公开(公告)号：KR1020140086235A

公开(公告)日：2014-07-08

申请号：KR1020120156472

申请日：2012-12-28

Applicant: 성균관대학교산학협력단

Inventor： 이석찬 ,  변희성 ,  길의준 ,  조상호 ,  이민지 ,  박정안 ,  김재덕 ,  최홍수 ,  곽해련 ,  김미경

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q2527/101 ,  C12Q2563/107 ,  C12Q2565/125

Abstract: The present invention relates to a set of four primers for isothermal amplification reaction for detecting Squash mosaic virus, a composition including the same, and a method for detecting the Squash mosaic virus using the composition. By using the primer set, all five mutants of the Squash mosaic virus can be effectively detected without a professional device in a short time using the isothermal amplification method. In addition, the Squash mosaic virus can be rapidly diagnosed with a naked eye under natural light using highly concentrated SYBR Green I. Therefore, the present invention facilitates early detection of virus which harms farms cultivating crops such as squashes, melons, water melons, and the like, thereby making it possible to rapidly and efficiently construct a system for diagnosing the Squash mosaic virus.

Abstract translation: 本发明涉及用于检测壁球花叶病毒的等温扩增反应的四个引物，包含其的组合物以及使用该组合物检测南瓜花叶病毒的方法。 通过使用引物组，使用等温扩增方法可以在短时间内无需专业设备有效地检测南瓜花叶病毒的所有五种突变体。 另外，使用高度浓缩的SYBR Green I，可以在自然光下用肉眼快速诊断壁球花叶病毒。因此，本发明有助于早期检测危害农作物的种植物，如农作物，如南瓜，瓜，西瓜和 从而能够快速有效地构建用于诊断壁球花叶病毒的系统。

公开(公告)号：KR1020140086234A

公开(公告)日：2014-07-08

申请号：KR1020120156471

申请日：2012-12-28

Applicant: 성균관대학교산학협력단

Inventor： 이석찬 ,  변희성 ,  길의준 ,  조상호 ,  이민지 ,  박정안 ,  김재덕 ,  최홍수 ,  김미경 ,  곽해련

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q2527/101 ,  C12Q2563/107 ,  C12Q2565/125

Abstract: The present invention relates to a set of four primers for isothermal amplification reaction for detecting Cucumber mosaic virus, a composition including the same, and a method for detecting the Cucumber mosaic virus using the composition. By using the primer set, all five mutants of the Cucumber mosaic virus can be effectively detected without a professional device in a short time using the isothermal amplification method. In addition, the Cucumber mosaic virus can be rapidly diagnosed with a naked eye under natural light using highly concentrated SYBR Green I. Therefore, the present invention facilitates early detection of virus which harms farms cultivating crops such as cucumbers, water melons, hot peppers, and the like, thereby making it possible to rapidly and efficiently construct a system for diagnosing the Cucumber mosaic virus.

Abstract translation: 本发明涉及用于检测黄瓜花叶病毒的等温扩增反应的四个引物，包含其的组合物以及使用该组合物检测黄瓜花叶病毒的方法。 通过使用引物组，使用等温扩增方法可以在短时间内无需专业设备有效地检测黄瓜花叶病毒的所有5种突变体。 此外，使用高度浓缩的SYBR Green I，可以在自然光下用肉眼快速诊断黄瓜花叶病毒。因此，本发明有助于早期检测危害农作物的培养，例如黄瓜，西瓜，辣椒， 从而能够快速有效地构建用于诊断黄瓜花叶病毒的系统。

