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    1,4―ＢＤＯ 생산을 위한 유전자 스크리닝 방법
    23.
    发明公开
    1,4―ＢＤＯ 생산을 위한 유전자 스크리닝 방법 审中-实审
    鉴定1,4-BDO生产基因的筛选方法

    公开(公告)号：KR1020150023175A

    公开(公告)日：2015-03-05

    申请号：KR1020130100567

    申请日：2013-08-23

    Applicant: 삼성전자주식회사

    Inventor： 조화영 ,  박진환 ,  정유경 ,  박재찬

    IPC: G01N33/68 ,  C12Q1/02 ,  C12N1/21

    CPC classification number: C12P7/18 ,  C07K14/34 ,  C12N9/0006 ,  C12N9/1025 ,  C12Q1/48 ,  C12Y101/01027 ,  C12Y203/03001 ,  G01N2333/91045

    Abstract: The present invention relates to a method for screening genes used for increasing 1,4-butanediol (BDO) production by using proteomics data. In the present invention, 1,4-BDO productivity is increased when a Ncgl0630 (citrate synthase) protein and a Ncgl2145 (hypothetical protein) protein are overly expressed wherein the Ncgl0630 (citrate synthase) protein and the Ncgl2145 (hypothetical protein) protein are screened via the method which is used to find proteins related to 1,4-BDO productivity and accordingly increases productivity, thereby being widely applicable industrially.

    Abstract translation: 本发明涉及通过使用蛋白质组学数据筛选用于增加1,4-丁二醇（BDO）生产的基因的方法。 在本发明中，当Ncgl0630（柠檬酸合成酶）蛋白和Ncgl2145（假想蛋白））蛋白被过度表达时，其中筛选出Ncgl0630（柠檬酸合成酶）蛋白和Ncgl2145（假想蛋白））蛋白质时，1,4-BDO生产力增加 通过用于发现与1,4-BDO生产率相关的蛋白质的方法，从而提高生产率，从而在工业上广泛应用。

    1,4-ＢＤＯ 생성능이 개선된 변이 미생물 및 이를 이용한 1,4-ＢＤＯ 의 제조방법
    26.
    发明公开
    1,4-ＢＤＯ 생성능이 개선된 변이 미생물 및 이를 이용한 1,4-ＢＤＯ 의 제조방법 审中-实审
    具有改善1,4-BDO生产能力的突变微生物及其制备1,4-BDO的方法

    公开(公告)号：KR1020140015086A

    公开(公告)日：2014-02-06

    申请号：KR1020120082815

    申请日：2012-07-27

    Applicant: 삼성전자주식회사

    Inventor： 이규상 ,  이영민 ,  이우용 ,  구현민 ,  김진우 ,  박영경 ,  박진환 ,  조화영 ,  박재찬

    IPC: C12N1/21 ,  C12N15/52 ,  C12N15/74 ,  C12P7/16

    CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N15/52 ,  C12N15/77 ,  C12Y101/01037 ,  C12Y101/05004 ,  Y02P20/52

    Abstract: One embodiment of the present invention relates to a metabolism network model of Corynebacterium glutamicum for producing 1,4-BDO by Corynebacterium glutamicum, which is a strain that does not produce 1,4-BDO in a natural state. On the basis of the network model, metabolic properties of Corynebacterium glutamicum are analyzed. By predicting effects of a deletion target enzyme (ldhA, mqo or mdh) in a state in which Cat1, sucD, 4hbD, cat2 and adhE are included, and removing them, production efficiency of 1,4-BDO and increase of 1,4-BDO under the same fermentation conditions are confirmed. In addition, transformation microorganism produced by the method can produce 1,4-BDO in high efficiency, and therefore, can be effectively used in the industries.

    Abstract translation: 本发明的一个实施方案涉及谷氨酸棒杆菌的代谢网络模型，用于通过谷氨酸棒杆菌生产1,4-BDO，其是在天然状态下不产生1,4-BDO的菌株。 在网络模型的基础上，分析谷氨酸棒杆菌的代谢特性。 通过预测在包含Cat1，sucD，4hbD，cat2和adhE的状态下缺失目标酶（ldhA，mqo或mdh）的作用并除去它们，1,4-BDO的生产效率和增加1,4 -BDO在相同的发酵条件下得到确认。 另外，通过该方法生产的转化微生物可以高效率地生产1,4-BDO，因此可以有效地用于工业中。

