medium or a solid medium to redifferentiate a plantlet; and (e) acclimatizing the redifferentiated platelet in soil consisting of bed soil and sand in a mixture ratio of 1:1.
Abstract translation: 提供了一种大规模生产Oplopanax elatus Nakai小植物的方法，建立了濒危植物种类的Oplopanax elatus Nakai的体外批量生产系统，从而大量生产具有有效药理作用的Oplopanax elatus Nakai，难以成为 传播。 大量繁殖Oplopanax elatus Nakai小植物的方法包括以下步骤：（a）在SH培养基或B5培养基中在25℃的温度下将洗涤的Oplopanax elatus Nakai种子发芽16小时光条件和8小时黑暗条件 ; （b）在液体培养基或固体培养基中培养发芽的体外植物的片段体以诱导体细胞胚; （c）在液体培养基或固体培养基中培养诱导的体细胞胚以使其增殖; （d）在液体培养基或固体培养基中培养增殖的体细胞胚以再分化植物; 和（e）以1：1的混合比适应由床土和沙子组成的土壤中的再分化血小板。
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公开(公告)号：KR1020170119872A
公开(公告)日：2017-10-30
申请号：KR1020160048072
申请日：2016-04-20
Applicant: 강원대학교산학협력단
CPC classification number: A01H4/00 , A01H4/001 , A61K8/19 , A61K8/97 , A61Q19/02 , C12N5/0025 , C12N2500/05 , C12N2500/34 , C12N2500/60 , C12N2501/70 , C12N2523/00
Abstract: 본발명은게르마늄을함유하는땃두릅나무부정근의생산방법, 상기방법에의해생산된게르마늄을함유하는땃두릅나무부정근및 상기게르마늄을함유하는땃두릅나무부정근을유효성분으로하는항산화및 피부미백용화장료조성물에관한것이다. 본발명은땃두릅나무부정근배양과정에서, 유기산및 킬레이트를처리하는방법과 2단계배양법을통하여배양하였을때 부정근내 유기게르마늄축적및 생산이효과적으로이루어지며, 상기유기게르마늄을축적한땃두릅나무부정근은피부미백, 항산화활성및 총페놀함량이증가하는효과를나타냄을확인하였다. 본발명의방법에의해제조된부정근을이용하여땃두릅나무의유용성분뿐만아니라유기게르마늄생산에의한고기능성원료를확보하고, 이의피부미백및 항산화증진효과를확인하여산업화에부합되는소재로서의개발을가능하게할 것으로기대된다.
Abstract translation: 本发明涉及抗氧化剂和皮肤增白剂用于容纳ttatdureup木不定根的ttatdureup木不定根化妆品组合物，并通过生产ttatdureup木不定根方法制造含锗的锗，含锗作为活性成分的方法 会的。 通过处理的有机酸，并与两步培养法的螯合有机锗积累和生产有效不定根组成，ttatdureup木不定根累积有机锗皮肤增白的方法在本发明中ttatdureup木材不定根培养过程时，在培养 ，抗氧化活性和总酚含量增加。 使用通过本发明的方法以及固定ttatdureup树的有用成分产生的不定根，移动到有机锗生产的功能性材料，并证实其皮肤美白和抗氧化促进效果可以被开发作为满足工业化的材料 据预计。
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公开(公告)号：KR101514284B1
公开(公告)日：2015-04-22
申请号：KR1020130099733
申请日：2013-08-22
Applicant: 강원대학교산학협력단
Abstract: 본 발명은 땃두릅나무(
Oplopanax elatus ) 추출물 또는 이의 분획물을 유효성분으로 함유하는 암 예방 및 치료용 약학적 조성물에 관한 것으로서, 구체적으로 땃두릅나무 추출물 또는 이의 분획물은 다양한 암세포주에 대하여 유의적인 세포독성 효과, 치사 효과(lethal effect) 및 세포사멸(apoptosis) 효과를 나타내며, 여드름 균에 대한 항균 효과를 나타내므로, 암 예방 및 치료용 약학적 조성물의 유효성분에 효과적으로 사용될 수 있다.-
公开(公告)号：KR1020140083473A
公开(公告)日：2014-07-04
申请号：KR1020120153265
申请日：2012-12-26
Applicant: 강원대학교산학협력단
Abstract: The present invention relates to a method for increasing biomass of ginseng adventitious roots, comprising a step of culturing the ginseng adventitious roots through light emitting diode (LED) irradiation at a red light wavelength range of 625-630 nm. The optimum proliferation conditions for culturing the ginseng adventitious roots through a bioreactor is developed by using the irradiation of the LED in a specific wavelength according to the present invention, thereby establishing the formation and growth of the favorable ginseng adventitious roots and the increase in biomass. Thus, provided can be a method for massively propagating the ginseng adventitious roots through tissue culture, capable of industrially mass-producing the ginseng adventitious roots in a bioreactor, thereby producing high value-added ginseng throughout the year without being affected by climatic and environmental conditions, and supplying the ginseng adventitious roots to various user classes at a low price.
