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公开(公告)号：KR1020100059668A
公开(公告)日：2010-06-04
申请号：KR1020090075672
申请日：2009-08-17
Applicant: 건국대학교 산학협력단 , 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C07K14/195 , C12N1/20 , C12P21/00
Abstract: PURPOSE: A bacteriocin which is derived from Lactococcus lactis NK34 KFCC 11458P and a therapeutic agent for breast cancer using the same are provided to ensure antibacterial activity and to be used as an antibiotic substitute. CONSTITUTION: A Lactcin NK34 enzyme is isolated from Lactococcus lactis NK34 KFCC 11458P and is used for treating breast cancer of dairy cattle. The Lacticin NK34 has an antibacterial effect to S. uberis E290, S. agalactiae ATCC 13813, E. gallinarum E362, E. faecium E363, E. faecium E374 and P. autotrophica KCTC 9455. A method for isolating bacteriocin Lacticin NK34 enzyme comprises: a step of isolating and culturing using a strain; a step of measuring antibacterial activity of the bacteriocin; a step of researching the characteristics of Lactin NK 34 enzyme; and a step of measuring antibacterial effects to a breast cancer pathogenic bacteria.
Abstract translation: 目的：提供来源于乳酸乳球菌NK34 KFCC 11458P的细菌素和使用其的乳腺癌治疗剂以确保抗菌活性并用作抗生素替代物。 构成：乳酸菌NK34酶从乳酸乳球菌NK34 KFCC 11458P分离，用于治疗乳牛的乳腺癌。 乳酸NK34具有对乳房链球菌E290，无乳链球菌ATCC 13813，gall藜E362，屎肠球菌E363，屎肠球菌E374和自交亲属KCTC 9455的抗菌作用。一种分离细菌素Lacticin NK34酶的方法，包括： 使用菌株分离培养的步骤; 测定细菌素的抗菌活性的步骤; 研究乳酸NK34酶的特征的一个步骤; 以及测定对乳腺癌病原菌的抗菌作用的步骤。
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82.发明授权면역특이단백질 항원을 사용하는 우결핵 특이 효소면역진단키트 및 이를 이용한 우결핵 진단방법 有权包含免疫特异性蛋白质抗原和其他免疫原的牛结核特异性酶免疫测定试剂盒及其使用相同的牛结核病诊断方法
公开(公告)号：KR100951057B1
公开(公告)日：2010-04-07
申请号：KR1020070118743
申请日：2007-11-20
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 우결핵 특이 효소면역진단 키트 및 이를 이용한 우결핵 진단방법에 관한 것으로, 보다 상세하게는 우결핵균의 단백질항원 중 면역특이단백질 항원인 MPB70 등을 이용한 효소면역측정법을 통하여 신속하고 정확하게 우결핵을 진단하는 우결핵 특이 효소면역진단 키트 및 이를 이용한 우결핵 진단방법에 관한 것이다.
우결핵, 정제단백질, 효소면역측정법, 피내검사-
公开(公告)号：KR1020090114665A
公开(公告)日：2009-11-04
申请号：KR1020080040422
申请日：2008-04-30
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/48 , G01N33/52 , G01N33/543
Abstract: PURPOSE: A diagnostic latex liquid and a simple method for diagnosing laptospira are provided to quickly confirm infection of laptospira and minimize the infection. CONSTITUTION: A diagnostic latex liquid using laptospira serum sejroe is prepared by crushing the laptospira serum sejroe by ultrasonic wave and adhering to latex bead. An antigen used for latex diagnosis is laptospira serum sejroe. The crushing is performed at 10-60 KHz for 10 to 30 minutes. A method for diagnosing laptospira comprises: a step of mixing same amount of test serum with 30ul of latex liquid on glass plate; a step of shaking the glass plate and read formation of aggregation; and a step of diagnosing that the formation of specific aggregation is laptospira positive and no aggregation is laptospira negative.
