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    오리 간염바이러스 1형의 감별진단방법
    22.
    发明授权
    오리 간염바이러스 1형의 감별진단방법 有权
    差异诊断鸭肝炎病毒1型的方法

    公开(公告)号：KR100858169B1

    公开(公告)日：2008-09-17

    申请号：KR1020060056569

    申请日：2006-06-22

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 김민철 ,  권용국 ,  조성준 ,  이윤정 ,  조영미 ,  권혁만 ,  권준헌 ,  김재홍

    IPC: C12Q1/68

    Abstract: 본 발명은 오리 간염바이러스 1형(duck hepatitis virus type 1) 유전자의 감별진단방법(differential diagnostic method)에 관한 것으로, 더욱 상세하게는 2C 유전자, 캡시드(capsid) 유전자 및 5'-UTR(untranslated region) 유전자를 표적(target)하는 특이 프라이머를 사용하여 RT-PCR 방법으로 오리 간염바이러스 1형과 유전자형이 다른 오리 간염바이러스 유전자를 감별 진단하는 방법에 관한 것이다. 또한 본 발명에 따른 감별진단방법은 조류 유래의 다른 병원성 RNA 바이러스들과도 신속하고 정확하게 감별적으로 진단하는데 유용하다.
    오리 간염바이러스 1형 및 아형, PCR, 5′-UTR 유전자, 캡시트 유전자, 3D 유전자

    신속 면역크로마토그라피법에 의한 Ｈ5형 고병원성 및 일반조류인플루엔자 바이러스 항원 진단 스트립 및 그 제조방법
    23.
    发明公开
    신속 면역크로마토그라피법에 의한 Ｈ5형 고병원성 및 일반조류인플루엔자 바이러스 항원 진단 스트립 및 그 제조방법 有权
    用于H5型高致病性流感病毒性流行性感冒病毒和使用快速免疫印迹的一般流行性流感病毒抗原的诊断条带及其制备方法

    公开(公告)号：KR1020070072945A

    公开(公告)日：2007-07-10

    申请号：KR1020060000325

    申请日：2006-01-03

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 바이오노트

    Inventor： 이윤정 ,  최준구 ,  이은경 ,  권용국 ,  권준헌 ,  김재홍 ,  성환우 ,  오진식 ,  조영식 ,  조병기

    IPC: G01N33/53

    Abstract: A diagnostic strip for a H5 type highly pathogenic avian influenza and general avian influenza is provided to conveniently and rapidly diagnose whether a bird is infected by the H5 type highly pathogenic avian influenza and general avian influenza or not from stool in situ without special testing equipment. The diagnostic strip is characterized in that a monoclonal antibody common to general avian influenza virus in which hemaglutinin antigens are H1 to H15 and a monoclonal antibody specific to a highly pathogenic avian influenza in which a hemaglutinin antigen is H5 are used for diagnosing the highly pathogenic and the general avian influenza in which the hemaglutinin antigen is H5 from stool or tissue of chicken. The method for preparing the diagnostic strip comprises the steps of: (a) respectively absorbing the monoclonal antibody specific to the highly pathogenic avian influenza virus in which the hemaglutinin antigen is H5 and the monoclonal antibody common to general avian influenza virus into a membrane; (b) respectively attaching the antibody absorbed membrane obtained from the step(a) to gold particles so as to prepare an antibody-gold conjugate and then mixing it; and (c) precipitating the conjugate obtained from the step(b) in a basic material and then drying it.

    Abstract translation: 提供一种H5型高致病性禽流感和一般禽流感的诊断条，以方便快捷地诊断鸟类是否由H5型高致病性禽流感和一般禽流感感染，或者不是在没有特殊检测设备的情况下从粪便中感染。 诊断条的特征在于，其中血凝素抗原为H1至H15的一般性禽流感病毒共有的单克隆抗体和其中血凝素抗原为H5的高致病性禽流感特异性的单克隆抗体用于诊断高致病性和 其中血凝素抗原为粪便或鸡组织中H5的一般性禽流感。 制备诊断条的方法包括以下步骤：（a）分别将其中血凝素抗原为H5的高致病性禽流感病毒和一般禽流感病毒共有的单克隆抗体特异性的单克隆抗体吸收到膜中; （b）将由步骤（a）获得的抗体吸收膜分别附着于金颗粒上，制备抗体 - 金缀合物，然后混合; 和（c）将由步骤（b）获得的缀合物沉淀在碱性材料中，然后干燥。

