公开(公告)号：KR1020050023363A

公开(公告)日：2005-03-09

申请号：KR1020050004127

申请日：2005-01-17

Applicant: (주)케비젠

Inventor： 김소미 ,  오덕근 ,  백대헌 ,  신홍식 ,  박시호 ,  이유진 ,  엄수종 ,  노영쇠 ,  박종섭

IPC: C12N1/20

CPC classification number: C12P7/6427 ,  A23C9/1234 ,  A23C11/106 ,  A23L33/12 ,  A23L33/135 ,  A23P10/30 ,  A23Y2300/59 ,  A61K35/744 ,  A61K35/745 ,  C12R1/13

Abstract: PURPOSE: A conjugated linoleic acid(CLA) producing microorganism, a capsulated composition comprising the same microorganism, and the functional food using the same composition are provided, which microorganism has improved conjugated linoleic acid(CLA) producing activity, secretes CLA into the medium and accumulates CLA within the biomass, and has tolerance against acids such as acid in the stomach and bile juice and antibiotics. CONSTITUTION: The CLA producing microorganism which converts linoleic acid(LA) into conjugated linoleic acid(CLA) is provided, wherein the CLA producing microorganism is Bifidobacterium pseudocartenulatum CBG-C4(KACC 91003, KCTC 10208BP). The capsulated composition for prevention and treatment of cancer, arteriosclerosis, diabetes and obeseness comprises the CLA producing microorganism, wherein the coating material is water-soluble polysaccharides. The functional food containing the capsulated composition is provided, wherein the food is yoghurt, cheese, kimchi and jang(fermented soybean).

Abstract translation: 目的：提供共轭亚油酸（CLA）产生微生物，包含相同微生物的包封组合物和使用相同组成的功能性食品，该微生物具有改善的共轭亚油酸（CLA）生产活性，将CLA分泌到培养基中， 在生物质内积累CLA，并且具有对胃酸，胆汁和抗生素等酸的耐受性。 构成：提供将亚油酸（LA）转化为共轭亚油酸（CLA）的CLA产生微生物，其中CLA产生微生物是假双杆菌CBG-C4（KACC 91003，KCTC 10208BP）。 用于预防和治疗癌症，动脉硬化，糖尿病和肥胖症的包封组合物包括产生CLA的微生物，其中所述涂层材料是水溶性多糖。 提供含有封装组合物的功能性食品，其中食品是酸奶，奶酪，泡菜和姜（发酵大豆）。

公开(公告)号：KR100466719B1

公开(公告)日：2005-01-17

申请号：KR1020020006318

申请日：2002-02-04

Applicant: (주)케비젠 ,  주식회사 일화

Inventor： 김동명 ,  박세호 ,  신홍식 ,  박시호

IPC: A61K9/58

Abstract: PURPOSE: Provided is a method for preparing the spherical microcapsule of the red ginseng or ginseng extract powders which is stable to the chemical reaction and non-irritative, and conceals the bitter taste of the red ginseng or ginseng by coating the red ginseng or ginseng extract powders with at least one of water soluble polymers. CONSTITUTION: The method for preparing the spherical microcapsule of the red ginseng or ginseng extract powders comprises a step for warming the solution obtained by adding sugar, water soluble starch or hydrophilic polymer to the purified water and then drying it to form the particulate solid state core seeds; a step for coating the core seeds with the red ginseng or ginseng extract powders to form the particulate of 500 to 800 micrometers, thereby preparing the rapid release type spherical microcapsule; a step for coating the rapid release type spherical microcapsule with the polymer coating solution in an amount of 20 to 40 % by total weight of coating film composition of a delayed release type spherical microcapsule to form the delayed release type spherical microcapsule of 1000 to 1200 micrometers; a step for hardening and drying the coating film of the delayed release type spherical microcapsule to a moisture content of 1 to 10 % by total weight of the coating film capsule composition; and a step for meshing the dried microcapsule to 1000 to 1200 micrometers.

