公开(公告)号：KR1020150003038A

公开(公告)日：2015-01-08

申请号：KR1020130075940

申请日：2013-06-28

Applicant: 삼성전자주식회사

Inventor： 박은영 ,  김진우 ,  송여주 ,  이영민 ,  이우용 ,  이홍순 ,  조화영 ,  박재찬 ,  박진환 ,  조광명

IPC: C12N1/21 ,  C12N15/53 ,  C12N15/77 ,  C12P7/18

Abstract: 숙시닉 세미알데히드 데히드로게나제 유전자가 파괴된 미생물 및 이를 이용한 대사물질의 생산 방법을 제공한다. 상기 미생물을 이용하여 유용한 대사물질을 고농도로 생산하는 것이 가능하다.

Abstract translation: 本发明提供：破坏琥珀酸半醛脱氢酶基因的微生物; 以及使用其代谢产物的方法。 根据本发明，可以通过使用微生物来生产高密度的有用的代谢物。 本发明的实施方案提供了编码琥珀酸半醛脱氢酶（SSADH）的基因被灭活或还原的微生物。 微生物属于棒杆菌属 琥珀酸半醛脱氢酶是包含SEQ ID：1，SEQ ID：2或SEQ ID：3的氨基酸序列的多肽。 根据本发明的一个实施方案，其中琥珀酸半醛脱氢酶基因失活或减少的微生物可用于生产有用的代谢物。

公开(公告)号：KR1020140041304A

公开(公告)日：2014-04-04

申请号：KR1020120116852

申请日：2012-10-19

Applicant: 삼성전자주식회사

Inventor： 박선민 ,  김현령 ,  박재찬 ,  김민상 ,  채수영

IPC: A61K9/127 ,  A61K47/48

CPC classification number: A61K47/42 ,  A61K9/127 ,  A61K31/704 ,  A61K47/28

Abstract: The present invention relates to a liposome comprising an elastin-like polypeptide (ELP) conjugated to a moiety containing a hydrophobic group, a chemosensitizer, and an anticancer agent, wherein the ELP conjugated to moiety containing the hydrophobic group is filled in a lipid bilayer. The present invention provides a pharmaceutical composition including the liposome and a method for delivering the chemosensitizer and the anticancer agent to a target site of a subject by using the liposome. [Reference numerals] (AA) Cell viability(%); (BB) Free doxorubicin; (CC) Glass doxorubicin

Abstract translation: 本发明涉及包含与含有疏水基团的部分缀合的弹性蛋白样多肽（ELP），化学敏化剂和抗癌剂的脂质体，其中与含有疏水基团的部分缀合的ELP填充在脂质双层中。 本发明提供了包含脂质体的药物组合物和通过使用脂质体将化学敏化剂和抗癌剂递送到受试者的靶位点的方法。 （AA）细胞活力（％）; （BB）免费多柔比星; （CC）玻璃多柔比星

公开(公告)号：KR1020140015086A

公开(公告)日：2014-02-06

申请号：KR1020120082815

申请日：2012-07-27

Applicant: 삼성전자주식회사

Inventor： 이규상 ,  이영민 ,  이우용 ,  구현민 ,  김진우 ,  박영경 ,  박진환 ,  조화영 ,  박재찬

IPC: C12N1/21 ,  C12N15/52 ,  C12N15/74 ,  C12P7/16

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N15/52 ,  C12N15/77 ,  C12Y101/01037 ,  C12Y101/05004 ,  Y02P20/52

Abstract: One embodiment of the present invention relates to a metabolism network model of Corynebacterium glutamicum for producing 1,4-BDO by Corynebacterium glutamicum, which is a strain that does not produce 1,4-BDO in a natural state. On the basis of the network model, metabolic properties of Corynebacterium glutamicum are analyzed. By predicting effects of a deletion target enzyme (ldhA, mqo or mdh) in a state in which Cat1, sucD, 4hbD, cat2 and adhE are included, and removing them, production efficiency of 1,4-BDO and increase of 1,4-BDO under the same fermentation conditions are confirmed. In addition, transformation microorganism produced by the method can produce 1,4-BDO in high efficiency, and therefore, can be effectively used in the industries.

