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公开(公告)号：GB2502409A
公开(公告)日：2013-11-27
申请号：GB201303939
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A droplet based assay comprising an emulsion including droplets packed in a three-dimensional arrangement, the droplets are reacted, such as thermocycled in this packaged form and then passed serially through a detector region to analyse individual droplets. Other inventions relate to formation of a plurality of emsulsions together and collecting data from individual droplets, these assays may be specific methods of nucleic acid amplification which include thermocycling and that may be conducted from a multiwall plate array of samples, a droplet transporter may pick-up droplets from a sample holder .
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公开(公告)号：DE3642551A1
公开(公告)日：1987-07-09
申请号：DE3642551
申请日：1986-12-12
Applicant: BIO RAD LABORATORIES
Inventor： SLEETER DONALD D , FERNWOOD GEORGE G , BURD SAMUEL
IPC: C08F2/10 , G01N27/26 , G01N27/447 , B01D13/04 , B01D13/00 , B01D57/02 , C08F20/56 , C08J5/18 , C08L33/26 , G01N27/28
Abstract: A gel used for electrophoresis is cast in a gel enclosure in which the gel will ultimately be used during the electrophoresis, by a novel method and using novel apparatus which provide for quick assembly and avoidance of leaks. The method and apparatus involve a wettable, preferably porous, membrane such as filter paper, which is thoroughly wetted with gel forming solution, then placed over the bottom opening of the gel enclosure and held in place until the gel on the membrane sets. Sealing of any cracks, gaps or irregularities along the rim of the opening is thereby achieved by the capillary action of the gel forming solution. The enclosure itself is then filled with gel forming solution. Once the latter has set, the membrane is removed.
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公开(公告)号：GB2164735A
公开(公告)日：1986-03-26
申请号：GB8514185
申请日：1985-06-05
Applicant: BIO RAD LABORATORIES
Inventor： FERNWOOD GEORGE GRAIL , BURD SAMUEL
IPC: G01N27/26 , F26B5/04 , F26B9/00 , G01N27/447 , F26B9/04
Abstract: An apparatus for drying gel slabs supplies heat to a horizontal gel slab from a heating plate above the slab, while drawing a vacuum beneath. The heating plate is weighted and has a flat lower surface at least coextensive with the gel slab. The slab itself is placed on a porous support pad in a vacuum chamber enclosed at the top with a flexible cover which clings to the gel and seals to the perimeter of the chamber when a vacuum is applied from below. The heating plate rests on top of the cover under gravitational force, providing even heating across the entire expanse of the gel slab and for the entire duration of the drying process, resulting in a continuous and uniform vapor flow downward from the gel slab, a minimization of internal stresses, and reduced susceptibility of the gel to cracking.
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公开(公告)号：AU2009297108B2
公开(公告)日：2015-02-12
申请号：AU2009297108
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON JR BILLY WAYNE , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MAKAREWICZ JR ANTHONY JOSEPH , BELGRADER PHILLIP
IPC: G01N21/00
Abstract: Systems, including apparatus and methods, for performing assays. These systems may involve separating sample components by partitioning them into droplets or other partitions, amplifying or otherwise reacting the components within the droplets, detecting the amplified components, or characteristics thereof, and/or analyzing the resulting data, among others.
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公开(公告)号：GB2504241B8
公开(公告)日：2014-10-15
申请号：GB201319376
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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Patent Agency Ranking
公开(公告)号：GB2504241A8
公开(公告)日：2014-10-15
申请号：GB201319376
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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公开(公告)号：GB2502409A8
公开(公告)日：2014-10-15
申请号：GB201303939
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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公开(公告)号：CA3075139A1
公开(公告)日：2010-04-01
申请号：CA3075139
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MAKAREWICZ ANTHONY JOSEPH , BELGRADER PHILLIP
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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公开(公告)号：GB2257160A
公开(公告)日：1993-01-06
申请号：GB9214064
申请日：1992-07-01
Applicant: BIO RAD LABORATORIES
Inventor： BURD SAMUEL
IPC: G01N27/447
Abstract: The block comprises three recesses serving as a reservoir 60 for buffer solution. an injection port 61 for introduction of fluids by a syringe, and outlet 62 (see Fig 3b not shown) which communicates with the capillary tube, the recesses being connected by ducts which meet at a common junction 66 inside the block, and the duct communicating the reservoir 60 with the junction being opened or closed by a valve eg. by a valve with a stem having a conical tip which mates with conical portion 67 of the duct serving as a valve seat. The buffer reservoir 60 communicates with overflow passage 69 separated from the reservoir by wall 70 to permit escape of excess liquid (Fig. 3d not shown). The block permits filling of the capillary, injection of the sample into the capillary, changing of the solution in the buffer reservoir, and flushing out of the entire block without disconnecting the block from the capillary cartridge.
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公开(公告)号：GB2226572A
公开(公告)日：1990-07-04
申请号：GB8926264
申请日：1989-11-21
Applicant: BIO RAD LABORATORIES
Inventor： BURD SAMUEL
IPC: G01N27/447
Abstract: A capillary tube for use in high performance capillary electrophoresis is enclosed in a cartridge of generally planar configuration, formed by two flat plates joined together along raised edges to define a shallow enclosed chamber to retain the looped capillary tube, the plates having aligned windows between which a segment of the capillary tube passes, thereby forming a light path for zone detection. The termini of the capillary are embedded in fittings extending from the exterior of the cartridge, which mate with complementary fittings on inlet and outlet blocks which contain buffer reservoirs with controlled hydrostatic heads. The inlet block is specially equipped with ducts and fittings to permit filling of the capillary, injection of the sample into the capillary, changing of the solution in the buffer reservoir, and flushing out of the entire block without disconnecting the block from the capillary cartridge. By securing the cartridge containing the capillary tube into its holder, the capillary tube is correctly aligned in the detector light path.
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