Abstract translation: 目的：提供一种制备红参或人参提取物粉末的球形微胶囊的方法，该球形微胶囊对化学反应和非刺激性是稳定的，并通过涂覆红参或人参提取物来掩盖红参或人参的苦味 粉末与至少一种水溶性聚合物。 构成：制备红参或人参提取物粉末的球形微胶囊的方法包括将通过加入糖，水溶性淀粉或亲水性聚合物得到的溶液加热到纯化水中，然后干燥以形成颗粒状固体核的步骤 种子; 用红参或人参提取物粉末包覆核心种子以形成500-800微米的颗粒，由此制备快速释放型球形微囊; 以延迟释放型球状微胶囊的涂膜组合物的总重量为20至40％的量涂覆快速释放型球状微胶囊以形成1000至1200微米的缓释型球状微胶囊的步骤 ; 将所述延迟释放型球形微胶囊的涂膜硬化并干燥至所述涂膜胶囊组合物的总重量的1至10％的水分含量的步骤; 以及将干燥的微胶囊啮合至1000至1200微米的步骤。

公开(公告)号：KR100424251B1

公开(公告)日：2004-03-22

申请号：KR1020010025012

申请日：2001-05-08

Applicant: (주)케비젠 ,  신홍식

Inventor： 신홍식 ,  박명순 ,  노영쇠 ,  권윤자 ,  박시호 ,  엄수종 ,  장판식 ,  조성동

IPC: C07C227/18

Abstract: PURPOSE: A novel method for preparing α-L-aspartyl-L-phenylalanine methyl ester·HCl salt is provided, to reduce the amount of by-products, to allow the by-products to be reused after hydrolysis and to increase the production yield by 20% or more. CONSTITUTION: The method comprises the steps of reacting L-aspartic acid with trimethyl silyl chloride in the presence of an alcohol solvent to esterify the β-COOH selectively to obtain α-L-aspartyl-L-phenylalanine methyl ester·HCl. Preferably the alcohol solvent is selected from the group consisting of methyl alcohol, ethyl alcohol, allyl alcohol and benzyl alcohol. Preferably the method comprises further the steps of protecting an amine group with -CHO, Boc, benzyloxycarbonyl group, or -C(O)OR¬2 (R2 is methyl. ethyl or isobutyl group) by using acetic anhydride and formic acid.

Abstract translation: 目的：提供一种制备α-L-天冬氨酰-L-苯丙氨酸甲酯盐酸盐的新方法，以减少副产物的量，使副产物在水解后重新使用和 将产量提高20％以上。 构成：该方法包括以下步骤：在醇溶剂存在下使L-天冬氨酸与三甲基甲硅烷基氯化物反应以选择性酯化β-COOH以获得α-L-天冬氨酰-L-苯丙氨酸甲酯和amp ; middot; HCl中。 优选醇溶剂选自甲醇，乙醇，烯丙醇和苯甲醇。 优选地，该方法还包括通过使用乙酸酐和甲酸，用-CHO，Boc，苄氧基羰基或-C（O）OR 2（R 2为甲基，乙基或异丁基）保护胺基的步骤。

公开(公告)号：KR1020030081180A

公开(公告)日：2003-10-17

申请号：KR1020030023164

申请日：2003-04-12

Applicant: (주)케비젠

Inventor： 김소미 ,  오덕근 ,  백대헌 ,  신홍식 ,  박시호 ,  이유진 ,  엄수종 ,  노영쇠 ,  박종섭

IPC: C12N1/20

CPC classification number: C12P7/6427 ,  A23C9/1234 ,  A23C11/106 ,  A23L33/12 ,  A23L33/135 ,  A23P10/30 ,  A23Y2300/59 ,  A61K35/744 ,  A61K35/745 ,  C12R1/13

Abstract: PURPOSE: A novel microorganism capable of producing conjugated linoleic acid, a capsulated composition comprising the same, and a preparation method thereof are provided. The microorganism has high conjugated linoleic acid production yield and gastric juice and bile acid tolerance. CONSTITUTION: A microorganism capable of producing conjugated linoleic acid is provided, wherein the microorganism is Bifidobacterium breve CBG-C2 KACC 91001 and KCTC 10462BP, Bifidobacterium pseudocartenulatum CBG-C4 KACC 91003, and Enterococcus faecium CBG-C5 KACC 91002 and KCTC 10208BP. The capsulated composition for prevention and treatment of diseases capable of being inhibited by the conjugated linoleic acid, such as cancer, sclerosis of the arteries, diabetes and corpulence comprises the microorganism capable of producing conjugated linoleic acid.

