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公开(公告)号：KR1020090028212A
公开(公告)日：2009-03-18
申请号：KR1020070093643
申请日：2007-09-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K35/648
CPC classification number: A61K35/648
Abstract: A manufacturing method of Scolopendra subspinipes extract making immunogenicity of animal is provided to be useful as immunostimulator preventing and treating efficiently disease of the animal, an adjuvant improving effect of vaccine and an adjuvant treatment making therapeutic efficacy reinforced. A manufacturing method of Scolopendra subspinipes extract making immunogenicity of animal comprises steps of: put the Scolopendra subspinipes powder into distilled water; obtaining extract by heating obtained solution, filtering obtained extract using filter paper firstly, filtering filtered material using filter paper secondly and sterilizing filtered material.
Abstract translation: 提供了提供动物免疫原性的Scolopendra subspinipes提取物的制造方法，可用作有效预防和治疗动物疾病的免疫刺激剂，疫苗的辅助改善效果和辅助治疗，增强治疗功效。 Scolopendra subspinipes提取物制备动物免疫原性的方法包括以下步骤：将Scolopendra subspinipes粉末放入蒸馏水中; 通过加热获得的溶液获得提取物，首先使用滤纸过滤获得的提取物，然后用滤纸过滤过滤材料并对过滤材料进行灭菌。
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公开(公告)号：KR1020090013600A
公开(公告)日：2009-02-05
申请号：KR1020070077855
申请日：2007-08-02
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: An immunostimulant containing the lystic extract of Candida sake is provided to increase activity of neutrophil and lymphocyte as major immune cells of animals, thereby protecting animals from diseases. The immunostimulant contains the lystic extract of Candida sake which is prepared by cultivating Candida sake in yeast malt extract agar broth at 30 deg. C for 72 hours, centrifuging the cultured fluid at 3000Xg for 30 minutes and collecting it, rinsing the collected pellet with phosphate buffered saline(PBS) of pH 7.2 and suspending the rinsed pellet in PBS, adding 5% of TNT solution containing 50mM of Tris-HCl, 0.15M of NaCl and 4% Triton x-114 as solubilizing agent into the Candida sake suspension, stirring the mixture at room temperature for 2-3 days to solubilize the Streptomyces albus, and regulating pH of the solution to 6.5-7.2, and drying the solution in the thermostatic chamber.
Abstract translation: 提供含有念珠菌精液提取物的免疫刺激剂，以增加嗜中性粒细胞和淋巴细胞作为动物主要免疫细胞的活性，从而保护动物免受疾病。 免疫刺激剂含有通过在30℃下在酵母麦芽提取物琼脂培养液中培育念珠菌精油而制备的念珠菌清酒。 培养72小时，将培养液以3000Xg离心30分钟并收集，用pH7.2的磷酸盐缓冲盐水（PBS）冲洗收集的沉淀物，并将漂洗的沉淀悬浮于PBS中，加入5％含有50mM Tris -HCl，0.15M NaCl和4％Triton x-114作为增溶剂加入到念珠菌悬浮液中，在室温下搅拌混合物2-3天以溶解链霉菌，并将溶液的pH调节至6.5-7.2 ，并干燥恒温室中的溶液。
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公开(公告)号：KR1020090012575A
公开(公告)日：2009-02-04
申请号：KR1020070076507
申请日：2007-07-30
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K31/70 , A61K36/48 , A61K36/185 , A61P37/02
CPC classification number: A61K38/168 , Y10S514/885
Abstract: Glycine max (L.) Merrill lectin is provided to reinforce immunogenicity of animals, to improve effect of an immunoadjuvant and a vaccine preventing and treating disease of the animals efficiently and to increase defence ability aspecific to attack inoculation of pathogenic bacteria. An immunoadjuvant comprises Glycine max (L.) Merrill lectin as an active ingredient. Additionally, the Glycine max (L.) Merrill lectin is used for a vaccine throne and an adjuvant treatment as the active ingredient.
Abstract translation: 提供大豆（L.）美林凝集素以增强动物的免疫原性，提高免疫佐剂的效果和预防和治疗动物疾病的疫苗，并提高对病原菌接种的防御能力。 免疫佐剂包含甘氨酸（L.）美林凝集素作为活性成分。 另外，甘氨酸（L.）美林凝集素用作疫苗王位和辅助治疗作为活性成分。
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公开(公告)号：KR1020080095988A
公开(公告)日：2008-10-30
申请号：KR1020070040731
申请日：2007-04-26
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A feed additive using a coptic rhizome and forsythia fruit extract is provided to accelerate the growth of animal, to reinforce an immune function and to prevent diseases, by adding predetermined amount of extracts obtained from Forsythiae Fructus and Coptidis Rhizoma and supplying it to domestic animals, thereby substituting antibiotics. A feed additive using a coptic rhizome and forsythia fruit extract comprises Coptis japonica extract 50 weight%, forsythia extract 10 weight%, and dextrose 30 weight% and Glycyrrhiza extract 10 weight%.
