公开(公告)号：DE102005045163B4

公开(公告)日：2007-06-14

申请号：DE102005045163

申请日：2005-09-21

Applicant: LEICA MICROSYSTEMS

Inventor： WIDZGOWSKI BERND ,  BIRK HOLGER

IPC: G02B21/00

Abstract: The microscope has a detector (19) to receive the light emanating from a specimen (15) to generate a detected signal. A digital circuit (30) periodically interrogates the detected signal for each pixel and calculates an average based on the clock frequency. An independent claim is included for fluorescent light detection method.

公开(公告)号：ES2268137T3

公开(公告)日：2007-03-16

申请号：ES02791475

申请日：2002-07-26

Applicant: LEICA MICROSYSTEMS

Inventor： BIRK HOLGER

IPC: G02B21/00 ,  G02F1/11

Abstract: Disposición óptica para la separación espacial de un rayo de luz de iluminación (11) y de un rayo de luz de detección (29), que discurre en dirección opuesta, con un componente acústico-óptico (13) y con un elemento de compensación (31), que compensa una división, provocada por el componente acústico-óptico (13) mediante birrefringencia, del rayo de luz de detección (29) al pasar una vez, y en que el componente acústico-óptico (13) y el elemento de compensación (31) están dispuestos de tal modo que el rayo de luz de iluminación (11) sólo recorre el componente acústico-óptico (13), caracterizada porque el elemento de compensación (31) y el componente acústico-óptico (13) tienen la misma forma exterior, están hechos del mismo material y tienen la misma estructura de cristal, porque el elemento de compensación (31) es otro componente acústico-óptico (33), y porque el elemento de compensación (31) y el componente acústico- óptico (13) están orientados de forma girada en 180 grados entre sí en lo que respecta a la dirección de propagación del rayo de luz de detección (29) que incide sobre el componente acústico-óptico.

公开(公告)号：DE102004054262A1

公开(公告)日：2006-05-11

申请号：DE102004054262

申请日：2004-11-09

Applicant: LEICA MICROSYSTEMS

Inventor： RIEDMANN JUERGEN ,  BOEHM INGO ,  LEIMBACH VOLKER ,  ULRICH HEINRICH ,  BIRK HOLGER

IPC: G02B21/00

公开(公告)号：DE102004049437A1

公开(公告)日：2006-04-27

申请号：DE102004049437

申请日：2004-10-19

Applicant: LEICA MICROSYSTEMS

Inventor： BIRK HOLGER ,  FEHRER DIRK-OLIVER ,  GOLDNER MICHAEL

IPC: G02B21/00

Abstract: The device has a deflecting unit (15) arranged for adjustable deflection of light beams, and including an oscillating reflector and a galvanometer reflector. The deflecting unit is positioned in a sound-proof housing (17) with an entry window (19) and/or exit window (21), where the exit window is designed as a lens (23). The housing has lining with an acoustical absorption unit (27). An independent claim is also included for a confocal scanning microscope with a beam deflecting device.

公开(公告)号：DE102004038321A1

公开(公告)日：2005-06-02

申请号：DE102004038321

申请日：2004-08-06

Applicant: LEICA MICROSYSTEMS

Inventor： BOEHM INGO ,  BIRK HOLGER ,  LEIMBACH VOLKER ,  SEIFERT ROLAND

IPC: G02B5/00 ,  G02B5/10

Abstract: A light trap (1) comprises a hollow element (3) for absorbing undesirable light, and a feed element (5) opening into the hollow element and guiding undesirable light into the hollow element. Each imaginary ray leading inward from the inner surface (7) of the hollow element intersects the inner surface of the hollow element and/or the inner surface (9) of the feed element. An independent claim is also included for a microscope, especially a scanning microscope or a confocal scanning microscope, comprising the above light trap.

公开(公告)号：DE10324331A1

公开(公告)日：2004-12-30

申请号：DE10324331

申请日：2003-05-27

Applicant: LEICA MICROSYSTEMS

Inventor： MOELLMANN KYRA ,  BIRK HOLGER

IPC: G01J1/42 ,  G02B21/00 ,  G02B21/06 ,  G02F1/33

Abstract: The apparatus has a detector (39) receiving a detection light that is lead along a detection beam path from a sample. An acousto-optical unit (13) is disposed in the illumination beam path and driven by a high-frequency source (57). A measuring device (55) measures an optical power. A control unit (53) controls the high-frequency source as a function of the measured power so as to achieve a selectable level of the power. An independent claim is also included for a method of controlling an optical power in a confocal laser scanning microscope.

公开(公告)号：DE10319946A1

公开(公告)日：2004-11-18

申请号：DE10319946

申请日：2003-05-02

Applicant: LEICA MICROSYSTEMS

Inventor： BIRK HOLGER ,  STORZ RAFAEL

IPC: G01N21/64

Abstract: Method for classification of image areas (1) that have been obtained by imaging an object using a confocal scanning microscope and whereby the object has been marked with various markers. At least two detection channels are used to detect light of different intensities originating from the markers and for generating a light intensity dependent signal. The object image areas are classified based on the ratios of the detection signals.

公开(公告)号：DE10317669A1

公开(公告)日：2004-11-04

申请号：DE10317669

申请日：2003-04-17

Applicant: LEICA MICROSYSTEMS

Inventor： STORZ RAFAEL ,  BIRK HOLGER

IPC: C12M1/34 ,  C12Q1/68 ,  C12Q1/70 ,  G01J3/14 ,  G01J3/28 ,  G01J3/36 ,  G01N21/64 ,  G02B21/00

Abstract: A method for separating detection channels is disclosed, a sample (15) being equipped with at least two different fluorescent dyes. Firstly the emission spectrum of at least two fluorescent dyes is ascertained. From the emission spectra, the separation points of the wavelength and of the individual detection channels are determined. Lastly, adjustment of the separation of the at least two channels is accomplished on that basis.

公开(公告)号：AU2003280287A1

公开(公告)日：2004-06-23

申请号：AU2003280287

申请日：2003-09-23

Applicant: LEICA MICROSYSTEMS

Inventor： BIRK HOLGER ,  STORZ RAFAEL ,  KNEBEL WERNER

IPC: G02B21/00

公开(公告)号：GB2368743B

公开(公告)日：2003-03-26

申请号：GB0120663

申请日：2001-08-24

Applicant: LEICA MICROSYSTEMS

Inventor： STORZ RAFAEL ,  ENGELHARDT JOHANN ,  BIRK HOLGER

IPC: G02B26/06 ,  G02B21/00 ,  G02B21/06 ,  G02F1/01 ,  H01S3/00

Abstract: A method for illuminating an object with light (2) from a laser light source (3), preferably in a confocal scanning microscope (1). With the method according to the invention, it is possible to reduce the coherence length of the laser light, so that disruptive interference phenomena can be substantially eliminated. Should interference phenomena nevertheless be formed, these are to be influenced in such a way that they have no effect on the detection. The method according to the invention is characterized in that the phase angle of the light field is varied by a modulator in such a way that interference phenomena do not occur in the optical beam path, or occur only to an undetectable extent, within a predeterminable time interval.