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公开(公告)号：KR100758813B1
公开(公告)日：2007-09-19
申请号：KR1020050133381
申请日：2005-12-29
Applicant: 대한민국(농촌진흥청장)
Abstract: A method for preserving pupa infected with Cordyceps spp, entomopathogenic fungi for a long time and a method for regenerating the fungi are provided to dispense with an expensive equipment or high technology for preserving the fungi, and also to produce fruit bodies on consumer demands by taking out the preserved pupa at desired time. The cultivation time of Cordyceps spp, entomopathogenic fungi can be controlled voluntarily, thereby solving the concentration of production time and the surplus or stock of production. A long-term preserving method of pupa infected with Cordyceps spp, entomopathogenic fungi comprises: preparing the pupa infected with Cordyceps spp, entomopathogenic fungi, controlling the water content of the pupa body to 4%, and then preserving the pupa at 4°C or less, or at -70°C after freeze-drying. The fungi regenerating method comprises soaking the preserved pupa in distilled water for 1~3hr, followed by saturating the pupa with water. The infected pupa can be preserved stably in a water content of 4% at a storage temperature of 4°C or less for 135day, and the regeneration ratio is to about 97%. In addition, a preservation period is more increased at lower temperature in freezing, and is up to 1year in preserving at -70°C or less after freeze-drying.
Abstract translation: 提供了一种用于保存长时间感染冬虫夏草，昆虫病原真菌的蛹的方法和用于再生真菌的方法，以省去用于保存真菌的昂贵的设备或高技术，以及通过采取消费者需求来生产果实 在所需的时间出来保存的蛹。 冬虫夏草，昆虫病原菌的培养时间可以自愿控制，从而解决生产时间和生产过剩或库存的集中。 昆虫病原真菌感染虫蛹的长期保存方法包括：制备感染冬虫夏草，昆虫病原真菌的蛹，将蛹体的水含量控制在4％，然后在4℃保存蛹，或 较少，或在-70℃下冷冻干燥。 真菌再生方法包括将保存的蛹在蒸馏水中浸泡1〜3小时，然后用水饱和蛹。 感染蛹可以在4℃或更低的储存温度下稳定保存在4％的水含量中，135天，再生率为约97％。 另外，冷冻时的温度保持时间更长，冷冻干燥后在-70℃以下保存1年左右。
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公开(公告)号：KR100466522B1
公开(公告)日：2005-01-15
申请号：KR1020020045969
申请日：2002-08-03
Applicant: 대한민국(농촌진흥청장) , 학교법인 성균관대학
IPC: C07D209/36
Abstract: PURPOSE: Provided are an extract of Paecilomyces sp. J300 having antitumor activity and a pharmaceutical composition containing the same. The extract contains novel antitumor matters, and is thus useful for an antitumor drug composition. CONSTITUTION: A extract of Paecilomyces sp. J300 having antitumor activity contains, as antitumor matters, 5,3-dihydroxy-1-N-methyl-indoline-2-carbonylamino-alanyl-hydoxyisoleucinyl-glycine represented by the formula(1) or its derivatives. An antitumor drug composition contains one of 5,3-dihydroxy-1-N-methyl-indoline-2-carbonylamino-alanyl-hydoxyisoleucinyl-glycine or its derivatives including 5 alpha, 6 alpha-epoxy-(22E,24R)-ergosta-8(14), 22-diene-3 beta, 7 alpha-diol of the formula(2), 5 alpha, 6 alpha-epoxy-(22E, 24R)-ergosta-8, 22-diene-3 beta, 7 alpha-diol of the formula(3), ergosta-4, 6, 8(14), 22-tetraene-3-one of the formula(4) and 3 beta, 5 alpha-dihydroxy-6 beta-methoxyergosta-7, 22-diene of the formula(5).
