인간 리포코틴-1을 포함하는 신규한 유전자 운반체와 이를 이용한 인간 리포코틴-1의 제조방법

    公开(公告)号:KR1019950009839B1

    公开(公告)日:1995-08-29

    申请号:KR1019920007594

    申请日:1992-05-04

    Abstract: The plasmids pHT2 8505P (I) and pHT21 8506P (II) for human lipocortin-1 (III) are prepared by (1) isolating gene of (III) from plasmid pLCI-S KCTC 84189 (KR Appl. 89-223), (2) and adding trc promoter for IPTG inducing. (III) which inhibit for phospholipase A2 to prostaglandin and arachidonic acid formation is useful for anti-inflammatory and rheumatoid fever treatment.

    Abstract translation: 通过(1)从质粒pLCI-S KCTC 84189(KR Appl.89-223)分离(III)的基因((III))分离人脂细胞素-1(III)的质粒pHT2 8505P(I)和pHT21 8506P(II) 2)并加入诱导IPTG的trc启动子。 抑制磷脂酶A2对前列腺素和花生四烯酸形成的抑制剂(III)可用于抗炎和类风湿热治疗。

    참당귀에서분리한신규한펙틴질다당류와그분리정제방법및그의면역증강제로서의용도
    3.
    发明授权
    참당귀에서분리한신규한펙틴질다당류와그분리정제방법및그의면역증강제로서의용도 有权
    来自天竺葵的新型PECTIN型多糖,净化过程及其免疫学应用

    公开(公告)号:KR100252194B1

    公开(公告)日:2000-04-15

    申请号:KR1019970051930

    申请日:1997-10-10

    CPC classification number: C08B37/0045 A61K31/715 C08B37/00

    Abstract: PURPOSE: A novel pectin polysaccharides(Angellan-I, Angellan-II) separated from Angelica gigas Nakai is provided, which contains galacturonic acid, arabinose and galactose, therefore shows immune-increasing effect and also can be used as an immune-increasing reagent in the basic study for immunology. CONSTITUTION: A process for the separation of novel pectin polysaccharides(Angellan-I, Angellan-II) from Angelica gigas Nakai comprises the steps of: pulverizing Angelica gigas Nakai root into pieces, adding water and extracting while circulating at 100deg.C for 1hr; adding 3times of ethanol to the residual solution, leaving at 4deg.C for 3hrs and centrifuging to get extracted precipitates; dissolving the ethanol extract in water to remove impurities, adding 3times of ethanol to the residual solution again, centrifuging and processing dialysis; freeze-drying the reagent to get crude polysaccharides; and separating the crude polysaccharides into acidic polysaccharides(Angellan I) and neutral polysaccharides(Angellan II) by dissolving, washing, centrifuging, doing dialysis and freeze-drying.

    Abstract translation: 目的:提供从当归纳西分离的新型果胶多糖(Angellan-I,Angellan-II),其含有半乳糖醛酸,阿拉伯糖和半乳糖,因此具有免疫增加作用,并且还可用作免疫增加试剂 免疫学的基础研究。 构成:从当归Nakai中分离出新型果胶多糖(Angellan-I,Angellan-II)的方法,包括以下步骤:将当归Nakai根粉碎成片,加水并在100℃循环提取1小时; 向剩余溶液中加入3倍的乙醇,在4℃下放置3h,离心得到提取的沉淀物; 将乙醇提取液溶解于水中去除杂质,再次向剩余溶液中加入3倍乙醇,离心和加工透析液; 冷冻干燥试剂得到粗多糖; 并通过溶解,洗涤,离心,进行透析和冷冻干燥将粗多糖分离成酸性多糖(Angellan I)和中性多糖(Angellan II)。

Patent Agency Ranking