Abstract:
PURPOSE: A combined inactivated vaccine for preventing cellular diseases of fish using Streptococcus parauberis FPA4164 strain(KCTC 11980BP) is provided to effectively and cheaply prevent diseases caused by three kinds of bacteria. CONSTITUTION: An alpha-hemolytic Streptococcus parauberis FPA4164 strain(KCTC 11980BP) is isolated from flatfish. A combined inactivated vaccine for preventing cellular diseases of fish contains inactivated alpha-hemolytic Streptococcus parauberis FPA4164(KCTC 11980BP) antigen, inactivated beta-hemolytic Streptococcus iniae FP50249(KCTC 11634BP) antigen, and inactivated beta-hemolytic Edwardsiella tarda FP2018(KCTC 11145BP) antigen in a ratio of 1:1:1. The vaccine contains a montanide adjuvant. The adjuvant is montanide ISA35 or Gel01.
Abstract:
본 발명은 멍게 물렁증을 일으키는 원생동물에 관한 것이다. 또한, 본 발명은 상기 원생동물의 18S rDNA의 595 bp 단편, 이 단편의 뉴클레오티드 서열의 일부를 이용하는 멍게 물렁증 PCR 진단법 및 인시추 하이브리드화 진단법, 및 PCR 진단법 및 인시추 하이브리드화 진단법에 이용되는 프라이머 및 프로브에 관한 것이다. 멍게 물렁증, PCR 진단, 인시추 하이브리드화 진단
Abstract:
A gene of a parasite causing death of Halocynthia roretzi and a method for diagnosing a parasite are provided to obtain a living organism that causes perishing of Halocynthia roretzi, and to diagnose a parasite by using a part of 18S rDNA fraction causing perishing of Halocynthia roretzi. A method for diagnosing a parasite includes the steps of: separating the entire nucleic acid from Halocynthia roretzi; performing PCR reaction by having the separated nucleic acid as a template and having a front primer and a reverse primer having a partial sequence of 18SrDNA fraction having a nucleotide sequence of sequence number 1 as a primer pair; and checking the size of the product of the PCR reaction by gel electrophoresis and comparing the size of the aforesaids product with the size of the product that is anticipated from the used primer pair.