Abstract:
본발명은양이온성고분자와전단응력을이용한미세조류로부터지질의추출방법및 장치에관한것으로, 보다상세하게는미세조류의건조과정없이, 음전하를띠고있는미세조류의세포벽을파괴할수 있도록, 말단에양전하기와지방족사슬을포함하는고분자와교반또는통기에의해발생되는전단응력(Shear stress)을이용한미세조류로부터지질의추출방법및 장치에관한것이다. 본발명에따른미세조류로부터지질의추출방법및 장치이용은미세조류의건조및 에너지투입없이, 미세조류로부터지질을추출할수 있으므로, 바이오디젤의에너지및 생산단가절감에효과가있다. 또한, 처리과정에서소모되는화학물질없이양이온고분자코팅과미세조류세포벽사이의상호작용및 전단응력만으로처리가이루어지므로친환경적인바이오디젤하부공정을실현할수 있다. 또한, 양이온고분자만반응시켰을때 보다전단응력까지포함된미세조류연속반응기를사용한경우, 전단응력과전하간의상호작용시너지효과에의해미세조류로부터지질의추출시간을단축할수 있고, 높은추출수율을얻을수 있다.
Abstract:
The present invention relates to a cultivation method of microalgae, which comprises a step of forming a polymer thin film, a step of pre-treating wastewater using the polymer thin film, and a step of cultivating microalgae using the pre-treated wastewater as a culture medium. If wastewater is pre-treated with a cationic polymer in accordance to the present invention, the bacterial population in the wastewater decreases, which is able to increase the productivity of microalgae, and to effectively remove contaminants in the wastewater.
Abstract:
The present invention relates to a method for producing a protein chip capable of using, as supports, various materials such as paper, cloth, and the like, and including a kringle domain variant, and more specifically, to a method for producing a protein chip, comprising the steps of: coating a water repellent polymer on a support through initiated chemical vapor deposition (iCVD); coating a polymer having a vinyl group on the support coated with the water repellent polymer; and immobilizing a complex of a kringle domain variant having a binding capacity specific to a target molecule and an amino acid residue including a thiol group on the support. Further, the present invention provides a protein chip produced by the method and a method for diagnosing a disease by using the protein chip. According to the present invention, the materials which are cheap and easy to carry can be used as a support for the protein chip. The kringle domain variant which has excellent solubility, thermal stability, and productivity and is cheap can be used as an antibody. Further, the binding of the thiol group and the vinyl group is applied to the production of the protein chip, thereby improving the reactivity between the kringle domain variant and the target molecule, so that a protein chip having improved portability and reactivity at low costs as compared with the existing protein chip. [Reference numerals] (AA,CC) DR5 concentration (쨉M); (BB) Signal intensity
Abstract:
The present invention comprises a step for providing a monomer and an initiator between a first surface and a second surface which are faced to each other; a step for forming free radical by performing thermal decomposition for the initiator by injecting thermal; a step for activating the monomer by using the free radical and forming a high molecule by performing a chain polymerase reaction with the monomer; a step for forming a high molecule thin film by enabling the high molecule to be deposited on the first surface and the second surface which are faced to each other; and a step for offering a combined material having two or more functional groups connected to a specific functional group between high molecule thin films positioned on each surface and for forming an adhesive thin film by combining the combined material with a plurality of high molecules positioned on the first surface and the second surface. [Reference numerals] (1) Decompose an initiator; (2) Firstly activate a radical and a monomer; (3) Form a polymer on a surface; (AA) Monomer and the initiator; (BB) Heated filament