公开(公告)号：HU9800526A2

公开(公告)日：1998-10-28

申请号：HU9800526

申请日：1998-03-10

Applicant: HOECHST AG

Inventor： KIRSCHBAUM BERND ,  MEISTERERNST MICHAEL ,  STAHL WILHELM ,  WINKLER IRVIN

IPC: C12N15/09 ,  C12N15/63 ,  C12P19/34 ,  C12Q1/68 ,  C12N15/11 ,  C12Q1/00

Abstract: Process for transcription of a DNA template in vitro in a cell-free system, where the template comprises a sequence to be transcribed under the control of at least one regulatory element, is characterised in that (a) an enriched and optionally purified extract of cell nuclei and at least one labelled nucleotide are used for the transcription, where the extract can optionally be supplemented or partly or completely replaced by transcription factors and/or cofactors, (b) proteins present in the reaction mixture at the end of transcription are optionally separated and/or degraded, (c) the labelled transcript is bound to a solid support, (d) excess labelled nucleotide is removed, and (e) the amount of labelled transcript is determined. Also claimed is a DNA template as above in which the sequence to be transcribed does not contain at least one nucleobase selected from G, C or T, where the G-, C- or T-free sequence has a length of more than 400 nucleotides.

公开(公告)号：TR199800430A2

公开(公告)日：1998-10-21

申请号：TR9800430

申请日：1998-03-10

Applicant: HOECHST AG

Inventor： KIRSCHBAUM BERND ,  STAHL WILHELM ,  WINKLER IRWIN ,  MEISTERERNST MICHAEL

IPC: C12N15/09 ,  C12N15/63 ,  C12P19/34 ,  C12Q1/68 ,  C12N

Abstract: Process for transcription of a DNA template in vitro in a cell-free system, where the template comprises a sequence to be transcribed under the control of at least one regulatory element, is characterised in that (a) an enriched and optionally purified extract of cell nuclei and at least one labelled nucleotide are used for the transcription, where the extract can optionally be supplemented or partly or completely replaced by transcription factors and/or cofactors, (b) proteins present in the reaction mixture at the end of transcription are optionally separated and/or degraded, (c) the labelled transcript is bound to a solid support, (d) excess labelled nucleotide is removed, and (e) the amount of labelled transcript is determined. Also claimed is a DNA template as above in which the sequence to be transcribed does not contain at least one nucleobase selected from G, C or T, where the G-, C- or T-free sequence has a length of more than 400 nucleotides.

公开(公告)号：AU4835997A

公开(公告)日：1998-06-18

申请号：AU4835997

申请日：1997-12-12

Applicant: HOECHST AG

Inventor： VERTESY LASZLO ,  KNAUF MARTIN ,  WINK JOACHIM ,  ISERT DIETER ,  STAHL WILHELM ,  RIESS GUNTHER ,  ASZODI JOZSEF ,  BELLER DOMINIQUE LE

IPC: C12P1/06 ,  A61K38/00 ,  A61K38/10 ,  A61P31/00 ,  C07K7/08 ,  C12N1/20 ,  C12P21/02 ,  C12R1/465 ,  C12R1/645 ,  A61K31/195

Abstract: The tridecapeptide of formula (I), designated 'feglymycin', and its salts and chemical equivalents are new. Mpg-Dpg-Val-Dpg-Mpg-Dpg-Mpg-Dpg-Val-Dpg-Mpg-Phe-Asp (I) Mpg = 4-hydroxyphenylglycine residue; Dpg = 3,5-dihydroxyphenylglycine residue. Also new is the microorganism Streptomyces species DSM 11171.

