公开(公告)号：NO20070946L

公开(公告)日：2007-04-11

申请号：NO20070946

申请日：2007-02-19

Applicant: HOFFMANN LA ROCHE

Inventor： DULAT MARA BOENITZ ,  KNAPPE WOLFGANG-REINHOLD ,  KRATZSCH PETER ,  LAGGERBAUER JESSICA ,  SCHMUCK RAINER

IPC: C12N9/04 ,  C12N15/10 ,  C12N15/53 ,  C12Q1/32 ,  G01N33/66

Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known fromto genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.

公开(公告)号：MX2007000560A

公开(公告)日：2007-03-07

申请号：MX2007000560

申请日：2005-07-19

Applicant: HOFFMANN LA ROCHE

Inventor： SCHMUCK RAINER ,  KNAPPE WOLFGANG-REINHOLD ,  BOENITZ-DULAT MARA ,  KRATZSCH PETER ,  LAGGERBAUER JESSICA

IPC: C12M1/00 ,  C12N9/00 ,  C12N15/00 ,  C12Q1/00 ,  G01N33/00

Abstract: La presente invencion se refiere a variantes mejoradas de glucosa-deshidrogenasas dependientes de pirroloquinolin-quinona (PQQ) solubles (s-GDH), que comprenden una insercion de aminoacidos entre las posiciones 428 y 429 como corresponde a la secuencia de aminoacidos conocida de Acinetobacter calcoaceticus, a genes que codifican tales variantes de s-GDH, a las proteinas de tales variantes de s-GDH con especificidad mejorada de sustrato para glucosa, y a diferentes aplicaciones de estas variantes de s-GDH, particularmente para determinar concentraciones de azucares, especialmente de glucosa en una muestra.

公开(公告)号：CA2570593A1

公开(公告)日：2006-01-26

申请号：CA2570593

申请日：2005-07-19

Applicant: HOFFMANN LA ROCHE

Inventor： KNAPPE WOLFGANG-REINHOLD ,  KRATZSCH PETER ,  SCHMUCK RAINER ,  LAGGERBAUER JESSICA ,  BOENITZ-DULAT MARA

IPC: C12N9/04 ,  C12M1/40 ,  C12N15/10 ,  C12N15/53 ,  C12Q1/32 ,  G01N33/66

Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known fromto genes encoding such variant s-GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.

公开(公告)号：CA2497245A1

公开(公告)日：2005-08-18

申请号：CA2497245

申请日：2005-02-14

Applicant: HOFFMANN LA ROCHE

Inventor： HORN CARINA ,  HOENES JOACHIM ,  MOSOIU DAN ,  KNAPPE WOLFGANG-REINHOLD ,  WITTMANN FRANZ

IPC: G01N33/48 ,  C12M1/34 ,  C12Q1/26 ,  C12Q1/54 ,  G01N33/52 ,  G01N21/00 ,  C12Q1/32 ,  G01N33/49 ,  G01N21/78

Abstract: The invention concerns test elements and methods for the optical detection o f an analyte in a sample.

公开(公告)号：HUE044628T2

公开(公告)日：2019-11-28

申请号：HUE09753073

申请日：2009-11-06

Applicant: HOFFMANN LA ROCHE

Inventor： KNAPPE WOLFGANG-REINHOLD ,  HILLER BERND ,  LEHR URSULA ,  ZIMMER VOLKER ,  PETRICH WOLFGANG ,  BEDON-GOMEZ LUIS

IPC: B01L3/00 ,  G01N21/84 ,  G01N33/52

公开(公告)号：BRPI0114962B1

公开(公告)日：2016-07-12

申请号：BR0114962

申请日：2001-10-20

Applicant: HOFFMANN LA ROCHE

Inventor： BUNK DANIELA ,  THYM DETLEF ,  KRATZSCH PETER ,  SCHMUCK RAINER ,  KNAPPE WOLFGANG-REINHOLD ,  SHAO ZHIXIN

IPC: C12N9/04 ,  G01N33/66 ,  C12M1/40 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N11/02 ,  C12N15/09 ,  C12N15/53 ,  C12P21/02 ,  C12Q1/32 ,  C12Q1/54

