公开(公告)号：KR1020170040541A

公开(公告)日：2017-04-13

申请号：KR1020150139655

申请日：2015-10-05

Applicant: 대한민국(농촌진흥청장)

Inventor： 이창묵 ,  윤상홍 ,  구본성 ,  한범수 ,  심준수 ,  이영석

IPC: C12N9/42 ,  C12N15/70

Abstract: 본발명은동애등에유충장내에존재하는난배양미생물로부터분리한유기용매에강한β-글루코시다아제유전자및 이의용도에관한것이다. 본발명은동애등에() 유충장내의난배양미생물로부터비환원성β-글리코시드말단의β-1,4-글리코시딕결합을분해하는신규효소유전자를분리하고, 이를대량으로발현하는신종균주를개발하여식물성고분자다당류를이용하는다양한산업적공정과정에서효율적으로적용할수 있다. 또한, 본발명에따른신규효소유전자의분해효과는산업공정과정외에식품발효산업과제지/직물산업, 알코올생산공정, 의약품제조및 식물다당류의당화작용등에이용될수 있다.

公开(公告)号：KR1020160011946A

公开(公告)日：2016-02-02

申请号：KR1020140093289

申请日：2014-07-23

Applicant: 대한민국(농촌진흥청장)

Inventor： 한범수 ,  심준수 ,  정하영

IPC: C12N15/82 ,  A01H5/00

CPC classification number: C12N15/8218 ,  C12N15/113 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8285 ,  C12N2310/14

Abstract: 본발명은선충유전자인마이오신라이트체인(myosin light chain) 유전자및 이의용도에관한것으로, 구체적으로마이오신라이트체인유전자에대한 RNAi 염기서열을포함하는뿌리혹선충저항성재조합벡터를이용하여뿌리혹선충저항성을가지는형질전환식물체를제작하였으므로다양한식물체에서선충에의한피해를줄일수 있는기반기술로유용하게이용될것이다.

Abstract translation: 本发明涉及作为来自线虫的基因的肌球蛋白轻链基因及其用途。 更具体地说，所产生的是具有根结线虫抗性重组载体的具有根结线虫抗性的转基因植物，其包含肌球蛋白轻链基因的核糖核酸干扰（RNAi）干扰（RNAi）碱基序列。 因此，该植物可用作减少各种植物线虫损伤的基础技术。 为此，对根结线虫抗性的重组载体含有由序列号1表示的碱基序列组成的肌球蛋白轻链基因的RNAi碱基序列。

公开(公告)号：KR1020100058827A

公开(公告)日：2010-06-04

申请号：KR1020080117379

申请日：2008-11-25

Applicant: 대한민국(농촌진흥청장)

Inventor： 김영미 ,  임명호 ,  김정선 ,  한범수 ,  한유경 ,  하선화 ,  강상호

IPC: C12N15/10 ,  C12N15/09 ,  C12N15/05

Abstract: PURPOSE: A pollen-specific expression promoter is provided to reduce time and labor for inducing male sterility and to produce superior seeds. CONSTITUTION: A pollen-specific expression promoter PBrRF1 comprises a sequence of sequence number 1. A primer for amplifying the pollen-specific expression promoter PBrRF1 comprises oligonucleotides of sequence number 2 and sequence number 3. An expression vector pBGWFS7-PBrRF1 contains a promoter of sequence number 1. A method for preparing the expression vector pBGWFS7-PBrRF1 comprises: a step of isolating a promoter site of sequence number 1 from a Brassica campestris ssp. Pekinensis; a step of preparing a primer set of sequence numbers 2 and 3 from a genomic DNA isolated from the Arabidopsis thaliana; a step of isolating the promoter site through DNA amplification; a step of performing recombination of Pdonr221 vector and amplified PCR product to obtain a cloning vector of pDONR-PBrRF1 and pDONR-P35S; a step of performing LR recombination of pBGWFS7 vector containing a reporter system; and a step of transforming a plant body with the expression vector pBGWFS7-PBrRF1.