Abstract translation: 本发明涉及一种提高人参不定根生物量的方法，其特征在于，包括通过在625-630nm的红光波长范围的发光二极管（LED）照射培养人参不定根的步骤。 通过使用根据本发明的特定波长的LED的照射来开发通过生物反应器培养人参不定根的最佳增殖条件，从而建立有利的人参不定根的形成和生长以及生物量的增加。 因此，提供的方法是通过组织培养大规模地繁殖人参不定根，能够在生物反应器中工业上大量生产人参不定根，从而在不受气候和环境条件影响的情况下在一年中生产高附加值人参 ，以低价格为各种用户提供人参不定根。
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公开(公告)号：KR1020130027338A
公开(公告)日：2013-03-15
申请号：KR1020110090866
申请日：2011-09-07
Applicant: 강원대학교산학협력단
CPC classification number: C12N15/8218 , C07K14/415 , C12N9/1007 , C12N15/8243 , C12N15/8255 , C12N15/8265 , C12Y201/01068
Abstract: PURPOSE: COMT gene originated from Miscanthus sinensis and its use is provided to use for the yield increase controlling the anti oxidation activation of plants as the phenolic compound contents are controllable in the vector-transformed plant. CONSTITUTION: The method to control the phenolic compound content of plant comprises a step that controls the revelation of COMT gene by transforming recombinant vector in a plant cell which contains COMT [caffeic acid O-methyltransferase] gene originated from Miscanthus sineis; and a step that redifferentiates plants from the transformed plant cells. The phenolic compound content of plant is reduced by inhibiting the revelation of COMT gene. The phenolic compound contents of plant is increased by the overexpression of COMT gene. The phenolic compound of plant is flavonoid or lignine. The inhibition of COMT gene revelation is executed by transforming the plant with the recombinant plant vector in which COMT gene originated from Miscanthus sinensis is inserted in an anti-sense direction. The COMT gene is formed with a base rank of the rank number 1. The plant with reduced content of phenolic compound is produced by inhibiting the expression of COMT gene.
Abstract translation: 目的：COMT基因起源于芒草，其用途用于控制植物的抗氧化活化的产量增加，因为酚类化合物含量在载体转化植物中是可控的。 构成：控制植物酚类化合物含量的方法包括通过在含有源自芒草的COMT [咖啡酸O-甲基转移酶]基因的植物细胞中转化重组载体来控制COMT基因的启动的步骤; 以及将植物从转化的植物细胞重新分化的步骤。 通过抑制COMT基因的启示来减少植物的酚类化合物含量。 通过COMT基因的过表达，植物的酚类化合物含量增加。 植物的酚类化合物是类黄酮或木质素。 通过用来自中国芒草的COMT基因以反义方向插入的重组植物载体转化植物来进行COMT基因启动的抑制。 COMT基因形成为等级1的碱基级别。通过抑制COMT基因的表达产生酚类化合物含量降低的植物。
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Patent Agency Ranking
公开(公告)号：KR101214785B1
公开(公告)日：2012-12-24
申请号：KR1020100037643
申请日：2010-04-23
Applicant: 강원대학교산학협력단
Abstract: 본발명은드라이마리아코다타잎으로부터신초의직접적인고빈도재생방법및 그에따른식물체에관한것으로, 더욱상세하게는드라이마리아코다타() 종자를발아시켜절취한유묘의잎의절편을 NAA; BAP 또는 TDZ; 및수크로스가함유된 MS 배지에서신초를고 빈도(high-frequency)로재생시키는단계를포함하는드라이마리아코다타() 신초의직접적인고빈도재생방법, 상기고빈도로재생된드라이마리아코다타() 신초로부터재분화된드라이마리아코다타식물체의제조방법, 상기방법에따라제조된재분화된드라이마리아코다타식물체및 이의종자를제공한다.
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公开(公告)号：KR1020120128226A
公开(公告)日：2012-11-27
申请号：KR1020110046038
申请日：2011-05-17
Applicant: 강원대학교산학협력단
IPC: A61K36/899 , A61P35/00
CPC classification number: A61K36/899 , A23L3/00 , A23L3/165 , A23L3/3454 , A23L3/3472 , A61K36/00 , A61K36/18 , A61K36/88
Abstract: PURPOSE: A pharmaceutical composition containing Sorghum bicolor extract for preventing or treating cancer is provided to be used in a pharmaceutical composition or functional foods. CONSTITUTION: A pharmaceutical composition for preventing or treating cancer contains Sorghum bicolor extract as an active ingredient. The extract is an hexane fraction, ethyl acetate fraction, butanol fraction, or water fraction which is prepared by fractioning Sorghum bicolor crude extract with hexane, ethyl acetate, butanol, and water.
