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    42.
    发明专利
    未知

    公开(公告)号：DE10253609A1

    公开(公告)日：2004-05-27

    申请号：DE10253609

    申请日：2002-11-15

    Applicant: LEICA MICROSYSTEMS

    Inventor： KNEBEL WERNER ,  STORZ RAFAEL

    IPC: G02B21/00

    Abstract: A scanning microscope, having a detector, arranged in a detection beam path, for receiving detection light proceeding from a sample, has between the sample and the detector an optical shutter means with which the detection beam path can be blocked. A control means for controlling the shutter means is provided. The detection light beam path can be blocked automatically outside the scanning operation and in the event of an excessive detection light power level.

    46.
    发明专利
    未知

    公开(公告)号：DE10357584A1

    公开(公告)日：2005-07-07

    申请号：DE10357584

    申请日：2003-12-08

    Applicant: LEICA MICROSYSTEMS

    Inventor： SEYFRIED VOLKER ,  STORZ RAFAEL

    IPC: G02B21/06 ,  G01J3/44 ,  G02B21/00 ,  G02B21/16

    Abstract: Separating different emission wavelengths in a scanning microscope, is new. Separating different emission wavelengths in a scanning microscope comprises: (a) scanning a specimen with an illuminating light beam by passing the illuminating light beam over the specimen using a beam deflector; (b) selectively applying each of excitation wavelengths (1, 2, 3) to the illuminating light beam during the scanning according to a pre-definable illumination scheme; (c) detecting emission light coming from the specimen using detector(s), where the detector is read out upon each selective applying of a respective excitation wavelength to provide respective corresponding detected signals; and (d) associating the detected signals with the respective excitation wavelength using the illumination scheme. The emission light includes emission wavelengths corresponding to the excitation wavelengths. An independent claim is also included for an apparatus for separating different emission wavelengths in a scanning microscope, comprising: (a) light source(s) configured to generate an illuminating light beam; (b) a beam deflector configured to scan a specimen with the illuminating light beam by passing the illuminating light beam over the specimen; (c) a light control device configured to selectively apply each of excitation wavelengths to the illuminating light beam during a scanning according to a pre-definable illumination scheme; (d) detector(s) configured to pick up emission light coming from the specimen; and (e) a processing device configured to associate the detected signals with the respective excitation wavelengths using the illumination scheme.

    48.
    发明专利
    未知

    公开(公告)号：DE10251345A1

    公开(公告)日：2004-05-19

    申请号：DE10251345

    申请日：2002-11-05

    Applicant: LEICA MICROSYSTEMS

    Inventor： HOPPE MARTIN ,  KNEBEL WERNER ,  MOELLMANN KYRA ,  STORZ RAFAEL

    IPC: G02B21/24 ,  A61B5/00 ,  A61B5/02

    Abstract: The invention concerns a method and an apparatus for investigating layers ( 1 ) of tissues in living animals using a microscope ( 2 ). The microscope ( 2 ) is focused onto a layer ( 1 ), and images of the layer ( 1 ) are acquired or optical measurements are performed on it. Positional changes of the layer ( 1 ) are brought about by movements of the animal or of its organs. The positional changes are sensed, and corresponding signals are generated. The signals are stored, together with the corresponding images or measurement results, for later evaluation; or they are processed in such a way that the positional changes are compensated for in order to investigate the layer ( 1 ). As a result, the layer ( 1 ) can be qualitatively or quantitatively investigated microscopically, irrespective of the movement of the animal or its organs.

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