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    개 인플루엔자 ＶＬＰ 백신의 제조방법
    73.
    发明公开
    개 인플루엔자 ＶＬＰ 백신의 제조방법 有权
    病毒样颗粒疫苗对犬流行性感冒病毒的制备方法

    公开(公告)号：KR1020140052408A

    公开(公告)日：2014-05-07

    申请号：KR1020120118487

    申请日：2012-10-24

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 정혜영 ,  안동준 ,  임성인 ,  송재영

    IPC: A61K48/00 ,  A61K39/145 ,  A61K38/17 ,  A61K38/16

    CPC classification number: A61K48/0066 ,  A61K39/145 ,  Y10S424/818

    Abstract: The present invention relates to a manufacturing method of a vaccine against canine influenza virus capable of preventing and/or treating canine influenza virus and, more specifically, to a manufacturing method of a VLP (virus-like particle) vaccine against canine influenza virus, to the VLP vaccine against canine influenza virus manufactured by the method, and to a method for preventing canine influenza virus by inoculating dogs with the VLP vaccine against canine influenza virus manufactured by the manufacturing method. The manufacturing method of the VLP vaccine against canine influenza virus comprises: a step for amplifying hemagglutinin (HA) genes of the canine influenza virus with a sequence number 1 by a forward primer with a sequence number 2 and a reverse primer with a sequence number 3, and for PCR-amplifying matrix 1 (M1) genes of the canine influenza virus with a sequence number 4 by a forward primer with a sequence number 5 and a reverse primer with a sequence number 6; a step for cloning the amplified HA genes and M1 genes to a vector; and a step for VLP-manifesting the vector cloned with the amplified HA genes and M1 genes in insect cells.

    Abstract translation: 本发明涉及能够预防和/或治疗犬流感病毒的针对犬流感病毒的疫苗的制造方法，更具体地，涉及针对犬流感病毒的VLP（病毒样颗粒）疫苗的制造方法， 针对通过该方法制造的犬流感病毒的VLP疫苗，以及通过用针对通过制造方法制造的犬流感病毒的VLP疫苗接种狗来预防犬流感病毒的方法。 针对犬流感病毒的VLP疫苗的制造方法包括：通过序列号为2的正向引物和序列号3的反向引物扩增序列号为1的犬流感病毒血凝素（HA）基因的步骤 ，以及序列号为5的正向引物的序列号为6的犬流感病毒的PCR扩增基因1（M1）基因和序列号为6的反向引物; 将扩增的HA基因和M1基因克隆到载体上的步骤; 以及用昆虫细胞中扩增的HA基因和M1基因克隆的载体的VLP表现的步骤。

    소설사병 바이러스성 원인체의 유전자 진단을 위한 프로브, 프라이머 및 이를 이용한 진단방법
    75.
    发明公开
    소설사병 바이러스성 원인체의 유전자 진단을 위한 프로브, 프라이머 및 이를 이용한 진단방법 有权
    用于NDC病毒病原体基因诊断的探针和引物及使用其的诊断方法

    公开(公告)号：KR1020130058105A

    公开(公告)日：2013-06-04

    申请号：KR1020110123936

    申请日：2011-11-25

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 안동준 ,  정우석 ,  정혜영 ,  주후돈 ,  김준배 ,  유은정 ,  김은미

    IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

    CPC classification number: Y02A50/451 ,  C12Q1/6883 ,  C12Q1/04 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q2600/124

    Abstract: PURPOSE: A probe and a gene chip for diagnosing norovirus, rotavirus, and coronavirus, a primer for amplifying a gene, and a method for diagnosing pathogen using the same are provided to detect pathogen causing NCD(Neonatal Calf Diarrhea) and genotype. CONSTITUTION: A primer for detecting rotavirus, norovirus, and coronavirus which are a pathogen causing NCD contains a primer containing one or more sequences among sequence numbers 1-12. A method for amplifying a gene comprises simultaneously detecting rotavirus A, B, and C using a primer containing sequences of sequence numbers 1-6. The method comprises a step of detecting norovirus genotype group I, II, and III using a primer containing sequences of sequence numbers 7-10. The method comprises a step of detecting coronavirus using one or more primers among sequence numbers of sequence numbers 11 and 12. A probe contains one or more sequences among sequence numbers 13-91 to detect rotavirus, norovirus, and coronavirus.