Abstract translation: 目的：提供花粉特异性表达启动子，以减少诱导雄性不育和产生优质种子的时间和劳动。 构成：花粉特异性表达启动子PBrRF1包含序列号1的序列。扩增花粉特异性表达启动子PBrRF1的引物包含序列号2和序列号3的寡核苷酸。表达载体pBGWFS7-PBrRF1含有序列的启动子 1。一种制备表达载体pBGWFS7-PBrRF1的方法，包括：分离序列号1的启动子位点与芸苔属芥菜的步骤。 大白菜; 从拟南芥分离的基因组DNA制备序列号2和3的引物组的步骤; 通过DNA扩增分离启动子位点的步骤; 进行Pdonr221载体和扩增的PCR产物重组的步骤，得到pDONR-PBrRF1和pDONR-P35S的克隆载体; 执行含有报告系统的pBGWFS7载体的LR重组的步骤; 以及用表达载体pBGWFS7-PBrRF1转化植物体的步骤。

公开(公告)号：KR1020090006684A

公开(公告)日：2009-01-15

申请号：KR1020070070277

申请日：2007-07-12

Applicant: 대한민국(농촌진흥청장) ,  제주특별자치도(농업기술원) ,  코아바이오시스템 주식회사

Inventor： 한범수 ,  김용환 ,  김종범 ,  오상수 ,  김성용 ,  박용원 ,  이진성 ,  서현희

IPC: C12N15/66 ,  C12N15/09 ,  C12N15/05

Abstract: A recombinant vector, a cell transformed by the vector, a transformed plant cell, and a method for preparing the transformed hairy root are provided to produce the tissue-type plasminogen activator protein massively with a low cost. A recombinant vector comprises the polynucleotide which codes a promoter, a TEV (tobacco etch viru) leader sequence connected with the primer, the glucose-regulated endoplasmic reticular protein derived from alfalfa, and the tissue-type plasminogen activator protein. Preferably the TEV leader sequence has the base sequence represented by the sequence number 1.

Abstract translation: 提供重组载体，由载体转化的细胞，转化的植物细胞和用于制备转化的毛根的方法，以低成本大量生产组织型纤溶酶原激活物蛋白。 重组载体包含编码启动子的多核苷酸，与引物连接的TEV（烟草蚀刻viru）前导序列，来自苜蓿的葡萄糖调节的内质网状蛋白质和组织型纤溶酶原激活蛋白。 优选地，TEV前导序列具有由序列号1表示的碱基序列。

公开(公告)号：KR100685520B1

公开(公告)日：2007-03-09

申请号：KR1020060000783

申请日：2006-01-04

Applicant: 대한민국(농촌진흥청장)

Inventor： 하선화 ,  황선갑 ,  김경환 ,  김종범 ,  한범수 ,  박종석 ,  김용환 ,  김영미 ,  서석철

IPC: C12N15/63 ,  C12N15/29

Abstract: A seed specific promoter having a novel nucleotide sequence, a primer for amplifying the promoter, an expression vector including the promoter and a plant cell transformed by the expression vector are provided to be able to limit the expression on seeds without influencing on the plant growth by seed-specifically inducing a target protein gene. The seed specific promoter consists of SEQ ID : NO. 1. The primer for amplifying seed specific promoter consists of an oligonucleotide consisting of SEQ ID : NO. 2 and an oligonucleotide consisting of SEQ ID : NO. 3. The expression vector pBGWFS7-PfOle20 deposited as a deposition number of KACC 95044P includes a promoter sequence described as SEQ ID : NO. 1. The plant cell is transformed by the expression vector pBGWFS7-PfOle20.

Abstract translation: 提供具有新核苷酸序列的种子特异性启动子，用于扩增启动子的引物，包含启动子的表达载体和由表达载体转化的植物细胞，以能够限制在种子上的表达而不影响植物生长， 种子特异性诱导靶蛋白基因。 种子特异性启动子由SEQ ID NO： 用于扩增种子特异性启动子的引物由SEQ ID NO：1组成的寡核苷酸组成。 2和由SEQ ID NO：2组成的寡核苷酸。 作为KACC 95044P的保藏号保藏的表达载体pBGWFS7-PfOle20包含如SEQ ID NO：1所述的启动子序列。 1.植物细胞被表达载体pBGWFS7-PfOle20转化。

公开(公告)号：KR100606612B1

公开(公告)日：2006-07-31

申请号：KR1020040117577

申请日：2004-12-31

Applicant: 대한민국(농촌진흥청장)

Inventor： 김종범 ,  박종석 ,  김용환 ,  한범수 ,  김정봉 ,  김경환 ,  하선화

IPC: C12N15/52 ,  C12N15/29

Abstract: 본 발명은 녹차 어린잎 유래 델타-6 불포화효소 유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이 형질전환체의 제조방법에 관한 것으로서, 더욱 상세하게는, 서열번호 1로 기재되는 염기서열로 이루어지는, 녹차 어린잎 유래 델타-6 불포화효소를 코딩하는 유전자, 이 유전자를 포함하는 발현벡터 pYES2/CT-D6DES, 이 발현벡터로 형질전환된 효모(
Saccharomyces cerevisiae ) 형질전환체(수탁번호: KACC 93021) 및 이 형질전환체의 제조방법에 관한 것이다.
델타-6 불포화효소, pYES2/CT, Saccharomyces cerevisiae