公开(公告)号：ES2113551T3

公开(公告)日：1998-05-01

申请号：ES93920705

申请日：1993-09-13

Applicant: BEIERSDORF AG ,  HOECHST AG

Inventor： KROPKE RAINER ,  PAPE WOLFGANG ,  SCHNEIDER GUNTHER ,  STAHL WILHELM ,  WIESNER MATTHIAS

IPC: A61K8/00 ,  A61K8/60 ,  A61K8/06 ,  A61K47/26 ,  A61Q19/00 ,  C09K23/56 ,  A61K7/50

Abstract: PCT No. PCT/EP93/02465 Sec. 371 Date Feb. 22, 1995 Sec. 102(e) Date Feb. 22, 1995 PCT Filed Sep. 13, 1993 PCT Pub. No. WO94/06408 PCT Pub. Date Mar. 31, 1994A W/O emulsion comprising water, oil and at least one water-soluble alkyl glycoside. Cosmetic and dermatological skincare composition, based thereon are easier to prepare, of lower viscosity and improved stability.

公开(公告)号：AU3423097A

公开(公告)日：1998-02-26

申请号：AU3423097

申请日：1997-08-18

Applicant: HOECHST AG

Inventor： VERTESY LASZLO ,  KURZ MICHAEL ,  WINK JOACHIM ,  MARKUS ASTRID ,  STAHL WILHELM

IPC: C12P17/18 ,  A61K31/365 ,  A61P31/04 ,  A61P31/10 ,  A61P33/02 ,  C07D493/04 ,  C07H17/08 ,  C12N1/20 ,  C12P19/62 ,  C12R1/465 ,  C07D493/08 ,  C12P17/08 ,  A61K31/71

Abstract: Polyene derivatives of formula (I-VI): their salts and obvious chemical equivalents are new. Molecular formula: (MF) C59H88N2O18 (II), molecular weight (mol. wt.) 1113.3; MF: C57H87NO18 (III), mol. wt. 1074.3; MF: C58H84N2O18 (IV), mol.wt. 1097.3; MF: C59H86N2O18 (V), mol. wt. 1111.3, and MF: C57H85NO18 (VI), mol. wt. 1072.3. X = a group of formula (a) or (b), and Y = CH=CH-CH=CH-Me or a group of formula (c) or (d). Also claimed is the micro-organism Streptomyces DSM 11007.

公开(公告)号：DE59307830D1

公开(公告)日：1998-01-22

申请号：DE59307830

申请日：1993-09-13

Applicant: BEIERSDORF AG ,  HOECHST AG

Inventor： KROEPKE RAINER ,  PAPE WOLFGANG ,  SCHNEIDER GUENTHER ,  STAHL WILHELM ,  WIESNER MATTHIAS

IPC: A61K8/60 ,  A61K8/00 ,  A61K8/06 ,  A61K47/26 ,  A61Q19/00 ,  C09K23/56 ,  A61K7/50

Abstract: PCT No. PCT/EP93/02465 Sec. 371 Date Feb. 22, 1995 Sec. 102(e) Date Feb. 22, 1995 PCT Filed Sep. 13, 1993 PCT Pub. No. WO94/06408 PCT Pub. Date Mar. 31, 1994A W/O emulsion comprising water, oil and at least one water-soluble alkyl glycoside. Cosmetic and dermatological skincare composition, based thereon are easier to prepare, of lower viscosity and improved stability.

公开(公告)号：AU672643B2

公开(公告)日：1996-10-10

申请号：AU5232893

申请日：1993-12-10

Applicant: HOECHST AG

Inventor： STAHL WILHELM ,  AHLERS MICHAEL ,  WALCH AXEL ,  BARTNIK ECKHART ,  KRETZSCHMAR GERHARD ,  GRABLEY SUSANNE ,  SCHLEYERBACH RUDOLF

IPC: A61K31/715 ,  A61K31/765 ,  A61K31/785 ,  A61K31/80 ,  A61K47/48 ,  A61K49/00 ,  A61P29/00 ,  A61P31/04 ,  A61P31/12 ,  A61P35/00 ,  A61P43/00 ,  C07H15/04 ,  C07H15/203 ,  C08B37/00 ,  C08G63/00 ,  C08G63/06 ,  C08G64/00 ,  C08G64/42 ,  C08G65/16 ,  C08G67/04 ,  C08G69/00 ,  C08G69/10 ,  C08G73/16 ,  A61K31/70 ,  C08F8/32

