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公开(公告)号：KR1020090006536A
公开(公告)日：2009-01-15
申请号：KR1020070069963
申请日：2007-07-12
Applicant: 대한민국(농촌진흥청장)
Abstract: A method for culturing the nuclear transfer embryos is provided to increase the generation rate of blastocyst and to improve the quality of blastocyst, thereby enhancing the production efficiency of cloned animal. A method for culturing the nuclear transfer embryos comprises the steps of locating the nuclear transfer embryos in the culture mediumcontaining the sperm cytosolic factor and applying voltage to it; and moving the nuclear transfer embryos to the culture medium for culture and culturing it. Preferably the concentration of the sperm cytosolic factor is 100-300 mug/mL.
Abstract translation: 提供培养核转移胚胎的方法，以增加囊胚发生率，提高胚泡质量，提高克隆动物的生产效率。 用于培养核转移胚胎的方法包括将核转移胚胎定位在含有精子细胞溶质因子的培养基中并向其施加电压的步骤; 并将核转移胚移动到培养基中进行培养和培养。 优选精子胞质因子的浓度为100-300ug / mL。
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公开(公告)号：KR1020170114797A
公开(公告)日：2017-10-16
申请号：KR1020160042349
申请日：2016-04-06
Applicant: 대한민국(농촌진흥청장)
IPC: C12N9/10 , C12N15/63 , C12N15/877 , A01K67/027
CPC classification number: C12N9/1051 , A01K67/0276 , A01K2227/107 , C12N15/63 , C12N15/8778 , C12Y204/01087
Abstract: 본발명은돼지의이소글로보트리헥소실세라마이드신타제(iGb3s) 넉아웃세포주및 그의제조방법에관한것으로서, 보다상세하게는 gal 에피토프(epitope)의발현을제어하여초급성면역거부반응이현저하게감소된돼지를제조할수 있는돼지의 iGb3s 및 GT가동시에넉아웃된세포주및 그의제조방법에관한것이다.
Abstract translation: 本发明是异丙基文章猪机器人树己基神经酰胺损失合酶（iGb3s）敲除细胞系，并涉及制造方法，更具体地涉及控制加仑表位（表位）的表达的方法显著超急性排斥反应 对于能够生产减少的猪的猪的iGb3s，并且在GT操作时敲除细胞系，以及其生产方法。
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公开(公告)号：KR101435635B1
公开(公告)日：2014-08-29
申请号：KR1020120108343
申请日：2012-09-27
Applicant: 전남대학교산학협력단 , 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/54 , A01K67/027
Abstract: 알파 1,3-갈락토실트랜스퍼라아제(α1,3-galactosyl transferase, α1,3-GT) 유전자의 위치에 보체 조절 단백질(decay accelerating factor, DAF) 유전자와 인간의 알파 1,2-푸코실트랜스퍼라아제(human α1,2-fucosyltransferase, HT) 유전자를 적중시킬 수 있는 넉인 벡터, 상기 벡터가 도입된 세포, 상기 세포로 핵이식된 수정란, 상기 벡터에 의해 넉 아웃된 형질전환 동물, 및 이의 제조방법에 관한 것이다. 본 발명의 넉인 벡터는 이종장기이식에서 발생하는 초급성 거부반응이 억제된 형질전환 동물의 제조에 이용될 수 있으므로, 보다 효율적인 이종장기이식에 사용될 수 있는 형질전환 동물의 생산에 널리 활용될 수 있을 것이다.
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公开(公告)号：KR1020110029255A
公开(公告)日：2011-03-23
申请号：KR1020090086846
申请日：2009-09-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6844
Abstract: PURPOSE: A marker and method for identifying individual cloned cow are provided to distinguish cloned cow and donor cow through a genetic method. CONSTITUTION: A marker for identifying cloned cow comprises a primer of sequence numbers 1-7, which specifically bind to ND5, D-loop, and 12S rDNA. A method for identifying cloned cow comprises: a step of isolating mitochondria DNA from a subject cow; and a step of amplifying ND5, D-loop, and 12S rDNA gene region of mitochondria DNA using a pair of sequence number 1 and 2 or 3.
Abstract translation: 目的：提供用于鉴定单克隆牛的标记和方法，以通过遗传方法区分克隆的牛和供体牛。 构成：用于鉴定克隆牛的标记物包含序列号为1-7的引物，其特异性结合ND5，D环和12S rDNA。 鉴定克隆的牛的方法包括：从受试者母牛中分离线粒体DNA的步骤; 并使用一对序列号1和2或3扩增线粒体DNA的ND5，D-loop和12S rDNA基因区域的步骤。
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公开(公告)号：KR100325306B1
公开(公告)日：2002-02-25
申请号：KR1019990034178
申请日：1999-08-18
Applicant: 대한민국(농촌진흥청장)
IPC: A61D19/00
Abstract: 본발명은번식우발정검출기에관한것으로, 좀더상세하게는발정난소를검출하여이를무선으로전달하여외부에서소의발정상태를확인할수 있도록하는장치에관한것이다. 이에본 발명의번식우발정검출기는소의발정상태를검출하여소마다다른코드로외부에번식우발정검출송신기와, 이신호를수신하여표시장치를통해운용자에게보여주는발정검출수신기와, 발정검출수신기가검출한발정난소의정보를외부에서입력받을수 있는호출기나이동전화를포함하여이루어지는것을특징으로한다.