Abstract translation: 用途：提供了拟青霉菌提取物。 具有抗肿瘤活性的J300及含有其的药物组合物。 提取物含有新的抗肿瘤物质，因此可用于抗肿瘤药物组合物。 组成：拟青霉菌提取物。 具有抗肿瘤活性的J300含有由式（1）表示的5,3-二羟基-1-N-甲基 - 二氢吲哚-2-羰基氨基 - 丙氨酰 - 羟基异黄嘌呤基甘氨酸或其衍生物作为抗肿瘤物质。 抗肿瘤药物组合物含有5,3-二羟基-1-N-甲基 - 二氢吲哚-2-羰基氨基 - 丙氨酰 - 羟基异黄嘌呤基甘氨酸或其衍生物，包括5α，6α-环氧 - （22E，24R） - 肉豆蔻酰 - 8（14），22-二烯-3β，式（2）的7α-二醇，5α，6α-环氧 - （22E，24R）-ergosta-8,22-二烯-3β，7α （3）的二醇，麦角甾-4,6,8（14），22-四烯-3-酮和3β，5α-二羟基-6β-甲氧麦角甾-7,22 （5）的二烯。
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公开(公告)号：KR1020040012399A
公开(公告)日：2004-02-11
申请号：KR1020020045969
申请日：2002-08-03
Applicant: 대한민국(농촌진흥청장) , 학교법인 성균관대학
IPC: C07D209/36
Abstract: PURPOSE: Provided are an extract of Paecilomyces sp. J300 having antitumor activity and a pharmaceutical composition containing the same. The extract contains novel antitumor matters, and is thus useful for an antitumor drug composition. CONSTITUTION: A extract of Paecilomyces sp. J300 having antitumor activity contains, as antitumor matters, 5,3-dihydroxy-1-N-methyl-indoline-2-carbonylamino-alanyl-hydoxyisoleucinyl-glycine represented by the formula(1) or its derivatives. An antitumor drug composition contains one of 5,3-dihydroxy-1-N-methyl-indoline-2-carbonylamino-alanyl-hydoxyisoleucinyl-glycine or its derivatives including 5 alpha, 6 alpha-epoxy-(22E,24R)-ergosta-8(14), 22-diene-3 beta, 7 alpha-diol of the formula(2), 5 alpha, 6 alpha-epoxy-(22E, 24R)-ergosta-8, 22-diene-3 beta, 7 alpha-diol of the formula(3), ergosta-4, 6, 8(14), 22-tetraene-3-one of the formula(4) and 3 beta, 5 alpha-dihydroxy-6 beta-methoxyergosta-7, 22-diene of the formula(5).
Abstract translation: 目的：提供拟青霉菌属 J300具有抗肿瘤活性和含有其的药物组合物。 提取物含有新的抗肿瘤物质，因此可用于抗肿瘤药物组合物。 构成：拟青霉菌提取物 具有抗肿瘤活性的J300含有作为抗肿瘤物质的由式（1）表示的5,3-二羟基-1-N-甲基 - 二氢吲哚-2-羰基氨基 - 丙氨酰 - 羟基异丁烯基 - 甘氨酸或其衍生物。 一种抗肿瘤药物组合物包含5,3-二羟基-1-N-甲基 - 二氢吲哚-2-羰基氨基 - 丙氨酰 - 羟基异亮氨酰基甘氨酸或其衍生物之一，包括5α，6α-环氧 - （22E，24R） 8（14），22-二烯-3β，7α-二醇，5α，6α-环氧 - （22E，24R） - 麦角甾-8,22-二烯-3β，7α 式（3）的二醇，式（4）的麦角胺-4,6,8（14），22-四烯-3-酮和3β，5α-二羟基-6β-甲氧麦角甾-7,22 - 二烯（式（5））。
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公开(公告)号：KR1020030012998A
公开(公告)日：2003-02-14
申请号：KR1020010047271
申请日：2001-08-06
Applicant: 대한민국(농촌진흥청장)
IPC: A01G1/04
CPC classification number: A61K36/068
Abstract: PURPOSE: A method for culturing Isaria sinclairii is provided by inoculating Isaria sinclairii to the pupa or larva of a domestic silkworm, thereby mass producing Isaria sinclairii . CONSTITUTION: The method for culturing Isaria sinclairii using a domestic silkworm comprises the steps of: inoculating spores of Isaria sinclairii to the larva or pupa of a domestic silkworm by spraying the spores on the surface of the larva or the pupa; and forming a fruit body of Isaria sinclairii from the infected pupa, wherein the inoculation process is carried out by diluting the spores of Isaria sinclairii in water in the concentration of 106/ml to 108/ml, spraying the diluted solution on the surfaces of the larvae or pupae of the domestic silkworms aged 2 weeks, 3 weeks, 4 weeks and 5 weeks, and leaving them under 95% of humidity for 12 hours; the larvae or pupae of the domestic silkworms are stored at 20 deg. C or less prior to the inoculation; and the fruit body formation process is carried out under conditions of 15 to 25 deg. C and humidity of 95%.
