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    METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR CALCULATING CORRECTED AMPLICON COVERAGES
    82.
    发明申请
    METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR CALCULATING CORRECTED AMPLICON COVERAGES 审中-公开
    用于计算校正的AMPLICON封面的方法，系统和计算机可读介质

    公开(公告)号：WO2016057902A1

    公开(公告)日：2016-04-14

    申请号：PCT/US2015/054910

    申请日：2015-10-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： VEITCH, James ,  ZHAN, Yiping

    IPC: G06F19/18 ,  G06F19/20 ,  G06F19/22

    CPC classification number: G06F19/24 ,  C12Q1/6869 ,  G06F19/18 ,  G06F19/22

    Abstract: Methods, systems, and computer-readable media are disclosed for calculating corrected amplicon coverages. One method includes: mapping a plurality of reads of a plurality of amplicons based on amplified target regions of a sample suspected of having one or more genetic abnormalities to a reference sequence that includes one or more nucleic acid sequences corresponding to the amplified target regions; calculating amplicon coverages and total reads, wherein amplicon coverages is a number of reads mapped to an amplicon, and total reads is a number of mapped reads; and calculating corrected amplicon coverages based on the calculated amplicon coverages and calculated total reads by applying a batch effect correction.

    Abstract translation: 公开了用于计算校正的扩增子覆盖物的方法，系统和计算机可读介质。 一种方法包括：基于被怀疑具有一个或多个遗传异常的样本的扩增的目标区域将多个扩增子的多个读取映射到包括与扩增的目标区域相对应的一个或多个核酸序列的参考序列; 计算扩增子覆盖率和总读数，其中扩增子覆盖是映射到扩增子的多个读取，并且总读取是映射读取的数量; 并且通过应用批量效应校正，基于计算的扩增子覆盖率和计算的总读数计算校正的扩增子覆盖率。

    REVERSIBLY INACTIVATED THERMOSTABLE REVERSE TRANSCRIPTASES, COMPOSITIONS AND METHODS FOR USE
    84.
    发明申请
    REVERSIBLY INACTIVATED THERMOSTABLE REVERSE TRANSCRIPTASES, COMPOSITIONS AND METHODS FOR USE 审中-公开
    可逆的可逆反转转录物，组合物和使用方法

    公开(公告)号：WO2016033116A1

    公开(公告)日：2016-03-03

    申请号：PCT/US2015/046811

    申请日：2015-08-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： ROGERS, Jeffrey ,  LAM, Bianca ,  GUO, Joanna ,  LI, Lushen

    IPC: C12N9/99 ,  C12N9/12

    CPC classification number: C12N9/1276 ,  C12N9/12 ,  C12N9/99 ,  C12P19/34 ,  C12Y207/07049

    Abstract: The present invention provides reversibly inactivated reverse transcriptase enzymes, particularly those having increased thermostability and/or thermoreactivity and compositions, methods and kits that include such enzymes, for the reverse transcription of nucleic acid molecules. Also provided are compositions and methods for the reactivation of reversibly inactivated reverse transcriptases. More particularly, the present invention relates to compositions and methods that can increase the speed of reactivation and stabilize reactivation of chemically modified reverse transcriptase enzymes prior to, or as part of a reverse transcription reaction. As compared to existing compositions and methods, the reversibly inactivated reverse transcriptase enzymes of the present invention provide for a significant reduction in non-specific reverse transcription from template nucleic acid molecules.

    Abstract translation: 本发明提供了可逆地灭活的逆转录酶，特别是那些具有增加的热稳定性和/或热反应性的组合物，包含这些酶的组合物，方法和试剂盒，用于逆转录核酸分子。 还提供了用于重新激活可逆失活的逆转录酶的组合物和方法。 更具体地说，本发明涉及可以在逆转录反应之前或作为逆转录反应的一部分期间增加再活化速度并稳定化学修饰的逆转录酶的再活化的组合物和方法。 与现有的组合物和方法相比，本发明的可逆失活的逆转录酶提供了模板核酸分子的非特异性逆转录的显着降低。

    DIRECT QUANTITATIVE PCR ABSENT MINOR GROOVE BINDERS
    85.
    发明申请
    DIRECT QUANTITATIVE PCR ABSENT MINOR GROOVE BINDERS 审中-公开
    直接定量PCR不育小孔蛋白粘合剂

    公开(公告)号：WO2016003977A1

    公开(公告)日：2016-01-07

    申请号：PCT/US2015/038444

    申请日：2015-06-30

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LIU, Jason Yingjie

    IPC: C12Q1/68

    CPC classification number: C12Q1/701 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2531/113 ,  C12Q2561/113

    Abstract: Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support such as filter paper. This entails quantitative real - time polymerase chain reaction (qrt PCR) performed directly on the filter paper, wherein minor groove binders (MGBs) are excluded from the structure of the fluorescently labelled probe and thus from the reaction. The absence of the MGBs should improve the background fluorescence in real-time PCR.

