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公开(公告)号:AU674694B2
公开(公告)日:1997-01-09
申请号:AU4761093
申请日:1993-08-10
Applicant: FUSO PHARMACEUTICAL IND
Inventor: MATSUHISA AKIO , SHIBA KIYOTAKA , MIKAWA YOSHIKAZU , KISHI YUICHIRO
IPC: C12Q1/68 , C12Q1/6813 , C12Q1/6816 , G01N27/447 , G01N33/50 , G01N33/58 , C12Q1/28 , C12Q1/42
Abstract: A method of detecting a nucleic acid which comprises bringing a solid carrier suspected to carry or contain a nucleic acid into contact with a polyamine containing, bound thereto, a label capable of generating a detectable signal or a precursor thereof to thereby form a complex between a nucleic acid, if any, and the polyamine, removing the polyamine which has not formed any complex before or after the conversion of the precursor, if used, into the label, and detecting the label.
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公开(公告)号:AU3618995A
公开(公告)日:1996-04-26
申请号:AU3618995
申请日:1995-10-02
Applicant: FUSO PHARMACEUTICAL IND
Inventor: KESHI HIROYUKI , EDA SOJI , UEHARA HIROTSUGU , UEYAMA HIROSHI , MATSUHISA AKIO
Abstract: A probe derived from phlogogenic bacteria of pneumonia, containing fragments of DNA which streptococcus pneumoniae essentially possesses, and useful for detecting and identifying causative bacteria of pneumonia is obtained by completely digesting the DNA with a restriction endonuclease PstI, followed by cloning into a suitable vector.
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公开(公告)号:CA2555346C
公开(公告)日:2015-04-21
申请号:CA2555346
申请日:2005-02-08
Applicant: FUSO PHARMACEUTICAL IND
Inventor: MATSUHISA AKIO , YAMAMOTO SEIJI , EDA SOUJI , YAMASAKI SHINJI
Abstract: It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then a nucleic acid thus amplified is detected. Since the nucleic acids are not extracted from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.
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公开(公告)号:DE60221126D1
公开(公告)日:2007-08-23
申请号:DE60221126
申请日:2002-05-27
Applicant: FUSO PHARMACEUTICAL IND , OHNO TSUNEYA
Inventor: OHNO TSUNEYA , MATSUHISA AKIO
IPC: C12Q1/68 , C12N15/31 , C12Q1/04 , G01N33/53 , G01N33/566 , G01N33/569
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公开(公告)号:DK1160334T3
公开(公告)日:2007-01-08
申请号:DK01203326
申请日:1993-07-07
Applicant: FUSO PHARMACEUTICAL IND , OHNO TSUNEYA
Inventor: MATSUHISA AKIO , UEHARA HIROTSUGU , EDA SOJI
IPC: C12N15/09 , C12Q1/68 , C12N15/11 , C12R1/01 , C12R1/19 , C12R1/22 , C12R1/385 , G01N33/53 , G01N33/566
Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.
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公开(公告)号:AT346956T
公开(公告)日:2006-12-15
申请号:AT03075759
申请日:1993-07-07
Applicant: FUSO PHARMACEUTICAL IND , OHNO TSUNEYA
Inventor: MATSUHISA AKIO , UEHARA HIROTSUGU , EDA SOJI
IPC: C12N15/09 , C12N15/11 , C12Q1/68 , C12R1/01 , C12R1/19 , C12R1/22 , C12R1/385 , G01N33/53 , G01N33/566
Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.
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公开(公告)号:DE69334080D1
公开(公告)日:2006-12-14
申请号:DE69334080
申请日:1993-07-07
Applicant: FUSO PHARMACEUTICAL IND , OHNO TSUNEYA
Inventor: OHNO TSUNEYA , MATSUHISA AKIO , UEHARA HIROTSUGU , EDA SOJI
IPC: C12N15/09 , C12Q1/68 , C12N15/11 , C12R1/01 , C12R1/19 , C12R1/22 , C12R1/385 , G01N33/53 , G01N33/566
Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.
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公开(公告)号:DE69334018D1
公开(公告)日:2006-06-22
申请号:DE69334018
申请日:1993-07-07
Applicant: FUSO PHARMACEUTICAL IND , OHNO TSUNEYA
Inventor: OHNO TSUNEYA , MATSUHISA AKIO , UEHARA HIROTSUGU , EDA SOJI
IPC: C12N15/09 , C12Q1/68 , C12N15/11 , C12R1/01 , C12R1/19 , C12R1/22 , C12R1/385 , G01N33/53 , G01N33/566
Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.
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公开(公告)号:AT328118T
公开(公告)日:2006-06-15
申请号:AT04077305
申请日:1993-07-07
Applicant: FUSO PHARMACEUTICAL IND , OHNO TSUNEYA
Inventor: OHNO TSUNEYA , MATSUHISA AKIO , UEHARA HIROTSUGU , EDA SOJI
IPC: C12N15/09 , C12N15/11 , C12Q1/68 , C12R1/01 , C12R1/19 , C12R1/22 , C12R1/385 , G01N33/53 , G01N33/566
Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.
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公开(公告)号:DE69333872T2
公开(公告)日:2006-06-14
申请号:DE69333872
申请日:1993-07-07
Applicant: FUSO PHARMACEUTICAL IND , OHNO TSUNEYA
Inventor: OHNO TSUNEYA , MATSUHISA AKIO , UEHARA HIROTSUGU , EDA SOJI
IPC: C12N15/09 , C12Q1/68 , C12N15/11 , C12R1/01 , C12R1/19 , C12R1/22 , C12R1/385 , G01N33/53 , G01N33/566
Abstract: A probe for diagnosing infectious diseases in which the probe comprises the base sequence set out in SEQ. ID. No. 7 and specifically reacts with DNA of Staphylococcus epidermidis.
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