    Abstract translation: 目的：提供用于诊断诺如病毒，轮状病毒和冠状病毒的探针和基因芯片，用于扩增基因的引物和使用其的病原体诊断方法，以检测引起NCD（新生小牛腹泻）和基因型的病原体。 构成：用于检测导致NCD的病原体的轮状病毒，诺如病毒和冠状病毒的引物含有含有序列号1-12中的一个或多个序列的引物。 用于扩增基因的方法包括使用含有序列号1-6的序列的引物同时检测轮状病毒A，B和C. 该方法包括使用含有序列号7-10的序列的引物检测诺如病毒基因型I，II和III的步骤。 该方法包括使用序列号11和12的序列号中的一种或多种引物检测冠状病毒的步骤。探针含有序列号13-91中的一个或多个序列，以检测轮状病毒，诺如病毒和冠状病毒。

    재조합 단백질을 항원으로 이용한 소 바이러스성 설사증의진단방법
    77.
    发明授权
    재조합 단백질을 항원으로 이용한 소 바이러스성 설사증의진단방법 有权
    使用重组蛋白作为抗原的牛病毒性腹泻的诊断方法

    公开(公告)号：KR100331176B1

    公开(公告)日：2002-04-06

    申请号：KR1019990057805

    申请日：1999-12-15

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 현방훈 ,  송재영 ,  안동준 ,  박종현 ,  차상호 ,  안수환

    IPC: A61K39/15

    Abstract: 본발명은국내소 사육농가에호흡기질병및 송아지설사를일으켜심각한피해를주는질병중의하나인소 바이러스성설사증(BVD)에대한진단법에관한것이고, 더욱상세하게는곤충세포에서발현된소 바이러스성설사증바이러스 gp53 단백질로이루어진재조합단백질을항원으로이용하여, 간접결합효소면역측정법으로중화항체가를검출하여바이러스성설사증을진단하는방법에관한것이다. 본발명에따른진단방법은수행시특별한시설및 기술없이도신속하게소 바이러스성설사증바이러스에대한항체를검사할수 있어신속한면역수준검사에의한소 바이러스성질병의예방및 방제대책수립에이용할수 있고, 또한백신접종후 면역수준또는감염실태파악에필요한대량의검사혈청을단시간내에처리할수 있는방법으로소 바이러스성설사증바이러스에의한호흡기병예방및 근절에크게기여할것이다.

    돼지 유행성설사병 바이러스의 특이 중화항체 검출방법
    78.
    发明授权
    돼지 유행성설사병 바이러스의 특이 중화항체 검출방법 有权
    中毒抗疟原虫抗病毒药物的检测方法

    公开(公告)号：KR100267745B1

    公开(公告)日：2000-11-01

    申请号：KR1019980000447

    申请日：1998-01-10

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 박종현 ,  송재영 ,  안동준 ,  현방훈 ,  차상호 ,  안수환 ,  권준헌

    IPC: C12N15/33

    Abstract: PURPOSE: Provided is a spike gene and a method for detecting neutralizing antibodies of porcine epidemic diarrhea virus to prevent porcine epidemic diarrhea by using spike proteins which are mass-produced through genetic engineering. CONSTITUTION: The spike gene obtained from porcine epidemic diarrhea virus is located in the gene of recombinant baculovirus (KFCC 11014). And a method for detecting neutralizing antibodies of porcine epidemic diarrhea virus (PEDV) is characterized by the next step of: using a spike protein expressed in recombinant baculovirus (KFCC 11014) as an antigen in implementing indirect-ELISA. A method for manufacturing the recombinant baculovirus (KFCC 11014) is comprised of the following steps of: i) making expression vector pVL 1393 having a spike gene of PEDV and obtaining a medium lacking serum but including linear baculovirus, and mixture containing lipopectin; and ii) cotransfecting host cell Sf9 using the mixture.

    Abstract translation: 目的：提供一种尖峰基因和一种检测猪流行性腹泻病毒中和抗体的方法，通过使用通过基因工程大规模生产的穗蛋白来预防猪流行性腹泻。 构成：从猪流行性腹泻病毒获得的穗基因位于重组杆状病毒（KFCC 11014）的基因中。 检测猪流行性腹泻病毒（PEDV）中和抗体的方法的特征在于：使用重组杆状病毒（KFCC 11014）中表达的尖峰蛋白作为抗原进行间接ELISA。 制备重组杆状病毒（KFCC 11014）的方法包括以下步骤：i）制备具有PEDV刺突基因的表达载体pVL1393，获得缺乏血清但包括线性杆状病毒的培养基，以及含有脂蛋白的混合物; 和ii）使用该混合物共转染宿主细胞Sf9。

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