    Abstract translation: 本文公开了用于定量存在于固体支持物如滤纸上的核酸靶的方法，组合物和试剂盒。 这需要直接在滤纸上进行的定量实时聚合酶链反应（qrt PCR），其中从荧光标记的探针的结构中排除小沟结合物（MGB），从而从反应中排除。 MGBs的缺失应提高实时PCR中的背景荧光。

    SEQUENCING DEVICE
    86.
    发明申请
    SEQUENCING DEVICE 审中-公开
    顺序设备

    公开(公告)号：WO2015195831A1

    公开(公告)日：2015-12-23

    申请号：PCT/US2015/036277

    申请日：2015-06-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： SCHULTZ, Jonathan ,  ROSWECH, Todd ,  HOSHIZAKI, Jon ,  CARRILLO, Albert ,  BALL, James

    IPC: B01L3/00 ,  G01N35/10

    CPC classification number: C12Q1/6869 ,  B01F1/0027 ,  B01F5/0683 ,  B01F11/0048 ,  B01F11/0071 ,  B01L3/502 ,  B01L3/505 ,  B01L3/527 ,  B01L3/563 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683 ,  C12Q1/6806 ,  G01N27/4145 ,  G01N35/1002 ,  G01N35/1095 ,  Y10T436/11 ,  Y10T436/143333

    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

    Abstract translation: 制备试剂的方法包括将药筒插入仪器中。 盒包括设置在盒的空腔中并将端口暴露于盒的外部的多个试剂盒。 每个试剂盒包括试剂容器，其包括试剂和限定可压缩体积的内腔，通过试剂容器限定到内腔的开口。 该方法还包括将多个流体端口连接到多个试剂盒的开口; 通过所述流体端口施加溶液以至少部分地填充所述多个试剂外壳; 并且循环空腔的压力，由此对于每个试剂外壳，在增加的压力期间，溶液进入试剂容器的内腔，与试剂结合，并压缩可压缩体积，并且在减压期间，可压缩体积 减少，试剂通过开口排出。

    SYSTEMS AND METHODS FOR SUBSTRATE ENRICHMENT
    88.
    发明申请
    SYSTEMS AND METHODS FOR SUBSTRATE ENRICHMENT 审中-公开
    用于基层增厚的系统和方法

    公开(公告)号：WO2015191877A1

    公开(公告)日：2015-12-17

    申请号：PCT/US2015/035366

    申请日：2015-06-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： REED, Brian

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12N15/1013 ,  C12Q1/6844 ,  C12Q2537/163 ,  C12Q2563/159

    Abstract: A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. The unbound polynucleotide includes a segment complementary to a coupling oligonucleotide. The first set of bead substrates includes the coupling oligonucleotide extended to include a segment complementary to a portion of the unbound polynucleotide. The second set of bead substrates includes the coupling oligonucleotide. The emulsion-breaking solution includes an interference probe having a sequence similar to the coupling oligonucleotide or complementary to the coupling oligonucleotide. The method further includes binding beads of the first set of bead substrates to separation substrates and separating unbound beads of the second set of bead substrates from the beads of the first set of bead substrates bound to the separation substrates.

    Abstract translation: 分离珠粒基材的方法包括将乳液施加到破乳溶液中。 乳液的分散相包括未结合的多核苷酸，第一组珠粒基质和第二组珠粒基底。 未结合的多核苷酸包括与偶联寡核苷酸互补的区段。 第一组珠底物包括延伸至包含与未结合多核苷酸的一部分互补的区段的偶联寡核苷酸。 第二组珠底物包括偶联寡核苷酸。 破乳溶液包括具有与偶联寡核苷酸相似的序列或与偶联寡核苷酸互补的序列的干扰探针。 该方法还包括将第一组胎圈衬底的珠粒与分离衬底结合，并将第二组胎圈衬底的未结合的珠粒与与分离衬底结合的第一组胎圈衬底的珠粒分离。

    METHODS AND SYSTEMS FOR PCR QUANTITATION
    89.
    发明申请
    METHODS AND SYSTEMS FOR PCR QUANTITATION 审中-公开
    PCR定量的方法和系统

    公开(公告)号：WO2015153973A1

    公开(公告)日：2015-10-08

    申请号：PCT/US2015/024249

    申请日：2015-04-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： WU, Yalei ,  KEYS, David

    IPC: C12Q1/68

    CPC classification number: G06F19/24 ,  C12Q1/6851 ,  G06F19/12 ,  G06F19/20 ,  G06F19/22 ,  G06F19/26 ,  C12Q2537/16 ,  C12Q2545/114

    Abstract: A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.

    Abstract translation: 提供了定量核酸的方法。 该方法包括确定用于参考核酸的第一预定输入量的第一参考阈值循环，确定靶核酸的第一预定输入量的第一目标阈值循环，为第二预定输入确定第二参考阈值循环 参考核酸的量，以及由处理器确定靶核酸的第二预定输入量的第二目标阈值循环。 该方法还包括接收采样阈值周期，基于第一和第二参考阈值周期以及第一和第二目标阈值周期来确定采样输入量，以及将样本输入量显示给用户。

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