公开(公告)号：JPS624898A

公开(公告)日：1987-01-10

申请号：JP14438085

申请日：1985-07-01

Applicant: AGENCY IND SCIENCE TECHN

Inventor： MIZUTANI FUMIO ,  IIJIMA SEIICHIRO ,  TANABE GIICHI ,  TSUDA KEISHIRO

IPC: C25B13/08 ,  B01J35/02 ,  C25B11/04 ,  C25D13/08 ,  C25D13/20 ,  H01M4/86

Abstract: PURPOSE:To efficiently produce an electrically conductive polymer film contg. particles by dispersing particles in a generally used insulating polymer film and combining the polymer film with an electrolytically polymerized polymer to convert the polymer film into an electric conductor. CONSTITUTION:An insulating polymer film contg. dispersed particles is formed on the surface of a substrate electrode. This coated electrode as a working electrode is put in an electrolyte soln. contg. a monomer capable of forming an electrically conductive polymer film by electrolytic polymn. on the surface of an uncoated substrate electrode used as a working electrode. Electric current is then supplied to carry out electrolytic polymn. The polymer film as a dispersion medium for the solid particles is converted into an electric conductor and an electrically conductive polymer film contg. particles is produced.

公开(公告)号：JPS63206658A

公开(公告)日：1988-08-25

申请号：JP3995387

申请日：1987-02-23

Applicant: AGENCY IND SCIENCE TECHN

Inventor： YOKOYAMA NAOKI ,  NAKAO YUKIMICHI ,  OHASHI SHINICHI ,  KAERIYAMA KYOJI ,  TSUDA KEISHIRO ,  SUDA MASAO

IPC: G01N33/68

Abstract: PURPOSE:To safely and economically detect the protein immobilized on a membrane with high sensitivity, by adsorbing palladium colloid by protein and applying the electroless plating of a base metal to said protein to sensitize the same. CONSTITUTION:In detecting the protein immobilized on a membrane, this membrane is treated with palladium colloid stabilized with a nonionic surfactant. As the membrane used in the immobilization of protein to be detected, any one commonly used in a gold colloid method is used. As a method for immobilizing protein to be inspected on the membrane, for example, an electroblotting method is used. Subsequently, the electroless plating of a base metal is further applied to the membrane thus treated to sensitize the protein. This electroless plating is a catalytic chemical plating utilizing the catalytic activity of palladium and a metal is selectively precipitated on a metal surface having catalytic action. By applying immersion treatment to the treated membrane in an electroless plating liquid, the base metal is precipitated on palladium colloidal particles from said plating liquid and, therefore, protein can be detected with high sensitivity.

公开(公告)号：JPS63111465A

公开(公告)日：1988-05-16

申请号：JP25719086

申请日：1986-10-29

Applicant: AGENCY IND SCIENCE TECHN

Inventor： KOKUBU TOMOKUNI ,  IWAKURA MASAHIRO ,  OHASHI SHINICHI ,  TSUDA KEISHIRO

IPC: C12N15/02 ,  C12N15/00 ,  C12P21/00 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/535 ,  G01N33/577

Abstract: PURPOSE:To improve measurement sensitivity and reproducibility by utilizing the fused protein genetically bonded with labeling enzyme and antigen by a gene manipulation as a labeling antibody. CONSTITUTION:The coliform bacilli contg. the plasmid having the gene coding the dihydro folic acid reductase-leucine enkephalline fused protein (hereafter expressed as DHFR-L-Enk) are prepd. by the gene manipulation and are subjected to fractionating, refining and purifying after liquid culture to obtain the DHFR-L-Enk exhibiting a uniform band in SDS electrophoresis. Said liquid is added together with an antirabbit leucine enkephalline (hereafter expressed as L-Enk) antibody to cause reaction, by which a soln. for measuring enzyme activity is obtd. A DHFR substrate soln. is then added thereto to initiate reaction. A change in the absorption at 340nm wavelength is measured by a spectrophotometer, by which L-Enk is measured.

公开(公告)号：JPS6316679A

公开(公告)日：1988-01-23

申请号：JP12745586

申请日：1986-06-02

Applicant: AGENCY IND SCIENCE TECHN ,  KAWAMURA INST CHEM RES

Inventor： MINAMI SHINJI ,  TANABE GIICHI ,  TSUDA KEISHIRO ,  YONEHARA YOSHITOMO ,  KINOSHITA SHOICHI

IPC: H01L51/42 ,  C09K9/02 ,  H01L31/04

Abstract: PURPOSE:To obtain a photovoltaic element in which phthalocyanine having excellent stability is used as a dispersion medium by heat treating a phthalocyanine resin dispersion type photovoltaic element using a cadmium sulfide for a defect electrode to provide excellent photoelectric energy conversion efficiency. CONSTITUTION:A film made of polyvinylidene compound which contains phthalocyanine in a dispersive state is used as an optically active layer, and heat treated for an element interposed between the cadmium sulfide defect electrode and an ohmic electrode. An X-type nonmetal phthalocyanine resin dispersive film is formed on a cadmium sulfide layer (or the ohmic electrode) by heat treating, and formed on a photovoltaic element composed by forming the ohmic electrode (or the cadmium sulfide layer) or an element formed with an x-type nonmetal phthalocyanine resin dispersive film on the cadmium sulfide layer. In the latter case, after heat treating, the ohmic electrode is formed to construct a photovoltaic element.

