japonica ) 아임계수 추출물을 유효성분으로 포함하는 화장료 조성물을 제공한다. 구체적으로, 본 발명은 유효성분인 다시마 아임계수 추출물은 1 MPa 내지 5 MPa 및 180℃ 내지 240℃의 아임계 조건에서 추출한 다시마 아임계수 추출물일 수 있다. 또한, 본 발명은 다시마로부터 항주름 활성이 가장 우수할 뿐만 아니라 추출수율도 가장 높은 다시마 아임계수 추출물의 제조방법에 관한 것이다. 상기 제조방법은 다시마를 분쇄하여 다시마 분쇄물을 제조하는 단계; 상기 다시마 분쇄물에 물을 첨가하는 단계; 및 상기 다시마 분쇄물 및 물을 3 MPa 및 180℃ 내지 240℃의 조건에서 반응시켜 다시마 아임계수 추출물을 제조하는 단계를 포함하는 주름 예방 또는 개선능을 가진 다시마 아임계수 추출물 제조방법일 수 있다. 본 발명의 제조방법에 의해 제조된 다시마 아임계수 추출물은 에스터라제 저해 활성 및 콜라겐 합성능 즉, 항주름 활성이 우수할 뿐만 아니라, 식품으로 사용되던 해조류와 가장 안전한 용매인 수용매를 이용하는 것으로 부작용 등에 대한 안전성도 보장되며, 우리나라 서해안지역에서 풍부하게 생산되는 해조류를 이용하여 간단한 방법으로 제조될 수 있어 경제적으로도 유리하고, 지역경제 활성화 및 지역 농어민 소득향상에도 이바지할 수 있으므로, 본 발명의 화장료 조성물은 산업적 측면에서 그 효과가 매우 클 것으로 기대된다.
Abstract:
The present invention relates to gel products using seaweed fulvescens and sea string and gel products thereby. By one embodiment of the present invention, the gel product using seaweed fulvescens and sea string comprises a step for washing the seaweed fulvescens and sea string; a step for drying the washed seaweed fulvescens and sea string in a dryer; a step for crushing the dried seaweed fulvescens and sea string and sieving the seaweed fulvescens and sea string to manufacture powder; a step for preparing jelly by putting the powder into a heated mixture including sediment, agar, and sugar and heating and stirring the mixture; a step for putting the jelly into a molding frame to mold the jelly; a step for drying the molded jelly at room temperature; and a step for cooling the dried jelly. the step for preparing jelly by putting the powder into a heated mixture including sediment, agar, and sugar and heating and stirring the mixture, honey and salt are additionally added. In the step for preparing jelly by putting the powder into a heated mixture including sediment, agar, and sugar and heating and stirring the mixture, based on 100 parts by weight of total jelly, the input amount of the powder is 0.5-2 parts by weight. In the step for preparing jelly by putting the powder into a heated mixture including sediment, agar, and sugar and heating and stirring the mixture, based on 100 parts by weight of total jelly, the input amount of seaweed fulvescens is 0.5 parts by weight and the input amount of the sea string is 1 parts by weight.
Abstract:
본 발명은 개꼬시래기 아임계수 추출물을 유효성분으로 포함하는 항염 조성물에 관한 것이다. 본 발명의 개꼬시래기 아임계수 추출물은 식용으로 사용되는 식물 및 가장 안전한 추출용매인 수용매를 이용한 추출물로서 부작용이나 안전성에 대한 문제가 없고, 염증과 관련된 NO 분비량 및 세포 생존률을 통하여 확인한 결과, 효과적으로 염증을 저해할 수 있는 것으로 확인되었다. 따라서, 상기 개꼬시래기 아임계수 추출물을 유효성분으로 포함하는 항염 조성물은 염증을 억제하여 염증과 관련된 질병을 치료 또는 예방하기 위한 항염증제 및 특정 제형 등과 관련하여 화장품 제조에 필수적으로 사용되는 성분 또는 외부환경으로 인한 피부의 염증을 효과적으로 억제할 수 있는 특징을 갖는 화장료 조성물 등에 응용될 수 있다.
Abstract:
A method for mass production of zoospores of Ecklonia stolonifera is provided to more stably perform the seed production of Ecklonia stolonifera and control the seed production period of Ecklonia stolonifera more freely by inducing the artificial maturation and apothecium formation of the immature Ecklonia stolonifera mummy. The zoospores of Ecklonia stolonifera is mass produced by culturing the immature Ecklonia stolonifera lobe or the lobe fraction cut therefrom, wherein the immature Ecklonia stolonifera is the biennial or greater; the lobe fraction is prepared by cutting the growing zone or the tip of Ecklonia stolonifera lobe, and is cultured under the conditions of the temperature of 19-21 deg. C, illuminance of 25-35 mumol m-2s-1 and illumination period of 10:14h(L:D); and the lobe or the lobe fraction cut therefrom is cultured for 90-110 days.