Abstract translation: 目的：提供一种能够生产共轭亚油酸的新型微生物，包含其的包封组合物及其制备方法。 该微生物具有高共轭亚油酸产量和胃液和胆汁酸耐受性。 构成：提供能够产生共轭亚油酸的微生物，其中微生物是双歧双歧杆菌CBG-C2KACC91001和KCTC10462BP，假双歧杆菌CBG-C4KACC 91003和屎肠球菌CBG-C5 KACC 91002和KCTC 10208BP。 用于预防和治疗能够被共轭亚油酸抑制的疾病的包封组合物，例如癌症，动脉硬化，糖尿病和败血症包括能够产生共轭亚油酸的微生物。

公开(公告)号：KR1020030062126A

公开(公告)日：2003-07-23

申请号：KR1020020002561

申请日：2002-01-16

Applicant: (주)케비젠

Inventor： 엄수종 ,  장판식 ,  김소미 ,  박찬수 ,  한혜숙 ,  신원필 ,  신홍식 ,  박시호 ,  김동명

IPC: C12N15/53

CPC classification number: C12N9/0069 ,  C12P7/02 ,  C12Y113/11063

Abstract: PURPOSE: Mutant genes of human beta, beta-carotene 15, 15'-dioxygenase having improved enzyme activity are provided, so that enzyme proteins expressed from the mutant genes can be used in various fields related to the vitamin A production. CONSTITUTION: Mutant genes of human beta, beta-carotene 15, 15'-dioxygenase are produced by error-prone PCR using a human beta, beta-carotene 15, 15'-dioxygenase gene as templates, and have the enzyme activity of the expressed protein of 1100 pmol/h. A method for producing vitamin A comprises digesting beta-carotene using the expressed human beta, beta-carotene 15, 15'-dioxygenase. A method for producing vitamin A comprises transforming a host cell using the mutant genes of human beta, beta-carotene 15, 15'-dioxygenase, and culturing the transformed host cell in a medium, wherein the host cell is a plant cell, a prokaryotic cell, a yeast cell, an insect cell, a mammal cell or a poultry cell.

Abstract translation: 目的：提供具有改善的酶活性的人β，β-胡萝卜素15,15'-双加氧酶的突变基因，使得从突变基因表达的酶蛋白可用于与维生素A生产相关的各种领域。 构成：使用人β，β-胡萝卜素15,15'-双加氧酶基因作为模板，通过易错PCR产生人β，β-胡萝卜素15,15'-双加氧酶的突变基因，并具有表达的酶活性 蛋白质为1100 pmol / h。 生产维生素A的方法包括使用表达的人β，β-胡萝卜素15,15'-双加氧酶消化β-胡萝卜素。 生产维生素A的方法包括使用人β，β-胡萝卜素15,15'-双加氧酶的突变基因转化宿主细胞，并在培养基中培养转化的宿主细胞，其中宿主细胞是植物细胞，原核 细胞，酵母细胞，昆虫细胞，哺乳动物细胞或家禽细胞。

公开(公告)号：KR102216618B1

公开(公告)日：2021-02-17

申请号：KR1020190096579

申请日：2019-08-08

Applicant: (주)케비젠

Inventor： 신홍식 ,  이상왕

IPC: A23L33/105 ,  A23L33/135 ,  A23L33/17

Abstract: 본발명은아티초크를이용한유산균발효방법및 이의용도에관한것으로, 본발명의유산균을이용하여제조한아티초크발효물은종래의아티초크추출물에비해시나린의함량및 항산화활성이증진되어, 콜레스테롤저하, 간· 신장·생리대사기능개선또는숙취해소를위한건강기능식품에더욱유용하게이용될수 있다.

公开(公告)号：KR101826835B1

公开(公告)日：2018-02-08

申请号：KR1020170031142

申请日：2017-03-13

Applicant: (주)케비젠

Inventor： 신홍식 ,  이상왕 ,  백민석

IPC: C12P19/02 ,  C07H1/02

Abstract: 본발명은칼디셀룰로시럽터속 균주유래의프럭토스 1,6-비스포스페이트알돌레이즈효소를유효성분으로함유하는인산화과당유도체로부터인산화타가토스를생산하기위한조성물에관한것으로, 본발명을통해프럭토스 1,6-비스포스페이트알돌레이즈효소가인산화과당유도체인프럭토스 1-포스페이트(fructose 1-phosphate), 프럭토스 1,6-비스포스페이트(fructose 1,6-bisphosphate), 프럭토스 6-포스페이트(fructose 6-phosphate) 및화학식 1 및화학식 2로표시되는프럭토스유도체를인산화타가토스로전환하는활성을확인하였다. 따라서, 프럭토스 1,6-비스포스페이트알돌레이즈효소가기질의다양성을가지므로, 타가토스생산에대한활용도가더욱높고, 생물체로부터얻은효소를이용하므로환경친화적이며, 간단한효소고정화과정만을필요로하고, 전환수율또한높기때문에생산경비를크게줄이는한편생산효과를극대화할수 있어, 관련산업에매우유용하다.