Abstract translation: 目的：提供含有用于预防或治疗癌症的高粱双色提取物的药物组合物用于药物组合物或功能性食品中。 构成：用于预防或治疗癌症的药物组合物含有高粱双色提取物作为活性成分。 提取物是通过用己烷，乙酸乙酯，丁醇和水分离高粱双色粗提物制备的己烷级分，乙酸乙酯级分，丁醇级分或水级分。
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公开(公告)号：KR1020110092497A
公开(公告)日：2011-08-18
申请号：KR1020100011945
申请日：2010-02-09
Applicant: 강원대학교산학협력단
IPC: A23L19/00 , A23L3/26 , A23L1/30 , A61K36/8962
CPC classification number: A23L27/105 , A23L5/30 , A23L33/105 , A61K36/8962
Abstract: PURPOSE: Onion with the improved content of quercetin, and a producing method thereof are provided to radiate far infrared rays to dried onion for improving the content of the quercetin. CONSTITUTION: A producing method of onion with the improved content of quercetin comprises a step of radiating far infrared rays at 80deg C to dried onion for 20~30minutes. The onion is used to obtain an onion extract with the improved content of the quercetin by extracting the onion with a solvent. The onion extract is for producing functional food.
Abstract translation: 目的：提供槲皮素含量提高的洋葱及其制备方法，以将远红外线辐射到干洋葱，以改善槲皮素的含量。 构成：槲皮素含量提高的洋葱的生产方法包括将80℃的远红外线辐射干洋葱20-30分钟的步骤。 洋葱用于通过用溶剂萃取洋葱来获得具有改善的槲皮素含量的洋葱提取物。 洋葱提取物用于生产功能性食品。
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公开(公告)号：KR1020070111409A
公开(公告)日：2007-11-21
申请号：KR1020070095740
申请日：2007-09-20
Applicant: 강원대학교산학협력단
CPC classification number: A23L33/105 , A23V2200/302 , A23V2250/21
Abstract: A kaempferol compound isolated from a kenaf extract(Hibiscus cannabinus) is provided to be used as active components of functional food with antibacterial and antioxidant activity. A functional food contains 0.1 to 20% by weight of a kaempferol compound represented by the formula(1) as an effective component from a kenaf extract and an excipient. The kaempferol compound is prepared by the steps of: obtaining crude extract by extracting kenaf leaf with methanol; obtaining a water layer and organic layer by extracting the crude extract with a solvent selected from water, aliphatic alcohol, aqueous alcohol solutions, hexane, chloroform, ethyl acetate, benzene and butanol; obtaining active fractions by absorbing the water layer and organic layer into a first silica chromatography column and eluting a fraction while changing the ratio of toluene and acetone or acetone and methanol; obtaining active fractions by absorbing the active fractions into a second silica chromatography column and eluting a fraction while changing the ratio of water and methanol(10:0 to 5:5); subjecting the fractions to gel filter chromatography(Sephadex LH-20) with 40% methanol; and finally recrystallizing the purified fractions and purifying a single active compound by high speed liquid chromatography.
Abstract translation: 提供从洋麻提取物（木槿大麻）中分离出的山奈酚化合物作为具有抗菌和抗氧化活性的功能性食品的活性成分。 功能性食品含有0.1〜20重量％的由式（1）表示的山奈酚化合物作为来自洋麻提取物和赋形剂的有效成分。 山奈酚化合物通过以下步骤制备：通过用甲醇提取洋麻叶来获得粗提物; 通过使用选自水，脂族醇，醇溶液，己烷，氯仿，乙酸乙酯，苯和丁醇的溶剂提取粗提取物得到水层和有机层; 通过将水层和有机层吸收到第一硅胶色谱柱中并在改变甲苯和丙酮或丙酮和甲醇的比例的同时洗脱级分获得活性级分; 通过将活性级分吸收到第二硅胶色谱柱中并在改变水和甲醇的比例（10:0至5:5）的同时洗脱级分来获得活性级分; 用馏分进行凝胶过滤层析（Sephadex LH-20）与40％甲醇; 最后将纯化的级分重结晶，并通过高速液相色谱纯化单一活性化合物。
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公开(公告)号：KR1020060058301A
公开(公告)日：2006-05-30
申请号：KR1020040097276
申请日：2004-11-25
Applicant: 강원대학교산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q1/6827 , C12Q1/6895 , C12Q2563/113
Abstract: 본 발명은 스틸벤 화합물을 생성하는 형질전환 지황(
Rhemannia glutinosa L.) 식물체로부터 스틸벤 합성유전자를 분석하는 분석방법에 관한 것으로서, 보다 상세하게는 레스베라트롤 및 관련 파생물을 합성을 기호화하고 있는 스텔벤 합성 유전자의 지황 형질전환 식물체로부터 스틸벤 합성유전자의 발현을 확인하고, 상기 형질전환 식물체에서 다른 유전자의 발현양상의 변화를 확인할 수 있는 DNA 칩을 이용한 유전자 분석 방법에 관한 것이다. 본 발명의 DNA 칩을 이용한 유전자 분석방법은 생물체에 구성되어 있는 어떠한 유전자의 핵심부분(active site)을 프로브(probe)로 사용하여 해당 유전자의 발현 여부를 신속하게 검정할 수 있으며, 유전자가 도입된 형질전환 식물체를 이용하여 다수의 유전자에 대한 발현양상을 비교할 수 있으므로, 변형된 유전자가 삽입된 식물체에서 해당 유전자 및 다른 유전자의 발현양상 분석에 유용하게 이용될 수 있다.
DNA 칩, 프로브 DNA, 유전자 분석.
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