Abstract translation: 目的：通过将Isaria sinclairii接种到家蚕的蛹或幼虫来提供Isaria sinclairii的培养方法，从而大量生产Isaria sinclairii。 构成：使用家蚕培养伊蚊（Isaria sinclairii）的方法包括以下步骤：通过将孢子喷洒在幼虫或蛹的表面上，将Isaria sinclairii的孢子接种到家蚕的幼虫或蛹中; 并从感染的蛹中形成Isaria sinclairii的果实体，其中通过在浓度为106 / ml至108 / ml的水中稀释Isaria sinclairii的孢子进行接种过程，将稀释的溶液喷洒在 家蚕的幼虫或蛹2周，3周，4周和5周，并将其置于95％湿度下12小时; 家蚕的幼虫或蛹保存在20度。 C以下; 果实体形成处理在15〜25℃的条件下进行。 C和湿度95％。
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公开(公告)号：KR1020020067367A
公开(公告)日：2002-08-22
申请号：KR1020010007899
申请日：2001-02-16
Applicant: 대한민국(농촌진흥청장)
IPC: A01G1/04
CPC classification number: C12N1/14
Abstract: PURPOSE: Provided is a preparation method of a medium for Entomopathogenic fungi effectively and economically by using silkworm powder used as a hypoglycemic agent. The medium increases the growth rate of Entomopathogenic fungi. CONSTITUTION: The medium for Entomopathogenic fungi is characterized by dissolving 24g of silkworm powder in 1L of distilled water; sterilizing it at 121 deg.C for 15 minutes; filtering the sterilized solution with gauze and a sieve sequentially; adding 15g of agar the pure solution; sterilizing it; and pipetting 21mL of the solution into each petridish the solidifying them.
Abstract translation: 目的：通过使用用作降血糖药的蚕粉，有效和经济地提供了一种用于昆虫病原真菌的培养基的制备方法。 培养基增加了真菌致病真菌的生长速度。 构成：昆虫病原真菌的特征是将24g蚕粉溶解在1L蒸馏水中; 在121℃下灭菌15分钟; 用纱布和筛子依次过滤灭菌液; 加入15g琼脂纯溶液; 消毒; 并将21mL的溶液移液到每个头皮中固化。
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Patent Agency Ranking
公开(公告)号：KR100187897B1
公开(公告)日：1999-06-01
申请号：KR1019960062054
申请日：1996-12-05
Applicant: 대한민국(농촌진흥청장)
IPC: A01G1/04
Abstract: 본 발명은 항암·면역증강 등 각종 효능이 뛰어난 것으로 알려져 있으나 자연에서의 채집이 극히 어려운 관계로 인간에게 유용하게 이용되지 못하고 있는 동충하초(Paecilomyces japonica)를 인공재배하는 방법에 관한 것으로, 동충하초의 일종인 페실로마이세스 자포니카균 포자를 분무기를 이용하여 가잠의 4∼5령기 유충 또는 번데기 표피에 경피 접종한 후, 균사체를 형성한 번데기에서 자실체를 형성시키는 것을 특징으로 한다.
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公开(公告)号：KR1020200128946A
公开(公告)日：2020-11-17
申请号：KR1020190053163
申请日：2019-05-07
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은누에고치분말을이용한색조화장품용조성물의제조방법및 이를이용하여제조된색조화장품에관한것이다. 본발명의누에고치분말을이용한색조화장품용조성물의제조방법은, 누에고치또는정련견을준비하는제1단계, 상기준비된누에고치또는정련견에 0.01~0.5 M의수산화나트륨(NaOH) 또는염산(HCl) 용액을가하여누에고치분해물을제조하는제2단계, 상기누에고치분해물을중화하여중화물을제조하는제3단계, 상기중화물을여과하여남은물에용해되지않는고형물을얻는제4단계, 상기물에용해되지않는고형물을증류수로세척한후, 건조및 분쇄하여최종누에고치분말을제조하는제5단계및, 상기누에고치분말을포함하는색조화장품용조성물을제조하는제6단계를포함하는것이특징이다. 본발명에의해, 피부밀착력이좋아서밀림현상이없으면서, 우수한보습력및 발색력을나타내는누에고치분말을이용한색조화장품용조성물의제조방법및 이를이용하여제조된색조화장품이제공된다.
