公开(公告)号：DE60022025D1

公开(公告)日：2005-09-22

申请号：DE60022025

申请日：2000-05-30

Applicant: CEPHEID SUNNYVALE

Inventor： TAYLOR T ,  BELGRADER PHILLIP ,  POURAHMADI FRAZAD ,  MCMILLAN A ,  CHANG RONALD ,  SAKAI H ,  CHING JESUS ,  PETERSEN E ,  NORTHRUP ALLEN

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

公开(公告)号：AT302263T

公开(公告)日：2005-09-15

申请号：AT00937895

申请日：2000-05-30

Applicant: CEPHEID

Inventor： TAYLOR MICHAEL T ,  BELGRADER PHILLIP ,  POURAHMADI FRAZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  PETERSEN KURT E ,  NORTHRUP M ALLEN

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

公开(公告)号：AU764060B2

公开(公告)日：2003-08-07

申请号：AU8364801

申请日：2001-10-26

Applicant: CEPHEID

Inventor： POURAHMADI FARZAD ,  MCMILLAN WILLIAM A ,  CHING JESUS ,  CHANG RONALD ,  CHRISTEL LEE A ,  KOVACS GREGORY T A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E

IPC: C12M1/42 ,  G01N1/28 ,  G01N1/40

公开(公告)号：CA2374423A1

公开(公告)日：2000-12-07

申请号：CA2374423

申请日：2000-05-30

Applicant: CEPHEID

Inventor： CHANG RONALD ,  SAKAI STANLEY H ,  MCMILLAN WILLIAM A ,  POURAHMADI FARZAD ,  TAYLOR MICHAEL T ,  PETERSEN KURT E ,  BELGRADER PHILLIP ,  DORITY DOUGLAS B ,  NORTHRUP M ALLEN ,  CHING JESUS

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

Abstract: A cartridge for separating a desired analyte from a fluid sample has a sampl e flow path and a lysing chamber (86) in the sample flow path. The lysing chamber contains at least one filter for capturing cells or viruses from the sample as the sample flows through the lysing chamber. Beads are also dispos ed in the lysing chamber for rupturing the cells or viruses to release the analyte therefrom. An analyte flow path extends from the lysing chamber and diverges from the sample flow path. The analyte flow path preferably leads t o a reaction chamber for chemically reacting and optically detecting the analyte. The cartridge also includes at least one flow controller (e.g., valves) for directing the sample into the waste chamber after the sample flo ws through the lysing chamber and for directing the analyte separated from the sample into the analyte flow path.

公开(公告)号：CA2373249A1

公开(公告)日：2000-12-07

申请号：CA2373249

申请日：2000-05-30

Applicant: CEPHEID

Inventor： POURAHMADI FRAZAD ,  MCMILLAN WILLIAM A ,  TAYLOR MICHAEL T ,  BELGRADER PHILLIP ,  CHING JESUS ,  NORTHRUP M ALLEN ,  PETERSEN KURT E ,  SAKAI STANLEY H ,  CHANG RONALD

IPC: C12M1/33 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48 ,  C12M3/08

Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least on e flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

公开(公告)号：CA2312102A1

公开(公告)日：1999-07-08

申请号：CA2312102

申请日：1998-12-24

Applicant: CEPHEID

Inventor： CHRISTEL LEE ALLAN ,  NORTHRUP M ALLEN ,  POURAHMADI FARZAD ,  PETERSEN KURT E ,  KOVACS GREGORY T A ,  CHING JESUS ,  MCMILLAN WIILIAM A ,  CHANG RONALD

IPC: G01N31/20 ,  B01D11/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/26 ,  C12M1/33 ,  C12M1/34 ,  C40B60/14 ,  G01N1/28 ,  G01N30/60 ,  G01N33/50 ,  G01N35/08 ,  G01N37/00

Abstract: A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g., filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41A and 41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid.

公开(公告)号：HK1184195A1

公开(公告)日：2014-01-17

申请号：HK13111753

申请日：2013-10-18

Applicant: CEPHEID

Inventor： CHANG RONALD ,  CHING JESUS ,  DORITY DOUGLAS BRYAN ,  ZHANG JIAN PING ,  WANG JAMES JIAN QUAN ,  WONG WENDY WINGKEI ,  PAUL KENDRA LARA ,  ATTA REUEL VAN

IPC: C12Q20060101 ,  C12M20060101 ,  C12N20060101

公开(公告)号：CA2374423C

公开(公告)日：2013-04-09

申请号：CA2374423

申请日：2000-05-30

Applicant: CEPHEID

Inventor： PETERSEN KURT E ,  TAYLOR MICHAEL T ,  POURAHMADI FARZAD ,  MCMILLAN WILLIAM A ,  CHANG RONALD ,  SAKAI STANLEY H ,  CHING JESUS ,  BELGRADER PHILLIP ,  NORTHRUP M ALLEN ,  DORITY DOUGLAS B

IPC: C12M1/33 ,  C12M3/08 ,  B01L3/00 ,  B01L7/00 ,  C12M1/26 ,  C12N1/00 ,  G01N1/04 ,  G01N1/10 ,  G01N33/48

Abstract: A cartridge for separating a desired analyte from a fluid sample has a sample flow path and a lysing chamber (86) in the sample flow path. The lysing chamber contains at least one filter for capturing cells or viruses from the sample as the sample flows through the lysing chamber. Beads are also disposed in the lysing chamber for rupturing the cells or viruses to release the analyte therefrom. An analyte flow path extends from the lysing chamber and diverges from the sample flow path. The analyte flow path preferably leads to a reaction chamber for chemically reacting and optically detecting the analyte. The cartridge also includes at least one flow controller (e.g., valves) for directing the sample into the waste chamber after the sample flows through the lysing chamber and for directing the analyte separated from the sample into the analyte flow path.

公开(公告)号：HK1115163A1

公开(公告)日：2008-11-21

申请号：HK08105089

申请日：2008-05-07

Applicant: CEPHEID

Inventor： CHANG RONALD ,  CHING JESUS ,  DORITY DOUGLAS BRYAN ,  ZHANG JIAN PING ,  WANG JAMES JIAN QUAN ,  WONG WENDY WINGKEI ,  PAUL KENDRA LARA ,  ATTA REUEL VAN

IPC: C12P20060101 ,  A21C20060101

Abstract: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

公开(公告)号：DK1179585T3

公开(公告)日：2008-11-10

申请号：DK01118242

申请日：1998-12-24

Applicant: CEPHEID

Inventor： POURAHMADI FARZAD ,  CHING JESUS ,  CHANG RONALD ,  PETERSEN KURT E ,  MCMILLIAN WILLIAM A ,  CHRISTEL LEE A ,  KOVACS GREGORY T ,  NORTHRUP M ALLEN

IPC: B01J19/00 ,  G01N31/20 ,  B01D11/00 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/26 ,  C12M1/33 ,  C12M1/34 ,  C40B60/14 ,  G01N1/28 ,  G01N30/60 ,  G01N33/50 ,  G01N35/08 ,  G01N37/00

Abstract: A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g. filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41A and 41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid. A preferred embodiment relates to a device for lysing components of a fluid sample, the device comprising inter alia, an ultrasonic transducer.