ANALYSIS CHIP AND ANALYSIS METHOD
    31.
    发明专利

    公开(公告)号:CA2671866C

    公开(公告)日:2014-03-25

    申请号:CA2671866

    申请日:2008-01-23

    Abstract: An analysis chip which has a baseboard having a selectively binding substance immobilized on the surface thereof, a covering member bonded to this baseboard and fine particles contained or injected in a movable state within a space that is formed between the baseboard and the covering member, wherein the surface of the fine particles is coated with a surfactant. Owing to this construction, it is possible to prevent the formation of bubbles inhibiting a selective reaction between a test substance and the selectively binding substance having been immobilized so that data variation and lowering in the sensitivity can be regulated and the reproducibility of the measurement can be elevated.

    METHOD OF STIRRING SOLUTION
    34.
    发明专利

    公开(公告)号:CA2559768A1

    公开(公告)日:2005-09-29

    申请号:CA2559768

    申请日:2005-03-17

    Abstract: The method of stirring a solution according to the present invention is a method of stirring a solution comprising contacting a selective binding substance immobilized on the surface of a carrier with a solution containing an analyte substance reactive with the selective binding substance, and mixing the fme particles or air bubbles into the solution containing an analyte substance, and moving the fine particles or air bubbles without allowing contact thereof with the selective binding substance-immobilized surface. The present invention provides a stirring method that accelerates the reaction of a carrier-immobilized selective binding substance with an analyte substance and detects a trace amount of analyte at high signal intensity and high S/N ratio. The present invention enables diagnosis and examination in the clinical setting by using a selective binding substance-immobilized carrier such as DNA chip.

    Soporte que tiene una sustancia de unión selectiva fijada al mismo

    公开(公告)号:ES2727785T3

    公开(公告)日:2019-10-18

    申请号:ES04733682

    申请日:2004-05-18

    Abstract: Un soporte que tiene inmovilizada una sustancia de unión selectiva, donde el soporte tiene una parte con una depresión que incluye múltiples proyecciones, la superficie del soporte comprende una resina de autofluorescencia baja que tiene una intensidad de fluorescencia de 1.000 o menos, la superficie de la resina comprende grupos carboxilo formados mediante el tratamiento de la resina con un álcali o ácido, y adicionalmente se inmoviliza una sustancia de unión selectiva sobre las superficies superiores de las múltiples proyecciones de la parte con una depresión que incluye múltiples proyecciones mediante los grupos carboxilo de la resina, donde el soporte tiene formada un área plana, y la diferencia de altura entre la superficie superior plana de las proyecciones de la parte con una depresión que incluye múltiples proyecciones y la superficie del área plana es de 50 μm o menos. donde la intensidad de fluorescencia de la resina de autofluorescencia baja se determina midiendo una placa plana limpia que tiene un grosor de 1 mm utilizando un GenePix 4000B fabricado por Axon Instruments en condiciones de una longitud de onda de excitación de 532 nm, una ganancia de fotomultiplicador fijada en 700 y una potencia de láser del 33 %.

    METHOD FOR PREPARING ARNA AND METHOD FOR ANALYSIS OF GENE EXPRESSION

    公开(公告)号:CA2795436C

    公开(公告)日:2017-12-05

    申请号:CA2795436

    申请日:2011-04-21

    Abstract: The present invention provides a method for preparing aRNA to be used for gene expression analysis from an RNA sample extracted from a tissue or cell(s) fixed with a fixative, wherein, in an amplification step of the RNA sample by reverse transcription and in vitro transcription, the ratio of aminoallyl uridine 5'-triphosphate (AA-UTP) in a nucleotide reagent used in the in vitro transcription is not less than 5 mol% and less than 25 mol% with respect to the total of uridine 5'-triphosphate (UTP) and AA-UTP.

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