公开(公告)号：KR101309061B1

公开(公告)日：2013-10-15

申请号：KR1020100076936

申请日：2010-08-10

Applicant: 전북대학교산학협력단

Inventor： 김대혁 ,  양문식 ,  장용석 ,  김정미

IPC: C12N9/02 ,  C12N15/81 ,  C12N1/19 ,  C12R1/865

Abstract: 본 발명은 신규한 라카제 단백질 및 이의 용도에 관한 것으로, 보다 자세하게는 탄닌산을 분해하는 크리포넥트리아 파라시티카(
Cryphonectria parasitica ) 유래의 라카제 3, 이의 코딩 영역을 포함하는 재조합 발현 벡터, 이 재조합 발현 벡터로 형질전환된 효모 숙주세포, 이 숙주세포를 배양하여 라카제 3을 제조하는 방법 및 라카제 3의 용도에 관한 것이다.
본 발명에 의하면, 탄닌산을 분해하는 라카제 3을 효소 활성이 유지되는 상태로 대량 생산할 수 있다. 본 발명의 라카제 3은 펄프, 식품, 섬유 산업 등 다양한 분야에서 활용될 수 있다.

公开(公告)号：KR1020130041574A

公开(公告)日：2013-04-25

申请号：KR1020110105919

申请日：2011-10-17

Applicant: 전북대학교산학협력단

Inventor： 양문식 ,  김태금 ,  김미영

IPC: C12N15/33 ,  C12N15/82 ,  A01H5/00 ,  A61P31/12

Abstract: PURPOSE: A method for manufacturing a recombinant dengue virus vaccine using a plant is provided to produce a large amount of safety vaccines. CONSTITUTION: A recombinant vector contains a synthetic gene, scED III(synthetic consensus dengue virus envelope protein domain III) with a base of sequence number 1. The recombinant vector sequentially comprises: a rice plant amylase D3(RAmy3D), RAmy3D signal peptide coding sequence, scED III synthetic gene, and a sequence encoding endoplasmic reticulum retention sequence(SEKDEL). A method for producing a large amount of scED III proteins in a plant comprises a step of transducing the recombinant vector into a plant and overexpressing scED III synthetic gene.

Abstract translation: 目的：提供一种使用植物制造重组登革热病毒疫苗的方法，以生产大量的安全性疫苗。 构成：重组载体含有具有序列号1的碱基的合成基因scED III（合成共有登革热病毒包膜蛋白结构域III）。重组载体依次包含：水稻植物淀粉酶D3（RAmy3D），RAmy3D信号肽编码序列 ，scED III合成基因和编码内质网保留序列（SEKDEL）的序列。 用于在植物中产生大量scED III蛋白质的方法包括将重组载体转导到植物中并过度表达scED III合成基因的步骤。

公开(公告)号：KR1020120138994A

公开(公告)日：2012-12-27

申请号：KR1020110058488

申请日：2011-06-16

Applicant: 전북대학교산학협력단

Inventor： 김대혁 ,  양문식 ,  장용석

IPC: C12N1/15 ,  C12P1/02 ,  C12Q1/02

Abstract: PURPOSE: A mutant strain which produces a large amount of phleichrome is provided to effectively produce phleichrome. CONSTITUTION: A mutant Cladosporium phlei strain has improved phleichrome productivity. The mutant is prepared by irradiating with UV ray at 3.0 J/cm^2 to 6 J/cm^2 for 10 to 20 seconds. The mutant strain is Cladosporium phlei M0035(deposit number: KCTC 11935BP). The mutant is cultured in a solid medium or V8 juice agar.