Abstract:
A cultivation method of Ecklonia stolonifera is provided to simplify the cultivation method by eliminating an outdoor seedling process and to increase the productivity. A cultivation method of Ecklonia stolonifera comprises a seed collection step for obtaining zoospore from the matured Ecklonia stolonifera dummy formed with apothecium; a step for obtaining the young leaf by cultivating zoospore; a step for cultivating young leaf; and a step for transplanting the cultivated young leaf in sea.
Abstract:
본발명은개꼬시래기아임계수추출물을유효성분으로포함하는비만치료, 개선또는예방용조성물에관한것이다. 특히, 본발명의유효성분인개꼬시래기아임계수추출물은지방전구세포(3T3-L1)의분화를저해하고, 지방세포의증식을억제하며, 지방대사물질의축적을억제할수 있으므로, 비만의치료, 개선및 예방효과가우수할뿐만아니라, 세포독성이없고, 천연물질로부터유래된것이므로부작용도문제되지아니하여비만의치료, 개선또는예방을위해널리사용할수 있다.
Abstract:
PURPOSE: Subcritical water extracts of laminaria japonica is provided to improve an esterase inhibition activity, a collagen synthesis capability and an anti-wrinkle activity and prevent side effects by using marine algae and an aqueous solvent, thereby having a marked effect in an industrial aspect. CONSTITUTION: A cosmetic composition for wrinkle protection or wrinkle improvement includes the subcritical water extracts of laminaria japonica which are extracted with the subcritical water of 3 MPa and 180-240°C. The cosmetic composition for wrinkle protection or wrinkle improvement adds water in crushed laminaria japonica and includes the subcritical water extracts of laminaria japonica which are characterized by being extracted under 3 MPa and 180-240°C as an active ingredient. A manufacturing method of the subcritical water extracts of laminaria japonica having a wrinkle protection activity and a wrinkle improvement activity comprises: a step of manufacturing crushed laminaria japonica by pulverizing laminaria japonica; a step of adding water in the crushed laminaria japonica; and a step of manufacturing the subcritical water extract of laminaria japonica by reacting the crushed laminaria japonica with water at a condition of 3 MPa and 180-240°C.
Abstract:
PURPOSE: A undaria peterseniana mass production method by inducing the regeneration and aging of undaria peterseniana free-living gametophytes is provided by securing the optimal condition for the regeneration and aging of the Undaria peterseniana free-living gametophytes. CONSTITUTION: Zoospores are released from an apothecium site of Undaria peterseniana. The released zoospores are cultured, and form gametophytes. Separate the gametophytes formed into female and male gametophytes. Culture the separated female and male gametophytes until the gametophytes reach the respective diameter of 3-10mm. Cut the cultured female and male gametophytes in the respective size of 50-400 micrometers. Culture the excised female and male gametophytes under the cell count of 3-25 cell/ind., the temperature condition of 5-20deg. C , the illumination of 5-40micromolm-2s-1, and the photoperiod of 10:14(Light:Dark)-14:10(L:D). Cross-fertilize the cultivated female and male gametophytes. Culture the gametophytes in a liquid culture medium of PESI(Provasoli`s enriched seawater media). Culture the female and male gametophytes until the gametophytes reach the diameter size of 5-6mm. Excise the female gametophytes in the size of 50-100 micrometers. Excise the male gametophytes in the size of 60-150 micrometers. Culture the female gametophytes under the cell count of 3-8 cell/ind., the temperature condition of 10-20 deg. C, the illumination of 15-30 micromolm-2s-1, and the photoperiod of 10:14(Light:Dark). Culture the male gametophytes under the cell count of 8-12 cell/ind., the temperature condition of 5-20 deg. C, the illumination of 15-25 micromolm-2s-1, and the photoperiod of 14:10(Light:Dark). [Reference numerals] (AA) Matured thallus of Undaria peterseniana; (BB) Rinsing the matured thallus, cut after washing, with sterilized seawater and ABM solution; (CC) Releasing zoospores at 10°C, 20umolm^-2s^-1, and 10:14h(L:D); (DD) 0.1ml zoospore solution; (EE) Separating the zoospores by dilution; (FF) Female gametophyte; (GG) Male gametophyte; (HH) Mass-producing clone gametophyte; (I1,I2) Cutting and regenerating every 20 days at 15°C, 20umolm^-2s^-1, and 10:14h(L:D); (JJ) Mixing the cut female and male gametophyte at the ratio of 1:1; (KK) Forming gamete for at least 15 days at 15°C, 20umolm^-2s^-1, and 14:10h(L:D); (LL) Forming young sporophyte for 40 days at 15°C, 60umolm^-2s^-1, and 14:10h(L:D)