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    참당귀에서분리한신규한펙틴질다당류와그분리정제방법및그의면역증강제로서의용도
    1.
    发明授权
    참당귀에서분리한신규한펙틴질다당류와그분리정제방법및그의면역증강제로서의용도 有权
    来自天竺葵的新型PECTIN型多糖，净化过程及其免疫学应用

    公开(公告)号：KR100252194B1

    公开(公告)日：2000-04-15

    申请号：KR1019970051930

    申请日：1997-10-10

    Applicant: 한국과학기술연구원

    Inventor： 김익환 ,  한상배 ,  안경섭 ,  이혜영 ,  최순자 ,  김환묵 ,  김영희 ,  이창우 ,  홍동호 ,  박선미

    IPC: A61K31/732 ,  A61K36/232

    CPC classification number: C08B37/0045 ,  A61K31/715 ,  C08B37/00

    Abstract: PURPOSE: A novel pectin polysaccharides(Angellan-I, Angellan-II) separated from Angelica gigas Nakai is provided, which contains galacturonic acid, arabinose and galactose, therefore shows immune-increasing effect and also can be used as an immune-increasing reagent in the basic study for immunology. CONSTITUTION: A process for the separation of novel pectin polysaccharides(Angellan-I, Angellan-II) from Angelica gigas Nakai comprises the steps of: pulverizing Angelica gigas Nakai root into pieces, adding water and extracting while circulating at 100deg.C for 1hr; adding 3times of ethanol to the residual solution, leaving at 4deg.C for 3hrs and centrifuging to get extracted precipitates; dissolving the ethanol extract in water to remove impurities, adding 3times of ethanol to the residual solution again, centrifuging and processing dialysis; freeze-drying the reagent to get crude polysaccharides; and separating the crude polysaccharides into acidic polysaccharides(Angellan I) and neutral polysaccharides(Angellan II) by dissolving, washing, centrifuging, doing dialysis and freeze-drying.

    Abstract translation: 目的：提供从当归纳西分离的新型果胶多糖（Angellan-I，Angellan-II），其含有半乳糖醛酸，阿拉伯糖和半乳糖，因此具有免疫增加作用，并且还可用作免疫增加试剂 免疫学的基础研究。 构成：从当归Nakai中分离出新型果胶多糖（Angellan-I，Angellan-II）的方法，包括以下步骤：将当归Nakai根粉碎成片，加水并在100℃循环提取1小时; 向剩余溶液中加入3倍的乙醇，在4℃下放置3h，离心得到提取的沉淀物; 将乙醇提取液溶解于水中去除杂质，再次向剩余溶液中加入3倍乙醇，离心和加工透析液; 冷冻干燥试剂得到粗多糖; 并通过溶解，洗涤，离心，进行透析和冷冻干燥将粗多糖分离成酸性多糖（Angellan I）和中性多糖（Angellan II）。

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