Abstract translation: 本发明的一个实施方案涉及谷氨酸棒杆菌的代谢网络模型，用于通过谷氨酸棒杆菌生产1,4-BDO，其是在天然状态下不产生1,4-BDO的菌株。 在网络模型的基础上，分析谷氨酸棒杆菌的代谢特性。 通过预测在包含Cat1，sucD，4hbD，cat2和adhE的状态下缺失目标酶（ldhA，mqo或mdh）的作用并除去它们，1,4-BDO的生产效率和增加1,4 -BDO在相同的发酵条件下得到确认。 另外，通过该方法生产的转化微生物可以高效率地生产1,4-BDO，因此可以有效地用于工业中。

公开(公告)号：KR1020130042905A

公开(公告)日：2013-04-29

申请号：KR1020110107055

申请日：2011-10-19

Applicant: 삼성전자주식회사

Inventor： 김민상 ,  김현령 ,  박재찬 ,  채수영 ,  박선민

IPC: A61K9/127 ,  A61K31/56 ,  A61K47/48 ,  A61K38/08

CPC classification number: A61K41/0052 ,  A61K41/0028 ,  A61K47/62 ,  A61K47/6911 ,  A61K9/127 ,  A61K31/56 ,  A61K38/08 ,  A61K47/50

Abstract: PURPOSE: A liposome containing an elastine-like polypeptide(ELP) and a use thereof are provided to reduce the amount of drug release, and to maintain stable state. CONSTITUTION: A liposome contains: a lipid bilayer; an elastine-like polypeptide(ELP) in which hydrophobic group-containing moiety is conjugated; and a lipid bilayer stabilizing agent. The moiety is filled in the lipid bilayer. The lipid bilayer stabilizing agent is steroid which is incorporated in the lipid bilayer. The lipid bilayer stabilizing agent is selected from the group consisting of sterol or a derivative thereof, spingolipid or a derivative thereof, and a combination thereof. The lipid bilayer stabilizing agent is selected from the group consisting of cholesterol, beta-cholesterol, sistosterol, ergosterol, stigmasterol, 4,22 stigmastadiene-3-one, stigmasterol acetate, lanosterol, and a combination thereof. [Reference numerals] (AA) Calcein release(%); (BB) Cholesterol molar ratio: 25.8; (CC) Cholesterol molar ratio: 41; (DD) Temperature(°C);

Abstract translation: 目的：提供含有弹性蛋白样多肽（ELP）的脂质体及其用途，以减少药物释放量并保持稳定状态。 构成：脂质体含有：脂质双层; 含有疏水基团的部分共轭的弹性蛋白样多肽（ELP） 和脂质双层稳定剂。 该部分填充在脂质双层中。 脂质双层稳定剂是掺入脂质双层的类固醇。 脂质双层稳定剂选自固醇或其衍生物，鞘脂或其衍生物及其组合。 脂质双层稳定剂选自胆固醇，β-胆固醇，西沙利醇，麦角甾醇，豆甾醇，豆甾醇，4,2-二十二烷-3-酮，醋酸豆蔻醇，羊毛甾醇及其组合。 （AA）钙黄绿素释放（％）; （BB）胆固醇摩尔比：25.8; （CC）胆固醇摩尔比：41; （DD）温度（℃）;

公开(公告)号：KR1020130007687A

公开(公告)日：2013-01-21

申请号：KR1020110062316

申请日：2011-06-27

Applicant: 삼성전자주식회사 ,  서울대학교산학협력단

Inventor： 김재영 ,  박재찬 ,  박성민 ,  구현민 ,  박영경 ,  서진호 ,  박용철

IPC: C12N1/19 ,  C12N1/21 ,  C12N9/02 ,  C12P7/06

CPC classification number: C12N9/0006 ,  C12N9/1205 ,  C12Y101/01009 ,  C12Y101/01021 ,  C12Y207/01017 ,  Y02E50/17