    3-하이드록시프로피온산의 생산을 위한 유전자 조작
    27.
    发明公开
    3-하이드록시프로피온산의 생산을 위한 유전자 조작 审中-实审
    用于生产3-羟基丙酸的遗传修饰

    公开(公告)号：KR1020130001509A

    公开(公告)日：2013-01-04

    申请号：KR1020110062312

    申请日：2011-06-27

    Applicant: 삼성전자주식회사

    Inventor： 김재영 ,  박성민 ,  박영경 ,  유병조 ,  박재찬 ,  조화영 ,  구현민

    IPC: C12N15/53 ,  C12N15/63 ,  C12P7/52

    CPC classification number: C12P7/42 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y203/01008

    Abstract: PURPOSE: A recombinant microorganism which produces 3-hydroxypropionic acid(3-HP) using a malonic semialdehyde reduction pathway is provided to remarkably enhance 3-HP productivity by gene manipulation. CONSTITUTION: A recombinant microorganism for producing 3-hydroxypropionic acid has a pathway for sequentially producing pyruvate, acetyl Co, malonyl-CoA, and malonic semialdehyde. The microorganism includes Escherichia sp., Saccharomyces sp., or Kluyveromyces sp. A gene encoding lactate dehydrogenase is IdhA or a homolog or a variant thereof. A gene encoding phosphotransacetylase is pta or a homolog or a variant thereof.

    Abstract translation: 目的：提供使用丙二醛还原途径产生3-羟基丙酸（3-HP）的重组微生物，通过基因操作显着提高3-HP生产力。 构成：用于产生3-羟基丙酸的重组微生物具有顺序产生丙酮酸，乙酰辅酶A，丙二酰辅酶A和丙二醛半醛的途径。 微生物包括大肠杆菌，酵母属或克鲁维酵母属。 编码乳酸脱氢酶的基因是IdhA或其同源物或其变体。 编码磷酸转乙酰酶的基因是pta或其同系物或其变体。

    젖산의 고효율 생산을 위한 변형 미생물
    28.
    发明公开
    젖산의 고효율 생산을 위한 변형 미생물 审中-实审
    用于高效生产乳酸的改性微生物

    公开(公告)号：KR1020130001121A

    公开(公告)日：2013-01-03

    申请号：KR1020120061819

    申请日：2012-06-08

    Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

    Inventor： 정순천 ,  구현민 ,  김재영 ,  김지은 ,  김진우 ,  박영경 ,  이소영 ,  조화영 ,  권대혁 ,  박재찬

    IPC: C12N1/19 ,  C12N1/21 ,  C12N15/53 ,  C12P7/56

    Abstract: PURPOSE: A modified microorganism for producing lactic acids and a method for producing lactic acids are provided to produce lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids with high efficiency has LDH activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, and Rattus norvegicus. The modified microorganism is yeast or bacteria. The modified microorganism is E.coli or Kluyveromyces marxianus. The modified microorganism produces lactic acids with 12.2% or more of glucose. An expression vector for producing the modified microorganism comprises: a replication origin; a promoter; a polynucleotide; and a terminator. A method for producing lactic acids comprises: a step of culturing the modified microorganism in a medium containing glucose; and a step of collecting lactic acids from the culture.

    Abstract translation: 目的：提供用于生产乳酸的改性微生物和生产乳酸的方法，以在酸性条件下高效生产乳酸。 构成：用于高效生产乳酸的改良微生物具有日本芍药，鸟腥草，Tur ps属，and and属的LDH活性。 修饰的微生物是酵母或细菌。 修饰的微生物是大肠杆菌或马克斯克鲁维酵母（Kluyveromyces marxianus）。 经修饰的微生物产生具有12.2％或更多葡萄糖的乳酸。 用于产生修饰微生物的表达载体包括：复制起点; 启动子 多核苷酸; 和终结者。 制备乳酸的方法包括：在含有葡萄糖的培养基中培养经修饰的微生物的步骤; 以及从培养物中收集乳酸的步骤。

    젖산의 고효율 생산을 위한 변형 미생물
    29.
    发明公开
    젖산의 고효율 생산을 위한 변형 미생물 无效
    用于高效生产乳酸的改性微生物