Abstract translation: 提供使用科普特根和连翘果提取物的饲料添加剂，通过加入预定量的连翘和黄连提取物提取动物，增加免疫功能，增强免疫功能，预防疾病，并将其提供给家畜， 从而代替抗生素。 使用科普特根和连翘果提取物的饲料添加剂包括50重量％的黄连提取物，10重量％的连翘提取物和30重量％的葡萄糖和10重量％的甘草提取物。
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Patent Agency Ranking
公开(公告)号：KR1020080032809A
公开(公告)日：2008-04-16
申请号：KR1020060098780
申请日：2006-10-11
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: Y02A50/473 , A61K35/74 , A61K36/06 , C12N1/16 , C12N1/20
Abstract: A method for preparing a cell lysis composition of bacterial and yeast cell wall is provided to improve yield of cell wall, and selectively employ antigen substances and immune-enhancing substances in the cell wall by using biochemical and chemical treatment, so that the composition is useful as vaccine antigen, heterologous delivery system, immune enhancer, vaccine assistant and complex feed additive. A method for preparing a cell lysis composition of bacterial and yeast cell wall comprises the steps of: culturing gram positive bacteria, gram negative bacteria and yeast; treating the cultured gram positive bacteria, gram negative bacteria and yeast biochemically and chemically by using transparent outer membrane extraction, TNT(Tris-HCl, sodium chloride, Triton X-114) method, NaOH-EDTA(sodium hydroxide-(ethylene dinitrilo) tetraacetic acid), TMC(Tris-HCl, magnesium chloride, calcium chloride) method or TET(Tris-HCl, EDTA, Triton X-100) method; and extracting useful ingredients of cell wall from the treated gram positive bacteria, gram negative bacteria and yeast, wherein the gram negative bacteria include Escherichia coli 055 and Bordetella pertussis; the gram positive bacteria include Staphylococcus aureus Cowan 1 and Erysipelothrix rhusiopathiae T2; and the yeast is Saccharomyces cerevisiae or Candida albicans. Further, the useful ingredients of cell wall are selected from a group consisting of a transparent cell outer membrane, a cell wall and bacteriolysant.
Abstract translation: 提供细菌和酵母细胞壁细胞裂解组合物的制备方法，以提高细胞壁的产量，并通过生物化学和化学处理选择性地在细胞壁中使用抗原物质和免疫增强物质，使组合物有用 作为疫苗抗原，异源递送系统，免疫增强剂，疫苗助剂和复合饲料添加剂。 制备细菌和酵母细胞壁的细胞裂解组合物的方法包括以下步骤：培养革兰氏阳性菌，革兰氏阴性细菌和酵母; 通过使用透明的外膜提取，TNT（Tris-HCl，氯化钠，Triton X-114）方法，NaOH-EDTA（氢氧化钠 - （亚乙基二硝基）四乙酸）处理培养的革兰氏阳性细菌，革兰氏阴性细菌和酵母生物化学和化学 酸），TMC（Tris-HCl，氯化镁，氯化钙）法或TET（Tris-HCl，EDTA，Triton X-100）法; 并从经处理的革兰氏阳性细菌，革兰氏阴性细菌和酵母提取细胞壁的有用成分，其中革兰氏阴性细菌包括大肠杆菌055和百日咳博德特氏菌; 革兰氏阳性细菌包括金黄色葡萄球菌Cowan 1和Erysipelothrix rhusiopathiae T2; 酵母是酿酒酵母或白色念珠菌。 此外，细胞壁的有用成分选自透明细胞外膜，细胞壁和细菌悬液。
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66.发明授权동물혈청을 첨가하지 않은 돈단독균 배지 및 그 배지에서 배양한 돈단독균으로 제조되는 생균 및 사균백신 有权不含动物血清和猪丹毒的白纹丝杆菌血管病毒培养基用在该培养基上培养的细菌制备的活的和灭活的疫苗
公开(公告)号：KR100769356B1
公开(公告)日：2007-11-01
申请号：KR1020060007212
申请日：2006-01-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명자는 돈단독균의 발육능을 향상시키기 위하여 개발한 배지에서의 돈단독균 발육성과 이 배지에서 배양한 돈단독균으로 만든 불활화백신의 마우스에서의 면역원성을 시험하여 다음과 같은 결과를 얻었다.