Abstract: PURPOSE: A modified microorganism with improved xylose utilization is provided to produce chemical materials. CONSTITUTION: A modified microorganism converts xylose into xylitol by xylose reductase, xylitol into xylulose by xylitol dehydrogenase, and xylulose into xylulose-5-phosphate by xylulokinase. The microorganism produces a chemical material using xylose. Xylose is converted into xylitol by xylose reductase. The xylose reductase and xylitol dehydrogenase activities are derived from Pichia stipitis. The xylulokinase activity is derived from Saccharomyces cerevisiae. The modified microorganism is E.coli or Kluyveromyces marxianus. The chemical material includes alcohol, organic acid, amino acid, or vitamin. The modified microorganism is deposited by deposit number KCTC11951BP, KCTC11952BP or KCTC11953BP.

Abstract translation: 目的：提供木糖利用率提高的修饰微生物，以生产化学物质。 构成：修饰的微生物通过木糖还原酶将木糖转化为木糖醇，通过木糖醇脱氢酶将木糖醇转化成木酮糖，并通过木酮糖激酶将木酮糖转化成木酮糖-5-磷酸。 微生物使用木糖产生化学物质。 木糖由木糖还原酶转化成木糖醇。 木糖还原酶和木糖醇脱氢酶活性源自树干毕赤酵母。 木酮糖激酶活性来源于酿酒酵母（Saccharomyces cerevisiae）。 修饰的微生物是大肠杆菌或马克斯克鲁维酵母（Kluyveromyces marxianus）。 化学物质包括酒精，有机酸，氨基酸或维生素。 修饰的微生物通过保藏号KCTC11951BP，KCTC11952BP或KCTC11953BP沉积。

公开(公告)号：KR1020130001509A

公开(公告)日：2013-01-04

申请号：KR1020110062312

申请日：2011-06-27

Applicant: 삼성전자주식회사

Inventor： 김재영 ,  박성민 ,  박영경 ,  유병조 ,  박재찬 ,  조화영 ,  구현민

IPC: C12N15/53 ,  C12N15/63 ,  C12P7/52

CPC classification number: C12P7/42 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y203/01008

Abstract: PURPOSE: A recombinant microorganism which produces 3-hydroxypropionic acid(3-HP) using a malonic semialdehyde reduction pathway is provided to remarkably enhance 3-HP productivity by gene manipulation. CONSTITUTION: A recombinant microorganism for producing 3-hydroxypropionic acid has a pathway for sequentially producing pyruvate, acetyl Co, malonyl-CoA, and malonic semialdehyde. The microorganism includes Escherichia sp., Saccharomyces sp., or Kluyveromyces sp. A gene encoding lactate dehydrogenase is IdhA or a homolog or a variant thereof. A gene encoding phosphotransacetylase is pta or a homolog or a variant thereof.

Abstract translation: 目的：提供使用丙二醛还原途径产生3-羟基丙酸（3-HP）的重组微生物，通过基因操作显着提高3-HP生产力。 构成：用于产生3-羟基丙酸的重组微生物具有顺序产生丙酮酸，乙酰辅酶A，丙二酰辅酶A和丙二醛半醛的途径。 微生物包括大肠杆菌，酵母属或克鲁维酵母属。 编码乳酸脱氢酶的基因是IdhA或其同源物或其变体。 编码磷酸转乙酰酶的基因是pta或其同系物或其变体。

公开(公告)号：KR1020130001121A

公开(公告)日：2013-01-03

申请号：KR1020120061819

申请日：2012-06-08

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 정순천 ,  구현민 ,  김재영 ,  김지은 ,  김진우 ,  박영경 ,  이소영 ,  조화영 ,  권대혁 ,  박재찬

