公开(公告)号：KR100382167B1

公开(公告)日：2003-04-26

申请号：KR1020000048358

申请日：2000-08-21

Applicant: 대한민국(농촌진흥청장)

Inventor： 황덕주 ,  허성기 ,  배신철 ,  변명옥 ,  고승주 ,  류진창

IPC: C12N15/11

Abstract: PURPOSE: Provided are gene encoding for a transcription factor which regulates the expression of defense related genes in rice and method for inducing the systemic acquired resistance by using this gene. In particular, provided is Oryza sativa ethylene response element binding protein(OsEREBP), a transcription factor which regulates the expression of defense related genes in rice. CONSTITUTION: The method of selecting OsEREBP comprises: treating the known systemic acquired resistance inducer, benzo-1,2,3-thiadiazole-7-carbothioic acid-S-methyl ether (BTH) to produce mRNA which is related to induction of systemic acquired resistance; transcribing the mRNA to obtain cDNA clone by using a transcriptase; selecting cDNA clone capable of being amplified by BTH treatment using the cDNA as a probe; and confirming a OsEREBP by analyzing nucleotide sequence and homology of cDNA clone. The nucleotide sequence of OsEREBP is represented by SEQ ID NO:1.

Abstract translation: 目的：提供了调节水稻中防御相关基因表达的转录因子的基因和通过使用该基因诱导系统获得性抗性的方法。 具体而言，提供了水稻（Oryza sativa）乙烯应答元件结合蛋白（OsEREBP），其是调节水稻中防御相关基因表达的转录因子。 构成：选择OsEREBP的方法包括：处理已知的系统获得性抗性诱导剂苯并-1,2,3-噻二唑-7-硫代羧酸-S-甲基醚（BTH）以产生与诱导系统获得性有关的mRNA 抵抗性; 通过使用转录酶转录mRNA以获得cDNA克隆; 使用该cDNA作为探针选择能够通过BTH处理扩增的cDNA克隆; 并通过分析cDNA克隆的核苷酸序列和同源性来确认OsEREBP。 OsEREBP的核苷酸序列由SEQ ID NO：1表示。

公开(公告)号：KR1020020015179A

公开(公告)日：2002-02-27

申请号：KR1020000048358

申请日：2000-08-21

Applicant: 대한민국(농촌진흥청장)

Inventor： 황덕주 ,  허성기 ,  배신철 ,  변명옥 ,  고승주 ,  류진창

IPC: C12N15/11

CPC classification number: C12N15/113 ,  C12N15/8216

Abstract: PURPOSE: Provided are gene encoding for a transcription factor which regulates the expression of defense related genes in rice and method for inducing the systemic acquired resistance by using this gene. In particular, provided is Oryza sativa ethylene response element binding protein(OsEREBP), a transcription factor which regulates the expression of defense related genes in rice. CONSTITUTION: The method of selecting OsEREBP comprises: treating the known systemic acquired resistance inducer, benzo-1,2,3-thiadiazole-7-carbothioic acid-S-methyl ether (BTH) to produce mRNA which is related to induction of systemic acquired resistance; transcribing the mRNA to obtain cDNA clone by using a transcriptase; selecting cDNA clone capable of being amplified by BTH treatment using the cDNA as a probe; and confirming a OsEREBP by analyzing nucleotide sequence and homology of cDNA clone. The nucleotide sequence of OsEREBP is represented by SEQ ID NO:1.

Abstract translation: 目的：提供调节水稻防御相关基因表达的转录因子的基因编码和使用该基因诱导系统性获得性抗性的方法。 特别地，提供了水稻乙烯应答元件结合蛋白（OsEREBP），一种调节水稻防御相关基因表达的转录因子。 构成：选择OsEREBP的方法包括：治疗已知的系统获得性阻力诱导剂，苯并1,2,3-噻二唑-7-硫代硫酸S-甲基醚（BTH），产生与诱导全身获得的mRNA相关的mRNA 抵抗性; 通过使用转录酶转录mRNA以获得cDNA克隆; 选择能够通过使用cDNA作为探针的BTH处理扩增的cDNA克隆; 并通过分析cDNA克隆的核苷酸序列和同源性来确认OsEREBP。 OsEREBP的核苷酸序列由SEQ ID NO：1表示。