    公开(公告)号：KR1020130001103A

    公开(公告)日：2013-01-03

    申请号：KR1020110139520

    申请日：2011-12-21

    Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

    Inventor： 정순천 ,  구현민 ,  김재영 ,  김지은 ,  김진우 ,  박영경 ,  이소영 ,  조화영 ,  권대혁 ,  박재찬

    IPC: C12N15/63 ,  C12N1/21 ,  C12N15/53 ,  C12P7/56

    Abstract: PURPOSE: A modified microorganism for producing lactic acids is provided to prepare lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids has a lactate dehydrogenase(LDH) activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus. The modified microorganism is Escherichia sp. or Kluyveromyces sp. The modified microorganism produces lactic acids with 34% or more of glucose. An expression vector contains: a replication origin for constructing the modified microroganisms; a promoter; a polynucleotide coding LDH activation of one or more species selected from the group consisting of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus; and a terminator. The replication origin is ARS/CEN replication origin.

    Abstract translation: 目的：提供一种用于生产乳酸的改性微生物，用于在酸性条件下高效制备乳酸。 构成：用于生产乳酸的改良微生物具有日本芍药（Ornisorhynchus anatinus，Tursiops truncates）或褐藻（Rattus norvegicus）的乳酸脱氢酶（LDH）活性。 修饰的微生物是大肠杆菌属 或克鲁维酵母属 经修饰的微生物产生具有34％以上葡萄糖的乳酸。 表达载体包含：用于构建修饰的微结构的复制起点; 启动子 编码一种或多种选自以下的物质的LDH活化的多核苷酸：日本Pe us，鸟，us us us，，，;;;;;;;;;;;;;;;;;; 和终结者。 复制起点是ARS / CEN复制来源。

    산-생산 균주의 고속 선별 방법
    30.
    发明公开
    산-생산 균주의 고속 선별 방법 无效
    生产微生物的高通量筛选

    公开(公告)号：KR1020130000640A

    公开(公告)日：2013-01-03

    申请号：KR1020110061290

    申请日：2011-06-23

    Applicant: 삼성전자주식회사

    Inventor： 조화영 ,  유병조 ,  박재찬 ,  박성민 ,  구현민 ,  이주영

    IPC: C12Q1/04 ,  C12N1/20 ,  C12N1/14

    CPC classification number: G01N21/80 ,  C12Q1/045 ,  Y02A50/451

    Abstract: PURPOSE: A high throughput screening system and method using a pH indicator are provided to accurately, quickly, and easily screen strains which highly produce acids. CONSTITUTION: A system for screening acid-producing stains contains the acid-producing strains, a medium, two or more pH indicators. The acid includes citric acid, itaconic acid, succinic acid, fumaric acid, glycolic acid, pyruvic acid, acetic acid, glutamic acid, malic acid, maleic acid, 3-hydroxypropionic acid(3-HP), butyric acid, or gluconic acid. The strains are Bacillus sp., Streptococcus sp., Streptomyces sp., Staphylococcus sp., Enterococcus sp., Lactobacillus sp., Lactococcus sp., Clostridium sp., Geobacillus sp., Escherichia. Coli sp., Pseudomonas sp., Salmonella sp., Campylobacter sp., Helicobacter sp., Flavobactenum sp., Fusobacterium sp., llyobacter sp., Neisseria sp., Candida sp., Hansenula sp., Kluyveromyces sp., Pichia sp., Saccharomyces sp., Schizosaccharomyces sp., or Yarrowta sp.

    Abstract translation: 目的：提供高通量筛选系统和使用pH指示剂的方法来准确，快速，容易地筛选高度产生酸的菌株。 构成：用于筛选产酸污渍的系统包含酸生产菌株，中等，两种或更多种pH指示剂。 酸包括柠檬酸，衣康酸，琥珀酸，富马酸，乙醇酸，丙酮酸，乙酸，谷氨酸，苹果酸，马来酸，3-羟基丙酸（3-HP），丁酸或葡萄糖酸。 菌株是芽孢杆菌属，链球菌属，链霉菌属，葡萄球菌属，肠球菌属，乳杆菌属，乳球菌属，梭菌属，地芽孢杆菌属，埃希氏菌属。 大肠杆菌，假单胞菌属，沙门氏菌属，弯曲菌属，幽门螺杆菌属，黄杆菌属，福氏杆菌属，丝杆菌属，奈瑟氏球菌属，假丝酵母​​属，汉逊酵母属，克鲁维酵母菌属，毕赤酵母属 ，酵母属（Saccharomyces sp。），粟酒裂殖酵母属（Schizosaccharomyces sp。）或Yarrowta sp。

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