Abstract translation: 目的：提供诊断乳液液体和简单的诊断Laptospira方法，以快速确认laptospira感染并尽量减少感染。 构成：使用laptospira血清sejroe的诊断胶乳液通过超声波粉碎laptospira血清sejroe并粘附到乳胶珠制备。 用于乳胶诊断的抗原是laptospira血清sejroe。 破碎在10-60 KHz进行10〜30分钟。 一种诊断眼线笔的方法包括：将相同量的血清与30ul胶乳液混合在玻璃板上的步骤; 摇晃玻璃板并阅读聚集的步骤; 诊断特定聚集体形成为laptospira阳性并且没有聚集是laptospira阴性的步骤。
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84.发明公开동물의 면역능을 증강시키는 벌나무가지 추출물 및 이를함유하는 면역증강제 无效用于改善动物免疫的ACER TEGMENTOSUM MAXIM RAMULUS的提取物和用于改善含有其的免疫的试剂
公开(公告)号：KR1020090021610A
公开(公告)日：2009-03-04
申请号：KR1020070086250
申请日：2007-08-27
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: Provided is an immunity improver using acer tegmentosum maximowocz extract which improves a function of a nonspecific defense against pathogen and increases activity of animal immunocytes such as neutrophil and macrophages. An immunity improver using acer tegmentosum maximowocz extract comprises acer tegmentosum maximowocz extract as an active ingredient. A method for preparing the acer tegmentosum maximowocz extract includes a step of extracting the extract from the dried acer tegmentosum maximowocz at a temperature of 90~100°C by hot distilled-water.
Abstract translation: 本发明提供了一种免疫改良剂，其使用宏碁提取物，其改善对病原体的非特异性防御的功能，并增加动物免疫细胞如嗜中性粒细胞和巨噬细胞的活性。 使用槭树提取物的抗微生物改良剂作为活性成分。 本发明提供了一种用于制备尖角果胶提取物的方法，其特征在于，在90〜100℃的温度下，通过热蒸馏水从干燥的青蒿中提取提取物。
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公开(公告)号：KR1020090013599A
公开(公告)日：2009-02-05
申请号：KR1020070077854
申请日：2007-08-02
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/02 , A61P37/02 , A61K39/085
CPC classification number: A61K39/05 , C12R1/47 , Y10S435/887 , Y10S514/885
Abstract: An immunostimulant containing the lystic extract of Streptomyces albus is provided to reinforce the functions of various immune cells having the major role in the disease defense, thereby increasing the specific and nonspecific resistance to the disease infection of animals. The immunostimulant contains the lystic extract of Streptomyces albus which is prepared by cultivating Streptomyces albus in yeast malt extract agar broth at 26 deg. C for 72 hours, centrifuging the cultured fluid at 3000Xg for 30 minutes and collecting it, rinsing the collected pellet with phosphate buffered saline(PBS) of pH 7.2 and suspending the rinsed pellet in PBS, adding 5% of TNT solution containing 50mM of Tris-HCl, 0.15M of NaCl and 4% Triton x-114 as solubilizing agent into the Streptomyces albus suspension, stirring the mixture at room temperature for 2-3 days to solubilize the Streptomyces albus, and regulating pH of the solution to 6.5-7.2, and drying the solution in the thermostatic chamber.