公开(公告)号：KR102000454B1

公开(公告)日：2019-07-16

申请号：KR1020170137337

申请日：2017-10-23

Applicant: 대한민국(농촌진흥청장)

Inventor： 황덕주 ,  최창현 ,  안일평 ,  박상렬 ,  배신철

IPC: C12N15/82

公开(公告)号：KR101785101B1

公开(公告)日：2017-10-13

申请号：KR1020160074480

申请日：2016-06-15

Applicant: 대한민국(농촌진흥청장)

Inventor： 황덕주 ,  최창현 ,  안일평 ,  박상렬 ,  배신철

IPC: C12N15/82 ,  C12N15/113 ,  C07K14/415 ,  A01H5/00

Abstract: 본발명은벼 유래유전자인 OsDWD1(Oryza sativa DDB1 binding WD40) 유전자및 이의용도에관한것으로, 구체적으로 OsDWD1 유전자에대한 RNAi 염기서열을포함하는식물병 저항성재조합벡터를이용하여식물병 저항성을가지는형질전환식물체를제작하였으므로다양한작물에서식물병에의한피해를줄일수 있는기반기술로유용하게이용될수 있다.

Abstract translation: 本发明中，通过使用包括用于OsDWD1基因转染的RNAi序列与水稻衍生的基因OsDWD1植物病害抗性的特定植物抗病性的重组载体（稻DDB1结合WD40）基因及其应用 由于该工厂已经生产出来，它可以作为潜在的技术用于减少水瓶对各种农作物造成的损害。

公开(公告)号：KR101785095B1

公开(公告)日：2017-10-13

申请号：KR1020160148898

申请日：2016-11-09

Applicant: 대한민국(농촌진흥청장)

Inventor： 박상렬 ,  황덕주 ,  배신철 ,  안일평

IPC: C12N15/82 ,  C07K14/415

Abstract: 본발명은벼흰잎마름병저항성을증진시키는 OsGRX1 유전자를포함하는재조합벡터, 이를이용한벼흰잎마름병저항성이증진된형질전환식물체및 식물체의벼흰잎마름병저항성을증진시키는방법에관한것이다. 상기형질전환식물체를공급함으로써농약사용절감이가능하고, 그로인해생산성향상및 안전한농산물공급이가능하게될 것이다.

Abstract translation: 本发明涉及一种重组载体，使用相同的包括OsGRX1基因以促进huinip水稻白叶枯病抗性提高到增强水稻枯萎病huinip转基因植物的稻huinip白叶枯病抗性和植物用电阻的方法。 通过提供转基因植物，有可能减少杀虫剂的使用，从而提高生产力并提供安全的农产品。

公开(公告)号：KR1020170055606A

公开(公告)日：2017-05-22

申请号：KR1020150158234

申请日：2015-11-11

Applicant: 대한민국(농촌진흥청장)

Inventor： 배신철 ,  전푸름 ,  안일평 ,  박상렬 ,  황덕주 ,  김정구

IPC: C12Q1/68

Abstract: 본발명은국화흰녹병균특이적검출을위한조성물및 이를이용한국화흰녹병균의검출방법에관한것이다. 본발명의프라이머세트및/ 또는프로브를이용하는경우, 국화흰녹병균게놈 DNA를 20pg까지검출할수 있으며, 실시간 PCR을실시하는경우게놈 DNA 6pg까지검출이가능하다.

Abstract translation: 本发明涉及用于检测菊花白锈病的组合物和使用该组合物检测白锈病菌的方法。 当使用本发明的引物组和/或探针时，可以检测多达20pg的菊花基因组DNA，并且当进行实时PCR时可以检测到多达6pg的基因组DNA。

公开(公告)号：KR101696681B1

公开(公告)日：2017-01-17

申请号：KR1020150105781

申请日：2015-07-27

Applicant: 대한민국(농촌진흥청장)

Inventor： 황덕주 ,  안일평 ,  박상렬 ,  배신철 ,  이연희

IPC: C12N15/82 ,  A01H5/00 ,  A01H5/02

Abstract: 본발명은무름병저항성이증진된형질전환식물체에관한것으로, 보다구체적으로 BrWRKY7 유전자를포함하는형질전환용벡터로식물세포를형질전환하여 BrWRKY7 유전자를과발현시켜무름병에대해높은저항성을가지는형질전환식물체에관한것이다. 본발명의형질전환식물체를이용하면농약사용절감이가능하고그로인해생산성향상및 안전한농산물공급이가능하게될 것이다.