Abstract: "variações da glicose desidrogenase solúvel dependente de pirroloquinolina quinona". a presente invenção refere-se a variações melhoradas das glicose desidrogenases solúveis (s-gdh) dependentes de pirroloquinolina quinona (pqq), aos genes que codificam a s-gdh mutada, às proteínas mutantes de s-gdh com maior especificidade ao substrato pela glicose e às diferentes aplicações destas variações da s-gdh, particularmente para a determinação das concentrações de açúcar, especialmente da glicose em uma amostra.

公开(公告)号：CA2743068C

公开(公告)日：2015-12-29

申请号：CA2743068

申请日：2009-11-06

Applicant: HOFFMANN LA ROCHE

Inventor： KNAPPE WOLFGANG-REINHOLD ,  HILLER BERND ,  LEHR URSULA ,  ZIMMER VOLKER ,  PETRICH WOLFGANG ,  BEDON-GOMEZ LUIS DAVID

IPC: G01N33/52 ,  B01L3/00 ,  G01N21/86

Abstract: There is proposed a diagnostic test element (110) for detecting an analyte in a sample (126) of a body fluid, more particularly in whole blood. The diagnostic test element (110) comprises at least one test field (116) having at least one detection reagent, wherein the detection reagent is set up to pass through at least one detectable change in the presence of the analyte, more particularly an optical change. The test field (116) has at least one detection layer (118) which comprises the detection reagent and which comprises particles (137). At least 90% of all particles (137) of the detection layer (118) have an actual particle size of less than 10 micrometers.

公开(公告)号：CA2493918C

公开(公告)日：2012-08-28

申请号：CA2493918

申请日：2003-05-16

Applicant: HOFFMANN LA ROCHE

Inventor： HORN CARINA ,  HOENES JOACHIM ,  KNAPPE WOLFGANG-REINHOLD

IPC: C12M1/34 ,  C12Q1/26 ,  C08F2/00 ,  C12M1/40 ,  C12Q1/00 ,  C12Q1/32 ,  C12Q1/54 ,  G01N21/78 ,  G01N27/327 ,  G01N33/543

Abstract: The invention relates to a method and reagent system for detecting an analyte in a sample by means of an enzymatic reaction, involving the use of an enzyme-coenzyme complex as a stoichiometric reaction partner for the analyte present in the sample.

公开(公告)号：ES2275095T3

公开(公告)日：2007-06-01

申请号：ES03730061

申请日：2003-05-16

Applicant: HOFFMANN LA ROCHE

Inventor： HORN CARINA ,  HOENES JOACHIM ,  KNAPPE WOLFGANG-REINHOLD

IPC: C12M1/34 ,  C12Q1/00 ,  C08F2/00 ,  C12M1/40 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/54 ,  G01N21/78 ,  G01N27/327 ,  G01N33/543

Abstract: Método para preparar capas poliméricas sobre un soporte transparente, que comprende las siguientes etapas: (a) preparación de un soporte, (b) aplicación sobre el soporte de una composición líquida fotopolimerizable, (c) irradiación de la composición líquida fotopolimerizable a través del soporte, de tal modo que sobre el mismo se forme una capa polimérica de un grosor prefijado, y (d) eliminación del resto de composición líquida de la capa polimérica.

公开(公告)号：PL362548A1

公开(公告)日：2004-11-02

申请号：PL36254801

申请日：2001-10-20

Applicant: HOFFMANN LA ROCHE

Inventor： KRATZSCH PETER ,  SCHMUCK RAINER ,  BUNK DANIELA ,  SHAO ZHIXIN ,  THYM DETLEF ,  KNAPPE WOLFGANG-REINHOLD

IPC: G01N33/66 ,  C12M1/40 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/04 ,  C12N11/02 ,  C12N15/09 ,  C12N15/53 ,  C12P21/02 ,  C12Q1/32 ,  C12Q1/54

Abstract: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.