Abstract translation: 提供含有链霉菌（Streptomyces albus）的提取物的免疫刺激剂，以增强在疾病防御中具有主要作用的各种免疫细胞的功能，从而增加对动物疾病感染的特异性和非特异性抗性。 免疫刺激剂含有通过在26度的酵母麦芽提取物琼脂培养基中培养链霉菌（Streptomyces albus）而制备的链霉菌链霉菌的提取物。 培养72小时，将培养液以3000Xg离心30分钟并收集，用pH7.2的磷酸盐缓冲盐水（PBS）冲洗收集的沉淀物，并将漂洗的沉淀悬浮于PBS中，加入5％含有50mM Tris -HCl，0.15M NaCl和4％Triton x-114作为增溶剂加入到Streptomyces albus悬浮液中，在室温下搅拌混合物2-3天以溶解链霉菌，并将溶液的pH调节至6.5-7.2 ，并干燥恒温室中的溶液。
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Patent Agency Ranking
公开(公告)号：KR1020090004023A
公开(公告)日：2009-01-12
申请号：KR1020070067899
申请日：2007-07-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/569 , G01N33/53
Abstract: A method for diagnosing the infection of vancomycin-resistant Enterococcus is provided to allow whether vancomycin-resistant Enterococcus is infected or not rapidly and effectively. A method for diagnosing the infection of vancomycin-resistant Enterococcus uses the antigen-antibody reaction with the specific antigen of vancomycin-resistant Enterococcus. Preferably the vancomycin-resistant Enterococcus is any one selected from Vancomycin-resistant Enterococcus phythium (VREFM), Vancomycin-resistant Enterococcus faecalis (VREFS), Vancomycin-resistant Enterococcus gallinarum (VREG), and Vancomycin-resistant Enterococcus casseliflavus.
Abstract translation: 提供了用于诊断耐万古霉素肠球菌感染的方法，以使万古霉素耐药肠球菌感染或不迅速有效地感染。 用于诊断耐万古霉素肠球菌的感染的方法使用与万古霉素耐药性肠球菌的特异性抗原的抗原 - 抗体反应。 优选地，万古霉素耐药性肠球菌是选自万古霉素耐药性肠球菌（VREFM），万古霉素耐药性粪肠球菌（VREFS），万古霉素耐药性肠球菌（VREG）和万古霉素耐药性肠球菌的任何一种。
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公开(公告)号：KR1020080093488A
公开(公告)日：2008-10-22
申请号：KR1020070037227
申请日：2007-04-17
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K36/233 , A61P37/00
Abstract: An extract of Bupleuri Radix is provided to increase various activities of main immunocytes of an animal such as granulocyte and macrophage and induce strong defence capability on attach inoculation of pathogenic bacteria. A composition comprising the same extract is provided to be used as an animal medicine such as an animal immuno-enhancing agent and a vaccine adjuvant and a feed additive for treating and preventing animal diseases efficiently. A method for purifying an extract of Bupleuri Radix comprises the steps of: (a) heat-treating the Bupleuri Radix with hot water at a temperature of 80-120 deg.C for 2-3 hours to obtain a crude extract; (b) concentrating the crude extract; and (c) filtering the concentrate to obtain a polysaccharide fraction by filtering paper and microorganism filtering. An extract of Bupleuri Radix purified by the method is a Bupleuri Radix polysaccharide, of which sugar is a glycoprotein including a protein. A composition for increasing immunity comprises the extract of Bupleuri Radix as an active ingredient and a pharmaceutically acceptable carrier or an excipient. An animal immunity enhancing agent, a vaccine adjuvant, or a feed additive for an animal comprises the composition for increasing immunity as an active ingredient.
Abstract translation: 提供柴胡提取物，以增加动物如粒细胞和巨噬细胞的主要免疫细胞的各种活性，并在附着接种病原菌时诱导强大的防御能力。 提供包含相同提取物的组合物用作动物药物，例如动物免疫增强剂和疫苗佐剂和用于有效治疗和预防动物疾病的饲料添加剂。 纯化柴胡提取物的方法包括以下步骤：（a）在80-120℃的温度下用热水热处理柴胡，2-3小时，得到粗提物; （b）浓缩粗提物; 和（c）过滤浓缩物以通过滤纸和微生物过滤获得多糖级分。 通过该方法纯化的柴胡提取物是一种柴胡甘草多糖，其中糖是包含蛋白质的糖蛋白。 用于增加免疫力的组合物包含作为活性成分的柴胡提取物和药学上可接受的载体或赋形剂。 动物免疫增强剂，疫苗佐剂或用于动物的饲料添加剂包括用于增加免疫力作为活性成分的组合物。
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公开(公告)号：KR1020080092007A
公开(公告)日：2008-10-15
申请号：KR1020070035257
申请日：2007-04-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K36/804 , A61P17/00
CPC classification number: Y02A50/471
Abstract: An extract of Rehmannia glutinosa is provided to increase various activities of neutrophil or macrophages and induce strong protectivity on attack inoculation of pathogenic bacteria, thereby being used as an immune enhancing agent, a vaccine adjuvant, an adjuvant therapeutic agent and a feed additive for an animal. An extract of Rehmannia glutinosa enhances immunity of an animal by increasing the activity of immunocyte such as neutrophil or macrophages. A method for preparing the extract of Rehmannia glutinosa comprises the steps of: (a) boiling dried Rehmannia glutinosa with distilled water to obtain the extract of Rehmannia glutinosa; (b) filtering the extract; and (c) hydrolyzing a filtrate.
