公开(公告)号：US20030080442A1

公开(公告)日：2003-05-01

申请号：US10232997

申请日：2002-08-29

Applicant: Fluidigm Corp.

Inventor： Mark Unger

IPC: H01L023/28

CPC classification number: H01L21/00 ,  B01L3/0241 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  F04B43/043 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0051 ,  F16K99/0053 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008

Abstract: A valve structure comprises an elastomeric block formed with first and second microfabricated recesses separated by a membrane portion of the elastomeric block. The valve is actuated by positioning a compliant electrode on a first side of the first recess proximate to and in physical communication with the membrane. Where the valve is to be electrostatically actuated, a second electrode is positioned on a second side of the first recess opposite the first side. Application of a potential difference across the electrodes causes the compliant electrode and the membrane to be attracted into the flow channel. Where the valve is to be electrostrictively actuated, a second electrode is positioned on the same side of the recess as the compliant electrode. Application of a potential difference across the electrodes causes the electrodes to be attracted such that elastomer membrane portion material between them is compressed and bows into the flow channel. Either of the electrostrictively or the electrostatically-actuated valve structures may include an electrically-conducting fluid in the second recess to serve as the compliant electrode.

Abstract translation: 阀结构包括由弹性体块的膜部分分开的第一和第二微细加工凹部形成的弹性体块。 通过将顺应性电极定位在第一凹部的靠近膜并且与膜物理连通的第一侧上来致动阀。 在阀被静电致动的地方，第二电极位于与第一侧相对的第一凹槽的第二侧上。 在电极之间施加电位差使得柔性电极和膜被吸引到流动通道中。 在阀被电致伸缩的情况下，第二电极位于与顺应性电极相同的凹槽侧。 在电极之间施加电位差导致电极被吸引，使得它们之间的弹性体膜部分材料被压缩并弯曲到流动通道中。 电致伸缩阀或静电致动阀结构中的任何一个可以包括在第二凹槽中用作柔性电极的导电流体。

公开(公告)号：US20040224380A1

公开(公告)日：2004-11-11

申请号：US10734963

申请日：2003-12-11

Applicant: Fluidigm Corp.

Inventor： Hou-Pu Chou ,  Antoine Daridon ,  Kevin Farrell ,  Brian Fowler ,  Yish-Hann Liau ,  Ian D. Manger ,  Hany Ramez Nassef ,  William Throndset

IPC: G01N033/00 ,  C12M001/34 ,  C12Q001/02

CPC classification number: G01N35/00 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N15/1456 ,  G01N15/1468 ,  G01N15/1484 ,  G01N2015/0288 ,  G01N2015/1075 ,  G01N2015/149 ,  G01N2015/1493 ,  Y10T436/11 ,  Y10T436/2575

Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

Abstract translation: 本发明提供了用于微流体操纵和/或检测诸如细胞和/或珠粒的装置，方法和试剂盒的系统。 本发明提供了用于微粒操作和/或分析颗粒例如细胞，病毒，细胞器，珠粒和/或囊泡的装置，方法和试剂盒的系统。 本发明还提供用于进行这些操作和分析的微流体机理。 这些机制可以实现颗粒的控制输入，运动/定位，保留/定位，处理，测量，释放和/或输出。 此外，这些机制可以以任何合适的顺序组合和/或在系统内任何适当次数使用。 因此，这些组合可以允许将粒子分类，培养，混合，处理和/或测定为单粒子，粒子的混合组，粒子阵列，非均匀粒子组和/或均匀粒子组，其中 其他，串联和/或并行。 此外，这些组合可以使微流体系统能够重复使用。 此外，这些组合可以允许在比以前可能的更短的时间尺度上测量颗粒对治疗的反应。 因此，本发明的系统可以允许广泛范围的细胞和颗粒测定，例如药物筛选，细胞特征化，研究研究和/或临床分析等，以缩小到微流体大小。 这种按比例缩小的测定可能使用较少的样品和试剂，可能较少的劳动密集型和/或可能比可比较的大流控测定更具信息性。

公开(公告)号：US20030138829A1

公开(公告)日：2003-07-24

申请号：US10306798

申请日：2002-11-27

Applicant: Fluidigm Corp.

Inventor： Marc Unger ,  Ian D. Manger ,  Michael Lucero ,  Yong Yi ,  Emily Miyashita-Lin ,  Anja Weinecke ,  Geoffrey Facer

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  C12M001/34

CPC classification number: C12M1/38 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B60/14 ,  F16K11/20 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T137/2076 ,  Y10T137/2496 ,  Y10T137/86292 ,  Y10T137/87249 ,  Y10T436/2575 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

公开(公告)号：CA2874343C

公开(公告)日：2021-11-09

申请号：CA2874343

申请日：2013-05-21

Applicant: FLUIDIGM CORP

Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B30/00 ,  C40B70/00 ,  G01N1/28 ,  G01N1/38 ,  G01N33/48

Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out "in parallel," i.e., essentially simultaneously in the separate reaction volumes.

公开(公告)号：SG11202001088PA

公开(公告)日：2020-03-30

申请号：SG11202001088P

申请日：2018-08-20

Applicant: FLUIDIGM CORP

Inventor： QIN JIAN ,  CHEN PEILIN ,  RAMAKRISHNAN RAMESH

IPC: C12N15/10 ,  C12Q1/6806

Abstract: This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.

公开(公告)号：SG10201908167YA

公开(公告)日：2019-10-30

申请号：SG10201908167Y

申请日：2014-09-04

Applicant: FLUIDIGM CORP

Inventor： EGIDIO CAMILA ,  RAMAKRISHNAN RAMESH ,  RUFF DAVID

Abstract: PROXIMITY ASSAYS FOR DETECTING NUCLEIC ACIDS AND PROTEINS IN A SINGLE CELL Methods and reagents for detection and analysis of nucleic acids and proteins using proximity extension assays. [Figure 1]

公开(公告)号：CA3072463A1

公开(公告)日：2019-02-21

申请号：CA3072463

申请日：2018-08-20

Applicant: FLUIDIGM CORP

Inventor： QIN JIAN ,  CHEN PEILIN ,  RAMAKRISHNAN RAMESH

IPC: C12N15/10 ,  C12Q1/6806

Abstract: Disclosed herein is a method and kit thereof for isolating nucleic acids from wax-embedded samples using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids using ethanol, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.

公开(公告)号：CA2757560C

公开(公告)日：2018-11-13

申请号：CA2757560

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

IPC: C12Q1/68 ,  B81B7/04 ,  C12M1/34 ,  C12M1/36 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  C40B20/04 ,  C40B30/04 ,  C40B70/00 ,  G01N33/50

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：CA3027423A1

公开(公告)日：2018-01-18

申请号：CA3027423

申请日：2017-07-12

Applicant: FLUIDIGM CORP

Inventor： LIVAK KENNETH J ,  FEKETE RICHARD A

IPC: C12Q1/68

Abstract: Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.

公开(公告)号：SG10201707485YA

公开(公告)日：2017-10-30

申请号：SG10201707485Y

申请日：2014-03-14

Applicant: FLUIDIGM CORP

Inventor： WEST JASON A A ,  FOWLER BRIAN

Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment. A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.