公开(公告)号：JPS6269990A

公开(公告)日：1987-03-31

申请号：JP21081385

申请日：1985-09-24

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  TSUDA KEISHIRO

IPC: C12N15/09 ,  C12N1/20 ,  C12N15/70 ,  C12R1/19

Abstract: PURPOSE:To produce dihydrofolic acid reductase, by producing recombinant plamid PTA64-1 which is stably replicated with Escherichia coli and provides host Escherichia coli with trimethoprim resistance and ampicillin resistance. CONSTITUTION:A promoter sequence existing in a smaller one of two DNA fragments obtained by cleaving plasmid pTP52-1 with restriction enzyme PstI and C1aI is related to highly efficient manifestation of structural gene of dehydrofolic acid reductase. This DNA fragment is bonded to a larger one of two DNA fragments obtained by clevage of plasmid pTP63-21 with PstI and C1aI so that pTP64-1 can be replicated from the plasmid pTP63-21 and plasmid pTP52-1.

公开(公告)号：JPS61291994A

公开(公告)日：1986-12-22

申请号：JP13498685

申请日：1985-06-20

Applicant: AGENCY IND SCIENCE TECHN

Inventor： SORA TATSUO ,  SAKAI TSUKASA ,  TSUDA KEISHIRO

IPC: C25D13/00 ,  C25D13/04 ,  C25D13/08 ,  G01N27/30 ,  G01N27/327

Abstract: PURPOSE:To produce a uniform protein thin film free from the impurities on an electrode quickly and easily by dropping a small quantity of liquid contg. a charged colloidal particle on the electrode and bringing the other electrode into contact with the liquid from an upper part to perform the electrodeposition. CONSTITUTION:A movable arm 4 capable of regulating a position in the vertical direction is fitted to a vertical axis 2 fixed on a horizontal base 1 with a knob 3. A micrometer head 5 fitted with a metallic electrode 6 such as Pt used in case of the electrodeposition is fixed t the arm 4. On the other hand, a stage 8 of an electrode holder 9 mounted with a metallic electrode 10 is horizontally transferred on the above-mentioned horizontal base 1 by operating a knob 7 and the above-mentioned electrode 10 is properly positioned. Then after dropping a small quantity of a liquid 11 contg. a high molecular electrolyte such as protein and a charged colloidal particle such as a biomembrane fraction, the above-mentioned electrode 6 is descended by operating the knob 3 and brought into contact with the above-mentioned liquid 11. Thereafter both electrodes 6, 10 are joined to a DC electric power source 12 to perform the electrodeposition and a protein thin film is formed on the electrode 10.

公开(公告)号：JPS61196585A

公开(公告)日：1986-08-30

申请号：JP3728985

申请日：1985-02-26

Applicant: AGENCY IND SCIENCE TECHN ,  TOYO SODA MFG CO LTD

Inventor： MINAMI SHINJI ,  TANABE GIICHI ,  TSUDA KEISHIRO ,  YOKOYAMA TAIICHI

IPC: H01L31/04 ,  H01L51/30 ,  H01L51/42

Abstract: PURPOSE:To provide a photoelectric cell having a high conversion efficiency, by interposing a beta crystal phase quinacridon as an organic thin film material between two different electrodes and thereby forming a sandwitch structure. CONSTITUTION:A photoelectric cell is obtained in a manner that a beta crystal phase quinacridon evaporation film is formed by heat treating a quinacridon thin film which has been obtained by vacuum depositing on an electrode in an electric furnace or, alternatively, vacuum depositing quinacridon on a heated electrode, and another electrode is joined thereto. In the heat treating process of the quinacridon evaporation film, the heat treating temperature is set at 200-300 deg.C, preferably at 230-270 deg.C. The quinacridon evaporation film is thereby caused to make transition from the violet-red alpha crystal phase to the beta crystal phase. In the other process for obtaining the crystal phase quinacridon evaporation film, namely substrate heating process, quinacridon is vapor deposited on the heated substrate, whose temperature is set at 100-300 deg.C, and preferably at 130-180 deg.C.