公开(公告)号：KR101402464B1

公开(公告)日：2014-06-03

申请号：KR1020120115652

申请日：2012-10-17

Applicant: 성균관대학교산학협력단

Inventor： 이석찬 ,  박정안 ,  정유철

IPC: C12Q1/68 ,  C12N15/11

Abstract: 본 발명은 국화황화모틀바이로이드(Chrysanthemum chlorotic mottle viroids, CChMVd)의 변이주(Strain)를 중합효소연쇄반응 및 제한효소 절편길이 다형성(Polymerase chain reaction-Restriction fragment length polymorphism, PCR-RFLP)법을 이용하여 구별하는 방법에 관한 것이다. 본 발명에 따른 방법을 이용하면 효과적으로 국화황화모틀바이로이드의 5개 변이주인 msim34, SSHA6, Beijing, India 669541, India 646404를 구별할 수 있다. 따라서 국화재배농가에 막대한 피해를 주고 있는 국화 감염성 바이로이드를 조기에 신속하고 정확하게 검출 가능하게 하여, 막대한 경제 손실을 현저히 감소시킬 수 있을 것으로 기대된다.

公开(公告)号：KR1020140048002A

公开(公告)日：2014-04-23

申请号：KR1020120114456

申请日：2012-10-15

Applicant: 성균관대학교산학협력단

Inventor： 이석찬 ,  강솔 ,  박정안 ,  길의준

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/04 ,  C12Q2527/101

Abstract: The present invention relates to a primer set for an isothermal amplification reaction to detect Chrysanthemum stunt viroid (CSVd), a composition comprising the primer set, and a method for detecting CSVd using the composition. When the primer set for an isothermal amplification reaction to detect CSVd according to the present invention is used, the CSVd can be effectively detected without technical equipment on the spot for a short period of time. In addition, viroids can be detected even with a small amount of sample, and the diagnostic results can be promptly verified by the naked eye under the natural light by using a high-concentration of SYBR Green I. Therefore, Chrysanthemum infectious viroids that seriously damage Chrysanthemum farmhouses can be detected early, promptly, and accurately, thereby significantly reducing a huge economic loss due to the Chrysanthemum infectious viroids.

Abstract translation: 本发明涉及用于检测菊花特发性类病毒（CSVd）的等温扩增反应的引物组，包含引物组的组合物，以及使用该组合物检测CSVd的方法。 当使用根据本发明的用于检测CSVd的等温扩增反应的引物时，可以在没有技术设备的情况下，在现场短时间内有效地检测CSVd。 此外，即使使用少量的样品也能够检测到病毒，并且通过使用高浓度的SYBR Green I，可以在自然光下通过肉眼迅速验证诊断结果。因此，严重损害菊花感染性病毒 可以及早，准确地检测到菊花农舍，从而大大减少了菊花感染性病毒的巨大经济损失。

公开(公告)号：KR1020130123669A

公开(公告)日：2013-11-13

申请号：KR1020120046970

申请日：2012-05-03

Applicant: 성균관대학교산학협력단

Inventor： 이석찬 ,  길의준 ,  박정안

IPC: A01H4/00 ,  C12N15/82

Abstract: The present invention relates to viral genome sequences of tomato yellow leaf curl virus Korea isolate DNA-A inducing leaf curling, honeysuckle yellow vein mosaic virus Korea isolate beta-satellite DNA, and a recombinant plasmid including the same. The present invention can induce virus infection in various plant bodies and can be applied to various studies including the prevention of virus by using the various plant bodies.

Abstract translation: 本发明涉及番茄黄叶卷曲病毒韩国分离株DNA-A诱导叶卷曲，金银花黄色静脉花叶病毒韩国分离株β卫星DNA的病毒基因组序列及其重组质粒。 本发明可以诱导各种植物体内的病毒感染，可以应用于各种研究，包括通过使用各种植物体来预防病毒。

公开(公告)号：KR1020130049670A

公开(公告)日：2013-05-14

申请号：KR1020110114820

申请日：2011-11-04

Applicant: 성균관대학교산학협력단

Inventor： 이석찬 ,  정유철 ,  박정안

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/04 ,  C12Q2527/101 ,  C12Q2563/107

Abstract: PURPOSE: A primer composition for loop-mediated isothermal amplification for detecting Chrysanthemum chlorotic mottle viroid, and a use thereof are provided to quickly diagnose using high concentration SYBR Green I with naked eye under natural light. CONSTITUTION: A primer set for loop-mediated isothermal amplification for detecting Chrysanthemum chlorotic mottle viroid contains sequence in sequence numbers 1-4. The Chrysanthemum chlorotic mottle viroid is SSHA6 strains or msim34 strains. A primer composition for loop-mediated isothermal amplification for detecting Chrysanthemum chlorotic mottle viroid contains the primer set. The composition further contains DNA polymerase, dNTPs, and a reaction buffer.