Abstract translation: 提供地黄提取物以增加嗜中性粒细胞或巨噬细胞的各种活性，并在致病细菌的侵袭接种中诱发强的保护作用，从而用作免疫增强剂，疫苗佐剂，佐剂治疗剂和用于动物的饲料添加剂 。 地黄提取物通过增加免疫细胞如嗜中性粒细胞或巨噬细胞的活性来增强动物的免疫力。 一种制备地黄提取物的方法，包括以下步骤：（a）用蒸馏水煮沸干燥的地黄，得到地黄提取物; （b）过滤提取物; 和（c）水解滤液。
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公开(公告)号：KR1020080084384A
公开(公告)日：2008-09-19
申请号：KR1020070026062
申请日：2007-03-16
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K36/238 , A61P37/02
Abstract: An extract of Saposhnikovia root is provided to improve immune response by increasing activities of neutrophil, macrophage and lymphocyte, production of cytokines and non-specific protection against pathogenic bacteria infection in animals, so that it is useful as immunomodulator and immunoadjuvant. An extract of Saposhnikovia root for improving immune response in animals is prepared by heating Saposhnikovia root in water, and administered into an animal, especially domestic animals including cow, pig and chicken in the amount of 10-20 mg/kg per day through oral administration, intravenous injection, intramuscular injection or subcutaneous injection.
Abstract translation: 提供Saposhnikovia根的提取物，通过增加嗜中性粒细胞，巨噬细胞和淋巴细胞的活性，产生细胞因子和非特异性保护免受动物中的致病细菌感染来改善免疫应答，从而可用作免疫调节剂和免疫佐剂。 通过在水中加热山茱萸碱来制备Saposhnikovia根的提取物，并通过口服给药至每天10-20mg / kg的动物，特别是包括牛，猪和鸡的家畜，其量为10-20mg / kg ，静脉注射，肌内注射或皮下注射。
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公开(公告)号：KR100850365B1
公开(公告)日：2008-08-05
申请号：KR1020070015656
申请日：2007-02-15
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/54 , A61K2236/331 , Y10S514/885
Abstract: A method for extracting Cinnamoni ramulus is provided to improve extraction yield of useful ingredients from Cinnamoni ramulus, so that the extract of Cinnamoni ramulus enhances immunity of animals, efficacy of vaccine and therapeutic effects of drugs. A method for extracting Cinnamoni ramulus comprises the steps of: mixing dried Cinnamoni ramulus with distilled water in a weight ratio of 1:4; heating the mixture to concentrate the solution; filtering the concentrated extract of Cinnamoni ramulus with filter paper; and filtering the firstly filtered solution with 0.45mum filter paper.
Abstract translation: 提供肉桂提取物提取方法，以提高肉桂樟脑中有用成分的提取率，从而提高肉桂的提取物，增强动物的免疫力，疫苗的疗效和药物的治疗效果。 一种提取肉桂樟脑的方法，包括以下步骤：将干果肉与蒸馏水混合，重量比为1：4; 加热混合物浓缩溶液; 用滤纸过滤浓缩的肉桂提取物; 并用0.45mum滤纸过滤第一次过滤的溶液。
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