公开(公告)号：KR101677045B1

公开(公告)日：2016-11-17

申请号：KR1020140077383

申请日：2014-06-24

Applicant: 대한민국(농촌진흥청장)

Inventor： 장안철 ,  황현식 ,  이승범 ,  배신철 ,  우희종 ,  강경호 ,  김경환

IPC: C12Q1/68

Abstract: 본발명은새로운식물용해버퍼를직접중합효소연쇄반응(Direct PCR)에이용하여다양한식물체에서빠르고간단하게싱글카피삽입유전자를검정하는방법에관한것으로, 본발명의새로운식물용해버퍼와식물의내생및 외생유전자를바탕으로제작한프라이머세트를이용하여 Semi-quentitative Direct-PCR 분석을하여형질전환체 1차선발시활용한다면, 대량검정시서던하이브리디제이션(Southern Hybridization) 분석시료수를크게줄이는등 시간과비용을크게절감하는데유용하게이용할수 있을것이다.

公开(公告)号：KR1020140147519A

公开(公告)日：2014-12-30

申请号：KR1020130070929

申请日：2013-06-20

Applicant: 대한민국(농촌진흥청장)

Inventor： 안일평 ,  김동헌 ,  배신철 ,  박상렬 ,  황덕주

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/689 ,  C12N15/11 ,  C12Q1/6844 ,  C12Q1/6895

Abstract: The present invention relates to a Rhizoctonia solani AROM (RsAROM) gene-specific primer and to a method for detecting sheath blight by using the same, which can be used as a molecular biological method for diagnosing sheath blight by using a primer that can specifically detect RsAROM gene existing in a sheath blight genome and a probe to objectively quantify the development of sheath blight.

Abstract translation: 本发明涉及丝核菌属AROM（RsAROM）基因特异性引物及其用于检测皮肤枯萎病的方法，其可以用作通过使用可特异性检测的引物来诊断纹枯病的分子生物学方法 存在于纹枯病基因组中的RsAROM基因，以及客观量化纹枯病发展的探针。

公开(公告)号：KR1020120080437A

公开(公告)日：2012-07-17

申请号：KR1020110001902

申请日：2011-01-07

Applicant: 대한민국(농촌진흥청장)

Inventor： 황덕주 ,  박영호 ,  최창현 ,  안일평 ,  윤충효 ,  김민갑 ,  박상렬 ,  박홍재 ,  배신철

IPC: A01H5/00 ,  C12N15/29 ,  C12N15/82

CPC classification number: C12N15/8281 ,  C12N15/8205 ,  C12N15/8206 ,  C12N15/8209

Abstract: PURPOSE: A method for enhancing soft rot resistance in a plant is provided to reduce agrochemical usage and to improve productivity of the plants. CONSTITUTION: A method for enhancing soft rot resistance in a plant comprises a step of increasing intracellular level of RTP1(resistance to pectobacterium 1) protein having amino acids of sequence number 2 by overexpressing a gene encoding RTP1 protein. The gene has a base of sequence number 1. The gene is overexpressed by transforming a plant cell with a recombinant expression vector. The expression vector contains the gene encoding RTP1 protein or RTP1 gene.

Abstract translation: 目的：提供一种提高植物耐软腐性的方法，以减少农药的使用和提高植物的生产力。 构成：提高植物的软腐病抗性的方法包括通过过表达编码RTP1蛋白的基因增加具有序列号2的氨基酸的RTP1（具有抗原性细菌1）的蛋白质的细胞内水平的步骤。 该基因具有序列号1的碱基。该基因通过用重组表达载体转化植物细胞而过表达。 表达载体含有编码RTP1蛋白或RTP1基因的基因。