    동물용 불활화 백신의 제조방법
    24.
    发明公开
    동물용 불활화 백신의 제조방법 有权
    用于疫苗制备的低致病性AVIAN INFLUENZA H9N2病毒株，疫苗及其制备方法

    公开(公告)号：KR1020070005867A

    公开(公告)日：2007-01-10

    申请号：KR1020050060920

    申请日：2005-07-06

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 성환우 ,  이윤정 ,  최준구 ,  이은경 ,  권준헌 ,  김재홍

    IPC: C12N7/01 ,  C12N7/04

    Abstract: A virus strain for preparing a vaccine is provided to have excellent defending effect on low pathogenic avian influenza H9N2 virus strain. And a vaccine is provided to be able to effectively prevent diseases caused by the low pathogenic avian influenza H9N2 recording the high mortality of bird. The low pathogenic avian influenza H9N2 virus strain for preparing vaccine is characterized in that a motif of hemangioblast protein cleavage site amino acid sequence has TSGR and it shows the defending capacity against domestic low pathogenic avian influenza virus. The method for preparing a vaccine for the low pathogenic avian influenza H9N2 virus strain comprises the steps of: (a) inactivating the low pathogenic avian influenza H9N2 virus strain; and (b) mixing the inactivated virus with an adequate excipient.

    Abstract translation: 提供用于制备疫苗的病毒株，以对低致病性禽流感H9N2病毒株具有优异的防御作用。 并提供疫苗能够有效预防由低致病性禽流感H9N2引起的疾病，记录鸟类的高死亡率。 用于制备疫苗的低致病性禽流感H9N2病毒株的特征在于成血管细胞蛋白切割位点氨基酸序列的基序具有TSGR，并且显示出针对国内低致病性禽流感病毒的防御能力。 用于制备低致病性禽流感H9N2病毒株的疫苗的方法包括以下步骤：（a）灭活低致病性禽流感H9N2病毒株; 和（b）将灭活的病毒与足够的赋形剂混合。

    조류인플루엔자 바이러스의 유전자재조합 단백질 및 이를함유하는 조류인플루엔자 진단액
    25.
    发明公开
    조류인플루엔자 바이러스의 유전자재조합 단백질 및 이를함유하는 조류인플루엔자 진단액 无效
    AVIAN流感病毒的重组蛋白和含有其的AVIAN流感病毒的诊断组合物

    公开(公告)号：KR1020060132154A

    公开(公告)日：2006-12-21

    申请号：KR1020050052324

    申请日：2005-06-17

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 성환우 ,  이윤정 ,  최준구 ,  김재홍

    IPC: C12N15/09 ,  C12N15/63 ,  C12N15/64

    Abstract: A recombinant protein of avian influenza virus and a diagnostic composition of avian influenza containing the same protein are provided to mass produce the antigen for diagnosis of avian influenza cheaply, so that the disease caused by avian influenza is prevented. The method for producing the recombinant nucleus protein of avian influenza virus comprises the steps of: (1) amplifying the gene encoding nucleocapsid having the nucleotide sequence of SEQ ID NO:1 as a common protein of MS96 strain with PCR, and cloning the PCR amplified gene into the TA cloning vector; (2) re-isolating the NP gene inserted into the TA cloning vector with a restriction enzyme EcoR I, and inserting the isolated NP gene into the baculovirus transition vector pBacPAK8 to prepare the baculovirus transition vector pBacAINP; (3) co-transfecting the Sf21 cell with the baculovirus and the transition vector pBacAINP to a recombinant baculovirus(rAINP) expressing the avian influenza virus NP protein; and (4) verifying the expression of avian influenza virus NP(nucleus protein) in the Sf21 cell by using a monoclonal antibody specific to the avian influenza virus NP.

