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公开(公告)号：HK1074217A1
公开(公告)日：2005-11-04
申请号：HK05106631
申请日：2005-08-02
Applicant: HOFFMANN LA ROCHE
Inventor： KRATZSCH PETER , SCHMUCK RAINER , BUNK DANIELA , SHAO ZHIXIN , THYM DETLEF , KNAPPE WOLFGANG-REINHOLD
IPC: C12N20060101 , G01N33/66 , C12M1/40 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/04 , C12N11/02 , C12N15/09 , C12N15/53 , C12P21/02 , C12Q20060101 , C12Q1/32 , C12Q1/54 , C12R20060101
Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.
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公开(公告)号：AU2003240260A1
公开(公告)日：2003-12-02
申请号：AU2003240260
申请日：2003-05-16
Applicant: HOFFMANN LA ROCHE
Inventor： HORN CARINA , HOENES JOACHIM , KNAPPE WOLFGANG-REINHOLD
IPC: C12M1/34 , C08F2/00 , C12M1/40 , C12Q1/00 , C12Q1/26 , C12Q1/32 , C12Q1/54 , G01N21/78 , G01N27/327 , G01N33/543
Abstract: A method for producing a polymer layer on a transparent support. A photopolymerizable liquid composition is applied to the support. The photopolymerizable liquid composition is irradiated through the support such that polymerization of the liquid composition starts at the surface of the support and ends at a predetermined distance over the surface of the support. Only partial polymerization of the liquid composition takes place. A polymer layer with a predetermined thickness is formed on the support wherein the predetermined thickness is less than the thickness of the photopolymerizable liquid composition. The remaining photopolymerizable liquid composition is removed from the entire polymer layer.
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公开(公告)号：BR0114962A
公开(公告)日：2003-10-28
申请号：BR0114962
申请日：2001-10-20
Applicant: HOFFMANN LA ROCHE
Inventor： KRATZSCH PETER , SCHMUCK RAINER , BUNK DANIELA , SHAO ZHIXIN , THYM DETLEF , KNAPPE WOLFGANG-REINHOLD
IPC: G01N33/66 , C12M1/40 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/04 , C12N11/02 , C12N15/09 , C12N15/53 , C12P21/02 , C12Q1/32 , C12Q1/54
Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.
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公开(公告)号：CA2486950C
公开(公告)日：2013-07-30
申请号：CA2486950
申请日：2003-05-16
Applicant: HOFFMANN LA ROCHE
Inventor： HORN CARINA , HOENES JOACHIM , KNAPPE WOLFGANG-REINHOLD
IPC: C12M1/34 , C12Q1/00 , C08F2/00 , C12M1/40 , C12Q1/26 , C12Q1/32 , C12Q1/54 , G01N21/78 , G01N27/327 , G01N33/543
Abstract: The invention relates to a method for producing polymer layers on a support by photopolymerization of a polymerizable liquid composition, to a device which is suitable for producing polymer layers on a support, and to a method for producing a sensor which comprises a polymer layer with an indicator embedded therein.
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公开(公告)号：NZ591742A
公开(公告)日：2012-12-21
申请号：NZ59174209
申请日：2009-11-06
Applicant: HOFFMANN LA ROCHE
Inventor： KNAPPE WOLFGANG-REINHOLD , HILLER BERND , LEHR URSULA , ZIMMER VOLKER , PETRICH WOLFGANG , BEDON-GOMEZ LUIS DAVID
Abstract: A diagnostic test element (110) for detecting an analyte in a sample of a bodily fluid (126). The element comprises a test field (116) with at least one detection reagent. The detection reagent is adapted to go through at least one detectable change in the presence of the analyte. The test field comprises a detection layer (118) that comprises the detection reagent and the detection layer comprises particles, wherein 90% of all the particles have a size of less than 10 micrometers. The test field has a loading side for applying the sample and the detection side for detecting a change in the detection reagent. The test field further comprises a removal layer (122) arranged on the side of the detection layer facing the loading side, wherein the removal layer comprise a pigment. The body fluid can be whole blood, the analyte can be glucose and the change in the detection reagent can be an optical change. The particles can be sodium aluminium silicate, aluminium or titanium oxide or a nanoparticulate oxidic matieral of silicon oxide, aluminium oxide and titanium oxide. The pigment can be titanium dioxide, zirconium dioxide, barium titanate, barium zirconate or zirconium silicate.
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Patent Agency Ranking
公开(公告)号：CA2570593C
公开(公告)日：2012-03-20
申请号：CA2570593
申请日：2005-07-19
Applicant: HOFFMANN LA ROCHE
Inventor： BOENITZ-DULAT MARA , LAGGERBAUER JESSICA , SCHMUCK RAINER , KRATZSCH PETER , KNAPPE WOLFGANG-REINHOLD
Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant s~GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.
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公开(公告)号：AU2003240666B2
公开(公告)日：2009-04-23
申请号：AU2003240666
申请日：2003-05-16
Applicant: HOFFMANN LA ROCHE
Inventor： HORN CARINA , HOENES JOACHIM , KNAPPE WOLFGANG-REINHOLD
IPC: C12M1/34 , C08F2/00 , C12M1/40 , C12Q1/00 , C12Q1/26 , C12Q1/32 , C12Q1/54 , G01N21/78 , G01N27/327 , G01N33/543
Abstract: A method for producing a polymer layer on a transparent support. A photopolymerizable liquid composition is applied to the support. The photopolymerizable liquid composition is irradiated through the support such that polymerization of the liquid composition starts at the surface of the support and ends at a predetermined distance over the surface of the support. Only partial polymerization of the liquid composition takes place. A polymer layer with a predetermined thickness is formed on the support wherein the predetermined thickness is less than the thickness of the photopolymerizable liquid composition. The remaining photopolymerizable liquid composition is removed from the entire polymer layer.
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公开(公告)号：NZ551870A
公开(公告)日：2009-02-28
申请号：NZ55187005
申请日：2005-07-19
Applicant: HOFFMANN LA ROCHE
Inventor： BOENITZ-DULAT MARA , LAGGERBAUER JESSICA , SCHMUCK RAINER , KRATZSCH PETER , KNAPPE WOLFGANG-REINHOLD
Abstract: Provided is a variant of PQQ-dependent soluble glucose dehydrogenase polypeptide comprising one amino acid insertion at position 428-428 of the wild type sequence from Acinetobacter calcoaceticus. Further provided are specific variants with specified amino acids in that position, and with further amino acid insertions in other specified positions. Also provided are corresponding polynucleotides, vectors coding for the variant, cells containing the vector, methods of producing the variants. The compositions and methods are useful for determining concentrations of sugars in a sample.
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公开(公告)号：HK1107829A1
公开(公告)日：2008-04-18
申请号：HK07113450
申请日：2007-12-10
Applicant: HOFFMANN LA ROCHE
Inventor： BOENITZ-DULAT MARA , LAGGERBAUER JESSICA , SCHMUCK RAINER , KRATZSCH PETER , KNAPPE WOLFGANG-REINHOLD
IPC: C12M20100101 , C12N20100101 , C12Q20100101 , G01N20100101
Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known fromto genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.
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公开(公告)号：BRPI0513691A
公开(公告)日：2008-03-25
申请号：BRPI0513691
申请日：2005-07-19
Applicant: ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE
Inventor： BOENITZ-DULAT MARA , LAGGERBAUER JESSICA , SCHMUCK RAINER , KRATZSCH PETER , KNAPPE WOLFGANG-REINHOLD
Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known fromto genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.
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