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公开(公告)号：KR101794660B1
公开(公告)日：2017-11-08
申请号：KR1020160105529
申请日：2016-08-19
Applicant: 대한민국(농촌진흥청장) , 차의과학대학교 산학협력단
CPC classification number: A61K35/64 , A23L33/10 , A23V2002/00 , A23V2200/30 , A23V2250/204 , Y10S514/925
Abstract: 본발명은견사단백질을갖는누에를함유하는위염또는소화성궤양의예방또는치료용조성물에관한것으로서, 상기견사단백질을갖는누에가포함된조성물은에탄올유도로인한위 점막궤양, 염증현상을억제하고항산화성을증가시켜, 위염또는소화성궤양의치료용조성물또는예방과개선을위한건강기능식품으로서용이하게이용될수 있다.
Abstract translation: 本发明涉及一种用于预防或治疗胃炎或含有具有丝蛋白家蚕消化性溃疡的组合物，包含具有丝蛋白家蚕的组合物抑制胃溃疡，炎症，造成引起的乙醇和抗氧化 因此可以容易地用作治疗胃炎或消化性溃疡的组合物或用作预防和改善的保健功能食品。
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公开(公告)号：KR101714545B1
公开(公告)日：2017-03-15
申请号：KR1020140079020
申请日：2014-06-26
Applicant: 대한민국(농촌진흥청장)
IPC: A61L27/22 , A61L27/38 , A61C13/107 , A61C5/00
Abstract: 본발명은골조직형성촉진용차폐막의제조방법및 이를이용하여제조된골조직형성촉진용차폐막에관한것이다.본발명의골조직형성촉진용차폐막의제조방법은, 누에고치를정련하여세리신을제거한후 실크피브로인을얻는제 1단계, 상기제 1단계에서생성된실크피브로인을염 에탄올수용액에넣고혼합하여용해시켜용해물을제조하는제2단계, 상기제 2단계에서제조된용해물에함유된염과에탄올을제거하여염과에탄올이제거된실크피브로인수용액을제조하는제3단계, 상기제 3단계에서제조된상기실크피브로인수용액에골조직생성촉진효과를갖는테트라사이클린에탄올용액을넣고혼합하여차폐막조성물을제조하는제4단계및, 상기제4단계에서제조된차폐막조성물을평평한용기에붓고건조하여테트라사이클린 5중량%이상~10중량%미만과나머지실크피브로인으로이루어지는골조직형성촉진용차폐막을제조하는제5단계를포함하며, 실크피브로인단독차폐막의골조직형성능대비더 촉진된골조직형성능을나타내며, 대장균또는화농균에대하여항균효과를나타내는것이특징이다.본발명에의해, 항균효과를갖으면서우수한골조직형성촉진효과를갖는차폐막의제조방법및 이를이용하여제조된차폐막이제공된다.
Abstract translation: 本发明涉及一种用于促进骨质疏松的阻隔膜的制备方法，以及通过使用该阻挡膜促进骨质疏松的方法。 用于促进骨质疏松的阻隔性膜组合物的制造方法包括以下步骤：通过从茧中除去丝胶蛋白来获得丝素蛋白的第一步骤; 将盐和乙醇水溶液放入丝素蛋白中然后混合，以便通过溶解丝素蛋白产生裂解物的第二步骤; 通过除去裂解物中所含的盐和乙醇来生产水性丝素蛋白溶液的第三步骤; 以及将四环素放入含水丝素蛋白溶液中的第四步骤，使得四环素的含量相对于阻隔膜组合物的总重量可以为5-10重量％，然后将其混合，以产生 阻隔膜组成。 根据本发明，提供了具有抗菌效果和优异的骨促进效果的阻隔膜的制造方法。 此外，提供了通过使用其制造的阻隔膜。
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公开(公告)号：KR101582172B1
公开(公告)日：2016-01-06
申请号：KR1020120112845
申请日：2012-10-11
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은 PCR 방법을이용하여밀리타리스동충하초를동정하는방법, 상기방법에사용되는프라이머및 상기프라이머를포함하는밀리타리스동충하초동정용키트에관한것이다. 본발명의방법을이용하면, 외관상으로용이하게구별되지않는밀리타리스동충하초를정확하고신속하게동정할수 있으므로, 밀리타리스동충하초를포함하는다양한제품의개발에널리활용될수 있을것이다.
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