넉인 벡터 및 이를 이용한 장기이식용 형질전환 동물의 제조방법
    2.
    发明公开
    넉인 벡터 및 이를 이용한 장기이식용 형질전환 동물의 제조방법 有权
    用于制作移植用途的转基因动物的插入矢量和方法

    公开(公告)号:KR1020140052093A

    公开(公告)日:2014-05-07

    申请号:KR1020120108343

    申请日:2012-09-27

    Abstract: The present invention relates to a knock-in vector that can target a complement regulatory protein (decay accelerating factor, DAF) gene and a human alpha 1,2-fucosyltransferase (HT) gene in a position of an alpha 1,3-galactosyl transferase (alpha 1,3-GT) gene, a cell transduced with the vector, a fertilized egg that is nuclear transplanted in the cell, a transgenic animal that is knocked out by the vector, and a method for producing the same. The knock-in vector of the present invention can be used to produce a transgenic animal in which hyperacute rejection that occurs in xeno-transplantation is controlled, so that the knock-in vector can be widely used for the production of a transgenic animal that can be used for effective xeno-transplantation.

    Abstract translation: 本发明涉及可以靶向α1,3-半乳糖基转移酶位置的补体调节蛋白(衰变加速因子,DAF)基因和人α1,2-岩藻糖基转移酶(HT)基因的敲入载体 (α1T-GT)基因,用载体转导的细胞,被移植到细胞中的受精卵,被载体敲除的转基因动物及其制备方法。 本发明的敲入载体可用于产生其中控制异种移植中发生超急性排斥反应的转基因动物,使得敲入载体可广泛用于生产转基因动物,其可以 用于有效的异种移植。

    한우의 수정적기 측정용 조성물, 키트 및 이를 이용한 한우 수정적기의 측정방법
    5.
    发明公开
    한우의 수정적기 측정용 조성물, 키트 및 이를 이용한 한우 수정적기의 측정방법 有权
    用于确定汉诺威最佳行车时间的组合物,套件和方法

    公开(公告)号:KR1020130125553A

    公开(公告)日:2013-11-19

    申请号:KR1020120049173

    申请日:2012-05-09

    Abstract: The present invention relates to a composition for measuring the optimal time of insemination in Hanwoo (Korean beef) and a method for measuring the optimal time of insemination using the same and, more specifically, to a composition for measuring the optimal time of insemination using proteins which are specifically expressed at the mating season of Hanwoo and a measuring method using the same. According to the present invention, provided is a kit and a method for scientifically diagnosing the optimal time of insemination with reliability in a process of recognizing development and insemination which are important for producing calves through artificial insemination.

    Abstract translation: 本发明涉及一种用于测定Hanwoo(韩国牛肉)最佳授精时间的组合物,以及使用该组合物测定最佳授精授精时间的方法,更具体地说,涉及使用蛋白质测定最佳授精时间的组合物 这是在Hanwoo的交配季节具体表达的,并且使用它们的测量方法。 根据本发明,提供了一种用于通过人工授精对生产犊牛重要的识别发育和授精的过程中可靠地科学地诊断授精最佳时间的试剂盒和方法。

    CYP4F16 유전자가 넉다운된 형질전환마우스 및 그 제조방법
    10.
    发明公开
    CYP4F16 유전자가 넉다운된 형질전환마우스 및 그 제조방법 有权
    CYP4F16 KNOCK-DOWN鼠标及其制造方法

    公开(公告)号:KR1020130080572A

    公开(公告)日:2013-07-15

    申请号:KR1020120001427

    申请日:2012-01-05

    Abstract: PURPOSE: A transgenic mouse in which CYP4F16 gene is knocked-down and a manufacturing method thereof are provided to inquire a cause of occurrence of a sleeping disease among immune-related genes generated after infestation of trypanosome and to develop new medicine for curing the diseases. CONSTITUTION: A siRNA expression recombinant vector for mouse CYP4F16 knocked-down is identified by a promoter and SEQ ID No. 1 that is operably connected to the promoter. The promoter is a U6 promoter. The recombinant vector is introduced to a transgenic mouse. A manufacturing method of a transgenic mouse in which CYP4F16 gene expression is knocked-down comprises the following steps of: (a) manufacturing a siRNA expression cassette for expressing CYP4F16 that is represented by a promoter and SEQ ID No. 1 that is operably connected to the promoter; (b) introducing the recombinant fused gene to a fertilized egg of a mouse; and (c) producing breeders by implanting the fertilized egg into a surrogate mother.

    Abstract translation: 目的:提供CYP4F16基因被敲除的转基因小鼠及其制造方法,用于询问在锥虫感染后产生的免疫相关基因中发生睡眠疾病的原因,并开发用于治疗疾病的新药。 构成:用于小鼠CYP4F16敲除的siRNA表达重组载体通过与启动子可操作连接的启动子和SEQ ID No.1来鉴定。 启动子是U6启动子。 将重组载体导入转基因小鼠。 其中CYP4F16基因表达被敲除的转基因小鼠的制造方法包括以下步骤:(a)制备用于表达CYP4F16的siRNA表达盒,所述siRNA表达盒由启动子和SEQ ID No.1表示,所述启动子和SEQ ID No.1可操作地连接到 启动子; (b)将重组融合基因导入小鼠受精卵; 和(c)通过将受精卵植入替代母亲来培育育种者。

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