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Patent Agency Ranking
公开(公告)号：KR1020010081456A
公开(公告)日：2001-08-29
申请号：KR1020000006888
申请日：2000-02-14
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/00
CPC classification number: A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/108 , A01K2267/01 , C12N15/8509
Abstract: PURPOSE: A method for producing transgenic porcines for producing human erythropoietin(EPO) is provided, thereby human erythropoietin(EPO) can be mass-produced. CONSTITUTION: The method for producing transgenic porcines comprising the steps of: cloning the genomic DNA of human erythropoietin(EPO) using a WAP promoter isolated from the mammary gland of mouse by polymerase chain reaction(PCR); preparing the EPO expression vector containing human erythropoietin(hEPO) and SV40 poly A gene; injecting eCG hormone and hCG hormone into the fertilized eggs of porcine to induce overovulation; recovering the fertilized eggs and inserting the above DNA into a male cell nucleus using a manipulator; transplanting the cell into a surrogate porcine mother; and producing transgenic porcines having DNA represented by SEQ ID NO: 1 from the surrogate porcine mother.
Abstract translation: 目的：提供生产人促红细胞生成素（EPO）的转基因猪的方法，从而大量生产人促红细胞生成素（EPO）。 构成：生产转基因猪的方法，包括以下步骤：使用通过聚合酶链反应（PCR）从小鼠乳腺分离的WAP启动子克隆人促红细胞生成素（EPO）的基因组DNA; 制备含有促红细胞生成素（hEPO）和SV40聚A基因的EPO表达载体; 将eCG激素和hCG激素注射到猪的受精卵中以诱导过度排卵; 回收受精卵，并使用机械手将上述DNA插入雄性细胞核; 将细胞移植到替代猪母亲中; 并从代孕猪母体生产具有由SEQ ID NO：1表示的DNA的转基因猪。
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公开(公告)号：KR1020010018288A
公开(公告)日：2001-03-05
申请号：KR1019990034179
申请日：1999-08-18
Applicant: 대한민국(농촌진흥청장)
IPC: A01K11/00
CPC classification number: A01K11/006 , A01K11/004
Abstract: PURPOSE: Transmitter-receiver is provided which uses the signal of a transmitter to communicate with a receiver with different code as livestock so that it is helpful in finding livestock wanted from a barn with various kinds of livestock. CONSTITUTION: The transmitter-receiver to identify kinds of livestock comprises the parts of: a transmitter wherein an input device(100) to input name or specific number of livestock, an encoder(110) to encode the output signal from the input device, a display device, a wireless transmitter(120) to print the encoded signal in wireless and a power device to supply power; and a receiver wherein a wireless receiver to receive the wireless signal, a decoder to decode the received signal, a comparison device to compare specific code stored with the decoded code, a notification device to notify if the compared codes are same and a power device.
Abstract translation: 目的：提供发射机 - 接收机，其使用发射机的信号与具有不同编码的接收机与家畜通信，从而有助于从具有各种牲畜的谷仓中寻找想要的牲畜。 构成：用于识别牲畜的种类的发送器 - 接收器包括以下部分：发送器，其中输入名称或特定数量的牲畜的输入装置（100），对来自输入装置的输出信号进行编码的编码器（110） 显示装置，以无线方式打印编码信号的无线发射机（120）和用于供电的功率装置; 以及接收器，其中接收无线信号的无线接收器，解码接收信号的解码器，比较装置，用于比较与解码的代码一起存储的特定代码;通知装置，用于通知比较的代码是否相同;以及功率器件。
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公开(公告)号：KR101896945B1
公开(公告)日：2018-09-13
申请号：KR1020160150365
申请日：2016-11-11
Applicant: 대한민국(농촌진흥청장)
IPC: C12N5/075
Abstract: 본발명은개과동물의난관상피세포및 MEGS(Mammary Epithelial Growth supplement)를포함하는개과동물의미성숙란체외성숙용조성물및 이를이용한개과동물의미성숙란체외성숙률향상방법에관한것이다. 상기한본 발명에의하면, 개과동물의미성숙란의체외성숙률을높일수 있는이점이있다.
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公开(公告)号：KR101891556B1
公开(公告)日：2018-08-27
申请号：KR1020160150355
申请日：2016-11-11
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은 a) 암컷동물에서채혈한혈액으로부터생식주기조절호르몬의농도를분석하여피크(peak)를확인하는단계; 및 b) 상기호르몬농도의피크로부터 66시간내지 78시간후를배란시기로예측하는단계;를포함하는암컷동물의배란시기예측방법및 이를이용한암컷동물의성숙난자채란방법에관한것이다. 본발명에의하면, 성숙란채란시기의예측정확도를높이고농도측정기기에따른변이를최소화하여분석방법에따른수치를보정할필요가없는이점이있다.
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