Abstract translation: 目的：提供产生大量phleichrome的突变菌株以有效地产生phleichrome。 构成：突变体Cladosporium phlei菌株具有提高的肉鸡生产力。 通过以3.0J / cm 2至6J / cm 2的UV射线照射10至20秒来制备突变体。 突变菌株是Cladosporium phlei M0035（保藏号：KCTC 11935BP）。 将该突变体在固体培养基或V8果汁琼脂中培养。

公开(公告)号：KR1020120052794A

公开(公告)日：2012-05-24

申请号：KR1020100114104

申请日：2010-11-16

Applicant: 전북대학교산학협력단 ,  재단법인 전주농생명소재연구원 ,  (주)엔비엠

Inventor： 양문식 ,  권태호 ,  신윤지

IPC: C12N15/82 ,  A01H5/00 ,  C12N15/57 ,  C12N9/76

Abstract: PURPOSE: A recombinant vector for Oriza sativa transformation and a method for preparing bovine trypsin using the same are provided to obtain a large amount of trypsin through a simple process. CONSTITUTION: A recombinant vector for Oriza sativa transformation comprises an Oriza sativa-derived alpha-amylase 3D(RAmy3D) promoter and a gene encoding bovine trypsin which is operatively linked in a transcriptional direction. The promoter and gene has a base sequence of sequence numbers 1 and 2. The recombinant vector is Pmyt111. A transgenic Oriza sativa is prepared by the recombinant vector. A method for preparing a large amount of bovine trypsin comprises: a step of constructing the recombinant vector; a step of transforming the recombinant vector to Oriza sativa callus; and a step of culturing the callus.

Abstract translation: 目的：提供用于转基因植物的重组载体和使用其的牛胰蛋白酶的制备方法，以通过简单的方法获得大量的胰蛋白酶。 构成：用于苜蓿苜蓿转化的重组载体包含渥太华苜蓿来源的α-淀粉酶3D（RAmy3D）启动子和编码牛胰蛋白酶的基因，其在转录方向上可操作地连接。 启动子和基因具有序列号1和2的碱基序列。重组载体是Pmyt111。 通过重组载体制备转基因玉米螟。 制备大量牛胰蛋白酶的方法包括：构建重组载体的步骤; 将重组载体转化到Oriza sativa愈伤组织的步骤; 和培养愈伤组织的步骤。

公开(公告)号：KR1020120014766A

公开(公告)日：2012-02-20

申请号：KR1020100076936

申请日：2010-08-10

Applicant: 전북대학교산학협력단

Inventor： 김대혁 ,  양문식 ,  장용석 ,  김정미

IPC: C12N9/02 ,  C12N15/81 ,  C12N1/19 ,  C12R1/865

Abstract: PURPOSE: A novel laccase protein and a method for preparing the same are provided to be used in pulp, food, and fiber industries. CONSTITUTION: A laccase 3 which decomposes tannic acid is isolated from Cryphonectria parasitica. The laccase 3 has an amino acid of sequence number 7. A recombinant expression vector contains a region encoding laccase 3. The region is a region encoding mature laccase 3 and is regulated by inducible promoter. The inducible promoter is GAL1(galactose kinase) promoter. The recombinant expression vector is pYEGLAC3 or pYEGALLAC3. A yest host cell transformed by the recombinant expression vector is Saccharomyces sp.

Abstract translation: 目的：提供一种新型漆酶蛋白及其制备方法，用于纸浆，食品和纤维行业。 构成：分离鞣酸的漆酶3从寄生菌中分离出来。 漆酶3具有序列号7的氨基酸。重组表达载体含有编码漆酶3的区域。该区域是编码成熟漆酶3的区域，并由诱导型启动子调节。 诱导型启动子是GAL1（半乳糖激酶）启动子。 重组表达载体是pYEGLAC3或pYEGALLAC3。 由重组表达载体转化的最后宿主细胞是酵母属（Saccharomyces sp。）。

公开(公告)号：KR1020110091332A

公开(公告)日：2011-08-11

申请号：KR1020100011115

申请日：2010-02-05

Applicant: 전북대학교산학협력단

Inventor： 양문식

IPC: C12N15/82 ,  C12N15/31 ,  A01H5/00 ,  A61K39/108

CPC classification number: Y02A50/474

Abstract: PURPOSE: A plant culture which is transformed with an E.coli enterotoxin B subunit gene is provided to enable oral administration in a digestive organ. CONSTITUTION: An expression vector contains E.coli enteritoxin B subunit gene between rice amylase 1A promoter and 3'-untranslated region. A hygromycin phosphotransferase gene is coated as a selection marker. The subunit gene is denoted by sequence number 1. A vaccine composition for preventing diarrhea contains a plant culture transformed by a gene encoding the B subunit or plant culture extract. A food additive for preventing diarrhea contains the plant culture. The vaccine composition is used for oral administration.