돈단독 생균수는 기존의 브레인 하트 인퓨전(Brain Heart Infusion, BHI) 배지에 5-10% 소혈청을 첨가하여 배양하는 것보다 약 10배 이상의 균수 향상을 보였다.
37℃에서 18-20시간 배양후 배양배지의 pH가 7.0으로 매우 안정적인 pH를 나타내었다.
상기의 개발 배지에서 배양한 돈단독균으로 제조한 돈단독불활화 백신의 마우스에서의 항체형성능은 양호하였다.
돈단독 배양배지, 동물혈청 무첨가-
公开(公告)号：KR100593254B1
公开(公告)日：2006-06-28
申请号：KR1020040033170
申请日：2004-05-11
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N1/20
Abstract: 본 발명은 엔테로코커스 에스피 58 (KACC 91099) 및 이의 용도에 관한 것으로서, 상기 균주는 내산성, 내담즙성 및 생체 안전성이 인정되고, 병원성 세균 억제활성을 가지며, 장내에서 독립된 균총(Niche)으로서의 활성과 이에 근거한 길항 작용을 통한 병원성 균총의 증식을 감소시켜 질병을 예방하는 효과가 있다.
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公开(公告)号：KR100293571B1
公开(公告)日：2001-09-17
申请号：KR1019980024503
申请日：1998-06-27
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/68
Abstract: 본 발명은 가축에서 설사를 일으키는 병원성 대장균이 지닌 장독소 생성유전자들을 신속하게 확인할 수 있는 멀티플렉스 PCR 기법(multiplex polymerase chain reaction-based method)에 관한 것으로, 더욱 상세하게는 공지의 이열성독소(LT : heat-labile enterotoxin) 생성유전자에 대한 PCR 프라이머(primer)와 함께 한번의 PCR 반응에 동시에 적용할 수 있는 내열성독소(ST : heat-stable enterotoxin) 생성유전자에 대한 PCR 프라이머를 개발하고, 이들 프라이머들을 동시에 이용하는 대장균 장독소 유전자의 동시 검출을 위한 멀티플렉서 PCR 기법에 관한 것이다.
본 발명에 의해 제공되는 내열성독소(STIa) 생성유전자에 대한 PCR 프라이머는, 공지의 LT 생성유전자에 대한 PCR 프라이머와 함께 한번의 PCR 반응에 동시에 적용할 수 있어, 1회의 PCR 수행으로 대장균의 병원성 인자인 2종의 장독소 생성유전자를 동시에 검출할 수 있으며, 각종 생화학적 시험과 혈청학적 시험을 거치지 않고서도 대장균 설사증을 신속하고 정확하게 진단할 수 있다.-
公开(公告)号：KR1020010037612A
公开(公告)日：2001-05-15
申请号：KR1019990045242
申请日：1999-10-19
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A23K1/18
Abstract: PURPOSE: A feed additive is provided to prevent and treat the pig colon bacillus diarrhea by using dried yolk powder having an idioantibody. CONSTITUTION: A testing vaccine manufactured by using a cilium antigen of the colon bacillus is inoculated on a layer and immunized. The eggs are disinfected with alcohol and the egg yolk is aseptically collected in an Erlenmeyer Flask. The collected yolk is injected into a powder dryer to gather yolk powder. The injecting temperature of the powder dryer is 140deg.C and the releasing temperature is 72deg.C. The dried powder weight per 1ml vol and the moisture content of the egg yolk are 0.38g and 2%, respectively. The value of an antibody is rarely changed before and after the drying.
Abstract translation: 目的：提供饲料添加剂，通过使用具有不良反应的干燥的蛋黄粉来预防和治疗猪大肠杆菌腹泻。 构成：将通过使用大肠杆菌的纤毛抗原制造的测试疫苗接种在一层上并免疫。 鸡蛋用酒精消毒，蛋黄被无菌地收集在锥形瓶中。 将收集的蛋黄注入粉末干燥器中以收集蛋黄粉末。 粉末干燥机的注射温度为140℃，脱模温度为72℃。 每1ml体积的干燥粉末重量和蛋黄的水分含量分别为0.38g和2％。 干燥前后抗体的值很少变化。
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