Abstract: The invention relates to physiologically compatible and physiologically degradable polymer-based carbohydrate receptor blocker with an HLB of 10 to 20, containing: carbohydrate portion - bifunctional spacer - hydrophilic biodegradable polymer - potentiator, where the carbohydrate portion consists of 1 to 20 naturally occurring, identical or different, monosaccharide units and is coupled via one or more bifunctional spacers, which is a natural or synthetic molecule, to a hydrophilic biodegradable polymer, and the polymer in turn is the carrier of one or more spacers and the hydrophilic biodegradable polymer is additionally linked to the potentiator which is a crosslinker, one or more groups with hydrophobic, hydrophilic or ionic properties or a solubility improver, to a method for the preparation and to its use in vivo for blocking carbohydrate-recognising receptors. The carbohydrate receptor blocker induces no incompatibility reaction in vivo either in its totality or in the form of breakdown products.

公开(公告)号：PL196578B1

公开(公告)日：2008-01-31

申请号：PL32534398

申请日：1998-03-12

Applicant: HOECHST AG

Inventor： KIRSCHBAUM BERND ,  STAHL WILHELM ,  WINKLER IRVIN ,  MEISTERERNST MICHAEL

IPC: C12N15/09 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/11 ,  C12N15/63 ,  C12P19/34

公开(公告)号：BR9704385A

公开(公告)日：1999-05-11

申请号：BR9704385

申请日：1997-08-18

Applicant: HOECHST AG

Inventor： VERTESY LASZLO ,  KURZ MICHAEL ,  WINK JOACHIM ,  MARKUS ASTRID DR ,  STAHL WILHELM

IPC: C12P17/18 ,  A61K31/365 ,  A61P31/04 ,  A61P31/10 ,  A61P33/02 ,  C07D493/04 ,  C07H17/08 ,  C12N1/20 ,  C12P19/62 ,  C12R1/465

Abstract: Polyene derivatives of formula (I-VI): their salts and obvious chemical equivalents are new. Molecular formula: (MF) C59H88N2O18 (II), molecular weight (mol. wt.) 1113.3; MF: C57H87NO18 (III), mol. wt. 1074.3; MF: C58H84N2O18 (IV), mol.wt. 1097.3; MF: C59H86N2O18 (V), mol. wt. 1111.3, and MF: C57H85NO18 (VI), mol. wt. 1072.3. X = a group of formula (a) or (b), and Y = CH=CH-CH=CH-Me or a group of formula (c) or (d). Also claimed is the micro-organism Streptomyces DSM 11007.

公开(公告)号：CA2231745A1

公开(公告)日：1998-09-12

申请号：CA2231745

申请日：1998-03-11

Applicant: HOECHST AG

Inventor： WINKLER IRVIN ,  MEISTERERNST MICHAEL ,  STAHL WILHELM ,  KIRSCHBAUM BERND

IPC: C12N15/09 ,  C12N15/63 ,  C12P19/34 ,  C12Q1/68 ,  C12N15/11

Abstract: The invention relates to an in-vitro process for analyzing transcription of vira l and cellular genes which can be automated and which is suitable for efficient and economical bulk screening with the aim of finding specific chemical lead structu res which have a selective effect on gene activity. The process for the cell-free in-vitro transcription of a DNA template comprisin g a DNA sequence to be transcribed which is under the control of at least one gene-regulatory element comprises a) using, for the transcription, a concentrated and, if appropriate, purified ex tract from cell nuclei which, if appropriate, can be complemented, or partially or ful ly replaced, by transcription factors and/or cofactors, and at least one labeled nucleotide, b) if appropriate isolating and/or degrading the proteins in the reaction mixtur e after transcription, c) binding the labeled transcript to a solid matrix, d) removing the excess labeled nucleotides and e) determining the amount of labeled transcript.