IPC: C12N1/19 ,  C12N1/21 ,  C12N15/53 ,  C12P7/56

Abstract: PURPOSE: A modified microorganism for producing lactic acids and a method for producing lactic acids are provided to produce lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids with high efficiency has LDH activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, and Rattus norvegicus. The modified microorganism is yeast or bacteria. The modified microorganism is E.coli or Kluyveromyces marxianus. The modified microorganism produces lactic acids with 12.2% or more of glucose. An expression vector for producing the modified microorganism comprises: a replication origin; a promoter; a polynucleotide; and a terminator. A method for producing lactic acids comprises: a step of culturing the modified microorganism in a medium containing glucose; and a step of collecting lactic acids from the culture.

Abstract translation: 目的：提供用于生产乳酸的改性微生物和生产乳酸的方法，以在酸性条件下高效生产乳酸。 构成：用于高效生产乳酸的改良微生物具有日本芍药，鸟腥草，Tur ps属，and and属的LDH活性。 修饰的微生物是酵母或细菌。 修饰的微生物是大肠杆菌或马克斯克鲁维酵母（Kluyveromyces marxianus）。 经修饰的微生物产生具有12.2％或更多葡萄糖的乳酸。 用于产生修饰微生物的表达载体包括：复制起点; 启动子 多核苷酸; 和终结者。 制备乳酸的方法包括：在含有葡萄糖的培养基中培养经修饰的微生物的步骤; 以及从培养物中收集乳酸的步骤。

公开(公告)号：KR1020130001103A

公开(公告)日：2013-01-03

申请号：KR1020110139520

申请日：2011-12-21

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 정순천 ,  구현민 ,  김재영 ,  김지은 ,  김진우 ,  박영경 ,  이소영 ,  조화영 ,  권대혁 ,  박재찬

IPC: C12N15/63 ,  C12N1/21 ,  C12N15/53 ,  C12P7/56

Abstract: PURPOSE: A modified microorganism for producing lactic acids is provided to prepare lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids has a lactate dehydrogenase(LDH) activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus. The modified microorganism is Escherichia sp. or Kluyveromyces sp. The modified microorganism produces lactic acids with 34% or more of glucose. An expression vector contains: a replication origin for constructing the modified microroganisms; a promoter; a polynucleotide coding LDH activation of one or more species selected from the group consisting of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus; and a terminator. The replication origin is ARS/CEN replication origin.

Abstract translation: 目的：提供一种用于生产乳酸的改性微生物，用于在酸性条件下高效制备乳酸。 构成：用于生产乳酸的改良微生物具有日本芍药（Ornisorhynchus anatinus，Tursiops truncates）或褐藻（Rattus norvegicus）的乳酸脱氢酶（LDH）活性。 修饰的微生物是大肠杆菌属 或克鲁维酵母属 经修饰的微生物产生具有34％以上葡萄糖的乳酸。 表达载体包含：用于构建修饰的微结构的复制起点; 启动子 编码一种或多种选自以下的物质的LDH活化的多核苷酸：日本Pe us，鸟，us us us，，，;;;;;;;;;;;;;;;;;; 和终结者。 复制起点是ARS / CEN复制来源。

公开(公告)号：KR1020130000883A

公开(公告)日：2013-01-03

申请号：KR1020110061677

申请日：2011-06-24

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 구현민 ,  박성민 ,  유병조 ,  이기성 ,  박재찬 ,  권대혁

IPC: C12N15/70 ,  C12N15/81 ,  C12N15/67

CPC classification number: C12N15/815 ,  C12P21/00 ,  C12P21/02

Abstract: PURPOSE: An expression vector which overexpresses target proteins, and a method for producing the target protein are provided to obtain the proteins in K. marxianus. CONSTITUTION: An expression vector contains: a replication origin; a CYC promoter, a TEF promoter, a GPD promoter, or an ADH promoter; and a terminator. The CYC promoter contains a sequence of sequence number 1 or a sequence having 70% or more sequence homology with the sequence of sequence number 1. The TEF promoter has a sequence of sequence number 2 or a sequence with 70% or more sequence homology with the sequence of sequence number 2. GPD promoter contains a sequence of sequence number 3 or a sequence having 70% or more homology with the sequence of sequence number 3. The ADH promoter has a sequence of sequence number 4 or a sequence having 70% or more sequence homology with the sequence of sequence number 4.