公开(公告)号：KR101823634B1

公开(公告)日：2018-01-30

申请号：KR1020160089185

申请日：2016-07-14

Applicant: (주)케비젠 ,  신홍식

Inventor： 신홍식 ,  장승환 ,  이상왕

IPC: C12N1/20 ,  C12P7/56 ,  A23L1/30 ,  A23K10/10

Abstract: 본발명의균주는공액리놀레산을우수한효율로생성할수 있을뿐만아니라, 젖산생성능, 내산성, 내담즙성및 항생제내성을가지고있으며, 유해세균인살모넬라티피뮤리움()에대한우수한항균활성을지닌다. 또한, 본발명의균주는한국성인의분변으로부터유래하여, 섭취시장내정착성이우수하여장기능개선및 배변활동을향상시키는조성물로서이용될수 있다.

Abstract translation: 本发明的菌株不仅能够生产共轭亚油酸以良好的效率，且具有生产乳酸的能力，耐酸，胆道和抗生素抗性内，具有对抗有害细菌优异的抗菌活性，鼠伤寒沙门氏菌（）。 此外，本发明的菌株可以用作来源于韩国成人粪便的组合物，并通过改善摄入市场内的固定来改善肠道功能和改善肠道活性。

公开(公告)号：KR1020170116978A

公开(公告)日：2017-10-20

申请号：KR1020170047228

申请日：2017-04-12

Applicant: (주)케비젠

Inventor： 신홍식 ,  장승환 ,  김희정 ,  이상왕 ,  백민석 ,  백진호

IPC: C12P19/02 ,  C12N9/88 ,  C12N15/52

Abstract: 본발명은비피도박테리움롱검균주유래의프럭토스 1,6-비스포스페이트알돌레이즈효소를유효성분으로함유하는프럭토스 6-포스페이트로부터인산화타가토스를생산하기위한조성물에관한것에관한것으로, 본발명에따른프럭토스 1,6-비스포스페이트알돌레이즈효소는생물체로부터얻은효소를이용하므로환경친화적이며, 간단한효소고정화과정만을필요로하고, 종래사이코스생산과비교하여기질에있어서는단가가낮은기질을사용하고, 수율에있어서는현저하게높기때문에생산경비를크게줄이는한편생산효과를극대화할수 있는효과가있다.

Abstract translation: 本发明涉及一种组合物用于从果糖-6-磷酸含有衍生ronggeom作为活性成分的双岐杆菌菌株的果糖-1,6-二磷酸醛缩酶，本发明的制造磷酸化塔格糖 果糖-1,6-二磷酸醛缩酶是因此通过使用从生物体获得的酶，只需要一个简单的酶固定化过程中，采用在基板低成本基材与以往的生产阿洛酮糖的相比，环保 由于产量非常高，因此可以大大降低生产成本并最大化生产效果。

公开(公告)号：KR101710356B1

公开(公告)日：2017-03-08

申请号：KR1020150064736

申请日：2015-05-08

Applicant: (주)케비젠 ,  신홍식

Inventor： 신홍식 ,  장승환 ,  이상왕 ,  김희정

IPC: A23L1/30

Abstract: 본발명은멸균처리한홍삼농축액에엔테로코커스패시움() CBG-C5 균주(KACC 91002P), 락토바실러스애시도필러스() CBG-C13 균주(KACC 91980P) 및스트랩토코커스써모필러스() CBG-C19 균주(KACC 91986P)를접종한후 발효하여제조하는것을특징으로하는특정진세노사이드함량이증진된발효홍삼농축액의제조방법및 발효홍삼농축액의진세노사이드 C-K(Compound-K) 함량을증가시키는방법과상기방법으로제조된특정진세노사이드함량이증진된발효홍삼농축액및 상기발효홍삼농축액을함유하는가공식품에관한것이다.