UNIDADES DE EXPRESION PSOD Y USO DE LAS MISMAS EN LA REGULACION DE LA TRANSCRIPCION Y LA EXPRESION DE GENES

    公开(公告)号:AR047152A1

    公开(公告)日:2006-01-11

    申请号:ARP040104737

    申请日:2004-12-17

    Applicant: BASF AG

    Abstract: Se refiere al uso de secuencias de ácidos nucleicos para la regulacion de la transcripcion y la expresion de genes, a los nuevos promotores y unidades de expresion propiamente dichos, a procedimientos para la modificacion o activacion del grado de transcripcion y/o grado de expresion de genes, a casetes de expresion que contienen las unidades de expresion, a microorganismos modificados genéticamente con grado de transcripcion y/o grado de expresion modificado o activado, así como también a procedimientos para la preparacion de productos biosintéticos por medio del cultivo de los microorganismos genéticamente modificados. Reivindicacion 1: Uso de un ácido nucleico con actividad promotora, que contiene: A) la secuencia de ácidos nucleicos SEQ. ID. NO. 1 o B) una secuencia derivada de esta secuencia por sustitucion, insercion o delecion de nucleotidos, la que presenta una identidad de por lo menos 90 % a nivel de ácidos nucleicos con la secuencia SEQ. ID. NO. 1, o C) una secuencia de ácidos nucleicos, que hibrida con la secuencia de ácidos nucleicos SEQ. ID. NO. 1 bajo condiciones severas o D) fragmentos funcionalmente equivalentes de las secuencias de A), B) o C), para la transcripcion de genes.

    METODOS PARA L A PREPARACION DE UN QUIMICO FINO POR FERMENTACION

    公开(公告)号:AR047058A1

    公开(公告)日:2006-01-04

    申请号:ARP040104742

    申请日:2004-12-17

    Applicant: BASF AG

    Abstract: Comprende métodos para aumentar la produccion de un compuesto químico fino, por ejemplo, lisina a partir de un microorganismo, por ejemplo, Corynebacterium mediante la desregulacion de un gen codificador de una enzima, es decir, glicerol quinasa. Se proveen métodos para incrementar la produccion de lisina en Corynebacterium glutamicum a través del incremento de la expresion de la actividad de glicerol quinasa. También se provee un proceso para la produccion de lisina a través de la regulacion del flujo de carbono oxalacetato (OAA).Se proveen métodos para la produccion de lisina a través de la utilizacion de fructosa o sacarosa como fuente de carbono. Reivindicacion 1: Un método para aumentar el flujo metabolico a través de la vía de pentosafosfatos en un microorganismo que comprenda el cultivo de dicho microorganismo que contenga un gen que se desregula bajo condiciones tales que se aumenta el flujo metabolico a través de la vía de pentosafosfatos. Reivindicacion 49: Un microorganismo recombinante que comprende un gen de biosíntesis de pentosafosfato desregulado.

    25.
    发明专利
    未知

    公开(公告)号:DE10359595A1

    公开(公告)日:2005-07-28

    申请号:DE10359595

    申请日:2003-12-18

    Applicant: BASF AG

    Abstract: Use of a nucleic acid (I) with promoter activity for transcribing genes, where (I) is (a) a 164 bp sequence (SEQ ID No,:1); (b) a variant of (SEQ ID No.:1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; (c) a sequence that hybridizes to (SEQ ID No.:1) under stringent conditions; or (d) a functionally equivalent fragment of (a)-(c), is new. Independent claims are also included for : (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence ggaggga (53) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequence tagagt (52) as a -10 region for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (52) or (53).

    26.
    发明专利
    未知

    公开(公告)号:DE10359594A1

    公开(公告)日:2005-07-28

    申请号:DE10359594

    申请日:2003-12-18

    Applicant: BASF AG

    Abstract: Use, for transcribing genes, of a nucleic acid (I) with promoter activity, where (I) is a 186 base pair sequence (1), reproduced; a variant of (1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; a sequence that hybridizes to (1) under stringent conditions; or a functionally equivalent fragment of them, is new. Independent claims are also included for the following: (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence aggagga (42) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequences tagttt (39), taggat (40) or tgcgct (41) as -10 regions for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (39)-(42).

Patent Agency Ranking