Abstract translation: 目的：提供过表达靶蛋白的表达载体和产生靶蛋白的方法，以获得马克斯马氏霉属中的蛋白质。 构成：表达载体包含：复制起点; CYC启动子，TEF启动子，GPD启动子或ADH启动子; 和终结者。 CYC启动子含有序列号1的序列或与序列号1的序列具有70％以上序列同源性的序列.TEF启动子具有序列号2的序列或具有70％以上序列同源性的序列 序列号2的序列.GPD启动子含有序列号3的序列或与序列号3的序列具有70％以上同源性的序列.ADH启动子具有序列号4的序列或70％以上的序列 与序列号4的序列同源性。

公开(公告)号：KR1020130000640A

公开(公告)日：2013-01-03

申请号：KR1020110061290

申请日：2011-06-23

Applicant: 삼성전자주식회사

Inventor： 조화영 ,  유병조 ,  박재찬 ,  박성민 ,  구현민 ,  이주영

IPC: C12Q1/04 ,  C12N1/20 ,  C12N1/14

CPC classification number: G01N21/80 ,  C12Q1/045 ,  Y02A50/451

Abstract: PURPOSE: A high throughput screening system and method using a pH indicator are provided to accurately, quickly, and easily screen strains which highly produce acids. CONSTITUTION: A system for screening acid-producing stains contains the acid-producing strains, a medium, two or more pH indicators. The acid includes citric acid, itaconic acid, succinic acid, fumaric acid, glycolic acid, pyruvic acid, acetic acid, glutamic acid, malic acid, maleic acid, 3-hydroxypropionic acid(3-HP), butyric acid, or gluconic acid. The strains are Bacillus sp., Streptococcus sp., Streptomyces sp., Staphylococcus sp., Enterococcus sp., Lactobacillus sp., Lactococcus sp., Clostridium sp., Geobacillus sp., Escherichia. Coli sp., Pseudomonas sp., Salmonella sp., Campylobacter sp., Helicobacter sp., Flavobactenum sp., Fusobacterium sp., llyobacter sp., Neisseria sp., Candida sp., Hansenula sp., Kluyveromyces sp., Pichia sp., Saccharomyces sp., Schizosaccharomyces sp., or Yarrowta sp.

Abstract translation: 目的：提供高通量筛选系统和使用pH指示剂的方法来准确，快速，容易地筛选高度产生酸的菌株。 构成：用于筛选产酸污渍的系统包含酸生产菌株，中等，两种或更多种pH指示剂。 酸包括柠檬酸，衣康酸，琥珀酸，富马酸，乙醇酸，丙酮酸，乙酸，谷氨酸，苹果酸，马来酸，3-羟基丙酸（3-HP），丁酸或葡萄糖酸。 菌株是芽孢杆菌属，链球菌属，链霉菌属，葡萄球菌属，肠球菌属，乳杆菌属，乳球菌属，梭菌属，地芽孢杆菌属，埃希氏菌属。 大肠杆菌，假单胞菌属，沙门氏菌属，弯曲菌属，幽门螺杆菌属，黄杆菌属，福氏杆菌属，丝杆菌属，奈瑟氏球菌属，假丝酵母​​属，汉逊酵母属，克鲁维酵母菌属，毕赤酵母属 ，酵母属（Saccharomyces sp。），粟酒裂殖酵母属（Schizosaccharomyces sp。）或Yarrowta sp。