Abstract translation: 目的：用于环路介导的等温扩增用于检测菊花褪绿斑马疹的底漆组合物，并提供其用途，在自然光下用肉眼快速诊断高浓度SYBR Green I。 构成：用于检测菊花褪绿斑马疹的环介导等温扩增的引物组包含序列号1-4中的序列。 菊花褪绿斑马疹是SSHA6菌株或msim34菌株。 用于检测菊花褪绿斑驳病毒的环介导等温扩增的底漆组合物含有引物组。 该组合物还含有DNA聚合酶，dNTP和反应缓冲液。

公开(公告)号：KR1020120043924A

公开(公告)日：2012-05-07

申请号：KR1020100105222

申请日：2010-10-27

Applicant: 성균관대학교산학협력단

Inventor： 이석찬 ,  박정안 ,  이혜정 ,  최홍수

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/701 ,  C12Q1/6844

Abstract: PURPOSE: A primer set for detecting begomovirus is provided to accurately and quickly diagnose begomovirus infection from plant samples. CONSTITUTION: A primer set for detecting begomovirus comprises one or more kinds of primer sets selected among a primer set of: sequence numbers 1 and 2; sequence numbers 3 and 4; sequence numbers 5 and 6; sequence number 7 and 8; sequence numbers 9 and 10; sequence number 11 and 12; sequence numbers 13 and 14; sequence number 15 and 16; sequence numbers 17 and 18; and sequence numbers 19 and 20. The begomovirus includes tomato leaf curl virus, tomato yellow leaf curl virus, tobacco leaf curl virus, honeysuckle yellow vein virus, or sweet potato leaf curl virus.

Abstract translation: 目的：提供用于检测青霉素病毒的引物组，以准确快速地诊断植物样品中的胚胎病毒感染。 构成：用于检测霉菌病毒的引物组包括选自序列号1和2的引物组中的一种或多种引物组; 序列号3和4; 序列号5和6; 序列号7和8; 序列号9和10; 序列号11和12; 序列号13和14; 序列号15和16; 序列号17和18; 序列号19和20。枯叶病毒包括番茄叶卷曲病毒，番茄黄叶卷曲病毒，烟叶卷曲病毒，金银花黄色静脉病毒或甘薯叶卷曲病毒。

公开(公告)号：KR1020090112597A

公开(公告)日：2009-10-28

申请号：KR1020090035785

申请日：2009-04-24

Applicant: 성균관대학교산학협력단 ,  대한민국(농촌진흥청장)

Inventor： 이석찬 ,  이건섭 ,  최은석 ,  이용 ,  최홍수 ,  곽해련 ,  김미경 ,  이수헌 ,  김정수 ,  박진우 ,  정미남 ,  고숙주 ,  김국형 ,  박정안

IPC: C12N15/34 ,  C12N15/63

Abstract: PURPOSE: A gene which is derived from sweet potato leaf curl infection DNA virus with an activity of suppressing gene silence after transcription is provided to obtain AC2 gene with sequence of the sequence number 16. CONSTITUTION: A gene derived from a sweet potato leaf curl infection DNA virus with an activity of suppressing gene silence after transcription is AC2 gene having sequence of the sequence number 16. An AC2 protein derived from a sweet potato leaf curl infection DNA virus is denoted by the sequence of the sequence number 17.

Abstract translation: 目的：提供具有抑制转录后基因沉默活性的甘薯叶卷曲感染DNA病毒的基因，以获得序列号为16的序列的AC2基因。构成：源自甘薯叶卷曲感染的基因 具有抑制转录后基因沉默的活性的DNA病毒是具有序列号16的序列的AC2基因。来自甘薯叶卷曲感染DNA病毒的AC2蛋白由序列号17的序列表示。