    Abstract translation: 提供禽流感病毒的重组蛋白和含有相同蛋白质的禽流感诊断组合物，以便大量生产用于禽流感诊断的抗原，从而防止禽流感引起的疾病。 制备禽流感病毒重组核蛋白的方法包括以下步骤：（1）用PCR扩增编码具有SEQ ID NO：1的核苷酸序列的核衣壳的基因作为MS96菌株的常见蛋白质，并将PCR扩增 基因进入TA克隆载体; （2）用限制酶EcoR I重新分离插入TA克隆载体的NP基因，将分离的NP基因插入杆状病毒转移载体pBacPAK8中以制备杆状病毒转移载体pBacAINP; （3）将杆状病毒和过渡载体pBacAINP的Sf21细胞共转染至表达禽流感病毒NP蛋白的重组杆状病毒（rAINP）; 和（4）通过使用特异于禽流感病毒NP的单克隆抗体来验证Sf21细胞中禽流感病毒NP（核蛋白）的表达。

    기상레이더 3차원 반사도 자료를 이용한 밝은 띠 영역의 탐색 방법 및 그 시스템
    29.
    发明授权
    기상레이더 3차원 반사도 자료를 이용한 밝은 띠 영역의 탐색 방법 및 그 시스템 有权
    使用三维雷达反射率检测亮度条的方法和系统

    公开(公告)号：KR101255966B1

    公开(公告)日：2013-04-23

    申请号：KR1020120028965

    申请日：2012-03-21

    Applicant: 경북대학교 산학협력단 ,  대한민국(기상청장)

    Inventor： 이규원 ,  이수향 ,  정성화 ,  박종서 ,  유철환 ,  이윤정 ,  한혜영

    IPC: G01S13/95

    CPC classification number: Y02A90/18

    Abstract: PURPOSE: A method for searching a bright band region using meteorological radar 3-D reflectivity data using a mean reflectivity vertical profile and a system thereof are provided to be utilized as a fundamental resource to classify a type of rainfall particle with altitude and a type of rainfall and as a fundamental resource for accurate ground precipitation estimation. CONSTITUTION: A bright band region search system(100) using meteorological radar three-dimensional reflectivity data includes a meteorological radar three-dimensional reflectivity data collection unit(110), a mean reflectivity vertical profile generation unit(120) and a bright band region search unit(130). The meteorological radar three-dimensional reflectivity data collection unit collects meteorological radar three-dimensional reflectivity data observed in a meteorological radar system. The mean reflectivity vertical profile generation unit generates a vertical profile displaying mean reflectivity spaced by a predetermined distance with altitude using the collected meteorological radar three-dimensional reflectivity data. The bright band region search unit generates a respective vertical profile of each parameter including the first derivative, the second derivative, the curvature and the first derivative of the curvature of the generated mean reflectivity vertical profile, or the logarithm of the generated mean reflectivity vertical profile and a respective vertical profile of each parameter including the first derivative, the second derivative, the curvature and the first derivative of the curvature. The bright band region search unit searches for a peak, an upper part and a lower part of the bright band region using the mean reflectivity vertical profile and the vertical profile of each parameter. [Reference numerals] (10) Meteorological radar system; (110) Meteorological radar three-dimensional reflectivity data collection unit; (120) Mean reflectivity vertical profile generation unit; (130) Bright band region search unit;

    Abstract translation: 目的：提供一种使用平均反射率垂直剖面及其系统的气象雷达3-D反射率数据搜索亮带区域的方法，作为基本资源，用于对降雨颗粒类型与高度进行分类， 作为精确降雨估算的基础资源。 构成：使用气象雷达三维反射率数据的明带搜索系统（100）包括气象雷达三维反射率数据采集单元（110），平均反射率垂直剖面生成单元（120）和亮带区域搜索 单元（130）。 气象雷达三维反射率数据采集单元收集气象雷达系统中观测到的气象雷达三维反射率数据。 平均反射率垂直剖面生成单元使用收集的气象雷达三维反射率数据生成垂直剖面显示与高度间隔预定距离的平均反射率。 亮带区域搜索单元生成包括所产生的平均反射率垂直剖面的曲率的一阶导数，二阶导数，曲率和一阶导数的每个参数的相应垂直分布，或者所生成的平均反射率垂直分布的对数 以及包括曲率的一阶导数，二阶导数，曲率和一阶导数的每个参数的相应垂直轮廓。 亮带区域搜索单元使用平均反射率垂直分布和每个参数的垂直分布来搜索亮带区域的峰值，上部和下部。 （附图标记）（10）气象雷达系统; （110）气象雷达三维反射率数据采集单元; （120）平均反射率垂直剖面生成单元; （130）明带搜索单元;

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