Abstract translation: 目的：提供用大肠杆菌肠毒素B亚基基因转化的植物培养物，以便在消化器官中口服给药。 构成：表达载体含有大米淀粉酶1A启动子和3'非翻译区之间的大肠杆菌肠毒素B亚基基因。 将潮霉素磷酸转移酶基因作为选择标记进行包被。 亚基基因由序列号1表示。用于预防腹泻的疫苗组合物含有由编码B亚基或植物培养物提取物的基因转化的植物培养物。 用于预防腹泻的食品添加剂含有植物培养物。 疫苗组合物用于口服给药。

公开(公告)号：KR100610280B1

公开(公告)日：2006-10-17

申请号：KR1020030074709

申请日：2003-10-24

Applicant: 전북대학교산학협력단

Inventor： 양문식 ,  강태진

IPC: C12N15/31

Abstract: 본 발명은 개시코돈을 구성하는 최초 염기에는 상방향으로 코작서열이 결합되고 개시코돈 이후의 최초 코돈의 처음염기는 G로 하며, 종결코돈의 하방향에 소포체보유신호를 코딩하는 서열이 부착되며, 코돈 선택에 있어서 당해 식물체에서 사용되는 코돈 사용에 따라 일부 또는 전체가 변형된 콜레라톡신 B서브유닛(CTB)을 코딩하는 유전자를 제공한다. 상기 유전자는 식물체로의 형질전환시에 CTB 발현정도를 크게 향상시키고, 또한 식물체내로의 형질전환시에 얻어지는 CTB가 활성의 펜타머 구조를 이루게 한다.
CTB

公开(公告)号：KR1020030014976A

公开(公告)日：2003-02-20

申请号：KR1020010048982

申请日：2001-08-14

Applicant: 전북대학교산학협력단

Inventor： 이재화 ,  권태호 ,  양문식

IPC: C12N5/14

CPC classification number: C12P21/02 ,  C07K14/535 ,  C12N5/0025 ,  C12N15/8257 ,  C12N2501/998 ,  C12N2510/00 ,  C12N2510/02

Abstract: PURPOSE: A method for mass-producing recombinant proteins using plant cells is provided, thereby cheaply mass-producing human granulocyte macrophage colony stimulating factors(hGM-CSF). CONSTITUTION: The method for mass-producing recombinant proteins using plant cells is characterized by adding at least one polymeric compound selected from the group consisting of gelatin, polyethylene glycol(PEG) and polyvinylpyrrolidine(PVP) into a medium for culturing a transformant, wherein the transformant is capable of expressing human granulocyte macrophage colony stimulating factor(hGM-CSF); the polymeric compound is added in an amount of 0.5 to 5 wt.%; the polymeric compound is added within 6 days of the cultivation of the transformant; and the transformant is Nicotiana tabacum pMYO64(KCTC 0670BP).

Abstract translation: 目的：提供使用植物细胞大规模生产重组蛋白的方法，从而廉价地批量生产人粒细胞巨噬细胞集落刺激因子（hGM-CSF）。 构成：使用植物细胞大量生产重组蛋白质的方法的特征在于将至少一种选自明胶，聚乙二醇（PEG）和聚乙烯吡咯烷酮（PVP）的聚合物）加入培养转化体的培养基中，其中 转化体能够表达人粒细胞巨噬细胞集落刺激因子（hGM-CSF）; 以0.5〜5重量％的量加入高分子化合物; 在培养转化体后6天内加入高分子化合物; 并且转化体是烟草pMYO64（KCTC 0670BP）。